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1Soil microbial biomass carbon and nitrogen in forest ecosystems of Northeast China:a comparison between natural secondary forest and larch plantation显示文摘Aims Natural secondary forest(NSF)and larch plantation are two of the predominant forest types in Northeast China.However,how the two types of forests compare in sustaining soil quality is not well understood.This study was conducted to determine how natural secondary forest and larch plantation would differ in soil microbial biomass and soil organic matter quality.Methods Microbial biomass carbon(MBC),microbial biomass nitrogen(MBN),soil organic carbon(SOC)and total nitrogen(TN)in the 0-to 15-cm and 15-to 30-cm soil layers were investigated by making chemical and biological measurements in the montane region of eastern Liaoning Province,Northeast China,during the growing season of 2008 in stands of NSF and Larix olgensis plantation(LOP).Important Findings We found that soil MBC and MBN were significantly lower in the LOP than in the NSF.Both MBC and MBN declined significantly with increasing soil depth in the two types of stands.The ratios of MBC to SOC(MBC/SOC)and MBN to TN(MBN/TN)were also significantly lower in the LOP than in the NSF.Moreover,the values of MBC,MBC/SOC,and MBN/TN significantly varied with time and followed a similar pattern during the growing season,all with an apparent peak in summer.Our results indicate that NSF is better in sustaining soil microbial biomass and nutrients than larch plantation in the temperate Northeast China.This calls for cautions in large-scale conversions of the native forests to coniferous plantations as a forest management practice on concerns of sustaining soil productivity.Kai Yang Jiaojun Zhu Min Zhang Qiaoling Yan Osbert Jianxin Sun 2010Journal of Plant Ecology2010,3,3:44
2Comparison of Postoperative Pain Following Laser-assisted Subepithelial Keratectomy and Transepithelial Photorefractive Keratectomy:a Prospective,Random Paired Bilateral Eye Study显示文摘Purpose:To compare postoperative pain following laser-assisted subepithelial keratectomy(LASEK) and transepithelial photorefractive keratectomy(T-PRK, two-step surgery) and alleviate postoperative subjective pain.Methods:.Thirty patients(60 eyes) with myopia or myopic astigmatism were consecutively recruited into this prospective,randomized paired study..Patients underwent LASEK in one eye,and T-PRK in the other. The degree of pain was rated on a scale of 0-10 on postoperative days 1,2 and 3..Uncorrected visual acuity(UCVA) and subepithelial corneal haze were assessed at postoperative 1 and 3 months.Results:.The pain was relieved on the 4th postoperative day in all patients,.healing of corneal epithelium was observed at4-5 days after surgery and contact lenses were removed promptly.At postoperative 1 day,.the mean subjective pain score in the LASEK group was 3.2±1.88 and 4.43±1.61 in TPRK group(P=0.008).No significant difference was found between two groups on postoperative 2 and 3 days. At postoperative 3 months, the percentage of UCVA ≥0.8 in the LASEK group was 100% and 96.7% in the T-PRK group.(P =0.24),93.3% of patients in the LASEK with UCVA ≥1.0 and 90%in the T-PRK group(P=0.64). In the LASEK group, the value of corneal haze was 0.26 ±0.21 and 0.27 ±0.25 in the T-PRK group(P=0.877).Conclusion:.Good visual acuity was obtained in both groups at postoperative 3 months. Compared with those in the T-PRKgroup, patients undergoing had less discomfort in the LASEK group, which may be associated with corneal epithelial activity. The changing curve of subjective pain in the T-PRK group was relatively flat and stable at postoperative 3 days.Dongmei Wang Guangsheng Chen Liusong Tang Qiaoling Li 2014Eye Science2014,29,3:14
3Response of Pinus koraiensis seedling growth to different light conditions based on the assessment of photosynthesis in current and one-year-old needles显示文摘As one of the three major five-leaved pines in the northern hemisphere,Pinus koraiensis is the most important dominant tree species in the natural mixed-broadleaved Korean pine forests.However,the regeneration of P.koraiensis under the canopy of secondary forest stands is poor because of the light limitation.This study was conducted to understand how P.koraiensis seedlings adapt to different light intensities and what would be the optimum light level for their establishment and growth.Three repetition plots with four light intensities(15%,30%,60%and 100%of the natural incident irradiances,achieved by suspending layers of black nylon net above and surrounding the plots)were set up under natural climate conditions in a montane region in eastern Liaoning Province,Northeast China.A total of 80 P.koraiensis seedlings with similar height and root collar diameter were transplanted into four plots.After one year of acclimation to the specific light conditions,the seasonal variations of the photosynthetic variables and needle traits of the current and one-year-old needles,and the growth parameters were observed under four light intensities.The results indicated that:(1)The seedling at60%treatment exhibited the greatest growth,which agreed with the response of the light-saturated photosynthetic rates(Amax)and the dark respiration rate(Rd)in the current and one-year-old needles,i.e.,Rd at60%treatment was significantly lower than that at 100%treatment,but Amax did not differ between the seedlings at 100%and 60%treatments.(2)The P.koraiensis seedlings have a certain photosynthetic plasticity to adapt the light conditions by adjusting their needle traits and regulating the physiological processes,because Amax,Rd,light saturation point and compensation point,the needle mass area,needle nitrogen and chlorophyll contents were significantly(p<0.05)correlated with the light intensities.Especially,Amax at 100%and 60%treatments was significantly higher(p<0.05)than that at 30%and 15%treatments for both current and one-year-old needles.(3)The needles of different ages played a commutative role during the growing season,i.e.,the one-year-old needles played a major role for the photosynthesis in the early growing season;the current year needles did in the later growing season.This ensured the effective photosynthesis throughout the growing season.These findings suggest that P.koraiensis is the in-between heliophilous and shade-tolerant tree species at least for the seedlings up to 8 years.Jiaojun Zhu Kai Wang Yirong Sun Qiaoling Yan 2014Journal of Forestry Research2014,25,1:13
4Developing gene-tagged molecular markers for evaluation of genetic association of apple SWEET genes with fruit sugar accumulation显示文摘Sugar content is an important component of fruit quality.Although sugar transporters are known to be crucial for sugar accumulation,the role of genes encoding SWEET sugar transporters in fruit sugar accumulation remains elusive.Here we report the effect of the SWEET genes on fruit sugar accumulation in apple.A total of 25 MdSWEET genes were identified in the apple genome,and 9 were highly expressed throughout fruit development.Molecular markers of these 9 MdSWEET genes were developed and used for genotyping of 188 apple cultivars.The association of polymorphic MdSWEET genes with soluble sugar content in mature fruit was analyzed.Three genes,MdSWEET2e,MdSWEET9b,and MdSWEET15a,were significantly associated with fruit sugar content,with MdSWEET15a and MdSWEET9b accounting for a relatively large proportion of phenotypic variation in sugar content.Moreover,both MdSWEET9b and MdSWEET15a are located on chromosomal regions harboring QTLs for sugar content.Hence,MdSWEET9b and MdSWEET15a are likely candidates regulating fruit sugar accumulation in apple.Our study not only presents an efficient way of implementing gene functional study but also provides molecular tools for genetic improvement of fruit quality in apple-breeding programs.Qiaoling Zhen Ting Fang Qian Peng Liao Liao Li Zhao Albert Owiti Yuepeng Han 2018Horticulture Research2018,5,1:12
5Preparation of folate-conjugated starch nanoparticles and its application to tumor-targeted drug delivery vector显示文摘Anion starch nanoparticles (StNP) were prepared in water-in-oil microemulsion. Folate modi-fied with PEG was conjugated to the surface of StNP to obtain the folate-conjugated starch nanoparticles (FA-PEG/StNP). The average diameter of FA-PEG/ StNP determined by AFM and Zeta-Sizer apparatus was about 130 nm. Doxorubicin (DOX)-loaded FA- PEG/StNP was obtained via infiltrating combination. The result of UV spectrophotometer showed that the saturation concentration of DOX-loaded FA-PEG/ StNP was 28 μg/mg, which was effective for the con-trolled release of anticancer drug DOX. The cell ex-periments showed that the Lc50 of DOX-loaded FA-PEG/StNP and DOX-loaded StNP was higher than that of DOX, which indicates that FA-PEG/StNP and StNP can decrease the toxicity of DOX; while the lethal rate of DOX-loaded FA-PEG/StNP was 3 times that of DOX-loaded StNP with the same quantity of DOX, which indicates that FA in FA-PEG/StNP is ef-fective for improving the targeting function of nanoparticles, thus enhancing the inhibition effect of anticancer drug to cancer cell. This work demon-strates that the FA-PEG/StNP system is a potentially useful system for the targeted delivery of anticancer drug DOX.XlAO Suyao TONG Chunyi LIU Xuanming YU Danmi LIU Qiaoling XUE Changgang TANG Dongyin ZHAO Lijian 2006Chinese Science Bulletin2006,51,14:12
6Effect of deformation and cooling rate on the transformation behavior and microstructure of X70 steels显示文摘The effects of the deformation in the non-recrystallization region of austenite and the cooling rate on the transformation behavior and microstructure of low-carbon low-alloy steel for pipeline application were studied on the thermal-mechanical simulator Gleeble-1500. It was shown that an increase in deformation amount can greatly increase the nucleation site of ferrite when deformed in the non-recrystallization region of austenite, and an increase in nucleation ratio can greatly refine grains. When the cooling rate is accelerated, the driving force of nucleation is increased and the nucleation rate also improves. Ultra-refine grains can be obtained by controlled rolling. The high density of ferrite nucleus, which forms along the austenite grain boundary, twin interface, and deforma- tion band are introduced in the matrix of austenite by the control of hot rolling, after which the microstructure can be refined. It was found that the acicular ferrite has a very fine sub-structure, high dislocation density, and a thin slab with ultra-fine grains. Small M/A islands and cementite are precipitated on the matrix of the slabs by the analysis technique of TEM and SEM.Zhiping Zhao Zhenmin Wang Hongmei Zhang Lifeng Qiaol 2007Journal of University of Science and Technology Beijing2007,14,5:11
7Notoginsenoside Ft1 acts as a TGR5 agonist but FXR antagonist to alleviate high fat diet-induced obesity and insulin resistance in mice显示文摘Obesity and its associated complications are highly related to a current public health crisis around the world.A growing body of evidence has indicated that G-protein coupled bile acid(BA) receptor TGR5(also known as Gpbar-1) is a potential drug target to treat obesity and associated metabolic disorders.We have identified notoginsenoside Ftl(Ftl) from Panax notoginseng as an agonist of TGR5 in vitro.However,the pharmacological effects of Ftl on diet-induced obese(DIO) mice and the underlying mechanisms are still elusive.Here we show that Ftl(100 mg/100 diet) increased adipose lipolysis,promoted fat browning in inguinal adipose tissue and induced glucagon-like peptide-1(GLP-1) secretion in the ileum of wild type but not Tgr5^(-/-) obese mice.In addition,Ftl elevated serum free and taurineconjugated bile acids(BAs) by antagonizing Fxr transcriptional activities in the ileum to activate Tgr5 in the adipose tissues.The metabolic benefits of Ftl were abolished in Cyp27 al^(-/-) mice which have much lower BA levels.These results identify Ftl as a single compound with opposite activities on two key BA receptors to alleviate high fat diet-induced obesity and insulin resistance in mice.Lili Ding Qiaoling Yang Eryun Zhang Yangmeng Wang Siming Sun Yingbo Yang Tong Tian Zhengcai Ju Linshan Jiang Xunjiang Wang Zhengtao Wang Wendong Huang Li Yang 2021Acta Pharmaceutica Sinica B2021,11,6:10
8Bile acids evoke placental inflammation by activating Gpbar1/NF-κB pathway in intrahepatic cholestasis of pregnancy显示文摘Intrahepatic cholestasis of pregnancy(ICP)is a cholestatic disorder with potentially deleterious consequences for fetuses.Although a clear correlation between the elevated levels of maternal serum bile acids and deficient fetal outcome has been established in clinical practice,the underlying mechanisms remain elusive.Herein,we report that bile acids induce NF-κB pathway activation via G protein-coupled bile acid receptor 1(Gpbar1),with consequent upregulation of inflammatory genes in trophoblasts,leading to aberrant leukocyte infiltration and inflammation in placenta.Ursodeoxycholic acid(UDCA),a drug used clinically to treat ICP,competes with other bile acids for binding with Gpbar1 and thus inhibits bile acid-induced inflammatory response in trophoblasts and improves fetal survival in pregnant rats with obstructive cholestasis.Notably,inhibition of NF-κB by andrographolide is more effective than UDCA in benefiting placentas and fetuses.Thus,anti-inflammation therapy targeting Gpbar1/NF-κB pathway could be effective in suppressing bile acid-induced inflammation and alleviating ICP-associated fetal disorders.YouHua Zhang YouDong Pan ChangDong Lin YaJuan Zheng Hao Sun HaiLong Zhang JunLei Wang MengYa Yuan Tao Duan QiaoLing Du JianFeng Chen 2016Journal of Molecular Cell Biology2016,8,6:8
9Investigation of CFD Calculation Method of a Centrifugal Pump with Unshrouded Impeller显示文摘Currently, relatively large errors are found in numerical results in some low-specific-speed centrifugal pumps with unshrouded impeller because the effect of clearances and holes are not accurately modeled. Establishing an accurate analytical model to improve performance prediction accuracy is therefore necessary. In this paper, a three-dimensional numerical simulation is conducted to predict the performance of a low-specific-speed centrifugal pump, and the modeling, numerical scheme, and turbulent selection methods are discussed. The pump performance is tested in a model pump test bench, and flow rate, head, power and efficiency of the pump are obtained. The effect of taking into consideration the back-out vane passage, clearance, and balance holes is analyzed by comparing it with experimental results, and the performance prediction methods are validated by experiments. The analysis results show that the pump performance can be accurately predicted by the improved method. Ignoring the back-out vane passage in the calculation model of unshrouded impeller is found to generate better numerical results. Further, the calculation model with the clearances and balance holes can obviously enhance the numerical accuracy. The application of disconnect interface can reduce meshing difficulty but increase the calculation error at the off-design operating point at the same time. Compared with the standard k–ε, renormalization group k–ε, and Spalart–Allmars models, the Realizable k–ε model demonstrates the fastest convergent speed and the highest precision for the unshrouded impeller flow simulation. The proposed modeling and numerical simulation methods can improve the performance prediction accuracy of the low-specific-speed centrifugal pumps, and the modeling method is especially suitable for the centrifugal pump with unshrouded impeller.WU Dazhuan YANG Shuai XU Binjie LIU Qiaoling WU Peng WANG Leqin 2014Chinese Journal of Mechanical Engineering2014,27,2:7
10Preparation of chitosan rods with excellent mechanical properties: One candidate for bone fracture internal fixation显示文摘Chitosan (CS) is one promising material as a temporary mechanical supporter for bone fracture internal fixation.In our previous work,we successfully fabricated CS rods through one in situ precipitation route.But bending strength and bending modulus of CS rods need to be improved to match the commercially available devices used for bone fracture internal fixation.In this research,CS rods were reinforced effectively through cross-linking reaction by using glutaraldehyde as the coupling reagent.Schiff’s base was detected by FTIR due to the chemical reaction between amino groups and aldehyde groups.Crystal plane space of CS rods became small during the formation of network structure.Microstructure was observed by SEM,indicating that layer-by-layer structure became much tighter after cross-linking reaction,and cracks in one layer turned around when they reached another layer to absorb energy.Bending strength and bending modulus of cross-linked CS rods could reach 186.3 MPa and 5.17 GPa,respectively.Compared with uncross-linked CS rods,they are increased by 101.6% and 26.1%,respectively.As a result,mechanical properties of CS rods are equivalent to the commercially available biodegradable devices.CS rods with excellent mechanical properties are a good candidate for bone fracture internal fixation.WANG ZhengKe HU QiaoLing WANG YouXiang 2011Science China Chemistry2011,54,2:7
11Sensory and chemical assessment of silver pomfret (Pampus argenteus) treated with Ginkgo biloba leaf extract treatment during storage in ice显示文摘This study investigated the physical(L*,a*,b*,texture profile analyses,pH),chemical(TVB-N,K value and TBA),microbiological,amino acid content,and flavor effects that Gingko biloba leaf extract(GBLE)had on silver pomfret(Pampus argenteus)stored at 4±1C in ice for 18 days.Fresh pomfret samples were obtained directly from the local fish market and transported to the laboratory with ice immediately.After being gutted,washed,filleted and trimmed in a water-ice mixture,samples were treated with different concentrations of GBLE(0.0 mg/mL,2.5 mg/mL,5.0 mg/mL,10.0 mg/mL)and packaged in Polyethylene bag,then stored in a refrigerator at 4±1C with ice.The results show that the shelf-life of untreated(0.0 mg/mL)pomfret samples was 8e9 days compared to 14e15 days for the GBLE1(2.5 mg/mL)treated group.The assessment results showed that different concentrations of GBLE had variable effects on preserving the texture parameters of acceptability limit,inhibit lipid oxidation,protein degradation,and microorganism growth.2.5 mg/mL of GBLE was the best for the preservation of pomfret during storage in ice.Therefore,there is potential use for GBLE as a preservative to extend the shelf-life of pomfret during chilled storage in ice.Weiqing Lan Xu Che Qiaoling Xu Ting Wang Ruoyuan Du Jing Xie Min Hou Han Lei 2018Aquaculture and Fisheries2018,3,1:5
12Causal effects of shelter forests and water factors on desertification control during 2000–2010 at the Horqin Sandy Land region, China显示文摘The Horqin Sandy Land(HSL), the largest sandy land in the semi-arid agro-pastoral ecotone of Northeast China, has been subject to desertification during the past century. In response, and to control the desertification,government implemented the Three-North Shelter/Protective Forest Program, world's largest ecological reforestation/afforestation restoration program. The program began in1978 and will continue for 75 years until 2050. Understanding the dynamics of desertification and its driving forces is a precondition for controlling desertification.However, there is little evidence to directly link causal effects with desertification process(i.e., on the changing area of sandy land) because desertification is a complex process,that can be affected by vegetation(including vegetation cover and extent of shelter forests) and water factors such as precipitation, surface soil moisture, and evapotranspiration.The objectives of this study were to identify how influencing factors, especially shelter forests, affected desertification in HSL over a recent decade. We used Landsat TM imagery analysis and path analysis to identify the effects of spatiotemporal changes in water and vegetation parameters during2000–2010. Desertification was controlled during the study period, as indicated by a decrease in desert area at a rate of163.3 km2year-1and an increase in the area with reduced intensity or extent of desertification. Total vegetation cover in HSL increased by 10.6 % during the study period and this factor exerted the greatest direct and indirect effects on slowing desertification. The contribution of total vegetation cover to controlling desertification increased with the intensity of desertification. On slightly and extremely severe desertified areas, vegetation cover contributed 5 and 42 % of the desertification reduction, respectively. There were significant correlations between total vegetation cover and water conditions(i.e., evapotranspiration and precipitation)and the area of shelter forests(P \ 0.0001), in which water conditions and the existence of shelter forests contributed49.7 and 12.8 % to total vegetation cover, respectively. The area of shelter forests increased sharply due to program efforts, but only shrub forests had significant direct effects on reducing the area of desertification categorized as slightly desertified. The reason for the lack of direct effect of increased arbor forests(accounting for 95.3 % of the total increase in shelter forests) on reducing desertification might be that the selected arbor species were not suited to water conditions(low precipitation, high evapotranspiration) prevailing at HSL. The establishment of shelter forests aided control of desertification in the HSL region, but the effect was less than expected. Effective control of desertification in the HSL region or other similar sandy areas will require greater improvements in vegetation cover. In particular,shrub species should be selected for plantation with reference to their potential to survive and reproduce in the harsh climatic and weather conditions typical of desertified areas.Qiaoling Yan Jiaojun Zhu Xiao Zheng Changjie Jin 2015Journal of Forestry Research2015,26,1:5
13Rod-shaped thiocyanate-induced abnormal band gap broadening in SCN^- doped CsPbBr3 perovskite nanocrystals显示文摘Yongbing Lou Yandan Niu Dongwen Yang Qiaoling Xu Yuhang Hu Ying Shen Jing Ming Jinxi Chen Lijun Zhang Yixin Zhao 2018Nano Research2018,11,5:5
14On conformal vector fields on Randers manifolds显示文摘In this paper,we study conformal vector fields on a Randers manifold with certain curvature properties.In particular,we completely determine conformal vector fields on a Randers manifold of weakly isotropic flag curvature.SHEN ZhongMin XIA QiaoLing 2012Science China Mathematics2012,55,9:5
15Integrated evaluation of payments for ecosystem services programs in China:a systematic review显示文摘Introduction:During the past two decades,payments for ecosystem services(PES)program has become a popular conservation paradigm for realigning socioeconomic costs and benefits among different stakeholders.As billions of investment flows into the natural capital pool,there is growing interest to understand the ecological,economic,and social outcomes of PES programs.China is one of the countries that extensively implements PES programs.Although there is a growing interest to perform impact evaluation of China’s massive PES programs,it is unclear that what existing literature has done,has not done,and should do in the future.Therefore,to guide further research and practices,we conduct a systematic review of studies on China’s PES programs.Results:Our review shows that there are growing impact evaluation studies of PES programs in China.However,the spatial and ecosystem distributions of existing studies are quite uneven.Most case studies were poorly designed,rarely quantified,and evaluated without sophisticated methods.Among the three dimensions of ecological effectiveness,economic efficiency,and social equity,economic efficiency is the least studied.Discussion and Conclusion:We further discuss the challenges and opportunities and provide insights for future research.To improve the understanding and management of natural capital,we call for mainstreaming impact evaluation of ecosystem service policies in China and beyond following the state-of-art procedures.Wu Yang Qiaoling Lu 2018Ecosystem Health and Sustainability2018,4,3:5
16Longitudinal Alterations of Local Spontaneous Brain Activity in Parkinson's Disease显示文摘We used resting-state fMRI to evaluate longitudinal alterations in local spontaneous brain activity in Parkinson's disease(PD) over a 2-year period. Data were acquired from 23 PD patients at baseline and follow-up,and 27 age-and sex-matched normal controls. Regional homogeneity(ReHo) and voxel-based-morphometry(VBM) were used to identify differences in local spontaneous brain activity and grey matter volume. With disease progression, we observed a progressive decrease in ReHo in the sensorimotor cortex, default-mode network, and left cerebellum, but increased ReHo in the supplementary motor area, bilateral temporal gyrus, and hippocampus.Moreover, there was a significant positive correlation between the rates of ReHo change in the left cerebellum and the rates of change in the Unified Parkinson's Disease Rating Scale-III scores. VBM revealed no significant differences in the grey matter volume among the three sets of acquisitions. We conclude that ReHo may be a suitable non-invasive marker of progression in PD.Qiaoling Zeng Xiaojun Guan Jason C.F.Law Yan Lun Zhujing Shen Tao Guo Min Xuan Quanquan Gu Xiaojun Xu Min Chen Minming Zhang 2017Neuroscience Bulletin2017,33,5:4
17Effects of Airflow Field on Droplets Diameter inside the Corrugated Packing of a Rotating Packed Bed显示文摘Rotating packing bed(RPB) has a better mixing performance than traditional mixers and shows potential application in the petroleum industry. However, acquisition of information about the mixing process directly through experiments is difficult because of the compact structure and complex multiphase flow pattern in RPB. To study the mixing characteristic, Fluent, the computational fluid dynamics(CFD) software, was used to explore the effect of airflow field on droplet diameter. For conducting calculations, the gas-liquid two-phase flow inside the packing was simulated with the RNG k-ε turbulence model and the Lagrange Discrete Phase Model(DPM), respectively. The numerical calculation results showed that coalescence and breakup of droplets can take place in the gas phase flow inside the packing and can be strengthened with increased rotating speed, thereby leading to the enlargement of the average diameter.Xu Chengcheng Jiao Weizhou Liu Youzhi Guo Liang Yuan Zhiguo Zhang Qiaoling 2014China Petroleum Processing & Petrochemical Technology2014,16,4:4
18Soil pore identification with the adaptive fuzzy C-means method based on computed tomography images显示文摘The complex geometry and topology of soil is widely recognised as the key driver in many ecological processes. X-ray computed tomography (CT) provides insight into the internal structure of soil pores automatically and accurately. Until recently, there have not been methods to identify soil pore structures. This has restricted the development of soil science, particularly regarding pore geometry and spatial distribution. Through the adoption of the fuzzy clustering theory and the establishment of pore identification rules, a novel pore identification method is described to extract pore structures from CT soil images. The robustness of the adaptive fuzzy C-means method (AFCM), the adaptive threshold method, and Image-Pro Plus tools were compared on soil specimens under different conditions, such as frozen, saturated, and dry situations. The results demonstrate that the AFCM method is suitable for identifying pore clusters, especially tiny pores, under various soil conditions. The method would provide an optional technique for the study of soil micromorphology.Yue Zhao Qiaoling Han Yandong Zhao Jinhao Liu 2019Journal of Forestry Research2019,30,3:4
19Effect of size and crystalline phase of TiO2 nanotubes on cell behaviors: A high throughput study using gradient TiO2 nanotubes显示文摘The research of TiO2 nanotubes(TNTs)in the field of biomedicine has been increasingly active.However,given the diversity of the nanoscale dimension and controversial reports,our understanding of the structure-property relationships of TNTs is not yet complete.In this paper,gradient TNTs with a wide diameter range of 20-350 nm were achieved by bipolar electrochemistry and utilized for a thorough high-throughput study of the effect of nanotube dimension and crystalline phase on protein adsorption and cell behaviors.Results indicated that protein adsorption escalated with nanotube dimension whereas cell proliferation and differentiation are preferred on small diameter(<70 nm)nanotubes.Large diameter anatase nanotubes had higher adsorption of serum proteins than as-prepared ones.But only as-prepared small diameter nanotubes presented slightly higher cell proliferation than corresponding annealed nanotubes whereas there was no discernible difference between as-prepared and annealed nanotubes on cell differentiation for the entire gradient.Those findings replenish previous research about how cell responses to TNTs with a wide diameter range and provide scientific guidance for the optimal design of biomedical materials.Yanran Li Si Wang Yuanjun Dong Ping Mu Yun Yang Xiangyang Liu Changjian Lin Qiaoling Huang 2020Bioactive Materials2020,5,4:3
20A super pan-genomic landscape of rice显示文摘Pan-genomes from large natural populations can capture genetic diversity and reveal genomic complexity.Using de novo longread assembly,we generated a graph-based super pan-genome of rice consisting of a 251-accession panel comprising both cultivated and wild species of Asian and African rice.Our pan-genome reveals extensive structural variations(SVs)and gene presence/absence variations.Additionally,our pan-genome enables the accurate identification of nucleotide-binding leucine-rich repeat genes and characterization of their inter-and intraspecific diversity.Moreover,we uncovered grain weight-associated SVs which specify traits by affecting the expression of their nearby genes.We characterized genetic variants associated with submergence tolerance,seed shattering and plant architecture and found independent selection for a common set of genes that drove adaptation and domestication in Asian and African rice.This super pan-genome facilitates pinpointing of lineage-specific haplotypes for trait-associated genes and provides insights into the evolutionary events that have shaped the genomic architecture of various rice species.Lianguang Shang Xiaoxia Li Huiying He Qiaoling Yuan Yanni Song Zhaoran Wei Hai Lin Min Hu Fengli Zhao Chao Zhang Yuhua Li Hongsheng Gao Tianyi Wang Xiangpei Liu Hong Zhang Ya Zhang Shuaimin Cao Xiaoman Yu Bintao Zhang Yong Zhang Yiqing Tan Mao Qin Cheng Ai Yingxue Yang Bin Zhang Zhiqiang Hu Hongru Wang Yang Lv Yuexing Wang Jie Ma Quan Wang Hongwei Lu Zhe Wu Shanlin Liu Zongyi Sun Hongliang Zhang Longbiao Guo Zichao Li Yongfeng Zhou Jiayang Li Zuofeng Zhu Guosheng Xiong Jue Ruan Qian Qian 2022Cell Research2022,32,10:3
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