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| 1 | Lymphopenia predicts disease severity of COVID-19:a descriptive and predictive study显示文摘Dear Editor,An outbreak of an unknown infectious pneumonia has recently occurred in Wuhan,China.1 The pathogen of the disease was quickly identified as a novel coronavirus(SARS-CoV-2,severe acute respiratory syndrome coronavirus 2),and the disease was named coronavirus disease-19(COVID-19).2 The virus has so far caused 78,959 confirmed cases and 2791 deaths in China according to the reports of government.COVID-19 has been spreading in many countries such as Japan,Korea,Singapore,Iran,and Italia. | Li Tan Qi Wang Duanyang Zhang Jinya Ding Qianchuan Huang Yi-Quan Tang Qiongshu Wang Hongming Miao | 2020 | Signal Transduction and Targeted Therapy2020，5，1： | 4 |
| 2 | RNAi抑制Bmi-1基因表达对Jurkat细胞的影响(英文)显示文摘Objective: The aim of our study was to investigate the effects of down-regulation Bmi-1 by RNA interference (RNAi) in T Lymphocytic leukemia Jurkat cells. Methods: Two complementary oligonucleotide strands were synthesized based on the siRNA sequence targeting Bmi-1 gene. After annealing, siRNA strands were recombined into the pRNAT-U6.2 vector, and then DNA sequencing was carried out following transformation and amplification. The recombinant was transfected into Jurkat cells with liposomes. Positive colonies were obtained through G418 selection. The mRNA and protein expressions of Bmi-1 were detected by RT-PCR and Western-blot, respectively. Effects of Bmi-1 silence on cell proliferation, cell cycle and cell aging of Jurkat cells were detected by MTT assay, flow cytometry, colony formation assay and SA-β-Gal staining, respectively. Results: The siRNA recombinant targeting Bmi-1 gene was successfully constructed. All three siRNA recombinants could significantly inhibit the expression of Bmi-1. The siRNA targeting 825nt-843nt (GACCAGACCACTACT GAAT) has the strongest inhibitory effect on Bmi-1 expression, with almost complete inhibition on Bmi-1 mRNA and protein expressions. Compared with the non-transfection group and the empty vector group, growth velocity and colony formation ability were significantly decreased, while the proportion of cells in G1 phase and the percentage of senile cells were signifi-cantly increased in highly transfected group (P < 0.05). Conclusion: Down-regulation Bmi-1 by RNA interference (RNAi) could significantly inhibit the growth of Jurkat cells in vitro. | Shangen Zheng Qibin Jing Yaqiong Zheng Yinjuan Ding Qianchuan Huang Guoqiang Zhao | 2012 | The Chinese-German Journal of Clinical Oncology2012，11，12： | 0 |
| 3 | Correction:Lymphopenia predicts disease severity of COVID-19:a descriptive and predictive study显示文摘Correction to:Signal Transduction and Targeted Therapy http://gffzzd3cc09b8251d45dfsxvcwcu9bvfp96cuu.ffgz.tsg.suse.edu.cn/10.1038/s41392-020-0148-4,published online 27 March 2020 Since the publication of this article,1 we noticed a minor mistake in the reference that needs to be corrected. | Li Tan Qi Wang Duanyang Zhang Jinya Ding Qianchuan Huang Yi-Quan Tang Qiongshu Wang d Hongming Miao | 2020 | Signal Transduction and Targeted Therapy2020，5，1： | 0 |
| 4 | Consecutive Monitoring of Interleukin-6 Is Needed for COVID-19 Patients显示文摘Dear Editor,The world is facing an immense challenge in the fight against coronavirus disease 2019(COVID-19),which is caused by severe acute respiratory syndrome coronavirus 2(SARS-Co V-2)(Gorbalenya et al.2020).To date,tens of millions of people worldwide have been infected with SARS-Co V-2,and more than a million have died,and the situation continues. | Xiaohua Chen Juan Zhou Chen Chen Baidong Hou Ashaq Ali Feng Li Zhaolin Hua Yingtao Wu Qin Yang Min Chen Rong Zhang Qianchuan Huang Jinya Ding Xian-En Zhang Dong Men | 2021 | Virologica Sinica2021，36，5： | 0 |
| 5 | 过表达人血管生成素样蛋白4对食管癌EC9706细胞生长的影响(英文)显示文摘Objective:The aim of the study was to construct the eukaryotic expression vector of human angiopoietin-like protein 4(ANGPTL4) and observe the effect of ANGPTL4 overexpression on the growth of esophageal carcinoma EC9706 cells.Methods:Total RNA was extracted from normal hepatic tissue,and ANGPTL4 cDNA was amplified by RT-PCR.The PCR product was doubly digested by XbaI and SalI,and then recombined into eukaryotic expression vector.Then,pIRES-GFP-ANGPTL4 was obtained by G418 selection,then pIRES-GFP-ANGPTL4 and pIRES-GFP were transfected into EC9706 cells with lipidosome-packaged method.Meanwhile,the transfected cells were selected by G418,and then stable transfected cell lines were obtained.ANGPTL4 mRNA levels,the cell cycles and growth curves of EC9706 cells in experiment group(transfected with pIRES-GFP-ANGPTL4),empty vector group(transfected with pIRES-GFP) and blank control group(EC9706 cells without transfection) were detected with RT-PCR,flow cytometry and MTT methods,respectively.Results:Eukaryotic ANGPTL4 expression vector pIRES-GFP-ANGPTL4 was successfully constructed.The ANGPTL4 mRNA level(0.21 ± 0.03) in experiment group was significantly higher than that of the empty vector group(0.04 ± 0.008) and the blank control group(0.05 ± 0.007),with significant differences(P < 0.01).The proportion of cells in S phase in experiment group was significantly different with those of the other two groups(P < 0.05).The cell growth of EC9706 cells in experiment group was slower than those of the other two groups.From the third day,the differences began to be significant.Conclusion:ANGPTL4 overexpression in esophageal carcinoma EC9706 cells could inhibit the growth of EC9706 cells. | Shangen Zheng Fang Wang Yinjuan Ding Lifang Zhang Qianchuan Huang Guoqiang Zhao | 2010 | The Chinese-German Journal of Clinical Oncology2010，9，2： | 0 |
| 6 | Production Line Capacity Planning Concerning Uncertain Demands for a Class of Manufacturing Systems with Multiple Products显示文摘In this paper, we study a class of manufacturing systems which consist of multiple plants and each of the plants has capability of producing multiple distinct products. The production lines of a certain plant may switch between producing different kinds of products in a time-sharing mode. We optimize the capacity configuration of such a system s production lines with the objective to maximize the overall profit in the capacity planning horizon. Uncertain demand is incorporated in the model to achieve a robust configuration solution. The optimization problem is formulated as a nonlinear polynomial stochastic programming problem, which is difficult to be efficiently solved due to demand uncertainties and large search space. We show the NP-hardness of the problem first, and then apply ordinal optimization(OO) method to search for good enough designs with high probability. At lower level, an mixed integer programming(MIP) solving tool is employed to evaluate the performance of a design under given demand profile. | Hao Liu Qianchuan Zhao Ningjian Huang Xiang Zhao | 2015 | IEEE/CAA Journal of Automatica Sinica2015，2，2： | 0 |
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