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| 1 | Effects of 5% Biological Compound Premix on Milk Perform ance and Quality in Dairy Cow显示文摘This study was to investigate the effect of biological compound premix on the performance and milk quality in dairy cows. Twenty Holstein cows,with the similar age,parity,lactating month,gestation period,milk quantity and body weight,were randomly divided into two groups. The cows in two groups were fed with either common compound premix as control or 5% biological compound premix as treatment group. Two treatments contained same basal diet but different compositions of premix. The feeding experiment lasting 74 days displayed that daily milk quantity in treatment group was significantly higher than control group( P < 0. 05).The milk fat percentage was increased significantly in treatment group with the extended lactation time than control group( P < 0. 05). The milk protein and solids percentage were raised,but no significant difference was found between the two treatments( P > 0. 05). The results indicated that 5% biological compound premix supplied in basal diet could facilitate to increase the milk performance and to ameliorate the milk quality of dairy cows. | Qiao Fuqiang Qiao Limin Yao Hua | 2014 | Animal Husbandry and Feed Science2014，6，3： | 1 |
| 2 | Effect of Different Thawing Methods on Post-thaw Motility of Cow Semen in Cryopreservation Straws显示文摘The study was to investigate the effects of different thawing temperatures(5,15,40,75,90 ℃) and times(1- 120 s) on properties of post-thaw cow semen by detecting frozen-thawed semen motility,acrosome integrity and tail membrane integrity,further obtaining the optimal thawing method of straw frozen semen from dairy cow. The results showed that(1) Thawing of the straw frozen semen of dairy cow at 75 ℃ for 3 s yielded the highest semen motility,followed by 40 ℃for 20 s,and the least by low temperature 5 ℃ and room temperature 15 ℃ with a semen motility of 0. 3,moreover thawing at high temperature 90 ℃ was not suitable for large scale production due to the difficult control of the temperature;(2) The acrosome intact rate and plasma membrane integrity rate of semens thawed at90 ℃ were remarkably lower than that at 40 ℃ and 75 ℃ significantly(P < 0. 05),while its semen malformation rate was significantly higher than that at 40 ℃and 75 ℃(P < 0. 05);(3) The Survival time of semens at 37 ℃ varied largely among different thawing temperature,in detail by 40 ℃ > 75 ℃ > 90 ℃. In practice,the thawing method of straw frozen semen of dairy cow should be selected according to the specific circumstance and inseminated immediately,with the recommended condition of thawing at 75 ℃ for 3 s. If the thawed semen could not be inseminated immediately,the thawing should be performed at 20 s for 40 ℃to maintain the motility for a longer term. | Qiao Limin Guo Tong Qiao Fuqiang Yao Hua Xiao Xishan Hou Yinxu Fu Jingtao Ren Kang | 2014 | Animal Husbandry and Feed Science2014，6，4： | 1 |
| 3 | Effects of Transport,Storage Temperature and Time on Parthenogenetically Developmental Competence of Sheep Oocyte显示文摘This study was to investigate the effects of transport,storage temperature and time on parthenogenetical developmental competence of sheep oocyte. The ovaries were collected and randomly immersed in physiological saline at( 10- 15) ℃,( 20- 25) ℃ and( 30- 35) ℃ and transported to the laboratory for culture.They were divided into three groups randomly in an optimized transport temperature,kept in physiological saline at 4 ℃,( 14- 18) ℃ and( 25- 30) ℃ respectively; 15- 17 h later,the ovaries were cutured for the maturation and parthenogenetic activation of oocytes. The results showed that the optimal transport temperature of ovaries was 20- 25 ℃,when the maturation rate and blastocysts rate reached 67. 44% and 35. 93% respectively. Incubating sheep ovaries at( 14- 18) ℃for( 15- 17) h did not reduce the maturation rate( 61. 81%) and blastocysts rate( 29. 03%) of oocyte significantly. However,incubating sheep ovaries at 4 ℃or( 25- 30) ℃ significantly reduced the maturation rate( 41. 90%,18. 40%) and cleavage rate( 9. 09%,13. 04%),and the ovaries could not develop into blastocysts at these two temperature ranges. It can be concluded that the optimal transport temperature of the sheep ovaries is 20- 25 ℃,and incubation at 14- 18 ℃overnight in vivo does not affect the developmental competence of oocytes. | Qiao Limin Qiao Fuqiang Guo Tong Yao Hua Zhang Jinghe Xiao Xishan Hou Yinxu Guan Weijun | 2014 | Animal Husbandry and Feed Science2014，6，3： | 0 |
| 4 | Effects of Storage Temperature on the Effective Activity of Straw Frozen Semen after Thawing显示文摘The aim was to discuss the optimal storage environment and proper insemination time after thawing of 0. 25 m L straw frozen semen. Straw frozen semen was thawed at 40 ℃ for 20 s,and then stored at 0- 4 ℃,14- 16 ℃,25- 27 ℃ for 2,4,6,8 and 10 h,respectively. The sperm motility was detected. After thawing,semen was stored at 0- 4 ℃ and 14- 16 ℃ for 10 h. Their sperm motilities( 0. 434 ± 0. 016 7 and 0. 423 ± 0. 019 6) had no significant differences( P> 0. 05) with initial thawing motility( 0. 441 ± 0. 030). Sperm motility reduced as the storage time prolonged at 25- 27 ℃. Sperm motility after 6 h had significant differences with that of initial thawing motility( P < 0. 05),and sperm motilities after 8 and 10 h showed extremely significant differences( P < 0. 01).Thus,sperm motility after thawing was still very high after stored at 0- 4 ℃ and 14- 16 ℃ within 10 h,which met the requirements for insemination. Under this temperature and time ranges,sperm could be carried over long distances,which had small effects on sperm quality and reached the expected insemination effects.However,under the temperature of 25- 27 ℃,semen should be used for insemination within 6 h after thawing. | Qiao Limin Ma Jianmin Qiao Fuqiang Yao Hua Xiao Xishan Hou Yinxu Fu Jingtao Ren Kang | 2015 | Animal Husbandry and Feed Science2015，7，4： | 0 |
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