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| 1 | Ectopic expression of antifreeze protein gene from Ammopiptanthus nanus confers chilling tolerance in maize显示文摘Improved chilling tolerance is important for maize production. Previous efforts in transgenics and marker-assisted breeding have not achieved practical results. In this study, the antifreeze protein(AnAFP) from the super-xerophyte Ammopiptanthus nanus was aligned to KnS-type dehydrins.Phosphorylation in vitro and subcellular localization showed that AnAFP was phosphorylated by maize casein kinase II and translocated from nucleus to cytoplasm under chilling stress. AnAFP also increased lactate dehydrogenase activity. A parent line of commercial maize hybrids was transformed with the AnAFP gene. Based on thermal asymmetric interlaced PCR, one hemizygous and two homozygous integration sites were identified in one T_(1) line. Ectopic expression of AnAFP in transgenic lines was confirmed by quantitative real-time PCR, RNA-seq, and Western blotting. After chilling treatment, the transgenic lines showed significantly improved phenotype, higher kernel production, survival rate and biomass, and lower relative electrolyte leakage and superoxide dismutation than the untransformed line. Thus, ectopic expression of AnAFP gene improved chilling tolerance in the transgenic lines, which could be used to apply for further safety assessment for commercial breeding. | Yuanyuan Zhang Yang Cao Hongying Zheng Wenqi Feng Jingtao Qu Fengling Fu Wanchen Li Haoqiang Yu | 2021 | The Crop Journal2021，9，4： | 2 |
| 2 | Tunnel vision optimization method for VR flood scenes based on Gaussian blur显示文摘The visualization of flood disasters in virtual reality(VR)scenes is useful for the representation and sharing of disaster knowledge and can effectively improve users’cognitive efficiency in comprehending disaster information.However,the existing VR methods of visualizing flood disaster scenes have some shortcomings,such as low rendering efficiency and poor user experience.In this paper,a tunnel vision optimization method for VR flood scenes based on Gaussian blur is proposed.The key techniques are studied,such as region of interest(ROI)calculation and tunnel vision optimization considering the characteristics of the human visual system.A prototype system has been developed and used to carry out an experimental case analysis.The experimental results show that the number of triangles drawn in a flood VR scene is reduced by approximately 30%–40%using this method and that the average frame rate is stable at approximately 90 frames per second(fps),significantly improving the efficiency of scene rendering and reducing motion sickness. | Lin Fu Jun Zhu Weilian Li Qing Zhu Bingli Xu Yakun Xie Yunhao Zhang Ya Hu Jingtao Lu Pei Dang Jigang You | 2021 | International Journal of Digital Earth2021，14，7： | 1 |
| 3 | Sponge-based materials for oil spill cleanups: A review显示文摘Elimination of leaked oil from aquatic environs has recently gained importance owing to the disasters associated with leakages into marine environments.The need for an environmentally friendly and viable line of action concerning the environs has brought forward numerous affordable,non-toxic,and decomposable materials;further,diverse biomasses for fabricating nano-to micro-scale materials,membranes,and sponges/aerogels have also been incorporated for the elimination and retrieval of oils from water.Moreover,selectivity,sorption capacity,and reusability of these materials after the retrieval of oils are also desired from the viewpoint of sustainability.This review encompasses the recent progress in the field of elimination and retrieval of oil spills using various sponge-based materials. | Edward Mohamed Hadji Bo Fu Ayob Abebe Hafiz Muhammad Bilal Jingtao Wang | 2020 | Frontiers of Chemical Science and Engineering2020，14，5： | 1 |
| 4 | Effect of Different Thawing Methods on Post-thaw Motility of Cow Semen in Cryopreservation Straws显示文摘The study was to investigate the effects of different thawing temperatures(5,15,40,75,90 ℃) and times(1- 120 s) on properties of post-thaw cow semen by detecting frozen-thawed semen motility,acrosome integrity and tail membrane integrity,further obtaining the optimal thawing method of straw frozen semen from dairy cow. The results showed that(1) Thawing of the straw frozen semen of dairy cow at 75 ℃ for 3 s yielded the highest semen motility,followed by 40 ℃for 20 s,and the least by low temperature 5 ℃ and room temperature 15 ℃ with a semen motility of 0. 3,moreover thawing at high temperature 90 ℃ was not suitable for large scale production due to the difficult control of the temperature;(2) The acrosome intact rate and plasma membrane integrity rate of semens thawed at90 ℃ were remarkably lower than that at 40 ℃ and 75 ℃ significantly(P < 0. 05),while its semen malformation rate was significantly higher than that at 40 ℃and 75 ℃(P < 0. 05);(3) The Survival time of semens at 37 ℃ varied largely among different thawing temperature,in detail by 40 ℃ > 75 ℃ > 90 ℃. In practice,the thawing method of straw frozen semen of dairy cow should be selected according to the specific circumstance and inseminated immediately,with the recommended condition of thawing at 75 ℃ for 3 s. If the thawed semen could not be inseminated immediately,the thawing should be performed at 20 s for 40 ℃to maintain the motility for a longer term. | Qiao Limin Guo Tong Qiao Fuqiang Yao Hua Xiao Xishan Hou Yinxu Fu Jingtao Ren Kang | 2014 | Animal Husbandry and Feed Science2014，6，4： | 1 |
| 5 | Enhancement by IL-18 of the protective effect of a Schistosoma japonicum 26<ce:hsp sp='0.25'/>kDa GST plasmid DNA vaccine in mice显示文摘 | Feng Wei Quan Liu Shengyan Gao Limin Shang Yujia Zhai Jingtao Men Li Jiang Xing-Quan Zhu Zhiqiang Fu Yaojun Shi Zhiping Xia Jiaojiao Lin | 2008 | Vaccine2008，，33： | 1 |
| 6 | Effect of Calcium Treatment During Production on Quality of Stored Huangguan Pear Fruit显示文摘The effect of calcium treatment during production on the quality(soluble solids, fruit firmness, soluble sugar content, titratable acid content, etc.) of Huangguan pear was studied. The results showed that the calcium treatment during production improved the comprehensive quality of Huangguan pear fruit, delayed and reduced the occurrence of browning of fruit core, and enhanced its storage effect in the cold storage. Therefore, the calcium treatment improved the quality of stored pear fruit. | Tiezheng LIU Xueying LI yong LI Guosheng LIU Jingtao WANG Yongbo WANG Yali FU Yanxiao HAN | 2019 | Agricultural Biotechnology2019，8，2： | 0 |
| 7 | Effect of Postharvest Heat Treatment on Pear Browning during Storage显示文摘The effect of postharvest heat treatment on browning of Huangguan pear was investigated. The results showed that heat treatment improved hardness and soluble solid content of stored pear fruit,postponed the improvement of MDA content and PPO activity in stored pear fruit and alleviated the reduction of SOD activity and the lowering of PG activity. To sum up,heat treatment reduces the occurrence of browning of stored pear fruit through the regulation of the changes in a series of physiological and biochemical indexes of fruit. | Tiezheng LIU Jingtao WANG Xiangmin SUO Yali FU Wenzhang JI | 2018 | Agricultural Biotechnology2018，7，1： | 0 |
| 8 | Impacts of Exogenous Calcium and Calcium Inhibitor on Fruit Cracking of Apricot显示文摘Calcium nitrate and EGTA( calcium inhibitor) were sprayed on Dafeng Apricot during young fruit period. The results showed that calcium( Ca) spraying during young fruit period increased Ca and VCcontents in the fruit,improved SOD and POD activities,and declined K content,K/Ca and the occurrence of fruit cracking. When spraying calcium inhibitor during young fruit period,it aggravated fruit cracking. | Tiezheng LIU Jingtao WANG Xuehua LI Yali FU Xiangmin SUO | 2018 | Agricultural Biotechnology2018，7，3： | 0 |
| 9 | Effects of Storage Temperature on the Effective Activity of Straw Frozen Semen after Thawing显示文摘The aim was to discuss the optimal storage environment and proper insemination time after thawing of 0. 25 m L straw frozen semen. Straw frozen semen was thawed at 40 ℃ for 20 s,and then stored at 0- 4 ℃,14- 16 ℃,25- 27 ℃ for 2,4,6,8 and 10 h,respectively. The sperm motility was detected. After thawing,semen was stored at 0- 4 ℃ and 14- 16 ℃ for 10 h. Their sperm motilities( 0. 434 ± 0. 016 7 and 0. 423 ± 0. 019 6) had no significant differences( P> 0. 05) with initial thawing motility( 0. 441 ± 0. 030). Sperm motility reduced as the storage time prolonged at 25- 27 ℃. Sperm motility after 6 h had significant differences with that of initial thawing motility( P < 0. 05),and sperm motilities after 8 and 10 h showed extremely significant differences( P < 0. 01).Thus,sperm motility after thawing was still very high after stored at 0- 4 ℃ and 14- 16 ℃ within 10 h,which met the requirements for insemination. Under this temperature and time ranges,sperm could be carried over long distances,which had small effects on sperm quality and reached the expected insemination effects.However,under the temperature of 25- 27 ℃,semen should be used for insemination within 6 h after thawing. | Qiao Limin Ma Jianmin Qiao Fuqiang Yao Hua Xiao Xishan Hou Yinxu Fu Jingtao Ren Kang | 2015 | Animal Husbandry and Feed Science2015，7，4： | 0 |
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