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| 1 | Gambogic acid induces mitochondria-dependent apoptosis by modulation of Bcl-2 and Bax in mantle cell lymphoma JeKo-1 cells显示文摘Aim: To study the mechanisms in gambogic acid (GA) -induced JeKo-1 human Mantle Cell Lymphoma cell apoptosis in vitro. Methods: The proliferation of GA-treated JeKo-1 cells was measured by CCK-8 assay and Ki-67 immunocytochemical detection. Apoptosis, cell cycle and mitochondrial membrane potential were measured by flow cytometric analysis. Caspase-3,-8 and -9 were detected by colorimetric assay. Bcl-2 and Bax were analyzed by Western blotting. Results: GA inhibited cell growth in a time-and dose-dependent manner. GA induces apoptosis in JeKo-1 cells but not in normal bone marrow cells, which was involved in reducing the membrane potential of mitochondria, activating caspases-3, -8 and -9 and decreasing the ratio of Bcl-2 and Bax without cell cycle arresting. Conclusions: GA induced apoptosis in human MCL JeKo-1 cells by regulating Bcl-2/Bax and activating caspase-3, -8 and -9 via mitochondrial pathway without affecting cell cycle. | Jingyan Xu Min Zhou Jian Ouyang Jing Wang Qiguo Zhang Yong Xu Yueyi Xu Qian Zhang Xihui Xu Hui Zeng | 2013 | Chinese Journal of Cancer Research2013，25，2： | 18 |
| 2 | Leaching and Redistribution of Nutrients in Surface Layer of Red Soils in Southeast China显示文摘The leaching and redistribution of nutrients in the surface layer of 4 types of red soils in Southeast China were studied with a lysimeter experiment under filed conditions.Results showed that the leaching concentrated in the rainy season(from April to June).Generally,the leaching of soil nutrients from the surface layer of red soils was in the order of Ca>Mg>K>NO3-N,In fertilization treatment,the total amount of soil nutrients leached out of the surface layer in a red soil derived from granite was the highest in all soils.The uptake by grass decreased the leaching of fertilizer ions in surface layer,particularly for No3-N,Soil total N and exchangeable K,Ca and Mg in the surface layer decreased with leaching and grass uptake during the 2 years without new fertilization of urea,Ca(H2PO4)2,KCl,CaCO3 and MgCO3,Ca moved from the application layer(0-5cm) of fertilizer and accumulated in the 10-30cm depth in the soils studied except that derved from quaternary red clay.The deficiency of soil exchangeable K will become a serious degradation process facing the Southeast China. | SUN BO ZHANG TAOLIN and ZHAO QIGUO(Institute of Soil Science, the Chinese Academy of Sciences, P.O. Box 821, Naniing 210008 China) | 2000 | Pedosphere2000，10，2： | 14 |
| 3 | Predicting As,Cd and Pb uptake by rice and vegetables using field data from China显示文摘Plant uptake factor(PUF),single-variable regression of natural log-transformed concentrations in rice grain/vegetables versus natural log-transformed concentrations in soil and multiple-variable regression with soil concentrations and pH,was derived,validated and compared based on the paired crop and soil data collected from studies regarding As,Cd and Pb contaminated croplands in China.Results showed that the median value of PUF did not present deterministic prediction.But after natural logarithm transformation,the PUF followed Gaussian distribution which could be useful in risk assessment.The single-variable regression models were significant for As,Cd and Pb uptake both by rice and vegetables;however,the standard errors of all the regressions were comparatively large.Soil pH as a variable was generally significant but it only contributed positively to model fit for Cd uptake.After model comparison and selection,the upper 95% prediction limits of the multiple regression model for Cd uptake by rice was recommended to calculate screening value of Cd for paddy soil based on the limit for Cd concentration in rice grain. | Hongzhen Zhang Yongming Luo Jing Song Haibo Zhang Jiaqi Xia Qiguo Zhao | 2011 | Journal of Environmental Sciences2011，23，1： | 12 |
| 4 | Control mechanism for the upper airway collapse in patients with obstructive sleep apnea syndrome: a finite element study显示文摘Obstructive sleep apnea syndrome (OSAS) is characterized by recurrent collapses of the upper airway, which lead to repetitive transient hypoxia, arousals and finally sleep fragmentation. Both anatomical and neuromuscular factors may play key roles in the pathophysiology of OSAS. The purpose of this paper was to study the control mechanism of OSAS from the mechanical point of view. A three-dimensional finite element model was developed, which not only reconstructed the realistic anatomical structure of the human upper airway, but also included surrounding structures such as the skull, neck, hyoid, cartilage and soft tissues. The respiration process during the normal and apnea states was simulated with the fluid-structure interaction method (FSI) and the computational fluid dynamics method (CFD). The airflow and deformation of the upper airway obtained from the FSI and the CFD method were compared and the results obtained under large negative pressure during an apnea episode were analyzed. The simulation results show that the FSI method is more feasible and effective than the CFD method. The concave configuration of the upper airway may accelerate the collapse of the upper airway in a positive feedback mechanism, which supplies meaningful information for clinical treatment and further research of OSAS. | HUANG RenHan LI XiPing RONG QiGuo | 2013 | Science China(Life Sciences)2013，56，4： | 12 |
| 5 | Resource and Environmental Quality Changes and Adjustment Principles for Sustainable Development in Rapidly Developing Coastal Region of Southeastern China显示文摘Problems in water-land resources and environmental quality increase with the fast economic development in the coastal region of southeastern China. This region has the highest density in population, industry and towns in China, and the large export-oriented economy makes itself a strategic importance while China enters into international markets. The problems facing to this region include arable land reduction, land degradation aggravation, high percentage of built-up land with high rate of expansion, expanded non-point pollution, deteriorated water quality, decreasing biodiversity, destroyed ecosystems, severe air pollution, frequently occurred acid rain, and multi-pollutants. The important research fields in the near future should include a) influence of high-intensity exploitation of resources on changes of environment quality and its feedback; b) interface processes, key mechanisms and adjustment principles for degradation of water-, land-, and air-resources and environmental quality; c) evolvement processes and nurturing theory of biodiversity and ecological resources; and d) spatio-temporal variation and human-induced effects on regional resources and environment quality. Strategies for sustainable development in the region are as follows: a) controlling to ensure basic cultivated land area; b) enforcing remediation of polluted water environment, and controlling non-point pollution; c) strengthening ecological construction and ecological security; and d) controlling multi-pollution and preventing trace toxic pollutants. | ZHAO QIGUO Institute of Soil Science, the Chinese Academy of Sciences, P. O. Box 821, Nanjing 210008 (China) | 2001 | Pedosphere2001，11，4： | 9 |
| 6 | Evaluation of the Customer Satisfaction Index for Wellhead Blowout Preventers of China’s Petroleum Industry——A new method based on PLS显示文摘This paper establishes an evaluation model of the customer satisfaction index for the wellhead blowout preventers of China’s petroleum industry based on evaluation models of the customer satisfaction index at home and aboard, and by considering the consuming situation in China and the features of the China’s petroleum industry. For the existence of: (1) multiple correlations among the factors in the model; (2) the variables need to be explained, but that are hard to observe; (3) the customer satisfaction degree of observation variables appears the shape of skewness or two or three peaks, the correlations between the satisfaction index and its factors cannot be described by common multiple regression. This paper uses a partial least squares (PLS) method based on principal components and typical correlative analysis to solve the problem. When PLS is used in the model of the customer satisfaction index of the wellhead blowout preventers, the latent variables and the explanation degree coefficient of the manifest variable to the corresponding latent variables are estimated by PLS path analysis, and the influencing coefficient among the latent variables in the model is estimated by PLS regression analysis. PLS is also be used to calculate and analyze the model and disclose the correlations among the structural variables as well as the correlation between structural variables and its corresponding observation variables, evaluating results of which provide useful information for petroleum industry to improve the product quality and to the enhancement of the customer satisfaction to the product. | Zhang Peng Hu Qiguo Han Xia Zhang Bin | 2005 | Petroleum Science2005，2，3： | 8 |
| 7 | Nitrogen Mineralization of Prunings of Six N_2-Fixing Hedgerow Species in a Dry Valley of the Jinsha River显示文摘A litterbag experiment of 12 weeks was conducted to study nitrogen mineralization process of prunings of six nitrogen-fixing hedgerow species in a dry valley of the Jinsha River. Prunings were incorporated into soil or used as mulch. The results indicated that pruning N of the six hedgerow species was mineralized fast in the first week and then decreased slowly in the rest of the study period. When prunings were incorporated into soil, the amount of nitrogen mineralized by the end of the first week accounted for 69.9%, 58.2%, 54.5%, 43.0%, 29.6% and 20.6% of the total N in prunings of Desmodium rensonii, Tephrosia Candida, Leucaena leucocephala, Albizia yunnanensis, Acacia dealbata, and Acacia mearnsii, respectively. When prunings of L. leucocephala were used as mulch materials, the amount of nitrogen mineralized in the first week was 16.2% less than that of prunings incorporated into soil. The mineralization pattern of pruning N could be simulated by an exponent model Nt% = N01% (1 ?exp(梜1t))+ No2% (1 ?exp(梜2t) where Nt% is cumulative mineralized N in time t, N01 % and No2 % are readily and less readily mineralizable N in prunings, respectively, and k1 and k2 are rate constants. A half-life period of pruning nitrogen mineralization could also be determined by this model. The nitrogen content in the pruning residues decreased quickly in the first week but fluctuated thereafter. The initial C/N ratio was negatively related to the mineralization rate of prunings. | SUN HUI, TANG YA and ZHAO QIGUO Institute of Soil Science, the Chinese Academy of Sciences, Nanjing, 210008 (China) Chengdu Institute of Biology, the Chinese Academy of Sciences, Chengdu 610041 (China) | 2002 | Pedosphere2002，12，1： | 7 |
| 8 | Pedosphere and Its Effect on Global Changes显示文摘With development of modern geoscience, particularly development of environmental sciences, the contemporary soil science is undergoing great changes in both research contents and scope. Soil is not onlya certain substance or a certain independent natural historical body but also a spheric layer with peculiarstructure and functions in the earth system. From the viewpoint of the geo-biosphere system of earth, soilscience does deal not only with the soil substances per se but also more importantly with the relationshipsamong soil, the other spheres and the human survival environment in view of the 'pedosphere'. This is thenew orientation of soil science today and will affect profoundly the studies on the human survival environmentand global changes. To throw more light on this subject, the present paper intends to address the conceptionof pedosphere and its role in global changes. Also addressed are series of environmental issues in China andtheir relations to the global changes. Moreover, research orientation and priorities are indicated, includingexploitation and protection of the soil resources, soil fertility and sustainable agricultural development, construction of the ecological environment, and the material cycling in pedosphere and its relation to globalchanges. | ZHAO QIGUO and CAO HUI(Institute of Soil Science, the Chinese Academy of Sciences, P.O. Box 821, Nauling 210008 China) | 2000 | Pedosphere2000，10，2： | 5 |
| 9 | Biomechanics of pelvic organ prolapse显示文摘Dear Editor,Pelvic organ prolapse(POP),caused by damage of the support system in the pelvic floor,is downward descent of female pelvic organs,resulting in protrusion of the vagina,uterus,or both.It has been reported recently that POP has a prevalence of 3%?6%when defined by symptoms and up to50%based on a vaginal examination[1].POP is | REN Shuang XIE Bing WANG JianLiu RONG QiGuo | 2015 | Science China(Life Sciences)2015，58，2： | 5 |
| 10 | A new strain of Crimean-Congo hemorrhagic fever virus isolated from Xinjiang, China显示文摘Crimean-Congo hemorrhagic fever virus(CCHFV) is a highly pathogenic tick-borne virus with a fatality rate of up to 50% in humans. CCHFV is widely distributed in countries around the world.Outbreaks of CCHFV infection in humans have occurred in prior years in Xinjiang Province, China.Epidemiological surveys have detected CCHFV RNA in ticks and animals; however, few isolates were identified. In this study, we identified and isolated a new CCHFV strain from Hyalomma asiaticum asiaticum ticks collected from north of Tarim Basin in Xinjiang, China. A preliminary investigation of infection and antigens expression of CCHFV was performed in newborn mice. The target tissues for CCHFV replication in newborn mice were identified. The analysis of the phylogenetic relationships with other Chinese strains suggested that diverse genotypes of CCHFV have circulated in Xinjiang for years. These findings provide important insights into our understanding of CCHFV infection and evolution as well as disease prevention and control for local residents. | Rong Guo Shu Shen Yanfang Zhang Junming Shi Zhengyuan Su Dan Liu Jinliang Liu Juan Yang Qiguo Wang Zhihong Hu Yujiang Zhang Fei Deng | 2017 | Virologica Sinica2017，32，1： | 5 |
| 11 | Leaching of Nutrient Elements in a Red Soil Derived from Quaternary Red Clay显示文摘A red soil derived from Quaternary red clay was employed to study nutrient leaching with soil columns repacked in laboratory. The objective was to identify the effects of fertilization practices on leaching patterns and magnitudes of Ca2+, Mg2+, K+, NH+, and NO-. The treatments were CK (as a control), CaCO3,CaSO4, MgCO3, Ca(H2PO4)2, urea, KCI, and multiple (a mixture of the above-mentioned fertilizers). The fertilizers were added to the bare surface of the soil columns, and then the columns were leached with 120 mL deionized water daily through peristaltic pumps over a period of 92 days. Leaching processes of NH+, and NO- were only measured in CK, urea, and multiple treatments which were directly related to N leaching.Results showed that sole application of CaSO4, and Ca(H2PO4)2 scarcely had any effect on the leaching losses of Ca2+, Mg2+, and K+; the application of MgCO3 stimulated the leaching of Mg2+; the application of CaCOs promoted the leaching of Ca2+, Mg2+ and K+; urea treatment also promoted the leaching of K+ and NH+, and NO- leaching mainly occurred at late stage of leaching process in particular; under KCI treatment, leaching of Ca2+, Mg2+, and K+ was promoted to a large extent; under multiple treatment,leaching of Ca2+, Mg2+, K+, NH+, and NO- was all increased and NO- was mainly leached at the end of leaching process and still had a trend of increase. | SHEN RENFANG and ZHAO QIGUO(Laboratory Of Material Cycling in Pedosphere, Institute of Soil Science, the Chinese Academy of Sciences,P.O. Box 821, Nanjing 210008 (China)) | 1998 | Pedosphere1998，8，1： | 5 |
| 12 | An efficient algorithm to compute transient pressure responses of slanted wells with arbitrary inclination in reservoirs显示文摘Compared with vertical and horizontal wells, the solution and computation of transient pressure responses of slanted wells are more complex. Vertical and horizontal wells are both simplified cases of slanted wells at particular inclination, so the model for slanted wells is more general and more complex than other models for vertical and horizontal wells. Many authors have studied unsteady-state flow of fluids in slanted wells and various solutions have been proposed. However, until now, few of the published results pertain to the computational efficiency. Whether in the time domain or in the Laplace domain, the computation of integration of complex functions is necessary in obtaining pressure responses of slanted wells, while the computation of the integration is complex and time-consuming. To obtain a perfect type curve the computation time is unacceptable even with an aid of high-speed computers. The purpose of this paper is to present an efficient algorithm to compute transient pressure distributions caused by slanted wells in reservoirs. Based on rigorous derivation, the transient pressure solution for slanted wells of any inclination angle is presented. Assuming an infinite-conductivity wellbore, the location of the equivalent-pressure point is determined. More importantly, according to the characteristics of the integrand in a transient pressure solution for slanted wells, the whole integral interval is partitioned into several small integral intervals, and then the method of variable substitution and the variable step-size piecewise numerical integration are employed. The amount of computation is significantly reduced and the computational efficiency is greatly improved. The algorithm proposed in this paper thoroughly solved the difficulty in the efficient and high-speed computation of transient pressure distribution of slanted wells with any inclination angle. | Wang Haitao Zhang Liehui Guo Jingjing Liu Qiguo He Xinming | 2012 | Petroleum Science2012，9，2： | 3 |
| 13 | Spatial Distribution and Changes of Heavy Metals of Agricultural Lands in Typical Pregrading Coast in Dongtai City,Jiangsu Province,China显示文摘According to the historical changes of coastal lines, seven soil sampling districts, from land to sea, were arranged in Dongtai City, Jiangsu Province to sample soils from surface and profile. Concentrations of seven major heavy metals (HMs), granularity, pH, organic matters and C/N of the soil samples were analyzed. Results show that concentrations of heavy metals in agricultural land present a certain spatial variance, decreasing from land to sea. Pollution assessment indicates that the agricultural soils were not polluted by HMs, but the potential pollution of Cu and Hg needs to be alerted. Different HMs accumulate in the surface and sub-surface of the soil profiles, and concentrations of Hg and Pb decrease significantly with the increment of soil depth. Concentrations of HMs exhibit a significantly negative correlation to pH, but have no significant relation with organic matters in soil. Principle component analysis show that the concentrations of HMs relate to the land use history. Concentrations of Hg, Ni and Cr in soil are closely related with land use history, and concentrations of Pb, Cu and Cr are affected by land use history as well as other factors. However, there is no significant relation between concentration of As and land use history. | LIAO Fuqiang ZHOU Shenglu ZHANG Hongfu WU Shaohua ZHAO Qiguo | 2008 | Chinese Geographical Science2008，18，3： | 3 |
| 14 | A new method of measuring optical turbulence of atmospheric surface layer at Antarctic Taishan Station with ultrasonic anemometer显示文摘To find the optimal location for large-aperture telescopes is a goal of astronomy. Chinese Antarctic astronomy has begun to flourish in recent years, and it is an urgent need in basic astronomical work to measure and analyze the optical turbulence spatiotemporal distribution in the Antarctic region. We analyzed turbulence data measured by a mobile atmospheric parameter measurement system from 30 December 2013 to 10 February 2014 at Antarctic Taishan Station. Because there is a discrepancy between the refractive index structure constant C_n^2 measured by an ultrasonic anemometer with a single-point temperature structure function method and by micro-thermometer, a new method to measure C_n^2 with a temperature spectrum method is proposed herein. Through comparing long-term continuous C_n^2 data derived from ultrasonic anemometer with those via the new method and micro-thermometer, trend, magnitude and measured weak turbulence of ~2×10^(-16)m^(-2/3) are generally satisfactory. The reason for the discrepancy in C_n^2 measurement between the ultrasonic anemometer with the old method and micro-thermometer is investigated. | WU Xiaoqing TIAN Qiguo JIANG Peng CHAI Bo QING Chun CAI Jun JIN Xinmiao ZHOU Hongyan | 2015 | Advances in Polar Science2015，26，4： | 3 |
| 15 | N-terminal domains of ARC1 are essential for interaction with the N-terminal region of Exo70A1 in transducing self-incompatibility of Brassica oleracea显示文摘自我障碍(SI ) 是一个重要交配系统阻止使近交并且支持 outcrossing。ARC1 和 Exo70A1 在 Brassica SI 发信号作为 S 地点受体 kinase 和戏保守人士角色的下游的目标工作。基于顺序相同, Exo70A1 被划分成四子域:白氨酸拉链(列伊 128-Leu149), hypervariable 区域(重量的单位 172-Leu197), 相扑修正主题(Glu 260-Ile275), 和 pfamExo70 领域(他的 271-Phe627) 。ARC1 如下包含四个领域:白氨酸拉链(列伊 116-Leu137), 卷卷领域(Thr 210-Val236),U 盒子(毒蛇 282-Trp347) 主题,和手臂(翼 415-Thr611) 领域。生物信息学分析,酵母二混血儿的屏蔽和 ARC1 116-236 的白氨酸拉链和卷卷主题为和 Exo70A1 的相互作用被要求的下拉试金表演,当手臂主题的增加导致和 Exo70A1 的相互作用的损失时。同时,没有任何域的 Exo70A1 的N终端与 ARC1 显示出一个弱相互作用,并且 LacZ 表示的水平与白氨酸拉链的增加增加并且与 hypervariable 区域和相扑修正主题到达最大的值,显示那个 hypervariable 区域和 Exo70A1 172-275的相扑修正主题为有 ARC1 的绑定主要负责,而 pfamExo70 领域为 ARC1 有很少亲密关系。位于 Exo70A1 hypervariable 区域的 Lys 181 可以是调停的 ubiquitination 地点在 ARC1 和 Exo70A1 之间的相互作用。因此,两有 ARC1 116-236, 和 hypervariable 区域和 Exo70A1 172-275 的相扑修正主题的卷卷结构的白氨酸拉链是在 ARC1 和 Exo70A1 之间的核心相互作用领域。影响这些核心领域的任何因素将是调停的 ARC1 的管理者在自我不兼容的系统的 ubiquitin 降级。 | Songmei Shi Qiguo Gao Jing Zeng Xiaohuan Liu Quanming Pu Guixi Liu Hecui Zhang Xiaohong Yang Liquan Zhu | 2016 | Acta Biochimica et Biophysica Sinica2016，48，9： | 3 |
| 16 | K-vertex-connectivity minimum augmentation for undirected unweighted graphs显示文摘For an undirected unweighted graph G0=(V0,E0) and a positive integer K, the K-vertex-connectivity minimum augmentation problem (K-VCMAP) is to find a minimum set of edges Emin such that the graph H0=(V0,E0∪Emin) is K-vertex-connected. Results in the literature have given polynomial time algorithms for K-VCMAP in several special cases such as where k≤3, or G0 is a tree. However, it still remains open whether or not there exist polynomial time algorithms for K-VCMAP for any graph G0 and any integer K. In this paper, we settle the problem by describing an efficient algorithm (KUCA) with time-complexity of O(K|V(G0)|5) for the K-VCMAP for any G0 and any positive integer K. | CAO Qiguo, WU Xue and SUN Yugeng(School of Electrical Automation and Energy Engineering, Tianjin University, Tianjin 300072, China) | 2003 | Progress in Natural Science:Materials International2003，13，6： | 3 |
| 17 | A mobile polar atmospheric parameter measurement system:Ⅱ. First atmospheric turbulence observation at Antarctic Taishan Station显示文摘This is the second paper of a series devoted to atmospheric optical turbulence C2 n observation using a mobile polar atmospheric parameter measurement system. We present the initial results of C2 n measurement at Antarctic Taishan Station using micro-thermal sensors and a three-dimensional sonic anemometer at height ~2.0 m above the snow surface. The site testing experiments were carried out during the 30 th Chinese National Antarctic Research Expedition(CHINARE). We collected about 1 000 h of data between 30 December 2013 and 10 February 2014. The C2 n curve exhibits clear daily structures, with two peaks around midnight and midday and two troughs around 7:30 and 17:00 local time(UTC+5). The mean C2 n is 2.7×10-15 m-2/3 and the 25 th and 75 th percentiles of the C215 n cumulative distribution are 9.6×10-16 m-2/3 and 6.2×10- m-2/3, respectively. Meteorological parameters such as temperature, relative humidity, wind speed, and air pressure are also presented. | TIAN Qiguo JIANG Peng WU Xiaoqing JIN Xinmiao LU Shan JI Tuo CHAI Bo ZHANG Shaohua ZHOU Hongyan | 2015 | Advances in Polar Science2015，26，2： | 2 |
| 18 | The Effect of Online Channel Addition on Store Performance:Empirical Evidence from Chinese Chain Retailers显示文摘With the rapid development of e-Commerce and takeaway platforms, retailers have gradually developed multi-channel operations. However, limited empirical studies explored the effects of an online channel offered by takeaway platforms on the store performance. Does an online channel addition have a synergy effect or a cannibalization effect on store performance? We empirically investigate these effects by analyzing a large dataset including diverse samples collected from multiple retailers across various regions. The dataset includes 2115 stores across 25 retailers for 10 months that includes two types of retail formats and covers 16 provinces and 21 cities of China. We study the impacts of the newly introduced online channel on the incumbent offline channel and the overall store performance.The empirical results reveal that the online channel addition mainly has a synergy effect. Specifically,for the overall store, it has a positive effect on the sales and product variety, whereas it has a negative effect on the basket size. Surprisingly, an online channel addition also has a positive effect on the offline sales. Our study adds novel values to multi-channel retailing literature by empirically researching the cannibalization and synergy effect of a new type of online channel, offered by takeaway platforms. It can provide insights for retail enterprises who are interested in introducing O2 O model. | Yixin WANG Qiguo GONG Tingyan WANG Xin TIAN | 2021 | Journal of Systems Science and Information2021，9，4： | 2 |
| 19 | Identification and characterization of BoPUB3: a novel interaction protein with S-locus receptor kinase in Brassica oleracea L.显示文摘Armadillo repeat containing 1 (ARC1) is phosphorylated by S-locus receptor kinase (SRK) and functions as a positive regulator in self-incompatibility response of Brassica. However, ARC1 only causes partial breakdown of the self-incompatibility response, and other SRK downstream factors may also participate in the self-incompatibility signaling pathway. In the present study, to search for SRK downstream targets, a plant U-box protein 3 (BoPUB3) was identified from the stigma of Brassica oleracea L. BoPUB3 was highly expressed in the stigma, and its expression was increased with the stigma development and reached to the highest level in the mature-stage stigma. BoPUB3, a 76.8-kDa protein with 697 amino acids, is a member of the PUB-ARM family and contains three domain characteristics of BoARC1, including a U-box N-terminal domain, a U-box motif, and a C-terminal arm repeat domain. The phylogenic tree showed that BoPUB3 was close to BoARC1. The synteny analysis revealed that B. oleracea chromosomal region containing BoPUB3 had high synteny with the Arabidopsis thaliana chromosomal region containing AtPUB3 (At3G54790). In addition, the subcellular localization analysis showed that BoPUB3 primarily localized in the plasma membrane and also in the cytoplasm. The combination of the yeast two-hybrid and in vitro binding assay showed that both BoPUB3 and BoARC1 could interact with SRK kinase domain, and SRK showed much higher level of β-galactosidase activity in its interaction with BoPUB3 than with BoARC1. These results implied that BoPUB3 is a novel interactor with SRK, which lays a basis for further research on whether PUB3 participates in the self-incompatibility signaling pathway. | Songmei Shi Qiguo Gao Tonghong Zuo Zhenze Lei Quanming Pu Yukui Wang Guixi Liu Xinhua He Xuesong Ren Liquan Zhu | 2019 | Acta Biochimica et Biophysica Sinica2019，51，7： | 2 |
| 20 | Review of Researches on Advantages of Intercropping显示文摘Intercropping is a very effective cultivation method in the agricultural production with a long history. The use of intercropping to build a niche complementary crop community can not only enhance the stability of farmland ecosystem and the resistance of crop community to environmental stress but also reduce the damage of diseases, insect pests, environmental pollution and improve the efficiency of resource use to finally achieve high and stable yield of crop community. By collecting the intercropping researches at home and abroad, this thesis describes the impact of intercropping on crop nitrogen fixation and nitrogen transfer, accumulation and distribution of soil nitrate nitrogen, soil microorganism, crop diseases and insect pests, ventilation and light transmission, quality, yield and nutrient absorption, draws a conclusion on intercropping advantages and points out the sections requiring further study in the future. | ZHANG Xiang-qian HUANG Guo-qin BIAN Xin-min JIANG Xue-hai ZHAO Qiguo | 2012 | Asian Agricultural Research2012，4，12： | 2 |
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