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| 1 | Gambogic acid induces mitochondria-dependent apoptosis by modulation of Bcl-2 and Bax in mantle cell lymphoma JeKo-1 cells显示文摘Aim: To study the mechanisms in gambogic acid (GA) -induced JeKo-1 human Mantle Cell Lymphoma cell apoptosis in vitro. Methods: The proliferation of GA-treated JeKo-1 cells was measured by CCK-8 assay and Ki-67 immunocytochemical detection. Apoptosis, cell cycle and mitochondrial membrane potential were measured by flow cytometric analysis. Caspase-3,-8 and -9 were detected by colorimetric assay. Bcl-2 and Bax were analyzed by Western blotting. Results: GA inhibited cell growth in a time-and dose-dependent manner. GA induces apoptosis in JeKo-1 cells but not in normal bone marrow cells, which was involved in reducing the membrane potential of mitochondria, activating caspases-3, -8 and -9 and decreasing the ratio of Bcl-2 and Bax without cell cycle arresting. Conclusions: GA induced apoptosis in human MCL JeKo-1 cells by regulating Bcl-2/Bax and activating caspase-3, -8 and -9 via mitochondrial pathway without affecting cell cycle. | Jingyan Xu Min Zhou Jian Ouyang Jing Wang Qiguo Zhang Yong Xu Yueyi Xu Qian Zhang Xihui Xu Hui Zeng | 2013 | Chinese Journal of Cancer Research2013，25，2： | 18 |
| 2 | Biomechanics of pelvic organ prolapse显示文摘Dear Editor,Pelvic organ prolapse(POP),caused by damage of the support system in the pelvic floor,is downward descent of female pelvic organs,resulting in protrusion of the vagina,uterus,or both.It has been reported recently that POP has a prevalence of 3%?6%when defined by symptoms and up to50%based on a vaginal examination[1].POP is | REN Shuang XIE Bing WANG JianLiu RONG QiGuo | 2015 | Science China(Life Sciences)2015，58，2： | 5 |
| 3 | A new strain of Crimean-Congo hemorrhagic fever virus isolated from Xinjiang, China显示文摘Crimean-Congo hemorrhagic fever virus(CCHFV) is a highly pathogenic tick-borne virus with a fatality rate of up to 50% in humans. CCHFV is widely distributed in countries around the world.Outbreaks of CCHFV infection in humans have occurred in prior years in Xinjiang Province, China.Epidemiological surveys have detected CCHFV RNA in ticks and animals; however, few isolates were identified. In this study, we identified and isolated a new CCHFV strain from Hyalomma asiaticum asiaticum ticks collected from north of Tarim Basin in Xinjiang, China. A preliminary investigation of infection and antigens expression of CCHFV was performed in newborn mice. The target tissues for CCHFV replication in newborn mice were identified. The analysis of the phylogenetic relationships with other Chinese strains suggested that diverse genotypes of CCHFV have circulated in Xinjiang for years. These findings provide important insights into our understanding of CCHFV infection and evolution as well as disease prevention and control for local residents. | Rong Guo Shu Shen Yanfang Zhang Junming Shi Zhengyuan Su Dan Liu Jinliang Liu Juan Yang Qiguo Wang Zhihong Hu Yujiang Zhang Fei Deng | 2017 | Virologica Sinica2017，32，1： | 5 |
| 4 | An efficient algorithm to compute transient pressure responses of slanted wells with arbitrary inclination in reservoirs显示文摘Compared with vertical and horizontal wells, the solution and computation of transient pressure responses of slanted wells are more complex. Vertical and horizontal wells are both simplified cases of slanted wells at particular inclination, so the model for slanted wells is more general and more complex than other models for vertical and horizontal wells. Many authors have studied unsteady-state flow of fluids in slanted wells and various solutions have been proposed. However, until now, few of the published results pertain to the computational efficiency. Whether in the time domain or in the Laplace domain, the computation of integration of complex functions is necessary in obtaining pressure responses of slanted wells, while the computation of the integration is complex and time-consuming. To obtain a perfect type curve the computation time is unacceptable even with an aid of high-speed computers. The purpose of this paper is to present an efficient algorithm to compute transient pressure distributions caused by slanted wells in reservoirs. Based on rigorous derivation, the transient pressure solution for slanted wells of any inclination angle is presented. Assuming an infinite-conductivity wellbore, the location of the equivalent-pressure point is determined. More importantly, according to the characteristics of the integrand in a transient pressure solution for slanted wells, the whole integral interval is partitioned into several small integral intervals, and then the method of variable substitution and the variable step-size piecewise numerical integration are employed. The amount of computation is significantly reduced and the computational efficiency is greatly improved. The algorithm proposed in this paper thoroughly solved the difficulty in the efficient and high-speed computation of transient pressure distribution of slanted wells with any inclination angle. | Wang Haitao Zhang Liehui Guo Jingjing Liu Qiguo He Xinming | 2012 | Petroleum Science2012，9，2： | 3 |
| 5 | The Effect of Online Channel Addition on Store Performance:Empirical Evidence from Chinese Chain Retailers显示文摘With the rapid development of e-Commerce and takeaway platforms, retailers have gradually developed multi-channel operations. However, limited empirical studies explored the effects of an online channel offered by takeaway platforms on the store performance. Does an online channel addition have a synergy effect or a cannibalization effect on store performance? We empirically investigate these effects by analyzing a large dataset including diverse samples collected from multiple retailers across various regions. The dataset includes 2115 stores across 25 retailers for 10 months that includes two types of retail formats and covers 16 provinces and 21 cities of China. We study the impacts of the newly introduced online channel on the incumbent offline channel and the overall store performance.The empirical results reveal that the online channel addition mainly has a synergy effect. Specifically,for the overall store, it has a positive effect on the sales and product variety, whereas it has a negative effect on the basket size. Surprisingly, an online channel addition also has a positive effect on the offline sales. Our study adds novel values to multi-channel retailing literature by empirically researching the cannibalization and synergy effect of a new type of online channel, offered by takeaway platforms. It can provide insights for retail enterprises who are interested in introducing O2 O model. | Yixin WANG Qiguo GONG Tingyan WANG Xin TIAN | 2021 | Journal of Systems Science and Information2021，9，4： | 2 |
| 6 | Identification and characterization of BoPUB3: a novel interaction protein with S-locus receptor kinase in Brassica oleracea L.显示文摘Armadillo repeat containing 1 (ARC1) is phosphorylated by S-locus receptor kinase (SRK) and functions as a positive regulator in self-incompatibility response of Brassica. However, ARC1 only causes partial breakdown of the self-incompatibility response, and other SRK downstream factors may also participate in the self-incompatibility signaling pathway. In the present study, to search for SRK downstream targets, a plant U-box protein 3 (BoPUB3) was identified from the stigma of Brassica oleracea L. BoPUB3 was highly expressed in the stigma, and its expression was increased with the stigma development and reached to the highest level in the mature-stage stigma. BoPUB3, a 76.8-kDa protein with 697 amino acids, is a member of the PUB-ARM family and contains three domain characteristics of BoARC1, including a U-box N-terminal domain, a U-box motif, and a C-terminal arm repeat domain. The phylogenic tree showed that BoPUB3 was close to BoARC1. The synteny analysis revealed that B. oleracea chromosomal region containing BoPUB3 had high synteny with the Arabidopsis thaliana chromosomal region containing AtPUB3 (At3G54790). In addition, the subcellular localization analysis showed that BoPUB3 primarily localized in the plasma membrane and also in the cytoplasm. The combination of the yeast two-hybrid and in vitro binding assay showed that both BoPUB3 and BoARC1 could interact with SRK kinase domain, and SRK showed much higher level of β-galactosidase activity in its interaction with BoPUB3 than with BoARC1. These results implied that BoPUB3 is a novel interactor with SRK, which lays a basis for further research on whether PUB3 participates in the self-incompatibility signaling pathway. | Songmei Shi Qiguo Gao Tonghong Zuo Zhenze Lei Quanming Pu Yukui Wang Guixi Liu Xinhua He Xuesong Ren Liquan Zhu | 2019 | Acta Biochimica et Biophysica Sinica2019，51，7： | 2 |
| 7 | Supply chain networks: Closed Jackson network models and properties显示文摘 | Qiguo Gong K.K. Lai Shouyang Wang | 2008 | International Journal of Production Economics2008，，2： | 1 |
| 8 | Novd two - phase oxidative cress - coupling of the two - component molecular crystal of 2 - naphthol and 2 - naphthylamine 显示文摘 | Ding Kuiling Xu Qiguo Wang Yang | 1997 | Chemical Communications (Cambridge)1997，7，： | 1 |
| 9 | Study and practice of the controlling technique on heat-harm during the tunneling in Zhaolou mine显示文摘This paper proposes the cooling system type and cooling equipment type which are deep mine with high temperature during the construction,and presents auxiliary cooling measures making up duct temperature rise since compression and heat transfer temperature rise. The cooling system designed against Zhaolou mine's ground temperature and weather conditions,with its sprinkler room handling an average temperature difference up to 19.5~23.8 ℃,and the average enthalpy difference could reach 48.4~60.7 kJ/kg. At the same time,it gets a series of basic data used for mine construction during the cooling system design and equipment selection according to the measured results; using the analysis software Matlab,it obtains the change relations between the temperature of sprinkler room and fan export supply air temperature,wind casing temperature rise and fan export supply air temperature,working face temperature and supply air temperature,used for the mine cooling which has the similar conditions. | Liu Heqing Hao Xiaoli Wang Yongjie Dai Huadong Lin Qiguo | 2008 | Engineering Sciences2008，6，4： | 0 |
| 10 | Effects of Sweet Potato Rotation and Intercropping on the Microbial Community of Rhizosphere Soil显示文摘To reveal the response mechanism of soil microbial community in different planting systems of sweet potato,the effects of rotation and intercropping on microbial community structure and carbon source utilization capacities of sweet potato rhizosphere soil were studied by using phospholipid fatty acid(PLFA)and ecological board(BIOLOG ECO)through field positioning experiments.In this study,three treatments were sweet potato continuous cropping,sweet potato-wheat rotation,and sweet potato-corn intercropping.The main results showed that compared with the sweet potato continuous cropping treatment,sweet potato rotation and intercropping changed the PLFA biomass of soil microorganisms;the contents of bacteria increased by 21.82%and 38.77%,respectively(P<0.05);the contents of actinomycetes increased by 6.98%and 12.77%,and the biomass of Gram-positive bacteria increased by 28.60%and 63.44%,respectively;and the biomass of Gram-negative bacteria increased by 18.21%and 22.29%,and the fungal contents decreased by 16.60%and 13.03%,respectively.With the extension of culture time,the average well color development(AWCD value)of sweet potato-corn intercropping was significantly higher than other two treatments.The utilization capacities of carboxylic acid compounds,polymers,carbohydrates,amino acids,and amines in the sweet potato-corn intercropping treatment were significantly increased by 17.28%,14.67%,54.17%,36.62%,and 20.00%,respectively,compared with the sweet potato continuous cropping treatment.The results of the multivariate analysis(RDA)showed that the changes of soil microbial community structure and functional diversity were controlled by many factors,and the soil available potassium and total nitrogen were the main driving factors.However,sweet potato-wheat rotation and sweet potato-corn intercropping could optimize the soil microbial community structure and enhance the microbial functional diversity,and the effect of sweet potato-corn intercropping treatment was better. | Qiguo HU Yajun LIU Wenjing WANG Qi WANG Honggang WANG Fengli CHU | 2023 | Agricultural Biotechnology2023，12，2： | 0 |
| 11 | SD-RLK28 positively regulates pollen hydration on stigmas as a PCP-Bβ receptor in Arabidopsis thaliana显示文摘Pollen hydration on dry stigmas is strictly regulated by pollen–stigma interactions in Brassicaceae. Although several related molecular events have been described, the molecular mechanism underlying pollen hydration remains elusive. Multiple B-class pollen coat proteins(PCP-Bs) are involved in pollen hydration. Here, by analyzing the interactions of two PCP-Bs with three Arabidopsis thaliana stigmas strongly expressing S-domain receptor kinase(SD-RLK), we determined that SD-RLK28 directly interacts with PCP-Bβ. We investigated pollen hydration, pollen germination, pollen tube growth, and stigma receptivity in the sd-rlk28 and pcp-bβ mutants. PCP-Bβ acts in the pollen to regulate pollen hydration on stigmas. Loss of SD-RLK28 had no effect on pollen viability, and sd-rlk28 plants had normal life cycles without obvious defects. However,pollen hydration on sd-rlk28 stigmas was impaired and pollen tube growth in sd-rlk28 pistils was delayed. The defect in pollen hydration on sd-rlk28 stigmas was independent of changes in reactive oxygen species levels in stigmas. These results indicate that SD-RLK28 functions in the stigma as a PCP-Bβ receptor to positively regulate pollen hydration on dry stigmas. | Li Guo Ziya Huang Xingyu Chen Min Yang Miaomiao Yang Ziwei Liu Xuejie Han Xiangjie Ma Xiaoli Wang Qiguo Gao | 2023 | Journal of Integrative Plant Biology2023，65，10： | 0 |
| 12 | Construct Protein-Protein Interaction Network by Mining Domain-Domain Interactions显示文摘Domain-domain interactions are important clues to inferring protein-protein interactions. Although about 8 000 domain-domain interactions are discovered so far,they are just the tip of the iceberg. Because domains are conservative and commonplace in proteins,domain-domain interactions are discovered based on pairs of domains which significantly co-exist in proteins. Meanwhile,it is realized that:( 1) domain-domain interactions may exist within the same proteins or across different proteins;( 2) only the domain-domain interactions across different proteins can mediate interactions between proteins;( 3) domains have biases to interact with other domains. And then,a novel method is put forward to construct protein-protein interaction network by using domain-domain interactions. The method is validated by experiments and compared with the state- of-art methods in the field. The experimental results suggest that the method is reasonable and effectiveness on constructing Protein-protein interactions network. | Zhixia Teng Maozu Guo Xiaoyan Liu Jin Li Qiguo Dai Chunyu Wang | 2016 | Journal of Harbin Institute of Technology(New Series)2016，23，4： | 0 |
| 13 | The bright star survey telescope for the planetary transit survey in Antarctica显示文摘Transiting extrasolar planets(exoplanets),especially those orbiting bright stars, are desired for study of the diversity of planetary compositions, internal structures and atmospheres beyond our solar system. Dome A at Antarctica is a promising site for planetary transit surveys,where the continuous darkness and the large clear-sky fraction in the winter months greatly enhance the detection efficiency. The Chinese Small Telescope ARray and the Antarctic Survey Telescopes are the first facilities that have been operated at Dome A for use in exoplanet surveys. To increase the sky coverage, a low-temperature-resistant wide-field robotic telescope, named the bright star survey telescope(BSST), has been developed to join the ongoing planetary transit survey in Antarctica. The BSST has an aperture size of 300 mm and is equipped with a largeframe 4K×4K CCD camera to receive starlight from a 3.°4×3.°4 field of view. The BSST was operated at Lijiang observatory in April and May 2015 for a test run.Photometric precision of 3.5 mmag was achieved for stars with V~11 mag using 75 s exposures. The transiting events of two Jupiter-size exoplanets, HAT-P-3b and HATP-12 b, were observed on May 10 and May 20, 2015,respectively. | Qiguo Tian Peng Jiang Fujia Du Jian Wang Zhengyang Li Xiaoyan Li Zhiyong Zhang Haiping Lu Xiangyan Yuan Huigen Liu Hui Zhang Luming Sun Liang Chang Jianguo Wang Shaohua Zhang Tuo Ji Xiheng Shi Jie Chen Guangyu Zhang Minghao Jia Jiajing Liu Junyan Zhou Xiang Pan Shucheng Dong Fengxin Jiang Hongfei Zhang Jilin Zhou Lifan Wang Hongyan Zhou | 2016 | Science Bulletin2016，61，5： | 0 |
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