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| 1 | Effects of the recombinant toxin protein rLj-RGD3 in multidrug-resistant human breast carcinoma cells显示文摘我们以前报导了那 Lj-RGD3,新奇 RGD 毒素蛋白质,从 Lampetra 的口腔的腺被孤立装饰用的梨树。recombinant 蛋白质 rLj-RGD3 有反侵略并且在肿瘤房间(HeLa 房间) 和在 vitro 的 endothelial 房间(ECV304 房间) 的反粘合剂活动,并且禁止 v3, v5,和 1 调停 integrin 的粘附。在这研究,我们在药抵抗的 MCF-7/Adr 胸癌细胞线和药敏感的父母线 MCF-7 调查了 rLj-RGD3 的 bioactivity,并且发现 rLj-RGD3 禁止了两根细胞线的生长。生物功能研究表明 rLj-RGD3 能在 MCF-7/Adr 导致 apoptosis,它在药敏感的父母线 MCF-7 是比那更流行的。另外, rLj-RGD3 禁止了 MCF-7/Adr 房间的粘附到 fibronectin。而且, rLj-RGD3 通过人工的 matrigel 地下室膜阻止了 MCF-7/Adr 房间的侵略。在摘要, rLj-RGD3 可以在 multidrug 抵抗的乳癌治疗被用作潜在的药。 | Jihong Wang Rong Xiao Xin Liu Fenfang Wu Yue Pang Bo Feng Donghui Yang Qingwei Li | 2012 | Acta Biochimica et Biophysica Sinica2012，44，5： | 3 |
| 2 | Dynamic Response of Offshore Wind Turbine on 3×3 Barge Array Floating Platform under Extreme Sea Conditions显示文摘Offshore wind farm construction is nowadays state of the art in the wind power generation technology.However,deep water areas with huge amount of wind energy require innovative floating platforms to arrange and install wind turbines in order to harness wind energy and generate electricity.The conventional floating offshore wind turbine system is typically in the state of force imbalance due to the unique sway characteristics caused by the unfixed foundation and the high center of gravity of the platform.Therefore,a floating wind farm for 3×3 barge array platforms with shared mooring system is presented here to increase stability for floating platform.The NREL 5 MW wind turbine and ITI Energy barge reference model is taken as a basis for this work.Furthermore,the unsteady aerodynamic load solution model of the floating wind turbine is established considering the tip loss,hub loss and dynamic stall correction based on the blade element momentum(BEM)theory.The second development of AQWA is realized by FORTRAN programming language,and aerodynamic-hydrodynamic-Mooring coupled dynamics model is established to realize the algorithm solution of the model.Finally,the 6 degrees of freedom(DOF)dynamic response of single barge platform and barge array under extreme sea condition considering the coupling effect of wind and wave were observed and investigated in detail.The research results validate the feasibility of establishing barge array floating wind farm,and provide theoretical basis for further research on new floating wind farm. | LIU Qing-song MIAO Wei-pao YUE Min-nan LI Chun WANG Bo DING Qingwei | 2021 | China Ocean Engineering2021，35，2： | 2 |
| 3 | Lamprey serum can kill HeLa and NB4 tumor cells显示文摘 | Yinglun Han Yue Pang Tao Yu Rong Xiao Biyue Shi Peng Su Xin Liu Qingwei Li | 2014 | Acta Biochimica et Biophysica Sinica2014，46，7： | 1 |
| 4 | Unveiling the origin of anomalous low-frequency Raman mode in CVD-grown monolayer WS_(2)显示文摘Substrates provide the necessary support for scientific explorations of numerous promising features and exciting potential applications in two-dimensional (2D) transition metal dichalcogenides (TMDs). To utilize substrate engineering to alter the properties of 2D TMDs and avoid introducing unwanted adverse effects, various experimental techniques, such as high-frequency Raman spectroscopy, have been used to understand the interactions between 2D TMDs and substrates. However, sample-substrate interaction in 2D TMDs is not yet fully understood due to the lack of systematic studies by techniques that are sensitive to 2D TMD-substrate interaction. This work systematically investigates the interaction between tungsten disulfide (WS_(2)) monolayers and substrates by low-frequency Raman spectroscopy, which is very sensitive to WS_(2)-substrate interaction. Strong coupling with substrates is clearly revealed in chemical vapor deposition (CVD)-grown monolayer WS_(2) by its low-wavenumber interface mode. It is demonstrated that the enhanced sample-substrate interaction leads to tensile strain on monolayer WS_(2), which is induced during the cooling process of CVD growth and could be released for monolayer WS_(2) sample after transfer or fabricated by an annealing-free method such as mechanical exfoliation. These results not only suggest the effectiveness of low-frequency Raman spectroscopy for probing sample-substrate interactions in 2D TMDs, but also provide guidance for the design of high-performance devices with the desired sample-substrate coupling strength based on 2D TMDs. | Qian Xiang Xiaofei Yue Yanlong Wang Bin Du Jiajun Chen Shaoqian Zhang Gang Li Chunxiao Cong Ting Yu Qingwei Li Yuqi Jin | 2021 | Nano Research2021，14，11： | 1 |
| 5 | A novel protein tyrosine kinase Tec identified in lamprey, Lampetra japonica显示文摘蛋白质酷氨酸 kinase 侦探,一种非受体酷氨酸 kinase,首先被发现在 T 房间, B 房间,造血的房间,和肝房间被表示为细胞质的蛋白质。侦探被证明了是表明小径的 T 房间受体的一个批评调节的人。在现在的学习,侦探的一个相当或相同的事物在七鳃鳗被识别, Lampetra 装饰用的梨树。L 的全身的侦探 cDNA。装饰用的梨树(Lja 侦探) 包含 1923 bp 编码 641-amino 酸蛋白质的开的读框架。有典型脊椎动物侦探的 Lja 侦探的推出的氨基酸顺序的多排列证明它拥有侦探家庭蛋白质的所有保存领域,显示侦探的 ortholog 在现存无颚的脊椎动物存在。在种系发生的树上,那与 24 个相当或相同的事物被重建无颚并且 jawed 脊椎动物,从七鳃鳗和丑鱼的侦探作为单个 clade 被聚类。在外集团和 agnathan 侦探组之间的基因距离在外集团和 gnathostome 侦探组之间是比那靠近的,显示它的起源比任何 jawed 脊椎动物早远。在像淋巴细胞的房间和鳃的 Lja 侦探的 mRNA 层次被即时量的聚合酶链反应检测。结果证明它是显著地在有混合病原体的刺激下面的 upregulated。这结果被西方的污点分析进一步证实。所有这些结果显示 Lja 侦探在有免疫力的反应起一个重要作用。我们的数据将在无颚的脊椎动物为七鳃鳗侦探和它的免疫学的函数的进一步的学习提供引用。 | Ranran Li Peng Su Chang Liu Qiong Zhang Ting Zhu Yue Pang Xin Liu Qingwei Li | 2015 | Acta Biochimica et Biophysica Sinica2015，47，8： | 1 |
| 6 | HMGB1 is necessary for the development of lamprey显示文摘High mobility group box protein 1(HMGB1)with a highly conserved structure in chromatin is an abundant nonhistone protein,which is involved in nucleosome folding and assembly,chromatin formation,DNA binding,V(D)J recombination,DNA replication,gene transfer,cell differentiation,cell migration,neurotransmitters,cell death,immune response,and other important processes[1–4].So,it has important biological functions in animals.The function of hmgb1 has been extensively studied in jawed vertebrates but little in lampreys.Lampreys are members of the living agnathans,the most basal group of vertebrates.Lamprey is a considerable model organism in vertebrate evolution and developmental biology because of its special phylogenetic position and critical stage closely related to other animals.Many aspects of the hmgb1 in lampreys have not been revealed,such as evolution,distribution,and role.Therefore,in this paper,the evolution of hmgb1 was carried out,and the function of hmgb1 in lampreys was investigated by the morpholine antisense oligonucleotide(MO)technique.The data provide the foundation for the study of origins,evolution,and the function of hmgb1 in a jawless vertebrate.Linking evolution to function can shed more light on the role of the hmgb1 gene involved in development. | Jingjing Lu Jun Li Zihao Yan Xiaokun Wang Feng Lin Qinghua Ma Huaixiu Liu Haoran Guan Yueying Xun Xiaoying Kang Peijie Luan Xiao Feng Yue Pang Qingwei Li Yinglun Han | 2021 | Acta Biochimica et Biophysica Sinica2021，53，11： | 0 |
| 7 | Variable lymphocyte receptors play a key role in neutralization and opsonization in the lamprey显示文摘 | Hongyan Wen Yanjiao Dong Xiong Pan Zhao Tai Ying Zhou Qingwei Li Yue Pang | 2018 | Acta Biochimica et Biophysica Sinica2018，50，5： | 0 |
| 8 | Molecular cloning,expression pattern and phylogenetic analysis of Guanine nucleotide exchange factor Vav2 in lamprey,Lampetra japonica显示文摘The Guanine nucleotide exchange factor Vav2(Vav2) is a member of the Vav family that serves as an important regulators for the Rho family of Ras-related GTPases. In the current study, an ortholog(Lj-Vav2) of Vav2 was identified in the lamprey(Lampetra japonica). To elucidate the phylogenetic relationship of Vav2, the metazoan genome databases were analyzed to mine the ortholog of Vav. It was found that Vav2 genes were only existed in vertebrates and Lj-Vav2 was the original one found in agnathans. The evolutionary dynamics of conserved motifs of Vav2 were explored using combined amino acid sequence as markers, and it is revealed that the Calponin homology(CH) domain, Dbl-homologous(DH) domain, Pleckstrin homology(PH) domain, Cysteine-rich(C1)domains, Src homology 3(SH3) domains and Src homology 2(SH2) domain were conserved throughout the Vav2 gene family in vertebrates during gene evolution. Relative quantitative real-time PCR analysis showed that the LjVav2 was distributed in the heart, kidney, supraneural myeloid body, liver, gill and lymphocyte-like cells. The LjVav2 was found to be expressed in these tissues, and the level of which was upregulated in lymphocyte-like cells after the animal was stimulated with LPS. These results indicated that the Lj-Vav2 might be involved in the immune response of lymphocyte-like cells in lamprey. Meanwhile, our findings provided a foundation for further investigation of the function of Lj-Vav2 in the primary vertebrate. | HAN Yinglun LI Jun XU Lei MA Qinghua GOU Meng PANG Yue LIU Xin LI Qingwei | 2017 | Acta Oceanologica Sinica2017，36，12： | 0 |
| 9 | Immune-related gene expression in the early development of lamprey larva显示文摘 | Jun Li Yinglun Han Ting Zhu Yue Pang Qingwei Li | 2018 | Acta Biochimica et Biophysica Sinica2018，50，9： | 0 |
| 10 | Target-directed isolation and identification of a serum lectin from lamprey (Lampetra japonica) by chromatographys and MALDI-TOF/TOF显示文摘A 105-k Da polymer lectin was purified from lamprey(Lampetra japonica) serum by chromatography methods including cation ion-exchange chromatography with a SP-Sepharose TM XL column and size exclusion chromatography with a Superdex 200 column. The target fractions were collected according to the direction of hemagglutinating activity. The results revealed that the active fractions could adsorb on SP-Sepharose column and showed a 280 nm UV absorbance peak corresponding to molecular weights of 105 k Da in the following size exclusion chromatography. The target fractions with hemagglutinating activity were further checked by NativePAGE and SDS-PAGE. Two single bands at around 105 k Da and 35 k Da were displayed by two electrophoresis methods respectively, indicating that the protein exists as a trimer in solution. After Native-PAGE and SDS-PAGE,two bands were excised from the gels respectively and further identified by MALDI-TOF/TOF as serum lectin(gi:13094239). The lectin was able to agglutinate rabbit red blood cells(RRBCs) and sheep red blood cells(SRBCs) in vitro. The lectin isolated from lamprey serum in the current study might be helpful for deeply understanding the innate immune molecules dependent immune defence in jawless vertebrates which have been proved recently that they possess a lymphocyte-based system of anticipatory immunity with variable lymphocyte receptors as mediators. | HAN Yinglun GOU Meng SONG Xiaoping SONG Tao SHI Biyue PANG Yue LI Qingwei | 2018 | Acta Oceanologica Sinica2018，37，5： | 0 |
| 11 | Dynamic active sites on plasma engraved Ni hydroxide for enhanced electro-catalytic urea oxidation显示文摘The urea oxidization reaction(UOR)is an important anodic reaction in electro-catalytic energy conversion.However,the sluggish reaction kinetics and complex catalyst transformation in electrocatalysis require activity improvement and better mechanistic understanding of the state-of-the-art Ni(OH)_(2) catalyst.Herein,by utilizing low-temperature argon(Ar)plasma processing,tooth-wheel Ni(OH)_(2) nanosheets self-supported on Ni foam(Ni(OH)_(2)-Ar)are demonstrated to have improved UOR activity compared to conventional Ni(OH)_(2).The theoretical assessment confirms that the edge has a smaller cation vacancy formation energy than the basal plane,consequently explaining the structural formation.Operando and quasi-operando methods are employed to investigate the dynamic evolution of the Ni(OH)_(2) film in UOR.The crucial dehydrogenation products of Ni(OH)_(5)O^(-)intermediates are identified to be stable on the etched edge and explain the enhanced UOR in the low potential region.In addition,the dynamic active sites are monitored to elucidate the reaction mechanism in different potential ranges. | Dan Li Yuefeng Zhang Xiaomin Zhou Chao Huang Ying Wen Liangliang Liu Qingwei Li Yue Xu Yuzheng Wu Qingdong Ruan Yinghe Ma Fangyu Xiong Dezhi Xiao Pei Liu Guomin Wang Babak Mehrjou Bin Wang Hao Li Rongsheng Chen Hongwei Ni Zhiyuan Zeng Paul K.Chu | 2022 | Journal of Energy Chemistry2022，31，8： | 0 |
| 12 | A novel member of B-cell linker protein identified in lamprey, Lampetrajaponica显示文摘 | Yinglun Han | 2014 | Acta Biochimica et Biophysica Sinica2014，46，6： | 0 |
| 13 | Real-time and in-situ intracellular ATP assay with polyimidazolium brush-modified nanopipette显示文摘The level and dynamics of adenosine 5’-triphosphate(ATP) in single living cells are closely related to many kinds of important physiological and pathological processes. Herein, we report a nanosensor based on polyimidazolium brush(PimB)-modified nanopipette for intracellular ATP assay, which shows good selectivity, high spatiotemporal resolution and excellent stability.Both the specific supramolecular interaction between polyimidazolium and ATP, and the rationally tunable property of the ion transport at a confined nanopipette enable the selective assay of ATP even in complex intracellular environment. Moreover, the tiny tip(ca.300 nm) and the low polarized current(e.g., 1 nA) essentially make the as-prepared nanosensor safe to the living cells. The nanosensor is demonstrated to be useful for in-situ monitoring the basal ATP levels of chromaffin cells from wild-type mice and DJ-1 knockout mice, and for real-time monitoring the metabolism process of intracellular ATP. | Kailin Zhang Tianyi Xiong Fei Wu Qingwei Yue Wenliang Ji Ping Yu Lanqun Mao | 2020 | Science China Chemistry2020，63，7： | 0 |
| 14 | Automated Extraction for Water Bodies Using New Water Index from Landsat 8 OLI Images显示文摘The extraction of water bodies is essential for monitoring water resources,ecosystem services and the hydrological cycle,so analyzing water bodies from remote sensing images is necessary.The water index is designed to highlight water bodies in remote sensing images.We employ a new water index and digital image processing technology to extract water bodies automatically and accurately from Landsat 8 OLI images.Firstly,we preprocess Landsat 8 OLI images with radiometric calibration and atmospheric correction.Subsequently,we apply KT transformation,LBV transformation,AWEI nsh,and HIS transformation to the preprocessed image to calculate a new water index.Then,we perform linear feature enhancement and improve the local adaptive threshold segmentation method to extract small water bodies accurately.Meanwhile,we employ morphological enhancement and improve the local adaptive threshold segmentation method to extract large water bodies.Finally,we combine small and large water bodies to get complete water bodies.Compared with other traditional methods,our method has apparent advantages in water extraction,particularly in the extraction of small water bodies. | Pu YAN Yue FANG Jie CHEN Gang WANG Qingwei TANG | 2023 | Journal of Geodesy and Geoinformation Science2023，6，1： | 0 |
| 15 | Tongue-coating microbiome reflects cardiovascular health and determines outcome in blood pressure intervention显示文摘Tongue-coating microbiome,as a part of oral microbiome,is an important part of commensal microbes in human body.Despite technical difficulties in gaining functional insights using metagenomic approaches due to high levels of host DNA contaminations,a number of diseases have been found to be closely related to the alteration of oral microbial communities,including periodontal and caries diseases,cardiovascular disease,diabetes,rheumatoid arthritis. | Hairong Chen Yue Ma Min Li Qingwei Li Mengya Zhang Zixiong Wang Hongmei Liu Jun Wang Xiaolin Tong Yixin Zeng | 2023 | Journal of Genetics and Genomics2023，50，10： | 0 |
| 16 | 6-15 Plasma Discharge for In-situ Cleaning of SRF Cavities显示文摘Field emission occurred in superconducting radio-frequency(SRF)cavities is the major obstacle of the accelerators operating at high gradient,which partly caused by the inner surface contamination,such as the hydrocarbons and the absorbed residual gas.The plasma processing can be an effective method to solve the field emission issues.The properties of low temperature glow discharge,which was argon plasma with the chemically reactive oxygen,were investigated for the SRF cavities used for CADS project. | Wu Andong Yang Lei Li Yongming Yue Weiming Li Chunlong Xiong Pingran Guo Hao Song Yukun Chu Qingwei Jiang Tiancai Zhang Shengxue You Zhiming Zhang Shenghu He Yuan Zhao Hongwei | 2016 | IMP & HIRFL Annual Report2016，，1： | 0 |
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