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| 1 | Guiding T lymphopoiesis from pluripotent stem cells by defined transcription factors显示文摘Achievement of immunocompetent and therapeutic T lymphopoiesis from pluripotent stem cells(PSCs)is a central aim in T cell regenerative medicine.To date,preferentially reconstituting T lymphopoiesis in vivo from PSCs remains a practical challenge.Here we documented that synergistic and transient expression of Runx1 and Hoxa9 restricted in the time window of endothelial-to-hematopoietic transition and hematopoietic maturation stages in a PSC differentiation scheme(iR9-PSC)in vitro induced preferential generation of engraftable hematopoietic progenitors capable of homing to thymus and developing into mature T cells in primary and secondary immunodeficient recipients.Single-cell transcriptome and functional analyses illustrated the cellular trajectory of T lineage induction from PSCs,unveiling the T-lineage specification determined at as early as hemogenic endothelial cell stage and identifying the bona fide pre-thymic progenitors.The induced T cells distributed normally in central and peripheral lymphoid organs and exhibited abundant TCRαβrepertoire.The regenerative T lymphopoiesis restored immune surveillance in immunodeficient mice.Furthermore,gene-edited iR9-PSCs produced tumor-specific T cells in vivo that effectively eradicated tumor cells.This study provides insight into universal generation of functional and therapeutic T cells from the unlimited and editable PSC source. | Rongqun Guo Fangxiao Hu Qitong Weng Cui Lv Hongling Wu Lijuan Liu Zongcheng Li Yang Zeng Zhijie Bai Mengyun Zhang Yuting Liu Xiaofei Liu Chengxiang Xia Tongjie Wang Peiqing Zhou Kaitao Wang Yong Dong Yuxuan Luo Xiangzhong Zhang Yuxian Guan Yang Geng Juan Du Yangqiu Li Yu Lan Jiekai Chen Bing Liu Jinyong Wang | 2020 | Cell Research2020，30，1： | 7 |
| 2 | Magnetic beads-based multicolor colorimetric immunoassay for ultrasensitive detection of aflatoxin B_(1)显示文摘Aflatoxin B_(1)(AFB_(1))is one of the most toxic,mutagenic and carcinogenic mycotoxin,widely exists in contaminated food,grains and feedstuff products.In this study,a novel magnetic beads multicolor colorimetric immunoassay(MBMCIA)based on Au@Ag nanorods(Au@Ag NRs)is proposed to visual detect ultralow concentration of AFB_(1)with high-resolution by the naked-eye.To design the MBMCIA system,AFB_(1)-BSA conjugates were first coated on the surface of magnetic beads(MBs),then alkaline phosphatase(ALP)as a bridge between immunoassay a nd color reaction was used for catalytic hydrolysis of ascorbic acid-phosphate to generate reductive ascorbic acid.Finally,the yielded ascorbic acid could reduce silver ions to grow a silver coating on the surface of gold nanorods to generate Au@Ag NRs,which leads to the bule-shifted longitudinal absorption peak of Au NRs,accompanying with a series of perceptible color change.Under the optimal conditions,the proposed MBMCIA exhibited go od sensitivity and specificity for the detection of AFB_(1)with the detection limit as low as 5.7 pg/mL Meanwhile,the MBMCIA was also applied for the analysis of AFB_(1)in spiked wheat samples,the obtained recoveries range from 99.1%to 104.3%with relative standard deviation(RSD)less than 7.05%were acceptable.The proposed MBMCIA integrates separated,enriched,anti-interfe rence and signal read-out into one,which opens up a new avenue for an on-site visual food safety inspection or environmental monitoring. | Shan He Qitong Huang Ying Zhang Huifang Zhang Huifeng Xu Xun Li Xiaoming Ma | 2021 | Chinese Chemical Letters2021，32，4： | 3 |
| 3 | Investigation of kinetic and thermodynamic parameters of coal pyrolysis with model-free fitting methods显示文摘Kinetic analyses are important means for understanding the complicated coal pyrolysis process.This work conducted non-isothermal pyrolysis of four low-medium rank coals with thermogravimetric analyzer at different heating rates(5,10 and 20 K/min).Four model-free fitting approaches including KAS,OFW,Starink and Friedman methods were used to analyze pyrolysis kinetics of the coals.Thermodynamic parameters including enthalpy(ΔH)Gibbs free energy(ΔG)and entropy(ΔS)were calculated with the activation values(E)obtained with KAS method.For the kinetic analysis,the KAS,OFW and Starink methods give close E values over the whole conversion range,but the Friedman method shows more deviant results.The thermodynamic favorability of coal pyrolysis is discussed,bothΔH andΔG increase with conversion consistently with the variation of E,revealing that pyrolysis is endothermic and thermodynamic favorability is reduced with conversion rises.The minusΔS implies that coal structures evolve from disordered to organized ones as universally accepted.These results are expected to deepen our understanding and to optimize the conditions of coal pyrolysis. | Jingchong Yan Qitong Yang Li Zhang Zhiping Lei Zhanku Li Zhicai Wang Shibiao Ren Shigang Kang Hengfu Shui | 2020 | Carbon Resources Conversion2020，3，1： | 1 |
| 4 | Fabrication of extreme wettability surface for controllable droplet manipulation over a wide temperature range显示文摘Droplet controllable manipulation over a wide temperature range has promising applications in microelectronic heat dissipation, inkjet printing, and high temperature microfluidic system. However, the fabrication of a platform for controllable droplet manipulation using the methods commonly used in industry remains a tremendously challenge. The popular method of controlling droplets is highly dependent on external energy input and has relatively poor controllability in terms of droplet motion behaviors and manipulation environment, such as distance, velocity, direction and a wide temperature range. Here, we report a facile and industrially applicable method for preparing Al superhydrophobic (S-phobic) surfaces, which enables controlled droplet bouncing, evaporation, and transport over a wide temperature range. Systematic mechanistic studies are also investigated. Extreme wettability surfaces were prepared on Al substrate by a composite process of electrochemical mask etching and micro-milling. To investigate the evaporation process and thermal coupling characteristics, controlled evaporation and controlled bouncing of droplet in a wide temperature range were conducted. Based on the evaporation regulation and bouncing mechanism of droplets on an extreme wettability surface, by using Laplace pressure gradients and temperature gradients, we realized controlled transport of droplets with confluence, split-flow, and gravity-resistant transport over a wide temperature range, offering a potential platform for a series of applications, such as new drug candidates and water collection. | Chengsong Shu Qitong Su Minghao Li Zhenbin Wang Shaohui Yin Shuai Huang | 2022 | International Journal of Extreme Manufacturing2022，4，4： | 1 |
| 5 | Excitation and propagation of surface plasmon polaritons on a non-structured surface with a permittivity gradient显示文摘Accompanied by the rise of plasmonic materials beyond those based on noble metals and the development of advanced materials processing techniques,it is important to understand the plasmonic behavior of materials with large-scale inhomogeneity(such as gradient permittivity materials)because they cannot be modeled simply as scatterers.In this paper,we theoretically analyze the excitation and propagation of surface plasmon polaritons(SPPs)on a planar interface between a homogeneous dielectric and a material with a gradient of negative permittivity.We demonstrate the following:(i)free-space propagating waves and surface waves can be coupled by a gradient negative-permittivity material and(ii)the coupling can be enhanced if the material permittivity variation is suitably designed.This theory is then verified by numerical simulations.A direct application of this theory,‘rainbow trapping’,is also proposed,considering a realistic design based on doped indium antimonide.This theory may lead to various applications,such as ultracompact spectroscopy and dynamically controllable generation of SPPs. | Xi Wang Yang Deng Qitong Li Yijing Huang Zilun Gong Kyle B Tom Jie Yao | 2016 | Light(Science & Applications)2016，5，1： | 0 |
| 6 | Distinct enhancer-promoter modes determine Sox2 regulation in mouse pluripotent cells显示文摘Sox2 is a key transfer factor for maintaining pluripotency and self-renewal in embryonic stem cells(ESCs),1 though the mechanism of its transcriptional regulation in ESCs has not been fully addressed.Distal enhancer-promoter interactions are vital for Sox2 transcription activity in mammals.However,how these diverse interactions individually influence Sox2 gene regulation in mouse ESCs remains unclear.Previous studies found that three distal enhancers(termed E1,E2,and E3)interact with Sox2 promoter?(Fig.1A;Fig.S1A,and Table S1). | Lei Huang Xiusheng Zhu Qing Li Dashuai Kong Qitong Huang Jing Luo Siyuan Kong Yanling Peng Yubo Zhang | 2024 | Genes & Diseases2024，11，1： | 0 |
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