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| 1 | Abnormal expression of bHLH3 disrupts a flavonoid homeostasis network,causing differences in pigment composition among mulberry fruits显示文摘Mulberry fruits with high concentrations of anthocyanins are favored by consumers because of their good taste,bright color,and high nutritional value.However,neither the regulatory mechanism controlling flavonoid biosynthesis in mulberry nor the molecular basis of different mulberry fruit colors is fully understood.Here,we report that a flavonoid homeostasis network comprising activation and feedback regulation mechanisms determines mulberry fruit color.In vitro and in vivo assays showed that MYBA-bHLH3-TTG1 regulates the biosynthesis of anthocyanins,while TT2L1 and TT2L2 work with bHLH3 or GL3 and form a MYB-bHLH-WD40(MBW)complex with TTG1 to regulate proanthocyanidin(PA)synthesis.Functional and expression analyses showed that bHLH3 is a key regulator of the regulatory network controlling mulberry fruit coloration and that MYB4 is activated by MBW complexes and participates in negative feedback control of the regulatory network to balance the accumulation of anthocyanins and proanthocyanidins.Our research demonstrates that the interaction between bHLH3 and MYB4 in the homeostasis regulatory network ensures that the fruits accumulate desirable flavonoids and that this network is stable in pigmentrich mulberry fruits.However,the abnormal expression of bHLH3 disrupts the balance of the network and redirects flavonoid metabolic flux in pale-colored fruits,resulting in differences in the levels and proportions of anthocyanins,flavones,and flavonols among differently colored mulberry fruits(red,yellow,and white).The results of our study reveal the molecular basis of the diversity of mulberry fruit colors. | Han Li Zhen Yang Qiwei Zeng Shibo Wang Yiwei Luo Yan Huang Youchao Xin Ningjia He | 2020 | Horticulture Research2020，7，1： | 8 |
| 2 | Genome-wide identification and expression analyses of cytochrome P450 genes in mulberry(Morus notabilis)显示文摘Cytochrome P450 S play critical roles in the biosynthesis of physiologically important compounds in plants.These compounds often act as defense toxins to prevent herbivory.In the present study,a total of 174 P450 genes of mulberry(Morus notabilis C.K.Schn) were identified based on bioinformatics analyses.These mulberry P450 genes were divided into nine clans and 47 families and were found to be expressed in a tissue-preferential manner.These genes were compared to the P450 genes in Arabidopsis thaliana.Families CYP80,CYP92,CYP728,CYP733,CYP736,and CYP749 were found to exist in mulberry,and they may play important roles in the biosynthesis of mulberry secondary metabolites.Analyses of the functional and metabolic pathways of these genes indicated that mulberry P450 genes may participate in the metabolism of lipids,other secondary metabolites,xenobiotics,amino acids,cofactors,vitamins,terpenoids,and poiyketides.These results provide a foundation for understanding of the structures and biological functions of mulberry P450 genes. | Bi Ma Yiwei Luo Ling Jia Xiwu Qi Qiwei Zeng Zhonghuai Xiang Ningjia He | 2014 | Journal of Integrative Plant Biology2014，56，9： | 5 |
| 3 | Progress of Jinping Underground laboratory for Nuclear Astrophysics(JUNA)显示文摘Jinping Underground laboratory for Nuclear Astrophysics(JUNA) will take the advantage of the ultra-low background of CJPL lab and high current accelerator based on an ECR source and a highly sensitive detector to directly study for the first time a number of crucial reactions occurring at their relevant stellar energies during the evolution of hydrostatic stars. In its first phase, JUNA aims at the direct measurements of^(25)Mg(p,γ)^(26)Al,^(19)F(p,α)^(16)O,^(13)C(α,n)^(16)O and ^(12)C(α,γ)^(16)O reactions. The experimental setup,which includes an accelerator system with high stability and high intensity, a detector system, and a shielding material with low background, will be established during the above research. The current progress of JUNA will be given. | WeiPing Liu ZhiHong Li JiangJun He XiaoDong Tang Gang Lian Zhu An JianJun Chang Han Chen QingHao Chen XiongJun Chen ZhiJun Chen BaoQun Cui XianChao Du ChangBo Fu Lin Gan Bing Guo GuoZhu He Alexander Heger SuQing Hou HanXiong Huang Ning Huang BaoLu Jia LiYang Jiang Shigeru Kubono JianMin Li KuoAng Li Tao Li YunJu Li Maria Lugaro XiaoBing Luo HongYi Ma ShaoBo Ma DongMing Mei YongZhong Qian JiuChang Qin Jie Ren YangPing Shen Jun Su LiangTing Sun WanPeng Tan Isao Tanihata Shuo Wang Peng Wang YouBao Wang Qi Wu ShiWei Xu ShengQuan Yan LiTao Yang Yao Yang XiangQing Yu Qian Yue Sheng Zeng HuanYu Zhang Hui Zhang LiYong Zhang NingTao Zhang QiWei Zhang Tao Zhang XiaoPeng Zhang XueZhen Zhang ZiMing Zhang Wei Zhao Zuo Zhao Chao Zhou | 2016 | Science China(Physics,Mechanics & Astronomy)2016，59，4： | 3 |
| 4 | Identification and characterization of genes involved in the jasmonate biosynthetic and signaling pathways in mulberry(Morus notabilis)显示文摘Jasmonate(JA) is an important phytohormone regulating growth, development, and environmental response in plants, particularly defense response against herbivorous insects. Recently, completion of the draft genome of the mulberry(Morus notabilis) in conjunction with genome sequencing of silkworm(Bombyx mori) provides an opportunity to study this unique plant‐herbivore interaction. Here, we identified genes involved in JA biosynthetic and signaling pathways in the genome of mulberry for the first time, with the majority of samples showing a tissue‐biased expression pattern. The analysis of the representative genes 12‐oxophytodienoic acid reductase(OPRs) and jasmonate ZIM‐domain(JAZs) was performed and the results indicated that the mulberry genome contains a relatively small number of JA biosynthetic and signaling pathway genes. A gene encoding animportant repressor, MnNINJA, was identified as an alternative splicing variant lacking an ethylene‐responsive element binding factor‐associated amphiphilic repression motif. Having this fundamental information will facilitate future functional study of JA‐related genes pertaining to mulberry‐silkworm interactions. | Qing Wang Bi Ma Xiwu Qi Qing Guo Xuwei Wang Qiwei Zeng Ningjia He | 2014 | Journal of Integrative Plant Biology2014，56，7： | 3 |
| 5 | Construction and Characterization of a Novel Recombinant Attenuated and Replication-Deficient Candidate Human Adenovirus Type 3 Vaccine:'Adenovirus Vaccine Within an Adenovirus Vector'显示文摘Human adenoviruses(HAd Vs)are highly contagious and result in large number of acute respiratory disease(ARD)cases with severe morbidity and mortality.Human adenovirus type 3(HAd V-3)is the most common type that causes ARD outbreaks in Asia,Europe,and the Americas.However,there is currently no vaccine approved for its general use.The hexon protein contains the main neutralizing epitopes,provoking strong and lasting immunogenicity.In this study,a novel recombinant and attenuated adenovirus vaccine candidate against HAd V-3 was constructed based on a commercially-available replication-defective HAd V-5 gene therapy and vaccine vector.The entire HAd V-3 hexon gene was integrated into the E1 region of the vector by homologous recombination using a bacterial system.The resultant recombinants expressing the HAd V-3 hexon protein were rescued in AD293 cells,identified and characterized by RT-PCR,Western blots,indirect immunofluorescence,and electron microscopy.This potential vaccine candidate had a similar replicative efficacy as the wild-type HAd V-3 strain.However,and importantly,the vaccine strain had been rendered replication-defective and was incapable of replication in A549 cells after more than twentygeneration passages in AD293 cells.This represents a significant safety feature.The mice immunized both intranasally and intramuscularly by this vaccine candidate raised significant neutralizing antibodies against HAd V-3.Therefore,this recombinant,attenuated,and safe adenovirus vaccine is a promising HAd V-3 vaccine candidate.The strategy of using a clinically approved and replication-defective HAd V-5 vector provides a novel approach to develop universal adenovirus vaccine candidates against all the other types of adenoviruses causing ARDs and perhaps other adenovirus-associated diseases. | Yuqian Yan Shuping Jing Liqiang Feng Jing Zhang Zhiwei Zeng Min Li Shan Zhao Junxian Ou Wendong Lan Wenyi Guan Xiaowei Wu Jianguo Wu Donald Seto Qiwei Zhang | 2021 | Virologica Sinica2021，36，3： | 1 |
| 6 | Germ cell sex prior to meiosis in the rainbow trout显示文摘Germ cells make two major decisions when they move from an indeterminate state to their final stage of gamete production.One decision is sexual commitment for sperm or egg production,and the other is to maintain mitotic division or entry into meiosis.It is unclear whether the two decisions are made as a single event or separate events,because there has been no evidence for the presence of germ cell sex prior to meiosis.Here we report direct evidence in the fish rainbow trout that gonia have distinct sexuality.We show that dazl expression occurs in both male and female gonia but exhibits differential intracellular distribution.More strikingly,we show that boule is highly expressed in male gonia but absent in female gonia.Therefore,mitotic gonia possess sex,sperm/egg decision and mitosis/meiosis decision are two independent events,and sperm/egg decision precedes mitosis/meiosis decision in rainbow trout,making this organism a unique vertebrate model for mechanistic understanding of germ cell sex differentiation and relationship between the two decisions. | Mingyou Li Qian Shen Foong Mei Wong Hongyan Xu Ni Hong Lingbing Zeng Lin Liu Qiwei Wei Yunhan Hong | 2011 | Protein & Cell2011，2，1： | 1 |
| 7 | Two homologous INDOLE-3-ACETAIVHDE (IAM) HYDROLASE genes are required for the auxin effects of IAM in Arabidopsis显示文摘Indole-3-acetamide(IAM)is the first confirmed auxin biosynthetic intermediate in some plant pathogenic bacteria.Exogenously applied IAM or production of IAM by overexpressing the bacterial iaaM gene in Arabidopsis causes auxin overproduction phenotypes.However,it is still inconclusive whether plants use IAM as a key precursor for auxin biosynthesis.Herein,we reported the isolation IAM HYDROLASE 1(IAMH1)gene in Arabidopsis from a forward genetic screen for IAM-insensitive mutants that display normal auxin sensitivities.IAMH1 has a close homolog named IAMH2 that is located right next to IAMH1on chromosomeⅣin Arabidopsis.We generated iamh1 iamh2 double mutants using our CRISPR/Cas9gene editing technology.We showed that disruption of the IAMH genes rendered Arabidopsis plants resistant to IAM treatments and also suppressed the iaaM overexpression phenotypes,suggesting that IAMH1 and IAMH2 are the main enzymes responsible for converting IAM into indole-3-acetic acid(IAA)in Arabidopsis.The iamh double mutants did not display obvious developmental defects,indicating that IAM does not play a major role in auxin biosynthesis under normal growth conditions.Our findings provide a solid foundation for clarifying the roles of IAM in auxin biosynthesis and plant development. | Yangbin Gao Xinhua Dai Yuki Aoi Yumiko Takebayashi Liping Yang Xiaorui Guo Qiwei Zeng Hanchuanzhi Yu Hiroyuki Kasahara Yunde Zhao | 2020 | Journal of Genetics and Genomics2020，47，3： | 0 |
| 8 | Platelet factor 4 induces bone loss by inhibiting the integrinα5-FAK-ERK pathway显示文摘Background:The effect of platelet factor 4(PF4)on bone marrow mesenchymal stem cells(BMMSCs)and osteoporosis is poorly understood.Therefore,this study aimed to evaluate the effects of PF4-triggered bone destruction in mice and determine the underlying mechanism.Methods:First,in vitro cell proliferation and cell cycle of BMMSCs were assessed using a CCK8 assay and flow cytometry,respectively.Osteogenic differentiation was confirmed using staining and quantification of alkaline phosphatase and Alizarin Red S.Next,an osteoporotic mouse model was established by performing bilateral ovariectomy(OVX).Furthermore,the PF4 concentrations were obtained using enzymelinked immunosorbent assay.The bone microarchitecture of the femur was evaluated using microCT and histological analyses.Finally,the key regulators of osteogenesis and pathways were investigated using quantitative real-time polymerase chain reaction and Western blotting.Results:Human PF4 widely and moderately decreased the cell proliferation and osteogenic differentiation ability of BMMSCs.Furthermore,the levels of PF4 in the serum and bone marrow were generally increased,whereas bone microarchitecture deteriorated due to OVX.Moreover,in vivo mouse PF4 supplementation triggered bone deterioration of the femur.In addition,several key regulators of osteogenesis were downregulated,and the integrinα5-focal adhesion kinase-extracellular signalregulated kinase(ITGA5-FAK-ERK)pathway was inhibited due to PF4 supplementation.Conclusions:PF4 may be attributed to OVX-i nduced bone loss triggered by the suppression of bone formation in vivo and alleviate BMMSC osteogenic differentiation by inhibiting the ITGA5-FAK-ERK pathway. | Wei Li Qiwei Zhang Ranli Gu Lijun Zeng Hao Liu | 2023 | Animal Models and Experimental Medicine2023，6，6： | 0 |
| 9 | Dynamic changes in transposable element and gene methylation in mulberry (Morus notabilis) in response to Botrytis cinerea显示文摘DNA methylation has been proposed to regulate plant stress resistance.However,the dynamic changes in DNA methylation in woody plants and their correlations with pathogenic responses are not fully understood.Here,we present single-base maps of the DNA methylomes of mulberry(Morus notabilis)leaves that were subjected to a mock treatment or inoculation with Botrytis cinerea.Compared with the former,the latter showed decreased mCG and mCHG levels and increased mCHH levels.DNA methylation inhibitors reduced resistance gene methylation levels and enhanced mulberry resistance,suggesting that the hypomethylation of resistance genes affects mulberry resistance to B.cinerea.Virus-induced gene silencing of MnMET1 enhanced the expression of mulberry-resistance genes,thereby increasing the plant’s resistance to B.cinerea.We also found that MITEs play a dominant role in controlling DNA methylation levels.MITEs appear to be the main sources of 24-nt siRNAs that regulate gene expression through the RNA-directed DNA methylation pathway. | Youchao Xin Bi Ma Qiwei Zeng Wenmin He Meiling Qin Ningjia He | 2021 | Horticulture Research2021，8，1： | 0 |
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