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| 1 | C/EBPα regulates SIRT1 expression during adipogenesis显示文摘SIRT1 在 adipogenesis 起一个重要作用,但是 SIRT1 怎么在 adipogenesis 被调整,大部分是未知的。在这研究,我们证明 SIRT1 蛋白质和 mRNA 层次与 CCAAT/enhancer-binding 蛋白质伪一起被增加(C/EBP 伪) 在 adipocyte 区别期间。C/EBP 伪,然而并非 C/EBP 伪 p 30,在 HeLa 房间和 3T3-L1 的激活的 SIRT1 倡导者 preadipocytes。而且, C/EBP 伪 upregulated SIRT1 mRNA 和蛋白质在 Soas2 房间以一种 p53 独立的方式在 HeLa 铺平房间和增加的 SIRT1 表示。在 preadipocytes, C/EBP 伪 upregulated SIRT1 蛋白质水平的宫外的表示并且导致的 C/EBP 伪击倒 SIRT1 的减少蛋白质水平。而且由倡导者删除分析,胶化移动试金和染色质 immunoprecipitation,我们发现了那 C/EBP,伪在一个一致 C/EBP 伪绑定地点跳了到 SIRT1 倡导者。这些数据证明那 C/EBP 伪在 adipogenesis 期间调整 SIRT1 表示由对 SIRT1 倡导者直接有约束力。 | Qihuang Jin Fang Zhang Tingting Yan Zhen Liu Chunxi Wang Xinjian Ge Qiwei Zhai | 2010 | Cell Research2010，20，4： | 8 |
| 2 | SIRT1 Improves Insulin Sensitivity under Insulin-Resistant Conditions by Repressing PTP1B显示文摘 | Cheng Sun Fang Zhang Xinjian Ge Tingting Yan Xingmiao Chen Xianglin Shi Qiwei Zhai | 2007 | Cell Metabolism2007，，4： | 3 |
| 3 | Wld^(S),Nmnats and axon degeneration-progress in the past two decades显示文摘A chimeric protein called Wallerian degeneration slow(Wld^(S))was first discovered in a spontaneous mutant strain of mice that exhibited delayed Wallerian degeneration.This provides a useful tool in elucidating the mechanisms of axon degeneration.Over-expression of WldS attenuates the axon degeneration that is associated with several neurodegenerative disease models,suggesting a new logic for developing a potential protective strategy.At molecular level,although Wld^(S)is a fusion protein,the nicotinamide mononucleotide adenylyl transferase 1(Nmnat1)is required and sufficient for the protective effects of Wld^(S),indicating a critical role of NAD biosynthesis and perhaps energy metabolism in axon degeneration.These findings challenge the proposed model in which axon degeneration is operated by an active programmed process and thus may have important implication in understanding the mechanisms of neurodegeneration.In this review,we will summarize these recent findings and discuss their relevance to the mechanisms of axon degeneration. | Yan Feng Tingting Yan Zhigang He Qiwei Zhai | 2010 | Protein & Cell2010，1，3： | 2 |
| 4 | Expression of phenylalanine ammonia lyase as an intracellularly free and extracellularly cell surface-immobilized enzyme on a gut microbe as a live biotherapeutic for phenylketonuria显示文摘Phenylketonuria(PKU),a disease resulting in the disability to degrade phenylalanine(Phe)is an inborn error with a 1 in 10,000 morbidity rate on average around the world which leads to neurotoxicity.As an potential alternative to a protein-restricted diet,oral intake of engineered probiotics degrading Phe inside the body is a promising treatment,currently at clinical stage II(Isabella,et al.,2018).However,limited transmembrane transport of Phe is a bottleneck to further improvement of the probiotic’s activity.Here,we achieved simultaneous degradation of Phe both intracellularly and extracellularly by expressing genes encoding the Phe-metabolizing enzyme phenylalanine ammonia lyase(PAL)as an intracellularly free and a cell surface-immobilized enzyme in Escherichia coli Nissle 1917(EcN)which overcomes the transportation problem.The metabolic engineering strategy was also combined with strengthening of Phe transportation,transportation of PAL-catalyzed trans-cinnamic acid and fixation of released ammonia.Administration of our final synthetic strain TYS8500 with PAL both displayed on the cell surface and expressed inside the cell to the Pah^(F263S)PKU mouse model reduced blood Phe concentration by 44.4%compared to the control Ec N,independent of dietary protein intake.TYS8500 shows great potential in future applications for PKU therapy. | Yu Jiang Bingbing Sun Fenghui Qian Feng Dong Chongmao Xu Wuling Zhong Rui Huang Qiwei Zhai Yu Jiang Sheng Yang | 2023 | Science China(Life Sciences)2023，66，1： | 1 |
| 5 | Involvement of the Ubiquitin-Proteasome System in the Early Stages of Wallerian Degeneration显示文摘 | Qiwei Zhai Jing Wang Anna Kim Qing Liu Ryan Watts Eric Hoopfer Timothy Mitchison Liqun Luo Zhigang He | 2003 | Neuron2003，，2： | 1 |
| 6 | Investigating the function of Akt by tet-off inducible expression system显示文摘A tet-off inducible cell line named BBT derived from BA/F3β cell line was constructed and the effect of this inducible expression system was significant when detected by tet-off responded luciferase reporter gene assay. Then tet-off responded Akt expression plasmid was transfected into BBT cells, and the stable cell lines were screened. The result of Northern blot showed that the expression of akt was significantly inducible. The clone with the best inducible effect was selected and named BBA for investigating the function of Akt. We found that Akt could significantly inhibit zinc-induced decrease of cell viability when assayed by MTT method. And the flow cytometric analysis showed that Akt could markedly repress zinc-induced apoptosis. | Qiwei Zhai Hongbin Ji Zhongcheng Zheng Lanying Sun Xinyuan Liu | 2001 | Chinese Science Bulletin2001，46，3： | 1 |
| 7 | SIRT1 Improves Insulin Sensitivity under Insulin-Resistant Conditions by Repressing PTP1B显示文摘 | Cheng Sun Fang Zhang Xinjian Ge Tingting Yan Xingmiao Chen Xianglin Shi Qiwei Zhai | 2007 | Cell Metabolism2007，，4： | 1 |
| 8 | Nmnat2 delays axon degeneration in superior cervical ganglia dependent on its NAD synthesis activity显示文摘 | Tingting Yan Yan Feng Jin Zheng Xinjian Ge Yi Zhang Dongmei Wu Jian Zhao Qiwei Zhai | 2009 | Neurochemistry International2009，，1： | 1 |
| 9 | Involvement of the Ubiquitin-Proteasome System in the Early Stages of Wallerian Degeneration显示文摘 | Qiwei Zhai Jing Wang Anna Kim Qing Liu Ryan Watts Eric Hoopfer Timothy Mitchison Liqun Luo Zhigang He | 2003 | Neuron2003，，2： | 1 |
| 10 | DC field effect on dielectric property of Ba(Zr x Ti 1? x )O 3 ceramics显示文摘 | Bo Shen Qiwei Zhang Jiwei Zhai Zhengkui Xu | 2012 | Ceramics International2012，，： | 1 |
| 11 | RNA的poly(A)化修饰为真核细胞提供能量代谢稳态调控的便捷通道显示文摘文章简介本研究首次论证了poly(A)化可作为一个标签,实现真核细胞蛋白质合成的整体把控,而且首次把RNA转运引入到能量代谢稳态调控领域,为能量代谢紊乱相关疾病防治及药物研发提供了新研究视野。 | Peipei Shan Guangjian Fan Lianhui Sun Jinqin Liu Weifang Wang Chen Hu Xiaohong Zhang Qiwei Zhai Xiaoyu Song Liu Cao Yongping Cui 张胜萍 王传贵 | 2018 | 科学新闻2018，0，4： | 0 |
| 12 | Interleukin-2 mediates the peripheral analgesia of interferon alpha显示文摘Interferon-α (IFN-α) and interleukin-2 (IL-2) are crucial cytokines in immune system. They also play an important role in nerve system. It has been reported that IL-2 can induce the central analgesia. It is demonstrated that IFN-α also can induce the peripheral analgesia, which can be blocked by naloxone as IL-2. Furthermore, the analgesic effect of IFN-α is reversible by monoclonal anti-IL-2 antibody. In vitro experiments show that IFN-α significantly increases the production of IL-2 in a dose dependent manner. These data suggest that IL-2 mediates the peripheral analgesia of IFN-α. | Li Li Qiwei Zhai Wei Tang Tong Wu Zhongcheng Zheng Xinyuan Liu | 1999 | Chinese Science Bulletin1999，44，3： | 0 |
| 13 | RELAXOR FERROELECTRIC MATERIALS FOR MICROWAVE TUNABLE APPLICATIONS显示文摘With strong dependences of dielectric constant on external applied electric fields,relaxor barium zirconium titanate(BaZr_(x)Ti_(1-x)O_(3)or BZT)and barium stannate titanate(BaS_(n)xTi_(1-x)O_(3)or BTS),in both bulk ceramic and thinfilm forms,are increasingly being recognized as potential candidates of microwave tunable materials for device applications.This paper is aimed to review the recent progress in understanding the dielectric properties(such as tunability,dielectric loss and dielectric constant)of these relaxor materials.However,due to their relatively high dielectric constant and loss tangent,pure Ba(Zr,Ti)O_(3) and Ba(Sn,Ti)O_(3) do not fully satisfy the requirements of practical device applications.Therefore,various strategies have been developed to improve the dielectric properties of these two groups of relaxor materials.In this paper,wefirst discussed the dielectric tunability characteristics of pure Ba(Zr,Ti)O_(3) and Ba(Sn,Ti)O_(3) and then summarized the strategies that have been used,including(i)small amount acceptor or donor doping(such as rare-earth ions and transition metal ions)and(ii)forming composites with low loss and low dielectric constant microwave dielectric materials(such as MgO,MgTiO_(3) and so on).At the same time,the relationship between relaxor behavior and dielectric tunability was also discussed. | QIWEI ZHANG JIWEI ZHAI LING BING KONG | 2012 | Journal of Advanced Dielectrics2012，2，1： | 0 |
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