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| 1 | Production of bioactive ginsenoside compound K in metabolically engineered yeast显示文摘 | Xing Yan Yun Fan Wei Wei Pingping Wang Qunfang Liu Yongjun Wei Lei Zhang Guoping Zhao Jianmin Yue Zhihua Zhou | 2014 | Cell Research2014，24，6： | 81 |
| 2 | Characterization of Panax ginseng UDP- Glycosyltransferases Catalyzing Protopanaxatriol and Biosyntheses of Bioactive Ginsenosides F1 and Rhl in Metabolically Engineered Yeasts显示文摘人参皂甙,在人参(人参属人参) 的主要 pharmacologically 活跃的自然混合物,主要是 protopanaxadiol (PPD ) 和 protopanaxatriol (PPT ) 的 glycosylated 产品。uridine diphosphate glycosyltransferase (UGT ) 催化 PPT 生产 PPT 类型人参皂甙,还都没被报导。这里,我们显示出那 UGTPg1,它 regio 明确地被表明了到 glycosylate C20 -- PPD 的 OH,另外明确地 glycosylates C20 -- PPT 到的 OH 生产 bioactive 人参皂甙 F1。我们报导四新奇 UGT 基因从 P.ginseng 孤立的 characterizationof,分享高推出的氨基酸身份(>84%) 与 UGTPg1。我们明确地表明那 UGTPg100 glycosylates C6 -- PPT 的 OH 催化 PPT 生产 bioactive 人参皂甙 Rh1,和 UGTPg101 生产 F1,从 F1 由人参皂甙 Rg1 的产生列在后面。然而, UGTPg102 和 UGTPg103 被发现不在 PPT 上举办可检测的活动。通过结构的建模和指导地点的 mutagenesis,我们识别了可以在决定他们的活动和底层 regio 特性起重要作用的这些 UGT 的几关键氨基酸。而且,我们由介绍遗传上设计的生产 PPT 小径和 UGTPg1 或 UGTPg100 构造了酵母 recombinants 到 biosynthesize F1 和 Rh1。我们的学习经由合成生物学策略在酵母为 bioactive PPT 类型人参皂甙在人参属工厂,和 providesa 声音生产途径揭示 PPT 类型人参皂甙的可能的 biosynthetic 小径。 | Wei Wei Pingping Wang Yongjun Wei Qunfang Liu Chengshuai Yang Guoping Zhao Jianmin Yue Xing Yan Zhihua Zhou | 2015 | Molecular Plant2015，8，9： | 37 |
| 3 | Toxicity of the brominated flame retardant tris-(2,3-dibromopropyl) isocyanurate in zebrafish (Danio rerio)显示文摘Tris-(2,3-dibromopropyl) isocyanurate (TBC) is a heterocyclic brominated flame retardant that was recently detected in the environment in China.TBC is semi-volatile and can accumulate in the lipid of some species,but little is known about its effect on aquatic organisms.We exposed adult zebrafish to 0,0.25,1 and 4mg/L TBC for 28 d and measured the effect on survival,growth,histopathology,hormone levels,enzyme activity,and gene expression.TBC exposure had no effect on survival or growth.We observed significant damage to the liver and gill,including hepatocellular swelling and fatty degeneration in the liver as well as proliferation and edema of epithelial cells in the gills.In addition,exposure to 4mg/L TBC induced proliferation of goblet cells in the intestine of both sexes,acellular areas in the testis,and thinly scattered vitellogenic granules in vitellogenic oocytes.TBC exposure had no effect on the levels of thyroid hormones,testosterone,estradiol,liver superoxide dismutase activity,malondialdehyde content,and brain cholinesterase activity.By contrast,hepatic vitellogenin and cytochrome P4501A gene expression was significantly down-regulated in both male and female zebrafish in response to TBC exposure.Our results suggest that exposure to TBC causes a variety of potential reproductive and endocrine toxic effects. | ZHANG Xu LI Juan CHEN MinJie WU Le ZHANG Chi ZHANG Jie ZHOU QunFang LIANG Yong | 2011 | Chinese Science Bulletin2011，56，15： | 13 |
| 4 | Intranasal administration of tetrabromobisphenol A bis(2-hydroxyethyl ether) induces neurobehavioral changes in neonatal Sprague Dawley rats显示文摘Tetrabromobisphenol A(TBBPA) and its derivatives are now being highly concerned due to their emerging environmental occurrence and deleterious effects on non-target organisms.Considering the potential neurotoxicity of TBBPA derivatives which has been demonstrated in vitro, what could happen in vivo is worthy of being studied. Tetrabromobisphenol A bis(2-hydroxyethyl ether)(TBBPA-BHEE), a representative TBBPA derivative, was selected for a21-day exposure experiment on neonatal Sprague Dawley(SD) rats through intranasal administration. The neurobehavioral, histopathological changes, and differentially expressed genes based on RNA microarray were investigated to evaluate the neurological effects of this chemical. The results indicated that TBBPA-BHEE exposure significantly compromised the motor co-ordination performance and the locomotor activities(p < 0.05). The neurobehavioral phenotype could be attributed to the obvious histopathological changes in both cerebrum and cerebellum, such as neural cell swelling, microglial activation and proliferation. A total of 911 genes were up-regulated, whereas 433 genes were down-regulated. Gene set enrichment analysis showed multiple signaling pathways, including ubiquitin-mediated proteolysis and wingless-int(Wnt) signaling pathway etc. were involved due to TBBPA-BHEE exposure. The gene ontology enrichment analysis showed the basic cellular function and the neurological processes like synaptic transmission were influenced. The toxicological effects of TBBPA-BHEE observed in this study suggested the potential neuronal threaten from unintended exposure,which would be of great value in the biosafety evaluation of TBBPA derivatives. | Qian S.Liu Na Liu Zhendong Sun Qunfang Zhou Guibin Jiang | 2018 | Journal of Environmental Sciences2018，30，1： | 7 |
| 5 | Rapid decolorization of water soluble azo-dyes by nanosized zero-valent iron immobilized on the exchange resin显示文摘Nanosized zero-valent iron (NZVI) supported on the cation exchange resin was synthesized and applied to decompose some water soluble azo dyes. The decomposition efficiency for azo dyes was evaluated by using the aqueous suspensions and parked column of this material. Batch experiments indicated that this novel material exhibited excellent degradation ability for 0.05 g·L1 of Acid Orange 7, Acid Orange 8, Acid Orange 10, Sunset Yellow, and Methyl Orange, with decolorization ratio up to 95% in 4 min; pH value was the key factor for degradation and H+ was one of the reactants; adsorption of azo dyes onto the material existed at the beginning but reduced gradually until disappearing completely. For the packed column system, 58%~90% of azo dyes were decomposed in the 1st circle of solution passing through the column, and the adsorption onto the materials could accelerate the degradation azo dyes with the increasing reaction time. During the degradation process, Fe2+, the product of NZVI, was exchanged to the resin again and could be reduced to Fe0 by KBH4 for reusing. The 10th refreshed NZVI possessed reductive activity up to 90% of the newly systhesized NZVI. Decomposing pollutants in the aqueous solution with columns packed with NZVI immobilized on the cation exchange resin is a promising technology that can solve the reclaiming and refreshing problem of NZVI. | ZHAO ZongShan LIU JingFu TAI Chao ZHOU QunFang HU JingTian JIANG GuiBin | 2008 | Science China Chemistry2008，51，2： | 6 |
| 6 | Effects of cadmium,17 β-estradiol and their interaction in the male Chinese loach (Misgurnus anguillicaudatus)显示文摘To assess the interaction effect between cadmium (Cd) and 17-estradiol (E2),male Chinese loaches (Misgurnus anguillicaudatus) were exposed to E2 (1 g/L) and Cd (50 or 500 g/L) alone and in combination using a semi-static waterborne exposure system.The effects of E2 on the accumulation and distribution of Cd,as well as the effects of Cd on vitellogenin (Vtg) synthesis induced by E2,were investigated.Cd mainly accumulated in the kidneys,liver,intestines,and gills,with little amounts in muscles,bones,and gonads.Co-exposure with E2 did not change the main targets for Cd.E2 could induce Vtg synthesis in male Chinese loaches,and co-exposure with 50 or 500 g/L Cd could inhibit the Vtg induced by 1 g/L E2.Compared with the results reported in the literature,it can be concluded that factors such as fish species,Cd dosage,and manner of exposure might make contributions to the interaction between Cd and E2.Our results also suggested that male Chinese loaches are susceptible to Cd,and can be recommended as a potential sentinel species to study the ecotoxicology of heavy metals. | LÜ XueFei LIU FeiYuan ZHOU XiaoPing ZHOU QunFang DENG YuLin | 2012 | Chinese Science Bulletin2012，57，8： | 3 |
| 7 | Amphiphilic drug-drug conjugate for cancer therapy with combination of chemotherapeutic and antiangiogenesis drugs显示文摘The progression and metastasis of solid tumors strongly rely on the process of forming nascent blood vessels.However,using angiogenesis inhibitors alone does not meet the cancer treatment needs.Herein,we used the amphiphilic drug-drug conjugate(ADDC)strategy to fabricate a new drug conjugate with the combination of chemotherapeutic drug and antiangiogenesis drug together.With one-step esterification of hydrophilic floxuridine(FUDR)and hydrophobic pseudolaric acid B(PAB),the conjugate was synthesized.The amphiphilic property of FUDR-PAB conjugate induced the self-assembly to form nanoparticles in water.From further in vitro and in vivo experiments,this FUDR-PAB conjugate does not only have a high antitumor effect,but also shows efficient antianiogenesis property.These results offer a promising ADDC strategy for designing drugs with combination of chemotherapeutic drug and antiangiogenesis drug together. | Mo Sun Qiuhui Qian Leilei Shi Li Xu Qunfang Liu Linzhu Zhou Xinyuan Zhu Jian-Min Yue Deyue Yan | 2020 | Science China Chemistry2020，63，1： | 3 |
| 8 | Alterations of endogenous metabolites in urine of rats exposed to decabromodiphenyl ether using metabonomic approaches显示文摘There is large usage of polybrominated diphenyl ethers(PBDEs) especially for decabromodiphenyl ether(BDE-209, Deca-BDE) in controlling the risks of fire. The toxicological effects of PBDEs are worth being concerned about. Female SD rats were daily gavaged with BDE-209 ether at the dose of 100 mg/kg for 20 days. Histological observation was performed for the screening of the target organs for BDE-209 exposure. The distribution and metabolism of PBDEs in the exposed main organs were evidenced by HRGC-HRMS. Alterations of the endogenous metabolite concentrations in urine were investigated using metabonomic approaches based on1H NMR spectrum. Histopathological changes including serious edema in kidney, hepatocellular spotty necrosis and perivasculitis in liver indicated that BDE-209 caused potential influences on endogenous metabolism in the exposed liver and the kidney. BDE-209 was found to be highly accumulated in lipid, ovary, kidney and liver after 20 days' exposure. Occurrence of other lower brominated PBDEs in the rats demonstrated that reductive debromination process happened in vivo. Hydroxylated and methoxylated–BDEs, as metabolism products, were also detected in the rat tissues. A total of 12 different endogenous metabolites showed obvious alterations in urine from the exposed rats, indicating the disturbance of the corresponding internal biochemical processes induced by BDE-209 exposure. These findings in vivo suggested the potential health risk might be of concern due to the toxicological effects of BDE-209 as a ubiquitous compound in the environment. | Weijin Yang Jianjie Fu Thanh Wang Hanxia Liu Yawei Wang Qunfang Zhou Guibin Jiang | 2014 | Journal of Environmental Sciences2014，26，4： | 3 |
| 9 | Development of retinol-binding protein 4 immunocolloidal gold fast test strip using high-sensitivity monoclonal antibodies generated by DNA immunization显示文摘DNA 免疫是为高亲密关系的 monoclonal 抗体产生的一个有效方法。这里,我们对松香油绑定蛋白质 4 描述几高质量的 monoclonal 抗体(mAbs ) 的产生(RBP4 ) ,为肾反常和机能障碍的一个重要标记,与 DNA 的一个联合方法, priming 和蛋白质增加。产生的 mAbs 能与高敏感并且用这些 mAbs 绑在 RBP4,一块 immunocolloidal 金牌快测试长带被构造。长带能交上结果 < 5 min 并且与大约 1 ng/ml 的察觉限制是很敏感的。小规模的临床的测试表明这脱衣的结果根据在市场上当前可得到的一个标记酶的 immunosorbent 试金工具包的好。因而,它能为在诊所的更方便、更快的 RBP4 决心是有用的。 | Chao Bian Fang Zhang Feng Wang Zhiyang Ling Min Luo Hongqiang Wu Yizhuo Sun Junhui Li B ingnan Li Jingyan Zhu Linna Tang Yanyan Zhou Qunfang Shi Yongyong Ji Lin Tian Guomei Lin Yu Fan Niansong Wang Bing Sun | 2010 | Acta Biochimica et Biophysica Sinica2010，42，12： | 2 |
| 10 | Effects of nanoscale quantum dots in male Chinese loaches (Misgurnus anguillicaudatus):Estrogenic interference action,toxicokinetics and oxidative stress显示文摘Quantum dots (QDs) have received more and more attention as a novel example of nanocrystals due to their unique fluorescent characteristics. Recently, the toxicity and the potential environmental effects of QDs have become a research hotspot. In this work, in vivo endocrine disrupting effect, toxicokinetics and oxidative stress of QDs were characterized following the intraperitoneal dosing in Chinese loaches. Vitellogenin (Vtg) levels induced by E2 decreased significantly when administrated with the mixture of QDs and E2, which was consistent with the observations of histopathology in testes. The release of free Cd2+ from QDs and the non-specific adsorption of E2 by QDs might be the joint factors contributing to the inhibition of Vtg expression induced by E2 in the male Chinese loaches. In the muscle, bone, intestines, blood and testis, CdSe QDs reached the maximal concentration (Cmax) in approximately 1-h postinjection and subsequently presented downtrend with the prolonged time. Whereas, there were even increasing tendencies of CdSe QDs' concentrations in the liver and kidney. It is educible that CdSe QDs can be persistent at least for 7 days, indicating the overall half-life of CdSe QDs in the fish body is very long. The measurement of hepatic superoxide dismutase (SOD) activity and reduced glutathione (GSH) content indicate that QDs have smaller effects on the antioxidative system of the organisms compared with free Cd2+ due to the effective prevention of the release of Cd by PEG coating of QDs. The comprehensive evaluation of QDs' toxicity in the present study provides an essential and general framework towards more focused research on the elucidation of the biological effects of QDs in vivo. | HongCheng Li WenRu Luo Yong Tao Yuan Wu XueFei Lv QunFang Zhou GuiBin Jiang | 2009 | Science China Chemistry2009，52，10： | 2 |
| 11 | Analysis of human urine metabolites using SPE and NMR spectroscopy显示文摘Nuclear magnetic resonance (NMR) spectroscopic analysis of metabonome/metabolome has widespread applications in biomedical science researches. However, most of NMR resonances for urinary metabolites remain to be fully assigned. In the present study, human urine samples from two healthy volunteers were pre-treated with C18 solid-phase extraction and the resultant 5 sub-fractions were subjected to one- and two-dimensional NMR studies, including 1H J-Resolved, 1H-1H COSY, 1H-1H TOCSY, 1H-13C HSQC, and HMBC 2D NMR. More than 70 low molecular weight metabolites were identified, and complete assignments of 1H and 13C resonances including many complex coupled spin systems were obtained. | YANG WeiJin WANG YaWei ZHOU QunFang TANG HuiRu | 2008 | Science China Chemistry2008，51，3： | 2 |
| 12 | Progress in the toxicological researches for quantum dots显示文摘Quantum dots (QDs) have received more and more attention as a novel example of nanomaterials. Due to their unique fluorescent characteristics,quantum dots have been successfully applied in biotech-nology and medicine applications. Recently,the toxicity and the potential environmental effects of QDs have become a research hotspot. In this paper,toxicological effects of QDs are reviewed,and the prospects and research directions are given based on the analysis of this research field. | LI HongCheng ZHOU QunFang LIU Wei YAN Bing ZHAO Yibing JIANG GuiBin | 2008 | Science China Chemistry2008，51，5： | 2 |
| 13 | Vitellogenic responses of male Chinese loach (Misgurnus anguillicaudatus) exposed to the individual or binary mixtures of 17β-estradiol and nonylphenol显示文摘Male Chinese loaches were exposed to 17β-estradiol (E2) and nonylphenol (NP) both singly and in combination for 42 days using semi-static waterborne exposure system. Plasma vitellogenin (Vtg) was chosen as determining endpoint. The results demonstrated that 0.5 μg/L E2 could induce the enhancement of Vtg contents in male Chinese loaches after exposure for 21 days, which showed a time-related increasing manner; NP was also estrogenic to male Chinese loach, and the vitellogenic responses showed in a time-and dose-related manner, which was less potent than that of E2. The bi- nary mixtures of E2 and NP can significantly elicit the production of Vtg in male Chinese loaches, which was more potent than that of individual compounds, and Vtg induced in the binary mixture groups was higher than the summation of Vtg induced in the corresponding single-compound groups at the same concentration. | LU XueFei ZHOU QunFang SONG MaoYong JIANG GuiBin SHAO Jing | 2007 | Chinese Science Bulletin2007，52，24： | 2 |
| 14 | Mechanism of gold nanoparticle induced simultaneously increased PCR efficiency and specificity显示文摘Gold nanoparticles(AuNPs)have been reported to induce faster heat transfer of polymerase chain reaction(PCR)solution to enhance PCR efficiency.AuNPs can also increase the specificity of PCR by functioning analogously to single-stranded DNA binding protein(SSB).However,the simple structure of AuNPs makes it difficult to determine how AuNPs affect PCR efficiency and specificity.This study aimed to elucidate the effect of AuNPs on PCR efficiency by altering the denaturation times and annealing temperatures.In addition,comparative study of the effect of AuNPs on PCR inhibition caused by two competitive primers allowed investigation of the mechanisms of AuNP-enhanced PCR accuracy.Finally,heat-treated salmon sperm DNA was used to evaluate whether AuNPs could eliminate the inhibitory PCR effect caused by DNA impurities.This study demonstrated that enhanced thermal conductivity by AuNPs was the main mechanism for increased PCR efficiency and specificity.AuNPs promoted efficient double-stranded DNA template unwinding and dissociation between mismatched primers,DNA fragments and template,and enhanced PCR efficiency and specificity simultaneously.Thus,this significant finding suggests the future use of AuNP-assisted PCR in different fields,especially in rapid clinical diagnosis and screening by PCR. | LIN Yan LI Jia YAO Jing LIANG Yong ZHANG Jie ZHOU QunFang JIANG GuiBin | 2013 | Chinese Science Bulletin2013，58，36： | 2 |
| 15 | Preparation of polyaniline coating on a stainless-steel wire using electroplating and its application to the determination of six aromatic amines using headspace solid-phase microextraction 显示文摘 | Huang Minjia Tai Chao Zhou Qunfang Jiang Guibin | 2004 | Journal of Chromatography A2004，，1048： | 1 |
| 16 | Environmental pollution,a hidden culprit for health issues显示文摘The environmental and health impacts from the massive discharge of chemicals and subsequent pollution have been gaining increasing public concern.The unintended exposure to different pollutants,such as heavy metals,air pollutants and organic chemicals,may cause diverse deleterious effects on human bodies,resulting in the incidence and progression of different diseases.The article reviewed the outbreak of environmental pollution-related public health emergencies,the epidemiological evidence on certain pollution-correlated health effects,and the pathological studies on specific pollutant exposure.By recalling the notable historical life-threatening disasters incurred by local chemical pollution,the damning evidence was presented to criminate certain pollutants as the main culprit for the given health issues.The epidemiological data on the prevalence of some common diseases revealed a variety of environmental pollutants to blame,such as endocrine-disrupting chemicals(EDCs),fine particulate matters(PMs)and heavy metals.The retrospection of toxicological studies provided illustrative clues for evaluating ambient pollutant-induced health risks.Overall,environmental pollution,as the hidden culprit,should answer for the increasing public health burden,and more efforts are highly encouraged to strive to explore the cause-and-effect relationships through extensive epidemiological and pathological studies. | Hanqing Xu Yang Jia Zhendong Sun Jiahui Su Qian S.Liu Qunfang Zhou Guibin Jiang | 2022 | Eco-Environment & Health2022，1，1： | 1 |
| 17 | Bacterial cellulose/gelatin composites:In site preparation and glutalaldehyde treatment显示文摘 | Chen Yuanxi Zhou Xiaodong Lin Qunfang | 2014 | Cellulose2014，21，14： | 1 |
| 18 | Excretion of PFOA and PFOS in male rats during a subchronic exposure 显示文摘 | CUI Lin LIAO Chunyang ZHOU Qunfang | 2010 | Archives of Environmental Contamination and Toxicology2010，58，1： | 1 |
| 19 | Organotin pollution in China: an overview of the current state and potential health risk 显示文摘 | CAO Dandan JIANG Guibin ZHOU Qunfang | 2009 | Journal of Environmental Management2009，90，1： | 1 |
| 20 | Effects of silver nanoparticles on the development and histopathology biomarkers of Japanese medaka ( Oryzias latipes ) using the partial-life test显示文摘 | Yuan Wu Qunfang Zhou Hongcheng Li Wei Liu Thanh Wang Guibin Jiang | 2009 | Aquatic Toxicology2009，，2： | 1 |
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