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| 1 | 华北地区不同规模畜禽养殖场粪便中抗生素抗性基因污染特征显示文摘为调查华北地区畜禽养殖环境抗生素抗性基因(ARGs)污染特征及影响因素,采集河北省和天津市不同规模的养猪场和养鸡场新鲜粪便样品,分析粪便中ARGs污染水平及有机质与ARGs含量的相关性。实时荧光定量PCR结果显示,不同种类畜禽养殖场粪便ARGs的相对丰度有显著区别,鸡粪中sul和erm基因的相对丰度高于猪粪,而编码核糖体保护蛋白的tet基因(4.24×10^-3~5.85×10^-1)在猪粪和鸡粪中的相对丰度无明显差异,均显著高于sul(1.07×10^-4~2.26×10^-1)、erm(6.36×10^-4~2.62×10^-1)以及编码外派泵蛋白和酶抑制剂的tet基因(1.24×10^-4~5.41×10^-2)。不同规模的养猪场粪便ARGs污染水平趋势为:中型>大型>小型,而不同规模养鸡场粪便中ARGs相对丰度无显著性差异(P=0.551);此外,正交偏最小二乘判别(OPLS-DA)与典型相关分析(CCA)结果显示,畜禽粪便中编码核糖体保护蛋白的tet基因(tetM、tetO和tetW)相对丰度与有机碳(OC)和有机氮(ON)含量高度相关(VIP>1),sul基因则与OC/ON明显相关。综上,粪便中有机质和生物可利用碳氮比是影响畜禽养殖业ARGs污染水平的重要因素。 | 邹威 金彩霞 魏闪 Ramasamy Rajesh Kumar 周启星 | 2020 | 农业环境科学学报2020，39，11： | 17 |
| 2 | 间充质的干细胞的隔离和描述源于人的胎盘组织显示文摘 AIM: To explore the feasibility of placenta tissue as a reliable and efficient source for generating mesenchymal stem cells (MSC). METHODS: MSC were generated from human placenta tissue by enzymatic digestion and mechanical dissociation. The placenta MSC (PLC-MSC) were characterized for expression of cell surface markers, embryonic stem cell (ECS) gene expression and their differentiation ability into adipocytes and osteocytes. The immunosuppressive properties of PLC-MSC on resting and phytohemagglutinin (PHA) stimulated allogenic T cells were assessed by means of cell proliferation via incorporation of tritium thymidine (3H-TdR). RESULTS: The generated PLC-MSC appeared as spindle-shaped cells, expressed common MSC surface markers and ESC transcriptional factors. They also differen-tiated into adipogenic and osteogenic lineages when induced. However, continuous cultivation up to passage 15 caused changes in morphological appearance and cellular senescence, although the stem cell nature of their protein expression was unchanged. In terms of their immunosuppressive properties, PLC-MSC were unable to stimulate resting T cell proliferation; they inhibited the PHA stimulated T cells in a dose dependent manner through cell to cell contact. In our study, MSC generated from human placenta exhibited similar mesenchymal cell surface markers; MSC-like gene expression pattern and MSC-like differentiation potential were comparable to other sources of MSC. CONCLUSION: We suggest that placenta tissues can serve as an alternative source of MSC for future experimental and clinical studies. | Shalini Vellasamy Pratheep Sandrasaigaran Sharmili Vidyadaran Elizabeth George Rajesh Ramasamy | 2012 | World Journal of Stem Cells2012，4，6： | 5 |
| 3 | 骨头的 Immunophenotype 和区别能力从 CBA/Ca, ICR 和 Balb/c 的导出髓的间充质的干细胞老鼠显示文摘 AIM:To assess the capacity to isolate and expand mesenchymal stem cells(MSC)from bone marrow of CBA/Ca,ICR and Balb/c mice. METHODS:Bone marrow of tibia and femur were flushed,cultured and maintained in supplemented Dulbecco’s modified Eagle’s medium.MSC immunophenotype of cultures were tracked along increasing passages for positivity to CD106,Sca-1 and CD44 and negativity to CD45,CD11b and MHC classⅡ.Differentiation capacity of MSC towards osteogenic and adipo-genic lineages were also assessed. RESULTS:MSC were successfully cultured from bone marrow of all 3 strains,albeit differences in the temporal expression of certain surface antigens.Their differentiation into osteocytes and adipocytes were also observed. MSC from all 3 mouse strains demonstrated a shift from a haematopoietic phenotype(CD106-CD45+CD11b+Sca-1low)to typical MSC phenotype(CD106+CD45-CD11b-Sca-1high)with increasing passages. CONCLUSION:Information garnered assists us in the decision of selecting a mouse strain to generate MSC from for downstream experimentation. | Yin Yin Ooi Zul'atfi Rahmat Shinsmon Jose Rajesh Ramasamy Sharmili Vidyadaran | 2013 | World Journal of Stem Cells2013，5，1： | 3 |
| 4 | Mesenchymal Stem Cells Inhibit Dendritic Cell Differentiation and Function by Preventing Entry Into the Cell Cycle显示文摘 | Rajesh Ramasamy Henrietta Fazekasova Eric W.-F. Lam Inês Soeiro Giovanna Lombardi Francesco Dazzi | 2007 | Transplantation2007，，1： | 2 |
| 5 | Electromagnetic field exposure as a plausible approach to enhance the proliferation and differentiation of mesenchymal stem cells in clinically relevant scenarios显示文摘Mesenchymal stem/stromal cell(MSC)-based therapy has been regarded as one of the most revolutionary breakthroughs in the history of modern medicine owing to its myriad of immunoregulatory and regenerative properties.With the rapid progress in the fields of osteo-and musculoskeletal therapies,the demand for MSC-based treatment modalities is becoming increasingly prominent.In this endeavor,researchers around the world have devised new and innovative techniques to support the proliferation of MSCs while minimizing the loss of hallmark features of stem cells.One such example is electromagnetic field(EMF)exposure,which is an alternative approach with promising potential.In this review,we present a critical discourse on the efficiency,practicability,and limitations of some of the relevant methods,with insurmountable evidence backing the implementation of EMF as a feasible strategy for the clinically relevant expansion of MSCs. | Haslinda Abdul HAMID Vahid Hosseinpour SARMADI Vivek PRASAD Rajesh RAMASAMY Azizi MISKON | 2022 | Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)2022，23，1： | 1 |
| 6 | The role of mesenchymal stem cells in haemopoiesis显示文摘 | Francesco Dazzi Rajesh Ramasamy Sarah Glennie Simon P. Jones Irene Roberts | 2005 | Blood Reviews2005，，3： | 1 |
| 7 | Bone marrow-derived mesenchymal stem cells modulate BV2 microglia responses to lipopolysaccharide显示文摘 | Yin Yin Ooi Rajesh Ramasamy Zul’atfi Rahmat Hemavathy Subramaiam Shi Wei Tan Maha Abdullah Daud Ahmad Israf Sharmili Vidyadaran | 2010 | International Immunopharmacology2010，，12： | 1 |
| 8 | Mesenchymal Stem Cells Inhibit Dendritic Cell Differentiation and Function by Preventing Entry Into the Cell Cycle显示文摘 | Rajesh Ramasamy Henrietta Fazekasova Eric W.-F. Lam Inês Soeiro Giovanna Lombardi Francesco Dazzi | 2007 | Transplantation2007，，1： | 1 |
| 9 | 显示文摘 | Rajesh N P Kannan V Ashok M Sivaji K Santhana R P Ramasamy P | 2004 | J Crystal Growth2004，262，14： | 1 |
| 10 | Preliminary study on overproduction of reactive oxygen species by neutrophils in diabetes mellitus显示文摘AIM:To assess the amount and pattern of reactive oxygen species(ROS)production in diabetic patientderived neutrophils.METHODS:Blood samples from type 2 diabetes mellitus(DM)patients and volunteers(controls)were subjected to neutrophil isolation and the assessment of neutrophil oxidative burst using chemiluminescence assay.Neutrophils were activated by using phorbol myristate acetate(PMA)and neutrophils without activation were kept as a negative control.The chemilu-minescence readings were obtained by transferring cell suspension into a 1.5 m L Eppendorf tube,with PMA and luminol.Reaction mixtures were gently vortexed and placed inside luminometer for a duration of 5 min.RESULTS:Our results showed that in the resting condition,the secretion of ROS in normal non-diabetic individuals was relatively low compared to diabetic patients.However,the time scale observation revealed that the secreted ROS declined accordingly with time in non-diabetic individuals,yet such a reduction was not detected in diabetic patients where at all the time points,the secretion of ROS was maintained at similar magnitudes.This preliminary study demonstrated that ROS production was significantly higher in patients with DM compared to non-diabetic subjects in both resting and activated conditions.CONCLUSION:The respiratory burst activity of neutrophils could be affected by DM and the elevation of ROS production might be an aggravating factor in diabetic-related complications. | Noridzzaida Ridzuan Cini Mathew John Pratheep Sandrasaigaran Maryam Maqbool Lee Chuen Liew Jonathan Lim Rajesh Ramasamy | 2016 | World Journal of Diabetes2016，7，13： | 0 |
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