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| 1 | Hepatogenic differentiation of human mesenchymal stem cells from adipose tissue in comparison with bone marrow mesenchymal stem cells显示文摘AIM: To investigate and compare the hepatogenic transdifferentiation of adipose tissue-derived stem cells (ADSC) and bone marrow-derived mesenchymal stem cells (BMSC) in vitro. Transdifferentiation of BMSC into hepatic cells in vivo has been described. Adipose tissue represents an accessible source of ADSC, with similar characteristics to BMSC.METHODS: BMSCs were obtained from patients under-going total hip arthroplasty and ADSC from human adi-pose tissue obtained from lipectomy. Cells were grown in medium containing 15% human serum. Cultures were serum deprived for 2 d before cultivating under similar pro-hepatogenic conditions to those of liver develop-ment using a 2-step protocol with sequential addition of growth factors, cytokines and hormones. Hepatic differ-entiation was RT-PCR-assessed and liver-marker genes were immunohistochemically analysed. RESULTS: BMSC and ADSC exhibited a fibroblastic mor-phology that changed to a polygonal shape when cells differentiated. Expression of stem cell marker Thy1 de-creased in differentiated ADSC and BMSC. However, the expression of the hepatic markers, albumin and CYPs increased to a similar extent in differentiated BMSC and ADSC. Hepatic gene activation could be attributed to in-creased liver-enriched transcription factors (C/EBPβ and HNF4α), as demonstrated by adenoviral expression vec-tors.CONCLUSION: Mesenchymal stem cells can be induced to hepatogenic transdifferentiation in vitro. ADSCs have a similar hepatogenic differentiation potential to BMSC, but a longer culture period and higher proliferation ca-pacity. Therefore, adipose tissue may be an ideal source of large amounts of autologous stem cells, and may become an alternative for hepatocyte regeneration, liver cell transplantation or preclinical drug testing. | Raquel Taléns-Visconti Ana Bonora Ramiro Jover Vicente Mirabet Francisco Carbonell José Vicente Castell María José Gómez-Lechón | 2006 | World Journal of Gastroenterology2006，12，36： | 35 |
| 2 | Development of a Rapid Risk and Impact Assessment Tool to Enhance Response to Environmental Emergencies in the Early Stages of a Disaster: A Tool Developed by the European Multiple Environmental Threats Emergency NETwork (EMETNET) Project显示文摘Every year,numerous environmental disasters and emergencies occur across the globe with far-reaching impacts on human health and the environment.The ability to rapidly assess an environmental emergency to mitigate potential risks and impacts is paramount.However,collating the necessary evidence in the early stages of an emergency to conduct a robust risk assessment is a major challenge.This article presents a methodology developed to help assess the risks and impacts during the early stages of such incidents,primarily to support the European Union Civil Protection Mechanism but also the wider global community in the response to environmental emergencies.An online rapid risk and impact assessment tool has also been developed to promote enhanced collaboration between experts who are working remotely,considering the impact of a disaster on the environment and public health in the short,medium,and long terms.The methodology developed can support the appropriate selection of experts and assets to be deployed to affected regions to ensure that potential public health and environmental risks and impacts are mitigated whenever possible.This methodology will aid defensible decision making,communication,planning,and risk management,and presents a harmonized understanding of the associated impacts of an environmental emergency. | Emma-Jane Goode Eirian Thomas Owen Landeg Raquel Duarte-Davidson Lisbeth Hall Jolanda Roelofs Sjors Schulpen Arnout De Bruin Elisabeth Wigenstam Birgitta Liljedahl Annica Waleij Louise Simonsson Ann Göransson Nyberg | 2021 | International Journal of Disaster Risk Science2021，12，4： | 2 |
| 3 | Successful living donor intestinal transplantation in crossmatch positive recipients: Initial experience显示文摘Sensitized patients tend to have longer waiting times on the deceased donor list and are at increased risk of graft loss from acute or chronic rejection compared to non-sensitized candidates. Desensitization protocols are utilized to decrease the levels of alloantibodies and to convert an initial positive cross-match to prospective donors into a negative crossmatch. These procedures are mostly available in the setting of living donation. Due to the elective nature of the procedure, desensitization protocols can be extended until the desire result is obtained prior to transplantation. We present two cases of successful desensitization protocol applied to living donor intestinal transplant candidates that converted to negative cross-match to their donors. We present two cases of intestinal transplant candidates with a potential living donor to whom they are sensitized. Both cases underwent successful transplantation after desensitization protocol. No evidence of humoral rejection has occurred in either recipient. Living donor intestinal transplantation in sensitized recipients against the prospective donors provides the ability to implement a desensitization protocol to convert to negative crossmatch. | Raquel Garcia-Roca Ivo G Tzvetanov Hoonbae Jeon Elisabeth Hetterman Jose Oberholzer Enrico Benedetti | 2016 | World Journal of Gastrointestinal Surgery2016，8，1： | 2 |
| 4 | A method for the determination of volatile N-nitrosamines in food by HS-SPME-GC-TEA 显示文摘 | Raquel A Felix G R Susanne R | 2005 | Food Chemistry2005，91，1： | 1 |
| 5 | Higher rates of autism and attention deficit/hyperactivity disorder in American children:Are food quality issues impacting epigenetic inheritance?显示文摘In the United States,schools offer special education services to children who are diagnosed with a learning or neurodevelopmental disorder and have difficulty meeting their learning goals.Pediatricians may play a key role in helping children access special education services.The number of children ages 6-21 in the United States receiving special education services increased 10.4%from 2006 to 2021.Children receiving special education services under the autism category increased 242%during the same period.The demand for special education services for children under the developmental delay and other health impaired categories increased by 184%and 83%respectively.Although student enrollment in American schools has remained stable since 2006,the percentage distribution of children receiving special education services nearly tripled for the autism category and quadrupled for the developmental delay category by 2021.Allowable heavy metal residues remain persistent in the American food supply due to food ingredient manufacturing processes.Numerous clinical trial data indicate heavy metal exposures and poor diet are the primary epigenetic factors responsible for the autism and attention deficit hyperactivity disorder epidemics.Dietary heavy metal exposures,especially inorganic mercury and lead may impact gene behavior across generations.In 2021,the United States Congress found heavy metal residues problematic in the American food supply but took no legislative action.Mandatory health warning labels on select foods may be the only way to reduce dietary heavy metal exposures and improve child learning across generations. | Renee J Dufault Raquel A Crider Richard C Deth Roseanne Schnoll Steven G Gilbert Walter J Lukiw Amanda L Hitt | 2023 | World Journal of Clinical Pediatrics2023，12，2： | 1 |
| 6 | Importance of patientspecific intraoperative guides in complex maxillofacial reconstruction 显示文摘 | DENNIS R RAQUEL G M PETER B | 2013 | J Cranio Maxill Surg2013，41，5： | 1 |
| 7 | Effect of seed size and testa colour on saponin content of Spanish lentil seed显示文摘 | RAQUEL G R KEITH R P MALCOLM E R | 1997 | Food Chem1997，58，3： | 1 |
| 8 | Partitioning of ethoxylated alkylphenol surfactants in microemulsion-oil-water Systems显示文摘 | Nelson M Raquel A Alain G | 1995 | Colloids and Surfaces A: Physicochemical and Engineering Aspects1995，100，25： | 1 |
| 9 | Cervix assessment for the management of labor induction: Reliability of cervi- cal length and Bishop score determined by residents 显示文摘 | Raquel G S Daniel O Daniel G C | 2015 | Journal of Obstetrics & Gynaecology Research2015，41，3： | 1 |
| 10 | New genus-specific primers for the PCR identification of members of the genera Pseudonocardia and Saccharopolyspora显示文摘 | RAQUEL M LGNACIO G OLGA G | 1999 | International Journal of Systematic Bacteriology1999，49，： | 1 |
| 11 | New genus-specific primers for the PCR identification of novel isolates of the genera Nocardiopsis and Saccharothrix显示文摘 | RAQUEL M LGNACIO G OLGA G | 2002 | International Journal of Systematic and Evolutionary Microbiology2002，52，： | 1 |
| 12 | Extraordinaryoptical reflection from sub-wavelength cylinder ar-rays 显示文摘 | Raquel G M Marine L Juan J S | 2006 | Opt Express2006，14，9： | 1 |
| 13 | Fusion of multi- spectral and panchromatic images using improved HIS and PCA mergers based on wavelet decomposition 显示文摘 | MARIA G A JOSE L S RAQUEL G | 2004 | IEEE Trans Geoseienee and Remote Sensing2004，42，6： | 1 |
| 14 | A pilot study of β-interferon for treatment of patients with chronic hepatitis B who failed to respond to α-interferon显示文摘 | Raquel M Gregorio G Inmaculada F | 2002 | J Hepatology2002，37，： | 1 |
| 15 | A method for the determination of volatile N-nitrosamines in food by HS-SPME-GC-TEA显示文摘 | Felix G R Susanne R | 2005 | Food Chemistry2005，91，1： | 1 |
| 16 | Feelings of well being in elderly people : relationship to physical activity and physical function 显示文摘 | Nuria G Olga M Raquel M | 2009 | Arch Gerontol Gefiatr2009，48，3： | 1 |
| 17 | Solid liq- uid equilibrium of substrates and products of the enzymatic synthesis of ampicillin显示文摘 | Marcelo P A R Geisa A L Raquel L C G | 2010 | AIChE Journal2010，56，6： | 1 |
| 18 | Determination of total selenium and selenium distribution in the milk phases in commercial cow's milk by HG-AAS显示文摘 | Cocar M N Raquel D G Adela B B Jos6 A C Jos6 M F Pilar B B | 2005 | Analytical and Bioanalytical Chemistry2005，381，6： | 1 |
| 19 | Sepicanin A-A new geranyl flavanone from Artocarpus sepicanus with activity against methicillin-resistant Staphylococcus aureus ( MRSA ) 显示文摘 | Radwan M M Raquel R G Manly S P | 2009 | Phytochemistry Lett2009，2，4： | 1 |
| 20 | Respirometric assays at fixed and process temperatures to monitor composting process 显示文摘 | Raquel B G Felicitas V L Bolasell F G | 2005 | Bioresour Technol2005，96，10： | 1 |
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