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| 1 | Multiplexed Biosensing Diagnostic Platforms Detecting Autoantibodies to Tumor-Associated Antigens from Exosomes Released by CRC Cells and Tissue Samples Showed High Diagnostic Ability for Colorectal Cancer显示文摘Colorectal cancer(CRC)is the second leading cause of cancer-related death worldwide.The five-year survival rate of CRC patients depends on the stage at diagnosis,being higher than 80%when CRC is diagnosed in the early stages but lower than 10%when CRC is diagnosed in advanced stages.Autoantibodies against specific CRC autoantigens(tumor-associated antigens(TAAs))in the sera of patients have been widely demonstrated to aid in early diagnosis.Thus,we herein aim to identify autoantigens target of autoantibodies specific to CRC that possess a significant ability to discriminate between CRC patients and healthy individuals by means of liquid biopsy.To that end,we examined the protein content of the exosomes released by five CRC cell lines and tissue samples from CRC patients by means of immunoprecipitation coupled with mass spectrometry analysis.A total of 103 proteins were identified as potential autoantigens specific to CRC.After bioinformatics and meta-analysis,we selected 15 proteins that are more likely to be actual CRC autoantigens in order to evaluate their role in CRC prognosis by Western blot(WB)and immunohistochemistry(IHC).We found dysregulation at the protein level for 11 of these proteins in both tissue and plasma exosome samples from patients,along with an association of nine of these proteins with CRC prognosis.After validation,all but one showed a statistically significant high diagnostic ability to distinguish CRC patients and individuals with premalignant lesions from healthy individuals,either by luminescence Halotag-based beads,or by a multiplexed biosensing platform involving the use of magnetic microcarriers as solid support modified with covalently immobilized Halotag fusion proteins constructed for CRC detection.Taken together,our results highlight the usefulness of the approach defined here to identify the TAAs specific to chronic diseases;they also demonstrate that the measurement of autoantibody levels in plasma against the TAAs identified here could be integrated into a point-of-care(POC)device for CRC detection with high diagnostic ability. | Ana Montero-Calle Itziar Aranguren-Abeigon María Garranzo-Asensio Carmen Poves María Jesús Fernández-Aceñero Javier Martínez-Useros Rodrigo Sanz Jana Dziaková Javier Rodriguez-Cobos Guillermo Solís-Fernández Eloy Povedano Maria Gamella Rebeca Magnolia Torrente-Rodríguez Miren Alonso-Navarro Vivian de los Ríos J.Ignacio Casal Gemma Domínguez Ana Guzman-Aranguez Alberto Peláez-García JoséManuel Pingarrón Susana Campuzano Rodrigo Barderas | 2021 | Engineering2021，7，10： | 1 |
| 2 | Development of a fast instrumental method for the analysis of pharmaceuticals in environmental and wastewaters based on ultra high performance liquid chromatography (UHPLC)- tandem mass spectrometry ( MS/MS ) 显示文摘 | REBECA L S MIRA P DAMIA B | 2011 | Chemosphere2011，85，8： | 1 |
| 3 | Development of a murine model of airway infammation and remodeling in experimental asthma显示文摘 | REBECA F I LAURA N N NADIA S B | 2009 | Arch Bronconeumol2009，45，9： | 1 |
| 4 | Severe acute kidney injury associated with non-steroidal anti-inflammatory drugs in cirrhosis: A case-control study显示文摘 | Chiara Elia Isabel Graupera Rogelio Barreto Elsa Solà Rebeca Moreira Patricia Huelin Xavier Ariza Cristina Solé Elisa Pose Anna Baiges Núria Fabrellas Esteban Poch Javier Fernández Vicente Arroyo Pere Ginès | 2015 | Journal of Hepatology2015，，3： | 1 |
| 5 | Bangladeshi backyard poultry raisers' perceptions and practices related to zoonotic transmission of avian influenza显示文摘 | Rebeca S NA Rimi S Azad | 2012 | Journal of Infection in Developing Countreies2012，6，2： | 1 |
| 6 | Tailor-made polymer electrolytes based upon ionic liquids and their application in all-plastic electrochromie devices显示文摘 | Rebeca M Francisco A Haritz S | 2006 | Electrochemistry Communications2006，8，3： | 1 |
| 7 | Biosynthesis and degradation of H2O2 by vaginal lactobacilli显示文摘 | REBECA M JUAN E S | 2010 | Appl Environ Microbiol2010，76，2： | 1 |
| 8 | Occurrence and distribution of pharmaceuticals in surface water,suspended solids and sediments of the Ebro River Basin,Spain显示文摘 | Silva B F D Jelic A Rebeca L S Mozeto A A Petrovic M Barceló D | | 0，，8： | 1 |
| 9 | Occurrence anddistribution of pharmaceuticals in surface water,suspended solidsand sediments of the Ebro river basin,Spain显示文摘 | Bianca F S Aleksandra J Rebeca L S | 2011 | Chemosphere2011，85，8： | 1 |
| 10 | Effect of cooking on olive oil quality attributes显示文摘 | Carla S P Santos Rebeca Cruz Sara C Cunha | 2013 | Food Research International2013，，54： | 1 |
| 11 | PORPHOBILINOGEN DEAMINASE deficiency alters vegetative and reproductive development and causes lesions in arabidopsis显示文摘 | Victor Q Raquel S M Rebeca G B Andrea H Maria R P Jose L M | 2013 | PLoS ONE2013，8，53： | 1 |
| 12 | Miroena-the levnorgestrl intrauterine system (20μg/day)显示文摘 | Rebeca S | 2003 | Journal of Drug Evaluation2003，1，： | 1 |
| 13 | Abbreviated method for urea kinetic modeling in continuous ambulatory peritoneal dialysis patients显示文摘 | Francis D Rebeca S Cosme C | 1993 | Peritoneal Dialysis International1993，13，12： | 1 |
| 14 | Impact of mass screening for gluten-sensitive enteropathy in working population显示文摘AIM:To assess:(1)frequency and clinical relevanceof gluten sensitive enteropathy(GSE)detected by serology in a mass screening program;(2)sensitivity of antitransglutaminase(tTGA)and antiendomysium antibodies(EmA);and(3)adherence to gluten-free diet(GFD)and follow-up. METHODS:One thousand,eight hundred and sixtyeight subjects recruited from an occupational health department underwent analysis for tTGA and EmA and, if positive,duodenal biopsy,DQ2/DQ8 genotyping, clinical feature recording,blood tests,and densitometry were performed.Since>98%of individuals had tTGA <2 U/mL,this value was established as the cut-off limit of normality and was considered positive when confirmed twice in the same sample.Adherence to a GFD and follow up were registered. RESULTS:Twenty-six(1.39%)subjects had positive tTGA and/or EmA,and 21 underwent biopsy:six Marsh Ⅲ(oneⅢa,fourⅢb,oneⅢc),nine MarshⅠand six Marsh 0(frequency of GSE 1:125).The sensitivity of EmA for GSE was 46.6%(11.1%for MarshⅠ,100% for MarshⅢ),while for tTGA,it was 93.3%(88.8% for MarshⅠ,100%for MarshⅢ).All 15 patients with abnormal histology had clinical features related to GSE.MarshⅠandⅢsubjects had more abdominal pain than Marsh 0(P=0.029),and a similar trend was observed for distension and diarrhea.No differences in the percentage of osteopenia were found between MarshⅠandⅢ(P=0.608).Adherence to follow-up was 69.2%.Of 15 GSE patients,66.7%followed a GFD with 80%responding to it. CONCLUSION:GSE in the general population is frequent and clinically relevant,irrespective of histological severity.tTGA is the marker of choice.Mass screening programs are useful in identifying patients who can benefit from GFD and follow-up. | Meritxell Mariné Fernando Fernández-Baares Montserrat Alsina Carme Farré Montserrat Cortijo Rebeca Santaolalla Antonio Salas Margarita Tomàs Elias Abugattas Carme Loras Ingrid Ordás Josep M Viver Maria Esteve | 2009 | World Journal of Gastroenterology2009，15，11： | 0 |
| 15 | Epidemiological, prevention and control updates of yellow fever outbreak in Brazil显示文摘Yellow fever is an acute viral disease endemic to tropical countries, like Brazil, where, since the 1940 s, has no significant documented outbreaks similar to that observed between 2016/2018(2 045 confirmed cases and 677 deaths; caused by the sylvatic form).The principal manipulating factors inciting this change were absence of appropriate vaccination campaigns and increased urbanization & population growth in forest areas, with prevalence of the virus in the species inhabiting of these areas.The 2016/2018 outbreaks exhibited incidence in areas with historically low or no yellow fever virus activity, triggering a surge in recorded deaths-mainly in the Southeastern states of Brazil.The Brazilian government aggressively responded, reforming the countries' prophylactic measures, including vaccine implementation-as of March, 2018, switching from the former double dose regimen of the vaccine, to a single dose protocol, deemed as adequate.Moreover, some states appropriated the fractionated dosage(1/5 of the standard dose), in foresight of potential vaccine shortages.To prevent the uprising of new sylvatic yellow fever cases in Brazil, it's obligatory the development of effective combative plans, including adaptation of prophylactic measures individually(use of repellents, protective clothing etc.), applicable vaccination campaigns in every endemic region, to raise awareness to locals and visitors alike.Notwithstanding these preventative strategies, the persistence of cases and the recent outbreaks in Brazil, highlight the possible ineffectiveness of combative measures.Based on these considerations, the objective of this review was to raise more awareness of the epidemiological impact of the disease in Brazil. | Marli do Carmo Cupertino Rebeca Garcia Andréia Patrícia Gomes Sérgio Oliveira de Paula Nicholas Mayers Rodrigo Siqueira-Batista | 2019 | Asian Pacific Journal of Tropical Medicine2019，12，2： | 0 |
| 16 | Endoscopic response to tumor necrosis factor inhibitors predicts long term benefits in Crohn's disease显示文摘BACKGROUND Identifying predictors of therapeutic response is the cornerstone of personalized medicine.AIM To identify predictors of long-term mucosal healing(MH) in patients with Crohn's disease(CD) treated with tumor necrosis factor α(TNF-α) inhibitors.METHODS Prospective single center study. Consecutive patients with clinically active CD requiring treatment with a TNF-α inhibitor were included. A baseline segmental CD Endoscopic Index of Severity(CDEIS) ≥ 10 in at least one segment or the presence of ulcerations were required for inclusion. Clinical, biological and endoscopic data were obtained at baseline, weeks 14 and 46. Endoscopic response(ER) was defined as a decrease ≥ 50% from baseline CDEIS and MH as partial CDEIS ≤ 5 in all segments.RESULTS Of 62 patients were included. At baseline, median CD Activity Index and CDEIS were 201 and 6.7, respectively with a significant reduction after one year of treatment(53 and 3.0 respectively, P < 0.001). At week 14, 56% of patients achieved ER and 34% MH. At week 46, the corresponding percentages were 52%and 44%. Baseline disease characteristics or biomarkers did not predict MH. A decrease from baseline CDEIS at week 14 of at least 80% was the best predictor of MH at week 46(59% sensitivity and 91% specificity; area under the curve =0.778).CONCLUSION Clinical and biomarker data are not useful predictors of response to TNF-αinhibitors in CD, whereas ER to induction therapy, defined as 80% reduction in global CDEIS, is a robust predictor of long-term MH. Achievement of this endoscopic endpoint may be considered as a therapeutic target for anti-TNF-αtherapy. | Ignacio Alfaro Maria Carme Masamunt Nuria Planell Alicia López-García Jesús Castro Marta Gallego Rebeca Barastegui Angel Giner Alejandro Vara Azucena Salas Elena Ricart Julián Panés Ingrid Ordás | 2019 | World Journal of Gastroenterology2019，25，14： | 0 |
| 17 | Effect of Guazuma Leaves (Guazuma ulmifolia) Inclusion as Protein Source on Growing Calves显示文摘 | Amalia Cabrera Nez Iliana Del Carmen Daniel Rentera Miguel Angel Lammoglia Villagómez César EnrqueMartínez Sánchez Sara Aída Alarcón Pulido Rebeca Rojas-Ronquillo | 2018 | Journal of Agricultural Science and Technology(A)2018，8，1： | 0 |
| 18 | Ten years of lung cancer in a single center: gender, histology, stage and survival显示文摘Aim:The aim was to describe,in a prospective manner,the clinical,histopathological and epidemiological characteristics of lung cancer patients who attended as outpatients at the Lluís Alcanyís,Xàtiva Medical Oncology Hospital,València,Spain from January 2004 to July 2014.We also analyzed survival and compared our data with that reported in the literature.Methods:Clinical and demographic characteristics were analyzed for the entire series and trends were compared by year of diagnosis.Changes in epidemiology were examined and compared.Results:There were 701 patients(91.4%were men,mean age 67.6).Main histology was squamous cell carcinoma(41.5%).Squamous cell carcinoma prevailed in men(45.5%)and adenocarcinoma(ADC)in women(60.3%).The percentage of men with lung cancer and of patients with squamous cell carcinoma was higher than in the reported worldwide data and remained throughout the 10 years period.Mean survival was low,with<10%survivors at 5 years.Stage of disease remained the main prognostic factor for survival.Conclusion:Squamous cell carcinoma continues to be the most frequent histological type in our area.Male and smoking is associated with lung carcinoma while ADC more often occurs in females.Over the time,our epidemiological and histological patterns have not changed,possibly in relation to maintenance of smoking habits. | Regina Gironés Pedro López Rebeca Chulvi Mamen Cañabate Torregrosa M.Dolores | 2015 | Journal of Cancer Metastasis and Treatment2015，1，1： | 0 |
| 19 | “流动的光”展览厅显示文摘1924年建造的巴塞罗那工人合作社现已荒废近30年:风、雨、鸽子从这里进进出出,随着时间的推移,这栋建筑也变得越来越破败不堪。阳光从屋顶的洞口穿透,将光束直射进老旧大堂的中央,让室内的光景更显颓败。然而对于设计师而言,这并不是一场破坏,反而预示了建筑新的可能性。 | Nina Andreatta Jorge Casajús Judith Casas Eline Cooman Inès Martinel Rebeca Lopez Adria Goula 彭佳 | 2018 | 现代装饰2018，0，7： | 0 |
| 20 | The Global Soil Mycobiome consortium dataset for boosting fungal diversity research显示文摘Fungi are highly important biotic components of terrestrial ecosystems,but we still have a very limited understanding about their diversity and distribution.This data article releases a global soil fungal dataset of the Global Soil Mycobiome consortium(GSMc)to boost further research in fungal diversity,biogeography and macroecology.The dataset comprises 722,682 fungal operational taxonomic units(OTUs)derived from PacBio sequencing of full-length ITS and 18S-V9 variable regions from 3200 plots in 108 countries on all continents.The plots are supplied with geographical and edaphic metadata.The OTUs are taxonomically and functionally assigned to guilds and other functional groups.The entire dataset has been corrected by excluding chimeras,index-switch artefacts and potential contamination.The dataset is more inclusive in terms of geographical breadth and phylogenetic diversity of fungi than previously published data.The GSMc dataset is available over the PlutoF repository. | Leho Tedersoo Vladimir Mikryukov Sten Anslan Mohammad Bahram Abdul Nasir Khalid Adriana Corrales Ahto Agan Aída-M.Vasco-Palacios Alessandro Saitta Alexandre Antonelli Andrea C.Rinaldi Annemieke Verbeken Bobby P.Sulistyo Boris Tamgnoue Brendan Furneaux Camila Duarte Ritter Casper Nyamukondiwa Cathy Sharp César Marín D.Q.Dai Daniyal Gohar Dipon Sharmah Elisabeth Machteld Biersma Erin K.Cameron Eske De Crop Eveli Otsing Evgeny A.Davydov Felipe E.Albornoz Francis Q.Brearley Franz Buegger Genevieve Gates Geoffrey Zahn Gregory Bonito Indrek Hiiesalu Inga Hiiesalu Irma Zettur Isabel C.Barrio Jaan Pärn Jacob Heilmann-Clausen Jelena Ankuda John Y.Kupagme Joosep Sarapuu Jose G.Maciá-Vicente Joseph Djeugap Fovo József Geml Juha M.Alatalo Julieta Alvarez-Manjarrez Jutamart Monkai Kadri Põldmaa Kadri Runnel Kalev Adamson Kari A.Bråthen Karin Pritsch Kassim I.Tchan Kęstutis Armolaitis Kevin D.Hyde Kevin K.Newsham Kristel Panksep Lateef A.Adebola Louis J.Lamit Malka Saba Marcela Eda Silva Cáceres Maria Tuomi Marieka Gryzenhout Marijn Bauters Miklós Bálint Nalin Wijayawardene Niloufar Hagh-Doust Nourou S.Yorou Olavi Kurina Peter E.Mortimer Peter Meidl RHenrik Nilsson Rasmus Puusepp Rebeca Casique-Valdés Rein Drenkhan Roberto Garibay-Orijel Roberto Godoy Saleh Alfarraj Saleh Rahimlou Sergei Põlme Sergey V.Dudov Sunil Mundra Talaat Ahmed Tarquin Netherway Terry W.Henkel Tomas Roslin Vladimir E.Fedosov Vladimir G.Onipchenko WAErandi Yasanthika Young Woon Lim Meike Piepenbring Darta Klavina Urmas Kõljalg Kessy Abarenkov | 2021 | Fungal Diversity2021，，6： | 0 |
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