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4篇 您的检索式:作者名="Rebecca McGee"
    题名 作者 年代 出处 被引量
1Possible linkages between lignite aquifers, pathogenic microbes, and renal pelvic cancer in northwestern Louisiana, USA显示文摘Joseph E. Bunnell Calin A. Tatu Rebecca N. Bushon Donald M. Stoeckel Amie M. G. Brady Marisa Beck Harry E. Lerch Benton McGee Bradford C. Hanson Runhua Shi William H. Orem 2006Environmental Geochemistry and Health2006,,6:1
2Genetic diversity, population structure and genome-wide marker-trait association analysis emphasizing seed nutrients of the USDA pea (Pisum sativum L.) core collection显示文摘Soon-Jae Kwon Allan Brown Jinguo Hu Rebecca McGee Chasity Watt Ted Kisha Gail Timmerman-Vaughan Michael Grusak Kevin McPhee Clarice Coyne 2012Genes & Genomics2012,,3:1
3Arctic ground squirrel neuronal progenitor cells resist oxygen and glucose deprivation-induced death显示文摘AIM: To investigate the influence of ischemia/reperfusion on arctic ground squirrel(AGS) neuronal progenitor cells(NPCs), we subjected these cultured cells to oxygen and glucose deprivation.METHODS: AGS NPCs were expanded and differentiated into NPCs and as an ischemia vulnerable control, commercially available human NPCs(hNPCs) were seeded from thawed NPCs. NPCs, identified by expression of TUJ1 were seen at 14-21 d in vitro(DIV). Cultures were exposed to control conditions, hypoxia, oxygen and glucose deprivation or glucose deprivation alone or following return to normal conditions to model reperfusion. Cell viability and death were assessed from loss of ATP as well as from measures of alamarB lue~ and lactate dehydrogenase in the media and from counts of TUJ1 positive cells using immunocytochemistry. Dividing cells were identified by expression of Ki67 and phenotyped by double labeling with GFAP, MAP2 ab or TUJ1. RESULTS: We report that when cultured in NeuraLife^(TM), AGS cells remain viable out to 21 DIV, continue to express TUJ1 and begin to express MAP2 ab. Viability of hN PCs assessed by fluorescence alamarB lue(arbitrary units) depends on both glucose and oxygen availability [viability of hNPCs after 24 h oxygen glucose deprivation(OGD) with return of oxygen and glucose decreased from 48151 ± 4551 in control cultures to 43481 ± 2413 after OGD, P < 0.05]. By contrast, when AGS NPCs are exposed to the same OGD with reperfusion at 14 DIV, cell viability assessed by alamar Blue increased from 165305 ± 11719 in control cultures to 196054 ± 13977 after OGD. Likewise AGS NPCs recovered ATP(92766 ± 6089 in control and 92907 ± 4290 after modeled reperfusion; arbitrary luminescence units), and doubled in the ratio of TUJ1 expressing neurons to total dividing cells(0.11 ± 0.04 in control cultures vs 0.22 ± 0.2 after modeled reperfusion, P < 0.05). Maintaining AGS NPCs for a longer time in culture lowered resistance to injury, however, did not impair proliferation of NPCs relative to other cell lineages after oxygen deprivation followed by re-oxygenation.CONCLUSION: Ischemic-like insults decrease viability and increase cell death in cultures of human NPCs. Similar conditions have less affect on cell death and promote proliferation in AGS NPCs.Kelly L Drew Matthew Wells Rebecca McGee Austin P Ross Judith Kelleher-Andersson 2016World Journal of Biological Chemistry2016,7,1:1
4Exercise increases nuclear AMPK or2 in human skeletal muscle 显示文摘SEAN L MCGEE KIRSTEN H REBECCA L S et aI 2003Diabetes2003,52,4:1
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