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| 1 | Enhanced expression of interleukin-18 in serum and pancreas of patients with chronic pancreatitis显示文摘AIM: To investigate interleukin-18 (IL-18) in patients with chronic panreatitis (CP). METHODS: We studied 29 patients with CP and 30 healthy controls. Peripheral blood mononuclear cells (PBMC) were isolated and incubated with 50 mmol/L ethanol, lipopolysaccharide (LPS) (doses 25 g/L, 250 g/L, 2500 g/L) and both agents for 24 h. Levels of IL-18 in the supernatants, and levels of IL-18, IL-12, interferon (IFN)-γ and soluble CD14 in the serum were analysed by ELISA technique. Expression of IL-18 in PBMC was investigated by reverse-transcription (RT)-PCR. IL-18 protein levels in CP tissue and in normal pancreas were studied by ELISA technique. IL-18 levels in PBMC and pancreatic tissue were determined by Westernblot. Im-munohistochemistry for pancreatic IL-18 expression was performed. RESULTS: In patients, IL-18 serum levels were signifi-cantly enhanced by 76% (mean: 289.9 ± 167.7 ng/L) compared with controls (mean: 165.2 ± 43.6 ng/L; P < 0.0005). IL-12 levels were enhanced by 25% in patients (18.3 ± 7.3 ng/L) compared with controls (14.7 ± 6.8ng/L, P = 0.0576) although not reaching the statistical significance. IFN-γ and soluble CD14 levels were not in-creased. In vitro, LPS stimulated significantly and dose-dependently IL-18 secretion from PBMC. Incubation with ethanol reduced LPS-stimulated IL-18 secretion by about 50%. The mRNA expression of IL-18 in PBMC and the response of PBMC to ethanol and LPS was similar in CP patients and controls. In PBMC, no significant differences in IL-18 protein levels were detected between patients and controls. IL-18 protein levels were increased in CP tissues compared to normal pancreatic tissues. IL-18 was expressed by pancreatic acinar cells and by infiltrating inflammatory cells within the pancreas. CONCLUSION: IL-18 originates from the chronically inflammed pancreas and appears to be involved in the fibrotic destruction of the organ. | Alexander Schneider Stephan L Haas Ralf Hildenbrand Sren Siegmund Iris Reinhard Helmut Nakovics Manfred V Singer Peter Feick | 2006 | World Journal of Gastroenterology2006，12，40： | 4 |
| 2 | Deterministic aperiodic arrays of metal nanoparticles for surface-enhanced Raman scattering(SERS)显示文摘 | Gopinath A Boriskina S V Reinhard B M | 2009 | Opt Express2009，17，5： | 1 |
| 3 | Provenance of Cretaceous synorogenic sandstones in the Eastern Alps: constraints from framework petrography, heavy mineral analysis and mineral chemistry显示文摘 | Eynatten H V Reinhard G | 1999 | Sedimentary Geology1999，124，1： | 1 |
| 4 | Olfaction: scent-triggered navigation in honeybees显示文摘 | Reinhard J Srinivasan M V Zhang S | 2004 | Nature2004，427，6973： | 1 |
| 5 | Effects of hypoehlornus acid exposure on the rejection of salt, polyethylene glycols, boron and arsenic (Ⅴ) by nanofiltration and reverse osmosis membranes 显示文摘 | Do V T Tang C Y Reinhard M et at | 2012 | Water Research2012，46，16： | 1 |
| 6 | Scent-triggered Navigation in Honeybees显示文摘 | Reinhard J SrinivasanM V Zbang S | 2004 | Nature2004，427，： | 1 |
| 7 | Structural stability of con- crete wind turbines and solar chimney towers exposed to dynamic wind action 显示文摘 | Reinhard H Gideon P V Z | 2007 | Journal of Wind Engineering2007，95，： | 1 |
| 8 | Hydrodehalogenation of 1- to 3-carbon Halogenated Organic Compounds in Water Using a Palladium Catalyst and Hydrogen Gas 显示文摘 | Lowery G V Reinhard M | 1999 | Environ Sci Technol1999，33，11： | 1 |
| 9 | Scent-triggered navigation in honeybees 显示文摘 | Reinhard J Srinivasan M V Zhang S | 2004 | Nature2004，427，： | 1 |
| 10 | High survival in adult patients with acute respiratory distress syndrome treated by extracorporeal membrane oxygenation, minimal sedation, and pressure supported ventilation显示文摘 | Linden V Palmer K Reinhard J | 2000 | Intensive Care Med2000，26，11： | 1 |
| 11 | Scent-triggered navigation in honeybees显示文摘 | REINHARD J SRIVIVASAN M V ZHANG S | 2004 | Nature2004，427，： | 1 |
| 12 | Theoretical analysis of an artificial superposition compound eye for application in ultra flat digital image acquisition devices显示文摘 | Peter S Reinhard V | 2004 | Pme of SPIE2004，5249，： | 1 |
| 13 | Comparing the Vancouver Scar Scale with the cutometer in the assessment of donor site wounds treated with various dressings in a randomized trial显示文摘 | Rennekampff H O Rabbels J Reinhard V | 2006 | J Burn Care Res2006，27，3： | 1 |
| 14 | Antigen retrieval,signal amplification and intensification in immunohistochemistry显示文摘 | MARTIN W REINHARD V W PAUL K | 1996 | Histochem Cell Biol1996，105，4： | 1 |
| 15 | Monosaccharide-linked inhibitors of O6-methylguanine-DNAmethyltransferase (MGMT):synthesis,molecular modeling,and structure-activity relationships显示文摘 | Reinhard J H u l l W E v o n der Lieth C W e t al | 2001 | J Med C hem2001，44，24： | 1 |
| 16 | On reliability estimation based on fuzzy lifetime data 显示文摘 | Reinhard V | 2009 | Journal of Statistical Planning and Inference2009，139，5： | 1 |
| 17 | First GOCE gravity field models derived by three different approaches显示文摘 | Roland P Sean B Federica M Christoph F Helmut G Wolf D S Eduand H Mirko R Jan M B Oleg A Mantin V Thomas F Reinhard M Ina K Fernando S and Carl C T | | 0，，11： | 1 |
| 18 | High survival in adult patients with acute respiratory distress syndrome treated by extracorporeal mem brane oxygenation, minimal sedation, and pressure supported wentilation显示文摘 | Palmer K Reinhard J | 2000 | Intensive Care Med2000，26，： | 1 |
| 19 | Tumor necrosis factor 2-induced activation of c-jun N-terminal kinase is mediated by TRAF2显示文摘 | REINHARD C SHAMOON B SHYAMALA V | 1997 | EMBO J1997，16，5： | 1 |
| 20 | Provenance of Cretaceous synorogenic sandstones in the Eastern Alps: constraints from framework petrography, heavy mineral analysis and mineral chemistry显示文摘 | HILMAR V E REINHARD G | 1999 | Sedimentary Geology1999，124，4： | 1 |
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