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| 1 | Lower Bifidobacteria counts in both duodenal mucosa-associated and fecal microbiota in irritable bowel syndrome patients显示文摘AIM: To determine the composition of both fecal and duodenal mucosa-associated microbiota in irritable bowel syndrome (IBS) patients and healthy subjects using molecular-based techniques. METHODS: Fecal and duodenal mucosa brush samples were obtained from 41 IBS patients and 26 healthy subjects. Fecal samples were analyzed for the composition of the total microbiota using fluorescent in situ hybridization (FISH) and both fecal and duodenal brush samples were analyzed for the composition of bif idobacteria using real-time polymerase chain reaction. RESULTS: The FISH analysis of fecal samples revealed a 2-fold decrease in the level of bifidobacteria (4.2 ± 1.3 vs 8.3 ± 1.9, P < 0.01) in IBS patients compared to healthy subjects, whereas no major differences in other bacterial groups were observed. At the species level, Bifidobacterium catenulatum levels were significantly lower (6 ± 0.6 vs 19 ± 2.5, P < 0.001) in the IBS patients in both fecal and duodenal brush samples than in healthy subjects.CONCLUSION: Decreased bifidobacteria levels in both fecal and duodenal brush samples of IBS patients compared to healthy subjects indicate a role for microbiotic composition in IBS pathophysiology. | Angèle PM Kerckhoffs Melvin Samsom Michel E van der Rest Joris de Vogel Jan Knol Kaouther Ben-Amor Louis MA Akkermans | 2009 | World Journal of Gastroenterology2009，15，23： | 33 |
| 2 | A heat shock following electroporation induces highly efficient transformation of Corynebacterium glutamicum with xenogeneic plasmid DNA 显示文摘 | M E vander Rest C Lange D Molenaar | 1999 | Applied Microbiology and Biotechnology1999，52，4： | 1 |
| 3 | Endometrial mi-cropolyps at fluid hysteroscopy suggest the existence of chro-nic endometritis 显示文摘 | Cicinelli E Rests L Nicoletti R | 2005 | Hum Repred2005，20，5： | 1 |
| 4 | A heat shock following electroporation induces highly efficient transformation of Corynebacterium glutamicum with xenogeneic plasmid DNA显示文摘 | van der Rest M E Lange C Molenaar D | 1999 | Appl Microbiol Biotechnol1999，52，： | 1 |
| 5 | Incidence,risk factors,and outcome of ventilator associated pneumonia显示文摘 | Tejerina E Frutos Vivar F Rest repo M I | 2006 | J Crit Ca re2006，21，1： | 1 |
| 6 | Endometrial micropolyps at fluid hysteroscopy suggest the existence of chronic endometfitis显示文摘 | Cieinelli E Rests LI Nicoletti R | 2005 | Hum Repred2005，20，5： | 1 |
| 7 | A heat shock following electroporation induces highly efficient trans- formation of Corynebacterium glutamicum with xenoge- netic plasmid DNA显示文摘 | van der Rest M E Lange C Molenaar D | 1999 | Applied Microbiology and Bio- technology1999，52，4： | 1 |
| 8 | Endometrial mi-cropolyps at fluid hysteroscopy suggest the existence of chro-nic endometritis 显示文摘 | Cicinelli E Rests L Nicoletti R | 2005 | Hum Repred2005，20，5： | 1 |
| 9 | Life cycles, dynamics, exploita- tion and management of coastal Penaeid shrimp stocks 显示文摘 | Caycia S Reste L E | 1981 | FAO Fish Tech1981，，203： | 1 |
| 10 | A heat shock following electreporation induces highly efficient transformation of Corynebacteriurn glutamicum with xenogeneic plasmid DNA 显示文摘 | Van der Rest M E Lange C Molenaar D | 1999 | Appl Microbiol Biotechnol1999，52，： | 1 |
| 11 | Glycerophosphoeholine metabolism in higher plant cells 显示文摘 | Van der Rest B Boisson A Gout E | 2002 | Plant Physiology2002，30，1： | 1 |
| 12 | Tetradecylselenoacetic acid, a PPAR ligand with antioxidant, antiinflammatory, and hypolipidemic properties显示文摘 | Dyroy E Rest TH Pettersen R J | 2007 | Arterioscler Thromb Vasc Biol2007，27，3： | 1 |
| 13 | Characterization of Bacillus anthracis arginase:effects of pH,temperature,and cell viability on metal preference显示文摘 | Viator R J Rest R F Hildebrandt E | | 0，，： | 1 |
| 14 | Characterization of Bacillus anthracis arginase: effects of pH, temperature, and cell viability on metal preference显示文摘 | Viator R J Rest R F Hildebrandt E | 2008 | BMC Biochem2008，9，： | 1 |
| 15 | Exhaled Interleukine-6 and 8-isoprostane in chronic obstructive pulmonary disease:effect of earbecysteine lysine salt monohydrate(SCMC-Lys)显示文摘 | Carpagnano G E Rests O Foschion-Barbaro M P | 2004 | Eur J Pharmacol2004，505，13： | 1 |
| 16 | The ternary system Au - Fe - Sn 显示文摘 | Alexandra Neumann Kjekshus A Rest E | 1996 | Journal of Alloys and Compounds1996，238，12： | 1 |
| 17 | The ternary system Au - Ni - Sn 显示文摘 | Alexandra Neumann Kjekshus A Rest E | 1996 | Journal of Solid State Chemistry1996，123，2： | 1 |
| 18 | A heat shock following electroporation induces highly efficient transformation of Corynebacterium glutamicum with xenogeneic plasmid DNA显示文摘 | Lange C Molenaar D | 1999 | Appl Microbiol Biotechnol1999，52，： | 1 |
| 19 | A heat shock following electroporation induces highly efficient transformation of Corvnebacterium glutamicum with xenogenetic plasmid DNA显示文摘 | Van Der Rest M E Lange C Molenaar D | 1999 | Applied Microbiology and Biotechnology1999，52，： | 1 |
| 20 | A heat shock following electroporation induces highly efficient transformation of Corynebacterium glutamicure with xenogenetic plasmid DNA 显示文摘 | van der REST M E LANGE C MOLENAAR D | 1999 | Appl Microbio Biotech1999，52，： | 1 |
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