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| 1 | AZF microdeletions associated with idiopathic and non idiopathic cases with cryptorchidism and varicocele显示文摘Aim:To identify submicroscopic interstitital deletions in azoospermia factor (AZF) loci in idiopathic and non-idiopathic cases of male infertility in Indians.Methos:One hundred and twenty two infertile males with oligozoospermia or azoospermia were included in this study.Semen analysis was done to determine the sperm density,i.e.,normospermia(>20million/mL),oligozoospermia(<20million/mL) or azoospermia.They were subjected to detailed clinical examination and endocrinological and cytogenetic study.Thirty G-banded metaphases were analyzed in the 122 cases and polymerase chain reaction (PCR) microdeletion analysis was done in 70 cytogenetically normal subjects.For this genomic DNA was extracted using peripheral blood.The STS primers tested in each case were sY84,sY86(AZFa);sY127,sY134(AZFb);sY254,sY255(AZFc).PCR amplifications found to be negative were repeated at least 3 times to confirm the deletion of a given marker.The PCR products were analyzed on a 1.8% agarose gel.Results:Eight of the 70 cades(11.4%) showed deletion of at least one of the STS markers.Deletions were detected in cases with known and unknown aetiology with bilateral severe testiculopathy and also in cryptorchid and varicocele subjects.Conclusion.AZF microdeletions were seen in both idiopathic and non-idiopathic cases with cryptorchidism and varicocele.The finding of a genetic aetiology in infertile men with varicocele and cryptorchidism suggests the need for molecular screening in non-idiopathic cases. | Rima Dada N.P. Gupta K. Kucheria | 2002 | Asian Journal of Andrology2002，4，4： | 22 |
| 2 | Y chromosome microdeletions in azoospermic patients with Klinefelter's syndrome显示文摘瞄准:与 Klinefelter 的症候群(KFS ) 在 azoospermic 病人学习 Y 微删除的出现。方法:血和精液样品与 KFS 从 azoospermic 病人被收集(n = 14 ) 并且证明富饶的人的一个控制组(n = 13 ) 。精液分析根据世界卫生组织被做() 指南。血样品为 karyotyping,荧光灯的原位杂交(鱼) 和由放射性免疫测定刺激荷尔蒙(FSH ) 的血浆滤泡的测量被处理。决定 Y 微删除, 16 顺序的聚合酶链反应(PCR ) 标注了地点(圣) ,三基因(DFFRY, XKRY 和 RBM1Y ) 在孤立的 genomic DNA 上被执行。阴囊的好针渴望细胞学(FNAC ) 在选择情况中被做。结果:跨越一个 and AZFb 部位与 KFS 在 14 个 azoospermic 病人中的四个被发现的精子缺乏因素(AZF ) 的 Y 微删除。染色质组型和鱼分析表明显示出 Y 微删除的四个盒子,三个盒子有一 47, XXY/46, XY 染色体的模式和一个盒子有一 46, XY/47, XXY/48, XXXY/48, XXYY 染色体的模式。有 Y 微删除的一件样品的阴囊的 FNAC 揭示了形态学的 Sertoli 房间唯一的类型。然而,没有 Y 微删除在任何 13 个肥沃的人被观察。有 KFS 的所有病人提高了血浆 FSH 层次。结论:有 KFS 的病人可以怀有 Y 微删除并且为这些屏蔽应该是他们的诊断病情的检查的部分,特别地在那些考虑帮助了繁殖技术。 | Anurag Mitra Rima Dada Rajeev Kumar Narmada Prasad Gupta Kiran Kucheria Satish Kumar Gupta | 2006 | Asian Journal of Andrology2006，8，1： | 8 |
| 3 | Higher frequency of Yq microdeletions in sperm DNA as compared to DNA isolated from blood显示文摘瞄准:决定删除的 Yq 微删除频率和 loci 是否在不同胚胎学的起源的二纸巾(血和精子) 是类似的。方法:现在的学习包括了 52 个不肥沃的 oligozoospermic 盒子。在每种情况中, DNA 从血和精子被孤立,微删除分析从 genomic DNA 被做的聚合酶链反应(PCR ) 从两个孤立纸巾。PCR 产品在 1.8% 统帅玫瑰胶化上被分析。发现否定的 PCR 扩大被重复至少三次证实一个给定的标记的删除。结果:仅仅 1 个盒子在血 DNA 怀有微删除,而 4 个盒子在精子 DNA 怀有微删除。结论:Yq 微删除的频率作为与血相比在细菌房间是更高的。为帮助繁殖生产技术(艺术) 作为不肥沃的夫妇的多数抉择, Yq 微删除从细菌房间屏蔽是重要的理解不孕的基因基础,提供全面建议和大多数改编治疗学给不肥沃的夫妇。 | Rima Dada Rakesh Kumar M. B. Shamsi Rajeev Kumar Kiran Kucheria Raj K. Sharma Satish K. Gupta Narmada P. Gupta | 2007 | Asian Journal of Andrology2007，9，5： | 6 |
| 4 | Mild oxidative stress is beneficial for sperm telomere length maintenance显示文摘AIM:To evaluate telomere length in sperm DNA and its correlation with oxidative stress(normal,mild,severe).METHODS:The study included infertile men(n=112)and age matched fertile controls(n=102).The average telomere length from the sperm DNA was measured using a quantitative real time PCR based assay.Seminal reactive oxygen species(ROS)and 8-Isoprostane(8-IP)levels were measured by chemiluminescence assay and ELISA respectively.RESULTS:Average sperm telomere length in infertile men and controls was 0.609±0.15 and 0.789±0.060,respectively(P<0.0001).Seminal ROS levels in infertile was higher[66.61±28.32 relative light units(RLU)/s/million sperm]than in controls(14.04±10.67RLU/s/million sperm)(P<0.0001).The 8-IP level in infertile men was significantly higher(421.55±131.29pg/mL)than in controls(275.94±48.13 pg/mL)(P<0.001).When correlated to oxidative stress,in normal range of oxidative stress(ROS,0-21.3 RLU/s/million sperm)the average telomere length in cases was 0.663±0.14,in mild oxidative stress(ROS,21.3-35 RLU/s/million sperm)it was elevated(0.684±0.12)and in severe oxidative stress(ROS>35 RLU/s/million sperm)average telomere length was decreased to 0.595±0.15.CONCLUSION:Mild oxidative stress results in lengthening of telomere length,but severe oxidative stress results in shorter telomeres.Although telomere maintenance is a complex trait,the study shows that mild oxidative stress is beneficial in telomere length maintenance and thus a delicate balance needs to be established to maximize the beneficial effects of free radicals and prevent harmful effects of supra physiological levels.Detailed molecular evaluation of telomere structure,its correlation with oxidative stress would aid in elucidating the cause of accelerated telomere length attrition. | Swetasmita Mishra Rajeev Kumar Neena Malhotra Neeta Singh Rima Dada | 2016 | World Journal of Methodology2016，6，2： | 4 |
| 5 | Molecular screening for Yq microdeletion in men with idiopathic oligozoospermia and azoospermia显示文摘 | Rima Dada N. P. Gupta K. KucherIA | 2003 | Journal of Biosciences2003，，2： | 1 |
| 6 | Role of Genetics in Azoospermia显示文摘 | Joo Yeon Lee Rima Dada Edmund Sabanegh Angelo Carpi | | 0，，： | 1 |
| 7 | Molecular screening for Yq microdeletion in men with idiopathic oligozoospermia and azoospermia显示文摘 | Rima Dada N. P. Gupta K. KucherIA | 2003 | Journal of Biosciences2003，，2： | 1 |
| 8 | Potential Markers for Detection and Monitoring of Ovarian Cancer显示文摘 | Brandon J. D. Rein Sajal Gupta Rima Dada Joelle Safi Chad Michener Ashok Agarwal Nelson N. H. Teng | 2011 | Journal of Oncology2011，，： | 1 |
| 9 | At- tenuation of oxidative stress & dna damage in varicocelecto- my: implications in infertility management 显示文摘 | Rima Dada Monis Bilal Shamsi Sunderarjan Venkatesh | 2010 | Indian jour nal of medical research2010，132，6： | 1 |
| 10 | Ocular manifestations of central insulin resistance显示文摘Central insulin resistance, the diminished cellular sensitivity to insulin in the brain, has been implicated in diabetes mellitus, Alzheimer’s disease and other neurological disorders. However, whether and how central insulin resistance plays a role in the eye remains unclear. Here, we performed intracerebroventricular injection of S961, a potent and specific blocker of insulin receptor in adult Wistar rats to test if central insulin resistance leads to pathological changes in ocular structures. 80 mg of S961 was stereotaxically injected into the lateral ventricle of the experimental group twice at 7 days apart, whereas buffer solution was injected to the sham control group. Blood samples, intraocular pressure, trabecular meshwork morphology, ciliary body markers, retinal and optic nerve integrity, and whole genome expression patterns were then evaluated. While neither blood glucose nor serum insulin level was significantly altered in the experimental or control group, we found that injection of S961 but not buffer solution significantly increased intraocular pressure at 14 and 24 days after first injection, along with reduced porosity and aquaporin 4 expression in the trabecular meshwork, and increased tumor necrosis factor α and aquaporin 4 expression in the ciliary body. In the retina, cell density and insulin receptor expression decreased in the retinal ganglion cell layer upon S961 injection. Fundus photography revealed peripapillary atrophy with vascular dysregulation in the experimental group. These retinal changes were accompanied by upregulation of pro-inflammatory and pro-apoptotic genes, downregulation of anti-inflammatory, anti-apoptotic, and neurotrophic genes, as well as dysregulation of genes involved in insulin signaling. Optic nerve histology indicated microglial activation and changes in the expression of glial fibrillary acidic protein, tumor necrosis factor α, and aquaporin 4. Molecular pathway architecture of the retina revealed the three most significant pathways involved being inflammation/cell stress, insulin signaling, and extracellular matrix regulation relevant to neurodegeneration. There was also a multimodal crosstalk between insulin signaling derangement and inflammation-related genes. Taken together, our results indicate that blocking insulin receptor signaling in the central nervous system can lead to trabecular meshwork and ciliary body dysfunction, intraocular pressure elevation, as well as inflammation, glial activation, and apoptosis in the retina and optic nerve. Given that central insulin resistance my lead to neurodegenerative phenotype in the visual system, targeting insulin signaling may hold promise for vision disorders involving the retina and optic nerve. | Muneeb A.Faiq Trina Sengupta Madhu Nath Thirumurthy Velpandian Daman Saluja Rima Dada Tanuj Dada Kevin C.Chan | 2023 | Neural Regeneration Research2023，18，5： | 0 |
| 11 | Implications of Cytogenetic Abnormalities and Azoospermia Factor Microdeletions in Assisted Procreation显示文摘Assisted procreation techniques have revolutionized the management of infertility and have offered hope to millions of infertile couples. The main aim of these proce- dures is to produce healthy offspring. However recent studies on short term outcome of ART have reported a higher incidence of low birth weight, development delay, imprinting defects, sex and autosomal structural abnormalities, major and minor congenital malformation and certain cancers in babies conceived via ART. Further the health of ART conceived children beyond the neonatal period have been less well evaluated. A large number of infertile couples opting for ART have an underlying genetic aetiology. These genetic aberrations are iatrogenitically transmitted via ART. Thus it is important that all couples undergo a detailed and comprehensive genetic evaluation prior to ART. | Rima Dada R Kumar M Bilal Shamsi Sarabpreet Singh Col R K Sharma A Varshney | 2007 | Journal of Reproduction and Contraception2007，18，3： | 0 |
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