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| 1 | Induction of apoptosis on human hepatocarcinoma cell lines by an alkyl resorcinol isolated from Lithraea molleoides显示文摘AIM: To study the mechanism of cytotoxicity of a new active 5-alkyl resorcinol [1, 3-dihydroxy-5- (tridec-4’, 7’ -dienyl) benzene] isolated from Lithraea molleoides leaves on liver tumor cells. METHODS: Human hepatocarcinoma cell lines (HepG2 and Hep3B) in culture were treated with inhibitory concentrations, 50% of the compound, for 24 h. The induction of apoptosis was detected in treated cells by analysis of DNA fragmentation, DNA content, and acridine orange and propidium iodide staining. RESULTS: After 24 h of 5-alkyl resorcinol treatment, both cell lines showed: (1) the typical morphological alterations of apoptosis; (2) DNA fragmentation, detected by laddering and appearance of a subG0 population by flow cytometry; and (3) condensed and fragmented nuclei by acridine orange-propidium iodide staining. CONCLUSION: Based on the results, this compound exerts its cytotoxic effect in both hepatocellular cell lines through apoptotic cell death. For Hep3B, cells with mutated p53 and Fas, apoptosis would proceed by p53- or Fas-independent pathways. | Luciana Barbini Paula Lopez Julieta Ruffa Virginia Martino Graciela Ferraro Rodolfo Campos Lucia Cavallaro | 2006 | World Journal of Gastroenterology2006，12，37： | 3 |
| 2 | Acute hepatitis C in a chronically HIV-infected patient:Evolution of different viral genomic regions显示文摘AIM: To analyze the molecular evolution of different viral genomic regions of HCV in an acute HCV infected patient chronically infected with HIV through a 42-month follow-up.METHODS: Serum samples of a chronically HIV infected patient that seroconverted to anti HCV antibodies were sequenced, from the event of superinfection through a period of 17 months and in a late sample (42nd month). Hypervariable genomic regions of HIV (V3 loop of the gp120) and HCV (HVR-1 on the E2 glycoprotein gene) were studied. In order to analyze genomic regions involved in different biological functions and with the cellular immune response, HCV core and NS5A were also chosen to be sequenced. Amplification of the different regions was done by RT-PCR and directly sequenced. Confirmation of sequences was done on reamplified material. Nucleotide sequences of the different time points were aligned with CLUSTAL W 1.5, and the corresponding amino acid ones were deduced.RESULTS: Hypervariable genomic regions of both viruses (HVR1 and gp120 V3 loop) presented several nonsynonymous changes but, while in the gp120 V3 loop mutations were detected in the sample obtained right after HCV superinfection and maintained throughout, they occurred following a sequential and cumulative pattern in the HVR1. In the NS5A region of HCV, two amino acid changes were detected during the follow-up period, whereas the core region presented several amino acid replacements, once the HCV chronic infection had been established.CONCLUSION: During the HIV-HCV superinfection, each genomic region analyzed shows a different evolutionary pattem.Most of the nucleotide substitutions observed are nonsynonymous and clustered in previously described epitopes,thus suggesting an immune-driven evolutionary process. | Diego Flichman Veronica Kott Silvia Sookoian Rodolfo Campos | 2003 | World Journal of Gastroenterology2003，9，7： | 2 |
| 3 | Molecular epidemiology and genetic diversity of hepatitis B virus in Mar del Plata city, Argentina显示文摘 | Luciana Barbini Mercedes Elizalde Carolina Torres Rodolfo Campos | 2013 | Infection, Genetics and Evolution2013，，： | 1 |
| 4 | New perspectives on the evolutionary history of hepatitis B virus genotype F显示文摘 | Carolina Torres Flavia Guadalupe Pi?eiro y Leone Silvana Claudia Pezzano Viviana Andrea Mbayed Rodolfo Héctor Campos | 2011 | Molecular Phylogenetics and Evolution2011，，1： | 1 |
| 5 | Antiviral activity of 5′- O -carbonate-2′,3′-dideoxy-3′-thiacytidine prodrugs against hepatitis B virus in HepG2 2.2.15 cells显示文摘 | Dolores Gagey Soledad Ravetti Eliana F. Castro María Soledad Gualdesi Margarita C. Bri?on Rodolfo H. Campos Lucía V. Cavallaro | 2010 | International Journal of Antimicrobial Agents2010，，6： | 1 |
| 6 | Hepatitis B precore/core promoter mutations in isolates from HBV-monoinfected and HBV–HIV coinfected patients: A 3-yr prospective study显示文摘 | Lucila Cassino Natalia Laufer Horacio Salomon Rodolfo Campos Jorge Quarleri | 2009 | Journal of Clinical Virology2009，，4： | 1 |
| 7 | Dynamic changes in viral population structure and compartmentalization during chronic hepatitis C virus infection in children显示文摘 | María Inés Gismondi Juan María Díaz Carrasco Pamela Valva Pablo Daniel Becker Carlos Alberto Guzmán Rodolfo Héctor Campos María Victoria Preciado | 2013 | Virology (-)2013，，1： | 1 |
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