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| 1 | Programmed Self-Elimination of the CRISPR/Cas9 Construct Greatly Accelerates the Isolation of Edited and Transgene-Free Rice Plants显示文摘 | Yubing He Min Zhu Lihao Wang Junhua Wu Qiaoyan Wang Rongchen Wang Yunde Zhao | 2018 | Molecular Plant2018，11，9： | 18 |
| 2 | Self-cleaving ribozymes enable the production of guide RNAs from unlimited choices of promoters for CRISPR/Cas9 mediated genome editing显示文摘Development of tools for targeted modifications of specific DNA sequences in plants is of great importance to basic plant biology research as well as crop improvement.The ability to cut DNA at specific locations in the genome to generate doublestrand breaks(DSBs)in vivo is a prerequisite for any genome editing efforts.Several engineered nucleases including zinc finger nucleases(ZFNs)and transcriptional activator-like nucleases(TALENs)have been successfully used to edit genomes.However, | Yubing He Tao Zhang Ning Yang Meilian Xu Lang Yan Lihao Wang Rongchen Wang Yunde Zhao | 2017 | Journal of Genetics and Genomics2017，44，9： | 14 |
| 3 | Genomic analysis of a nutrient response in Arabidopsis reveals diverse expression patterns and novel metabolic and potential regulatory genes induced by nitrate显示文摘 | Rongchen Wang Karl Guegler Samuel T Labrie | 2000 | Plant Cell2000，12，8： | 1 |
| 4 | Positional effects on efficiency of CRISPR/Cas9-based transcriptional activation in rice plants显示文摘The nuclease-dead Cas9(dCas9)has been reprogrammed for transcriptional activation by fusing dCas9 to a transcriptional activation domain.In the presence of a guide RNA(gRNA),the dCas9 fusions specifically bind to regions of a promoter to activate transcription.Significant amount of effort has been directed toward the identification and optimization of the fusions of dCas9-activation domain,but very little is known about the impact of gRNA target positions within a promoter in plants on transcriptional activation efficiency.The dCas9–6TAL–VP128 system(dCas9-TV)has been optimized to activate transcription in plants.Here we use the dCas9-TV to activate transcription of OsWOX11 and OsYUC1,two genes that cause dramatic developmental phenotypes when overexpressed.We designed a series of gRNAs targeting the promoters of the two genes.We show that gRNAs that target regions within 350 bp upstream of the transcription start site were most effective in transcriptional activation.Moreover,we show that using two gRNAs that simultaneously target two discrete sites in a promoter can further enhance transcription.This work provides guidelines for designed transcriptional activation through CRISPR/dCas9 systems. | Xiaoyu Gong Tao Zhang Jialing Xing Rongchen Wang Yunde Zhao | 2020 | aBIOTECH2020，1，1： | 1 |
| 5 | Hydrogen Sulfide-Specific and NIR-Light-Controllable Synergistic Activation of Fluorescent Theranostic Prodrugs for Imaging-Guided Chemo- Photothermal Cancer Therapy显示文摘Theranostic prodrugs are promising for cancer medicine;however,the inability to activate these systems exclusively at the desired tumor location compromises the specificity and efficacy of cancer treatment.Here,we developed a fluorescent theranostic nanoprodrug with synergistic hydrogen-sulfidespecific and near-infrared(NIR)-light-controllable activation for imaging-guided chemo-photothermal cancer therapy.This nanoprodrug system was fabricated by the inclusion of hydrogen sulfide(H2S)-activatable small molecule to the theranostic prodrug and a photothermal transducer in the interior of a NIR-light-responsive container. | Ge Xu Wei Guo Xianfeng Gu Zhijun Wang Rongchen Wang Tianli Zhu He Tian Chunchang Zhao | 2020 | CCS Chemistry2020，2，4： | 1 |
| 6 | Synergistic roles of LAX1 and FZP in the development of rice sterile lemma显示文摘Rice florets are subtended by two sterile lemmas,whose origin and biological functions have not been studied extensively.Here we demonstrate that two putative transcription factors,LAX PANICLE1(LAX1)and FRIZZY PANICLE(FZP),synergistically control the development of sterile lemmas.Both LAX1 and FZP are previously known for their roles in panicle and floret development.Disruption of either LAX1 or FZP greatly reduces the number of floret development.We generated new lax1 mutants(lax1-c)using CRISPR/Cas9 gene editing technology.In addition to the expected lax panicle phenotypes,we noticed that a significant number of spikelets of lax1-c developed elongated sterile lemmas.Moreover,our characterization of lax1-RNAi plants also revealed sterile lemma phenotypes similar to lax1-c mutants.We isolated a weak allele of fzp(fzp-14)in a genetic screen for lax1–1 enhancers.The fzp-14 lax1–1 double mutants completely eliminated flower development.Interestingly,the isolated fzp-14 produced spikelets with elongated sterile lemmas.Furthermore,fzp-14 was haploid-insufficient in the lax1–1 background whereas fzp-14 heterozygous plants were indistinguishable from wild type plants.The lax1–1 fzp-14+/−also developed elongated sterile lemma as observed in lax1-c,lax1-RNAi,and fzp-14,suggesting that LAX1 and FZP synergistically control sterile lemma development. | Yidong Wang Shanshan Wei Yubing He Lang Yan Rongchen Wang Yunde Zhao | 2020 | The Crop Journal2020，8，1： | 1 |
| 7 | Improvements of TKC Technology Accelerate Isolation of Transgene-Free CRISPR/Cas9-Edited Rice Plants显示文摘Elimination of the CRISPR/Cas9 constructs in edited plants is a prerequisite for assessing genetic stability, conducting phenotypic characterization, and applying for commercialization of the plants. However, removal of the CRISPR/Cas9 transgenes by genetic segregation and by backcross is laborious and time consuming. We previously reported the development of the transgene killer CRISPR(TKC) technology that uses a pair of suicide genes to trigger self-elimination of the transgenes without compromising gene editing efficiency. The TKC technology enables isolation of transgene-free CRISPR-edited plants within a single generation, greatly accelerating crop improvements. Here, we presented two new TKC vectors that show great efficiency in both editing the target gene and in undergoing self-elimination of the transgenes. The new vectors replaced the CaMV35 S promoter used in our previous TKC vector with two rice promoters to drive one of the suicide genes, providing advantages over our previous TKC vector under certain conditions. The vectors reported here offered more options and flexibility to conduct gene editing experiments in rice. | HE Yubing ZHU Min WANG Lihao WU Junhua WANG Qiaoyan WANG Rongchen ZHAO Yunde | 2019 | Rice science2019，26，2： | 1 |
| 8 | 2D/2D covalent organic framework/CdS Z-scheme heterojunction for enhanced photocatalytic H_(2) evolution:Insights into interfacial charge transfer mechanism显示文摘Covalent organic frameworks(COFs)with high crystallinity and flexible designability have been consid-ered as promising candidates for photocatalytic hydrogen evolution.However,the existence of unpropi-tious exciton effects in COFs leads to poor charge separation,and thus results in low photocatalytic effi-ciency.Herein,to improve the photoelectron migration efficiency,we designed a 2D/2D organic/inorganic direct Z-scheme COF-based heterojunction(TpTAP/CdS),by the in-situ growing of CdS nanosheets on the COF copolymerized via 2,4,6-tris(4-aminophenyl)-1,3,5-triazine(TAP)and 1,3,5-triformylphloroglucinol(Tp).The femtosecond transient absorption(fs-TA)decay kinetics of TpTAP-COF and TpTAP/CdS further reveal the processes of shallow electron trapping and the recombination of the free photogenerated electron-hole pairs.In particular,the transient absorption traces for TpTAP-COF and TpTAP/CdS normal-ized to the photoinduced absorption peak can effectively verify the Z-scheme charge transfer between TpTAP-COF and CdS,which could enhance the charge mobility and separation,thus reducing the pho-tocorrosion of CdS.Additionally,ultraviolet photoelectron spectroscopy(UPS),in-situ X-ray photoelec-tron spectroscopy(XPS),transient photovoltage measurements,and electron spin resonance(ESR)spec-troscopy further confirm the establishment of the internal electric field(IEF).This work demonstrates the important role of COFs in the construction of 2D/2D organic/inorganic direct Z-scheme heterojunctions and offers a new avenue to explain the criticality of dynamics of the photogenerated carriers for the construction of Z-scheme heterojunctions. | Ruiqi Gao Junxian Bai Rongchen Shen Lei Hao Can Huang Lei Wang Guijie Liang Peng Zhang Xin Li | 2023 | Journal of Materials Science & Technology2023，，6： | 1 |
| 9 | Revolutionize Genetic Studies and Crop Improvement with High-Throughput and Genome-Scale CRISPR/Cas9 Gene Editing Technology显示文摘 | Yang, Ning Wang, Rongchen Zhao, Yunde | 2017 | Molecular Plant2017，10，9： | 1 |
| 10 | Alkaline Phosphatase-Initiated Sensitive Responsiveness of Activatable Probes to Hydrogen Sulfide for Accurate Cancer Imaging and Differentiation显示文摘Optical imaging with molecular probes is becoming an essential tool for advancing biological research and clinical applications.However,most currently available molecular probes show limited sensitivity,specificity,and accuracy due to their typical responsiveness to a single stimulation for biomarker-based imaging.In this study,we develop a novel molecular probe that shows alkaline phosphatase(ALP)-instructed sensitive responsiveness to hydrogen sulfide for accurate cancer imaging and differentiation.This designed probe in an aggregated state under physiological conditions bears negatively charged surfaces,giving poor optical response to H_(2)S.The ALP-mediated dephosphorylation reaction yields an assembled product with a positively charged surface,affording significantly aggregation-enhanced responsiveness to H_(2)S with light-up NIR fluorescence at 755 nm.Such charge reversal of assembled probe from negative to positive plays a vital role in allowing precise visualization and differentiation of cancers based on differences in ALP upregulation and H_(2)S content.We envisage that our charge-reversal strategy for multiple-parameter-activated molecule probes will facilitate boosting the specificity and precision of cancer imaging. | Rongchen Wang Kai Yin Muye Ma Tianli Zhu Jinzhu Gao Jie Sun Xuemei Dong Chengjun Dong Xianfeng Gu He Tian Chunchang Zhao | 2022 | CCS Chemistry2022，4，12： | 0 |
| 11 | A dual-modality hydrogen sulfide-specific probe integrating chemiluminescence with NIR fluorescence for targeted cancer imaging显示文摘Taking apart in numerous physiological and pathological activities,hydrogen sulfide(H_(2)S)has been selected as an excellent target spot for the early diagnosis of cancer.So far,there are many mature probes that apply single optical imaging to monitor endogenous H_(2)S.Nevertheless,a single modality is not an ideal method to afford accurate diagnostic information in comprehensive biological organisms.Herein,we developed a dual-modal imaging probe BWS.This designed probe showed a specific response to H_(2)S with both chemiluminescence and NIR fluorescence light-up.The concurrence of fluorescence and chemiluminescence signal provided“double insurances”for highly accurate monitoring of H_(2)S.Satisfactorily,this dual-modal imaging probe performed precise visualization of endogenous H_(2)S in living cells and in vivo.We envisaged that this chemiluminescence/fluorescence real-time dual-modality strategy for H_(2)S detection will expand and optimize the multimodal imaging methods for efficient diagnosis and treatment of cancer. | Xuemei Dong Lixin Sun Ziwen Zhang Tianli Zhu Jie Sun Jinzhu Gao Chengjun Dong Rongchen Wang Xianfeng Gu Chunchang Zhao | 2023 | Science China Chemistry2023，66，6： | 0 |
| 12 | Enhancing bioactivity and stability of polymer-based materialtissue interface through coupling multiscale interfacial interactions with atomic-thin TiO2 nanosheets显示文摘Stable and bioactive material-tissue interface(MTF)basically determines the clinical applications of biomaterials in wound healing,sustained drug release,and tissue engineering.Although many inorganic nanomaterials have been widely explored to enhance the stability and bioactivity of polymer-based biomaterials,most are still restricted by their stability and biocompatibility.Here we demonstrate the enhanced bioactivity and stability of polymer-matrix bio-composite through coupling multiscale material-tissue interfacial interactions with atomically thin TiO_(2)nanosheets.Resin modified with TiO_(2)nanosheets displays improved mechanical properties,hydrophilicity,and stability.Also,we confirm that this resin can effectively stimulate the adhesion,proliferation,and differentiation into osteogenic and odontogenic lineages of human dental pulp stem cells using in vitro cell-resin interface model.TiO_(2)nanosheets can also enhance the interaction between demineralized dentinal collagen and resin.Our results suggest an approach to effectively up-regulate the stability and bioactivity of MTFs by designing biocompatible materials at the sub-nanoscale. | Rongchen Xu Xiaodan Mu Zunhan Hu Chongzhi Jia Zhenyu Yang Zhongliang Yang Yiping Fan Xiaoyu Wang Yuefeng Wu Xiaotong Lu Jihua Chen Guolei Xiang Hongbo Li | 2023 | Nano Research2023，16，4： | 0 |
| 13 | 巨噬细胞促红细胞生成素信号通路促进凋亡细胞清除及免疫耐受显示文摘机体每秒钟约有10^6个细胞凋亡,如被及时清除则维持机体稳定,反之则导致自身免疫病、慢性炎症、发育异常等,但凋亡细胞吞噬清除机制目前尚未阐明。 | Bangwei Luo Woting Gan Zongwei Liu Zigang Shen Jinsong Wang Rongchen Shi Yuqi Liu Yu Liu Man Jiang 张志仁 吴玉章 | 2017 | 科学新闻2017，19，4： | 0 |
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