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| 1 | Polymer-based synthetic oncolytic virus-like nanoparticles for cancer immunotherapy显示文摘Oncolytic viruses have emerged as new powerful therapeutic agents for cancer therapy by specifically lysing cancer cells while activating innate immune responses at the same time.However,due to the thorny issues of safety concerns and host immune reaction,the clinical application of oncolytic viruses is still limited.Herein,we report a rationally designed oncolytic virus-like nanoparticles(OV-NPs)composed of stimulator of interferon genes(STING)-stimulating polymer loaded with therapeutic genes for cancer immunotherapy.After injection into tumor,the OV-NPs carrying OX40L plasmid could reprogram tumor cells to express OX40L immune checkpoint molecules and activate the STING pathway for cooperatively enhancing antitumor immunity,with a tumor suppression rate of 92.3%in B16F10 tumor model and 78.7%in MC38 tumor model without causing any toxicity.The OV-NPs could be further applied in carrying other plasmids(IL-12)and utilization in gene combination therapy.This study should inspire designing synthetic OV-NPs as alternative strategies for extending oncolytic virus application in cancer immunotherapy. | Yuxi Gao Hanqin Zhao Jiayu Zhao Sheng Ma Xinghui Si Liping Liu Ruirui Qiao Wantong Song Xuesi Chen | 2023 | Science China Chemistry2023，66，12： | 0 |
| 2 | 2014-2017年华北平原PM_(2.5)组分消光与能见度对比研究显示文摘华北平原大气污染与低能见度状况一直是人们关切的问题.本文通过分析2014-2017年PM_(2.5)化学成分的浓度和消光效果,研究了华北平原典型城市保定市的大气污染特征.结果表明,PM_(2.5)分的年均浓度显示下降趋势,水溶性无机离子,碳质气溶胶和金属元素分别减少了11μgm^(-3),23μg m^(-3)和1796 ngm^(-3).NH_(4)^(+),NO_(3)^(-)和SO_4^(2-)是PM_(2.5)污染的主要污染物,三者之和占总离子浓度的82.9%.基于IMPROVE方程对细颗粒物进行重构,在观测期间PM_(2.5)质量浓度平均为93±69μgm^(-1),春季,夏季,秋季和冬季的消光系数分别为373.8±233.6 Mm^(-1)±,405.3±300.1 Mm^(-1),554.3±378.2 Mm^(-1)和1005.2±750.3 Mm^(-1).硫酸铵,硝酸铵和有机物对消光的贡献最大,不同季节下占比达55%~77%.通过PM_(2.5)组分进行重构,利用IMPROVE算法计算得到Rb_(sca),用能见度测量值转换得到Vb_(sca),二者具有较高的相关性(r^(2)=0.84);但存在Vb_(sca)的高值被低估,Vb_(sca)的低值被高估的现象;特别是当Rb_(sca)>1123 Mm^(-1)(对应能见度约小于2.0 km)时,Vb_(sca)的值被低估了17.6%.高浓度PM_(2.5)和高湿度对IMPROVE算法结果有显著的影响. | Xinrui Wu Jinyuan Xin Xiaoling Zhang Ruirui Si Guangjing Liu An’na Li Tianxue Wen Zirui Liu Shigong Wang Guangzhou Fan Yuesi Wang Lili Wang Wenkang Gao | 2021 | Atmospheric and Oceanic Science Letters2021，14，2： | 0 |
| 3 | Prokaryotic expression of goldfish Tgf2 transposase with optimal codons and its enzyme activity显示文摘Tgf2 transposase(Tgf2-TPase),a hAT transposase from goldfish,plays an important role in fish transgenic applications.Previously,the production of the recombinant Tgf2-TPase protein required rigorous fermentation at low temperatures(22℃)and early log phase induction(OD600=0.3–0.4)in Rosetta 1(DE3)Escherichia coli lines.In order to better express the Tgf2-TPase and detect its enzyme activity,83 rare codons in Tgf2-TPase were optimized and designated Tgf2-TPase^(83).The expression results showed that the soluble recombinant Tgf2-TPase83 was highly expressed at 30℃ and was inducible at an OD600 of 0.5–0.6 in the same prokaryotic expression system.After purification by affinity chromatography,Tgf2-TPase83 with codon optimization had higher enzyme activity than the Tgf2-TPase control.Comparison of different preservation methods(freezedrying at−80℃,storage in 20%-glycerol,8%-sucrose,4%-mannitol),revealed storage of Tgf2-TPase^(83) in glycerol helped to preserve its DNase digestion activity.Furthermore,size exclusion chromatography suggested that the purified Tgf2-TPase^(83) could recognize and bind to DNA probes containing a terminal inverted repeat(TIR)and a subterminal repeat(STR)sequence of the Tgf2 transposon.Overall,the results showed that optimizing the 83 codons of Tgf2 transposase can simplify the fermentation process and improve the enzyme activity.We propose that the production of the Tgf2-Tpase83 protein in a soluble and active form could provide an alternative tool for genetic modification of fish. | Xi Zhao Ruirui Si Mingjuan He Xiayun Jiang Shuming Zou | 2019 | Aquaculture and Fisheries2019，4，3： | 0 |
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