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| 1 | Purification of total DNA extracted from activated sludge显示文摘从活性污泥样品提取的全部的脱氧核糖核酸的纯化被学习。从活性污泥提取的全部的脱氧核糖核酸的产量和纯净上的抽取缓冲区和细胞溶解处理(溶解酵素,钠 dodecyl 硫酸盐(SDS ) , sonication,机械工厂和热吃惊) 的效果被调查。SDS 和机械工厂是为房间细胞溶解的最有效的方法,这被发现,并且,两个都由 SDS 和热吃惊给了最高的脱氧核糖核酸收益,最纯的脱氧核糖核酸摘录能被获得。有另外的细胞溶解处理的 SDS 的联合例如 sonication 和热吃惊,能显然增加脱氧核糖核酸收益而且导致严重的砍。为粗略的脱氧核糖核酸摘录的纯化,聚乙烯化合物的 polypyrrolidone 被用于腐殖的沾染物的移动。Cetyltrimethyl 铵溴化物,钾醋酸盐和酚 / 氯仿被用来把蛋白质和多糖从粗略的脱氧核糖核酸移开。粗略的脱氧核糖核酸被 isopropanol 降水进一步净化。因此,一个合适的协议为脱氧核糖核酸抽取被建议,产出大约 49.9 mg (全部的脱氧核糖核酸)/g 不稳定的推迟的固体,和脱氧核糖核酸摘录成功地为 16S rDNA 和 16S rDNA V3 区域在 PCR 扩大被使用。16S rDNA V3 区域的 PCR 产品允许 DGGE 分析(使中毒的坡度胶化电气泳动) 可能。 | SHAN Guobin JIN Wenbiao Edward KHLAM XING Xinhui | 2008 | Journal of Environmental Sciences2008，20，1： | 8 |
| 2 | Effects of droplet size and spray volume parameters on droplet deposition of wheat herbicide application by using UAV显示文摘With the characteristic of flexible and precise,unmanned aerial vehicles(UAVs)for low volume applications are increasing substantially and quickly around the globe.However,little attention has been paid to the study of wheat herbicides with UAV,especially the research on the spray volume and droplet size of the herbicide sprayed by UAVs.The objectives of this study were to compare the droplet deposition from a typical commercial UAV under four different spray volumes of 7.5 L/hm2,15.0 L/hm2,22.5 L/hm2,and 30.0 L/hm2 and three different volume median diameter(VMD)of 150μm,200μm,and 300μm during winter wheat weeding period.DepositScan software was used to analyze droplet deposition parameters including the percentage of spray coverage and the number of droplets in various sampling positions.The test results showed that the droplet deposition waseffected by each factor andtheirinteractions.When the spray volume was 7.5 L/hm2,the effect of VMD on the percentage of spray coverage was not significant.However,these variation rules were changed to smaller droplets with greater coverage when the spray volume higher than 15.0 L/hm2.In all treatments,the number of droplets increased with decreasing VMD or increasing spray volume.The maximum percentage of spray coverage and the number of droplets that were achieved under the VMD of 150μm and the spray volume of 30.0 L/hm2 were 12.8%and 40.0 droplets/cm2,respectively.The variation coefficients of the percentage of spray coverage and the number of droplets were 29.0%-73.3%and 20.2%-54.1%,respectively.The most uniform deposition was achieved under the spray volume of 15.0 L/hm2and the VMD of 150μm.The results revealed the effect of droplet size and spray volume parameters on droplet deposition,which was useful for guiding farmers on how to use UAVs for weeding in winter wheat fields. | Changfeng Shan Guobin Wang Haihong Wang Yingjie Xie Huizheng Wang Shilong Wang Shengde Chen Yubin Lan | 2021 | International Journal of Agricultural and Biological Engineering2021，14，1： | 3 |
| 3 | Nanomaterials for environmental burden reduction,waste treatment,and nonpoint source pollution control:a review显示文摘Nanomaterials are applicable in the areas of reduction of environmental burden,reduction/treatment of industrial and agricultural wastes,and nonpoint source(NPS)pollution control.First,environmental burden reduction involves green process and engineering,emissions control,desulfurization/denitrification of nonrenewable energy sources,and improvement of agriculture and food systems.Second,reduction/treatment of industrial and agricultural wastes involves converting wastes into products,groundwater remediation,adsorption,delaying photocatalysis,and nanomembranes.Third,NPS pollution control involves controlling water pollution.Nanomaterials alter physical properties on a nanoscale due to their high specific surface area to volume ratio.They are used as catalysts,adsorbents,membranes,and additives to increase activity and capability due to their high specific surface areas and nano-sized effects.Thus,nanomaterials are more effective at treating environmental wastes because they reduce the amount of material needed. | Guobin SHAN Rao Y.SURAMPALLI Rajeshwar D.TYAGI Tian C.ZHANG | 2009 | Frontiers of Environmental Science & Engineering2009，3，3： | 2 |
| 4 | Separation of Polycyclic Aromatic Compounds from Model Gasoline by Magnetic Alumina Sorbent Based on π-Complexation显示文摘 | Shan Guobin Liu Huizhou Xing Jianmin | | 0，，03： | 1 |
| 5 | Surface modification of 5' - AI2 03 nano - particles with gum arabic and its applications in adsorption and biodesulfurization 显示文摘 | HuaiYing Zhang GuoBin Shan HuiZhou Liu JianMin Xing | 2007 | Surface & Coatings Technology2007，201，： | 1 |
| 6 | Construction,identification and biological feature study of human hepatocellular carcinoma cells stably expressing HBeAg显示文摘Objective: To construct a HepG2 cell line which stably expressing Hepatitis B e antigen(HBeAg)and investigate the effects of HBeAg on the proliferation,migration and invasion of HepG2 cells. Methods: The lentivirus carrying HBeAg gene was constructed and packaged. HepG2 cells were infected with the lentivirus and screened with puromycin to obtain HepG2 cells which stably expressing HBeAg(HepG2-HBeAg cells).The expression levels of HBeAg mRNA and protein were detected by RT-qPCR and Western blot,respectively. The content of HBeAg secretion in cell supernatant in both HepG2-HBeAg cells and control cells(HepG2-NC cells and HepG2 cells)were detected by IFMA assay. Furthermore,CCK-8 proliferation assay,colony formation assay and transwell migration and invasion assays were conducted to compare the abilities of cell proliferation,migration and invasion,respectively. Results: The expression of HBeAg in the HepG2-HBeAg cell was significantly higher than those in HepG2 cells and HepG2-NC cells. Secreted HBeAg in the supernatant of HepG2-HBeAg cells was 26. 33±2. 13 PEIU/mL but was undetectable in supernatant of control cells. The proliferation,migration and invasion were all significantly lower in HepG2-HBeAg cells compared to control cells(P<0. 01). Conclusion: A HepG2 cell line which stably expressing HBeAg was constructed successfully,and the over-expression of HBeAg could attenuate the proliferation,migration and invasion of hepatocellular carcinoma cells. | Yuxuan Li Shan Huang Kezhi Li Guobin Wu Hao Tian Yinnong Zhao | 2017 | 广西医科大学学报2017，34，12： | 0 |
| 7 | M6PR interacts with the HA2 subunit of influenza A virus to facilitate the fusion of viral and endosomal membranes显示文摘Influenza A virus(IAV) commandeers numerous host cellular factors for successful replication. However, very few host factors have been revealed to be involved in the fusion of viral envelope and late endosomal membranes. In this study, we identified cation-dependent mannose-6-phosphate receptor(M6PR) as a crucial host factor for the replication of IAV. We found that siRNA knockdown of M6PR expression significantly reduced the growth titers of different subtypes of IAV, and that the inhibitory effect of M6PR siRNA treatment on IAV growth was overcome by the complement of exogenously expressed M6PR. When A549 cells were treated with siRNA targeting M6PR,the nuclear accumulation of viral nucleoprotein(NP) was dramatically inhibited at early timepoints post-infection, indicating that M6PR engages in the early stage of the IAV replication cycle. By investigating the role of M6PR in the individual entry and post-entry steps of IAV replication, we found that the downregulation of M6PR expression had no effect on attachment, internalization, early endosome trafficking,or late endosome acidification. However, we found that M6PR expression was critical for the fusion of viral envelope and late endosomal membranes. Of note, M6PR interacted with the hemagglutinin(HA) protein of IAV, and further studies showed that the lumenal domain of M6PR and the ectodomain of HA2 mediated the interaction and directly promoted the fusion of the viral and late endosomal membranes,thereby facilitating IAV replication. Together, our findings highlight the importance of the M6PR–HA interaction in the fusion of viral and late endosomal membranes during IAV replication. | Yuzhen Hu Li Jiang Guangwen Wang Yangming Song Zhibo Shan Xuyuan Wang Guohua Deng Jianzhong Shi Guobin Tian Xianying Zeng Liling Liu Hualan Chen Chengjun Li | 2024 | Science China(Life Sciences)2024，67，3： | 0 |
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