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| 1 | Molecular phylogeny and species delimitation of Stachyuraceae: Advocating a herbarium specimen-based phylogenomic approach in resolving species boundaries显示文摘Species concept and delimitation are fundamental to taxonomic and evolutionary studies.Both inadequate informative sites in the molecular data and limited taxon sampling have often led to poor phylogenetic resolution and incorrect species delineation.Recently,the whole chloroplast genome sequences from extensive herbarium specimen samples have been shown to be ffective to amend the problem.Stachyuraceae are a small family consisting of only one genus Stachyurus of six to 16 species.However,species delimitation in Stachyurus has been highly controversial because of few and generally unstable morphological characters used for cassfcation.In this study,we sampled 69 individuals of seven species(each with at least three individual)covering the entire taxonomlc dlversity,geographlc range,and morphological varlation of Stachyurus from her barium specimens for genome wide plastid gene sequencing to address species delineation in the genus.We obtained high quality DNAS from specimens using a repently developed DNA reconstruction technique.We first assembled four whole chloroplast genome sequences.Based on the chloroplast genome and one nuclear ribosomal DNA sequence of Stachyunus,we designed primers for multiplex polymerase chain reaction and high throughput sequencing of 44 plastid loci for species of Stachyurus,Data of these chloroplast DNA and nuclear ribosomal DNA internal transcribed spacer sequences were used for phylogenetic analyses.The phylogenetic results showed that the Japanese species Stachyurus praecox Siebold&Zucc.was sister to the rest in China's Mainland,which indicated a typical Sino-Japanese distribution pattern.Based on diagnostic morphological characters,dlstinct distrbutional range,and monophyly of each clade,we redefined seven species for Stachyurus fllowving an integrative specles concept,and revised the taxonomy of the family based on previous reports and specimens,in particular the type specimens.Furthermore,our divergence time estimation results suggested that Stachyuraceae split from its sister group Crossosomataceae from the New World at ca.54.29 Mya,but extant species of Stachyuraceae started their diversifcation only recently at ca.6.85 Mya.Diversification time of Stachyurus in China's Mainland was estimated to be ca.4.45 Mya.This research has provided an example of using the herbarium specimen based phylogenomic approach in resolving species boundaries in a taxonomically dificult genus. | Jun-Xia Su Cong-Cong Dong Yan-Ting Niu Li-Min Lu Chao xu Bing Liu ShiLiang Zhou An-Ming Lu Yu-Ping Zhu Jun Wen Zhi-Duan Chen | 2020 | Journal of Systematics and Evolution2020，58，5： | 2 |
| 2 | Assessing land ecological security in Shanghai(China)based on catastrophe theory显示文摘 | Su Shiliang Li Dan Yu Xiang | 2011 | Stoch Environ Res Risk Assess2011，25，： | 1 |
| 3 | Integrative fuzzy set pair model for land ecological security assessment:a case study of Xiaolangdi Reservoir Region,China显示文摘 | Su Shiliang Chen Xia Stephen D DeGloria | 2010 | Stoch Environ Res Risk Assess2010，24，： | 1 |
| 4 | Assessing land ecological security in Shanghai (China) based on catastrophe theory显示文摘 | SU Shiliang LI Dan YU Xiang | 2011 | Stochastic Environmental Research and Risk Assessment2011，25，6： | 1 |
| 5 | Rural settlement exp- ansion and paddy soil loss across an ex-urbanizing watershed in eastern coastal China during market transition显示文摘 | SU Shiliang ZHANG Qi ZHANG Zhonghac | | Reg Environ Change0，201，11： | 1 |
| 6 | Assessing land ecological security in Shanghai(China) based on catastrophe theory显示文摘 | Su Shiliang Li Dan Yu Xiang | 2011 | Stochastic Environmental Research and Risk Assessment2011，25，6： | 1 |
| 7 | Assessing land ecological security in Shanghai (China) based on catastrophe theory显示文摘 | SU Shiliang LI Dan YU Xiang | 2012 | Stochastic Environmental Research and Risk Assessment2012，25，： | 1 |
| 8 | Spatio-temporal patterns and source apportionment of pollution in Qiantang River (China) using neural-based modeling and multivariate statistical techniques显示文摘 | Shiliang Su Junjun Zhi Liping Lou Fang Huang Xia Chen Jiaping Wu | 2010 | Physics and Chemistry of the Earth2010，，9： | 1 |
| 9 | A new average response spectrum method for linear response analysis of structures to spatial earthquake ground motions显示文摘 | Su Liang Dong Shiliang Kato S | 2006 | Engineering Structure2006，28，13： | 1 |
| 10 | Multi-scale analysis of spatially varying relationships between agricultural landscape pat- terns and urbanization using geographically weighted regression 显示文摘 | SU Shiliang XIAO Rui ZHANG Yuan | 2012 | Applied geography2012，，2： | 1 |
| 11 | Temporal trend and source apportionment of water pollution in different functional zones of Qiantang River, China显示文摘 | Shiliang Su Dan Li Qi Zhang Rui Xiao Fang Huang Jiaping Wu | 2010 | Water Research2010，，4： | 1 |
| 12 | Assessing land ecologi- cal security in shanghai (china) based on catastrophe theory 显示文摘 | Shiliang Su Dan Li Xiang Yu | 2011 | Springer2011，，25： | 1 |
| 13 | Retrieving canopy height and density of paddy rice from Radarsat-2 images with a canopy scattering model显示文摘 | Yuan Zhang Xiaohui Liu Shiliang Su Cuizhen Wang | 2014 | International Journal of Applied Earth Observations and Geoinformation2014，，： | 1 |
| 14 | Integrative fuzzy set pair model for land ecological security assessment:a case study of Xiaolangdi Reservoir Region,China显示文摘 | Shiliang Su Xia Chen Stephen D | | 0，，05： | 1 |
| 15 | Design, Synthesis and SAR Studies of Novel and Potent Dipeptidyl Peptidase 4 Inhibitors显示文摘Dipeptidyl peptidase 4(DPP-4)is a clinically validated target for the treatment of type 2 diabetes mellitus(T2DM).To discover novel and potent DPP-4 inhibitors,three series of compounds were designed and synthesized in this study based on our previously identified novel scaffold of 2-phenyl-3,4-dihydro-2H-benzo[f]chromen-3-amine.Among the designed compounds,41d-1 was the most po tent one with an IC_(50) value of 16.00 nM. | Na Luo Xiaoyu Fang Mingbo Su Xinwen Zhang Dan Li Honglin Li Shiliang Li Zhenjiang Zhao | 2021 | Chinese Journal of Chemistry2021，39，1： | 0 |
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