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| 1 | Clinical efficacy and safety of moxifloxacin versus levofloxacin plus metronidazole for community-acquired pneumonia with aspiration factors显示文摘 | Sun Tieying Sun Li Wang Rongmei Ren Xiaoping Sui Dong-jiang Pu Chun Ren Yajuan Liu Ying Yang Zhuo Li Fengzhi | 2014 | Chinese Medical Journal2014，，7： | 34 |
| 2 | Chemistry and pharmacology of Siraitia grosvenorii: A review显示文摘Siraitia grosvenorii is a perennial herb endemic to Guangxi province of China. Its fruit, commonly known as Luo hanguo, and has been used for hundreds of years as a natural sweetener and as a traditional medicine for the treatment of pharyngitis, pharyngeal pain, as well as an anti-tussive remedy in China. Based on ninety-three literary sources, this review summarized the advances in chemistry, biological effects, and toxicity research of S. grosvenorii during the past 30 years. Several different classes of compounds have been isolated or detected from various parts of S. grosvenorii, mainly triterpenoids, flavonoids, polysaccharides, amino acids, and essential oils. Various types of extracts or individual compounds derived from this species exhibited a wide array of biological effects e.g. anti-tussive, phlegm-relieving, anti-oxidant, immunomodulatory, liver-protecting, glucose-lowering, and anti-microbial. The existing research has shown that extracts and individual compounds from S. grosvenorii are basically non-toxic. Finally, some suggestions for further research on specific chemical and pharmacological properties of S. grosvenorii are proposed in this review. | LI Chun LIN Li-Mei SUI Feng WANG Zhi-Min HUO Hai-Ru DAI Li JIANG Ting-Liang | 2014 | Chinese Journal of Natural Medicines2014，12，2： | 28 |
| 3 | Molecular Cloning and Expression of Squalene Epoxidase from a Medicinal Plant, Bupleurum chinense显示文摘Objective In plant, squalene epoxidase(SE) catalyzes the first oxygenation step in the biosynthetic pathway of triterpenoid and phytosterol, representing one of the rate-limiting enzymes in this pathway. Bupleurum chinense is an important medicinal herb with its major active constituents such as triterpenoid saponins and saikosaponins. In order to obtain the series of enzymatic genes involved in saikosaponin biosynthesis, a c DNA of SE, designated Bc SE1, was cloned from B. chinense. Methods The Bc SE1 gene was cloned by homology-based PCR and 5'/3' RACE methods from the adventitious roots of B. chinense. The physical and chemical parameters of Bc SE1 protein were predicted by protparam. In order to discover hints in amino acid sequences on the dominant functions in the biosynthesis of saponin or phytosterol, sequences of SE from other plants were downloaded from NCBI for sequences alignment and phylogenetic analysis. Bc SE1 was cloned into a yeast mutant KLN1(MATa, erg1::URA3, leu2, ura3, and trp1) to verify the enzyme activity of Bc SE1. Additionally, the tissue-specific expression and methyl jasmonate(Me JA) inducibility of Bc SE1 were investigated using quantitative real-time PCR. Results The predicted protein of Bc SE1 is highly similar to SEs from other plants sharing amino acid sequence identities of up to 88%. The Bc SE1 can functionally complement with yeast SE gene(ERG1) when expressed in the KLN1 mutant(MATa, erg1::URA3, leu2, ura3, and trp1). Using as controls with β-amyrin synthase(β-AS) which is presumed to catalyze the first committed step in saikosaponin biosynthesis and a cycloartenol synthase(CAS) relating to the phytosterol biosynthesis, the transcript of Bc SE1 was significantly elevated by Me JA in adventitious roots of B. chinense and the transcript of Bc SE1 was most abundant in the fruits and flowers of plants, followed by that in the leaves and roots, and least in stems. Conclusion It is the first time to illustrate the molecular information of SE in B. chinense and to clone the full-length SE gene in plants of genus Bupleurum L. | Ke Gao Jie-sen Xu Jing Sun Yan-hong Xu Jian-he Wei Chun Sui | 2016 | Chinese Herbal Medicines2016，8，1： | 10 |
| 4 | Origin of Baotoudong syenites in North China Craton:Petrological, mineralogical and geochemical evidence显示文摘Baotoudong 正长岩 pluton 被定位到 Baotou 城市的东方,内部蒙古,沿着北方中国 Craton (NCC ) 的北边缘的三叠纪的碱的 magmatic 带的最西的部分。锆石 U-Pb 年龄, petrological,矿物学并且 pluton 的 geochemical 数据在这份报纸被获得,抑制它的起源和披风来源特征。pluton 由 nepheline-clinopyroxene 正长岩和碱长石正长岩组成,与 214.7 ? 的锆石 U-Pb 年龄 ?????????? 潡潴摵湯 ????? 甠瑬慲潰慴獳捩 ???????????????????? | NIU Xiao Lu YANG Jing Sui LIU Fei ZHANG Hong Yu YANG Ming Chun | 2016 | Science China Earth Sciences2016，59，1： | 10 |
| 5 | Application of CRISPR/Cas9 in plant biology显示文摘The CRISPR/Cas(clustered regularly interspaced short palindromic repeats/CRISPRassociated proteins) system was first identified in bacteria and archaea and can degrade exogenous substrates. It was developed as a gene editing technology in 2013. Over the subsequent years, it has received extensive attention owing to its easy manipulation, high efficiency, and wide application in gene mutation and transcriptional regulation in mammals and plants. The process of CRISPR/Cas is optimized constantly and its application has also expanded dramatically. Therefore, CRISPR/Cas is considered a revolutionary technology in plant biology. Here, we introduce the mechanism of the type II CRISPR/Cas called CRISPR/Cas9, update its recent advances in various applications in plants, and discuss its future prospects to provide an argument for its use in the study of medicinal plants. | Xuan Liu Surui Wu Jiao Xu Chun Sui Jianhe Wei | 2017 | Acta Pharmaceutica Sinica B2017，7，3： | 8 |
| 6 | Cloning and Expression Analysis on 3-Hydroxy-3-methylglutaryl-coenzyme A Reductase from Aquilaria sinensis显示文摘Objective To clone the full-length cDNA of 3-hydroxy-3-methylglutaryl-coenzyme A reductase(HMGR)from Aquilaria sinensis(AsHMGR1)and to analyze its expression profile in different tissues and in response to different treatments.HMGR is the first rate-limiting enzyme for sesquiterpene synthesis in the mevalonate pathway.Methods RT-PCR and RACE were used to clone the full-length cDNA of HMGR from A.sinensis based on the conserved HMGR gene fragments.The bioinformatic analysis was performed on its nucleic acid and protein sequence.The expression profile of AsHMGR1 in different tissues and in response to different treatments was analyzed by quantitative RT-PCR.Results The full-length AsHMGR1 cDNA was 2026 bp,containing a 1719 bp open reading frame which encoded a protein of 572 amino acids.Amino acid sequence homology alignment and phylogenetic analysis demonstrated that AsHMGR1 belonged to the HMGR gene family.The detection of tissue expression patterns showed that AsHMGR1 was mainly expressed in the stem,followed by roots and branches.AsHMGR1 could be stimulated by methyl jasmonate and H2O2to varying degrees in a time-dependent manner.Conclusion These data will provide a foundation for further investigation on AsHMGR1 functions and regulatory mechanisms in sesquiterpene synthesis in A.sinensis. | XU Yan-hong YANG Xin WEI Jian-he ZHANG Xing-li ZHANG Zheng GAO Zhi-hui SUI Chun | 2013 | Chinese Herbal Medicines2013，5，3： | 6 |
| 7 | Cell cycle-related kinase reprograms the liver immune microenvironment to promote cancer metastasis显示文摘The liver is an immunologically tolerant organ and a common metastatic site of multiple cancer types.Although a role for cancer cell invasion programs has been well characterized,whether and how liver-intrinsic factors drive metastatic spread is incompletely understood.Here,we show that aberrantly activated hepatocyte-intrinsic cell cycle-related kinase(CCRK)signaling in chronic liver diseases is critical for cancer metastasis by reprogramming an immunosuppressive microenvironment.Using an inducible liverspecific transgenic model,we found that CCRK overexpression dramatically increased both B16F10 melanoma and MC38 colorectal cancer(CRC)metastasis to the liver,which was highly infiltrated by polymorphonuclear-myeloid-derived suppressor cells(PMNMDSCs)and lacking natural killer T(NKT)cells.Depletion of PMN-MDSCs in CCRK transgenic mice restored NKT cell levels and their interferon gamma production and reduced liver metastasis to 2.7% and 0.7%(metastatic tumor weights)in the melanoma and CRC models,respectively.Mechanistically,CCRK activated nuclear factor-kappa B(NF-κB)signaling to increase the PMN-MDSC trafficking chemokine C-X-C motif ligand 1(CXCL1),which was positively correlated with liver-infiltrating PMN-MDSC levels in CCRK transgenic mice.Accordingly,CRC liver metastasis patients exhibited hyperaaivation of hepatic CCRK/NF-κB/CXCL1 signaling,which was associated with accumulation of PMN-MDSCs and paucity of NKT cells compared to healthy liver transplantation donors.In summary,this study demonstrates that immunosuppressive reprogramming by hepatic CCRK signaling undermines antimetastatic immunosurveillance.Our findings offer new mechanistic insights and therapeutic targets for liver metastasis intervention. | Xuezhen Zeng Jingying Zhou Zhewen Xiong Hanyong Sun Weiqin Yang Myth T.S.Mok Jing Wang Jingqing Li Man Liu Wenshu Tang Yu Feng Hector Kwong-Sang W ang Shun-Wa Tsang King-Lau Chow Philip Chun Yeung John Wong Paul Bo-San Lai Anthony Wing-Hung Chan Ka Fai To Stephen Lam Chan Qiang Xia Jing Xue Xiao Chen Jun Yu Sui Peng Joseph Jao-Yiu Sung Ming Kuang Alfred Sze-Lok Cheng | 2021 | Cellular & Molecular Immunology2021，18，4： | 5 |
| 8 | Identification of WRKY Transcription Factors Related to Saikosaponin Biosynthesis in Adventitious Roots of Bupleurum chinense显示文摘Objective To identify the genes of WRKY transcription factors(TFs) from roots of Bupleurum chinense and genes that potentially regulate saikosaponin(SS) biosynthesis.Methods Firstly,the subfamily cluster analysis was mainly based on Arabidopsis thaliana WRKYs for 27 putative WRKY TFs selected from previous transcriptome sequencing data.Secondly,qPCR was used to screen such genes of WRKY TFs that could be induced by NaCI and PEG6000 in adventitious roots of B.chinense.Meanwhile,saikosaponins(SSs) in treated adventitious roots were determined by HPLC.The roots were collected at 0,2,4,8,12,24,48,and 72 h after treatments,and 120 h only for PEG.Finally,the tissue-specific expression was analyzed on screened genes by qPCR.Results The 27 genes were grouped into three categories:There were nine in Group Ⅰ,15 in Group Ⅱ,and two in Group Ⅲ.Four genes of WRKYTFs,BCWRKY6,BCWRKY16,BCWRKY32,and BCWRKY35 were obviously induced by NaCI in adventitious roots of B.chinense,while only BCWRKY32 was induced by PEG.The content of SSs increased at different levels in NaCI and PEG6000 treatment.Three genes including BCWRKY6,BCWRKY32,and BCWRKY35,expressed most in roots,were similar to the accumulation pattern of SS.Conclusion The three WRKY genes,BCWRKY6,BCWRKY32,and BCWRKY35,may be involved in the biosynthesis of SS. | Su-rui Wu Ke Gao Xuan Liu Jiao Xu Jian-he Wei Chun Sui | 2017 | Chinese Herbal Medicines2017，9，2： | 4 |
| 9 | Differences in atheroma between Caucasian and Asian subjects with anterior stroke:A vessel wall MRI study显示文摘Background and purpose While extracranial carotid artery stenosis is more common among Caucasians and intracranial artery stenosis is more common among Asians,the differences in atherosclerotic plaque characteristics have not yet been extensively examined.We sought to investigate plaque location and characteristics within extracranial carotid and intracranial arteries in symptomatic Caucasians and Chinese using vessel wall MRI.Methods Subjects with recent anterior circulation ischaemic stroke were recruited and imaged at two sites in the USA and China using similar protocols.Both extracranial carotid and intracranial arteries were reviewed to determine plaque location and characteristics.Results The prevalence of extracranial carotid plaque in Caucasians and Chinese was 73.1%and 49.1%,respectively(p=0.055).Prevalence of intracranial plaque was 38.5%and 69.1%in Caucasians and Chinese,respectively(p=0.02).Furthermore,42% of Caucasians and 16%of Chinese had high-risk plaque(HRP)features(intraplaque haemorrhage,luminal surface disruption)in the extracranial carotid artery(p=0.03).The prevalence of HRP features in intracranial arteries was not significantly different between the two cohorts(4%vs 11%;p=0.42).Conclusions Differences in the location and characteristics of cerebrovascular atherosclerosis were identified by vessel wall MRI in US Caucasian and Chinese subjects with recent anterior circulation ischaemic stroke.Extracranial carotid plaques with HRP features were more common in Caucasians.Intracranial plaques were more common in Chinese subjects,but no significant difference between the two cohorts in intracranial HRP prevalence was found.Larger studies using vessel wall imaging to investigate racial differences in cerebrovascular disease may inform underlying mechanisms of HRP development and may ultimately help guide appropriate therapy. | Hiroko Watase Mi Shen Binbin Sui Peiyi Gao Dong Zhang Jie Sun Niranjan Balu Daniel S Hippe Gail P Jarvik Xihai Zhao Rui Li Shuo Chen Chun Yuan Thomas S Hatsukami | 2021 | Stroke & Vascular Neurology2021，6，1： | 4 |
| 10 | Characteristics of Long-term Nonprogressors and Viremia Controllers Infected with HIV-1 via Contaminated Blood Donations or Transfusions Conducted 20 Years Earlier显示文摘To characterize long‐term nonprogressors(LTNPs)and viremia controllers(VCs),infected with HIV‐1 through contaminated blood donation or transfusion between 1992 and 1996 in Henan,China.LTNPs and VCs were defined by CD4+T lymphocyte(CD4)count and viral load(VL).Of29,294 patients infected with HIV‐1 via contaminated blood donation or transfusion that had conducted for more than 20 years,92 were LTNPs/VCs.There were 70 LTNPs(0.24%),43 | LIU Jia FAN Pan Ying XUE Xiu Juan YAN Jiang Zhou SUN Guo Qing LIU Chun Hua TIAN Sui An LI Ning SUN Ding Yong ZHU Qian WANG Zhe | 2017 | Biomedical and Environmental Sciences2017，30，12： | 3 |
| 11 | Factors Affecting Embryogenic Callus Production and Plant Regeneration in Anther Culture of Bupleurum chinense显示文摘Objective To evaluate the influences of the genotypes,anther developmental stages,and cultural conditions on the efficiency of embryogenic callus induction and plant regeneration in the anthers culture of Bupleurum chinense.Methods The different effects such as four genotypes,plant growth regulators,and temperature condition were compared in the experiments.The histological study was performed with the process of the anther culture.Results The highest inducing rate of embryogenic calli were achieved for the genotypes Zhongcaiyihao(ZCYH),Z4,and Z5 at the early-to middle-uninucleate stages,except for genotype ZPM1 at the tetrad stage.Cold pretreatment increased the production of the embryogenic callus,in which 4-day cold pretreatment improved the production of embryogenic callus from 0% to 2.2% and 5.0% for genotypes ZPM1 and ZCYH,respectively.No embryogenic callus was induced in the medium containing less than 0.75 mg/L 2,4-dichlorophenoxyacetic acid(2,4-D) .The highest regeneration rate(34.6%) was obtained in 1/2 MS salts regeneration medium supplemented with 0.1 mg/L 6-benzylmaminopurine(BA) .The low concentration of BA was able to promote the embryogenic callus formation and subsequent plantlet regeneration via somatic embryogenesis.Chromosome counting of regenerated plantlets showed mostly diploid plant(2n = 12) with only one haploid plant(n = 6) .Because of the low rate of microspore embryo formation,we only tracked the process of embryogenesis from the connective tissue,instead of microspore by histological observations.Conclusion This study establishes an efficient system for embryogenic callus induction and plant regeneration system.This is the first report on the haploid plantlet through the anther culture of B.chinense. | YANG Cheng-min,ZHAO Yu-kai,WEI Jian-he,ZHAO Li-zi,SUI Chun,ZHANG Zheng,CUI Lu-ying Institute of Medicinal Plant Development,Chinese Academy of Medical Sciences & Peking Union Medical College,Beijing 100193,China | 2011 | Chinese Herbal Medicines2011，3，3： | 3 |
| 12 | First Report of Corynespora Leaf Spot on Patchouli Caused by Corynespora cassiicola in China显示文摘 | CHEN Xu-yu SUI Chun GAN Bing-chun | 2010 | Plant Dis2010，84，12： | 1 |
| 13 | The Mechanism and Characterization of Dye-Free Coloration on Tussah Silk Based on Tryptophan显示文摘 | Sui Yan Ling Wei Chun Yan Liu Wen Jing Wang Bo Li Shao Quan Cui Yong Zhu | 2011 | Advanced Materials Research2011，，175： | 1 |
| 14 | Modest increase in risk of specific types of cancer types in type 2 diabetes mellitus patients显示文摘 | Sui‐Foon Lo Shih‐Ni Chang Chih‐Hsin Muo Shih‐Yin Chen Fang‐Yin Liao Shu‐Wei Dee Pei‐Chun Chen Fung‐Chang Sung | 2012 | Int J Cancer2012，，1： | 1 |
| 15 | Development of a Stable Male-sterile Line and Its Utilization in High Yield Hybrid of Platycodon Grandiflorum显示文摘 | Feng-Hua Shi Chun Sui Cheng-Min Yang | 2011 | Journal of Medicinal Plants Research2011，，： | 1 |
| 16 | Transcriptome analysis of Bupleurumchinense focusing on genes involved in the biosynthesis of saikosaponins 显示文摘 | Sui Chun Jie Zhang Jianhe Wei | 2011 | BMC Genomics2011，12，： | 1 |
| 17 | Development of genomic SSR and potential EST-SSR markers in Bupleurum chinense DC显示文摘 | Chun Sui Wei Jianhe Chen Shilin | 2009 | Afr J Biotechnol2009，8，22： | 1 |
| 18 | First report of broad bean wilt virus 2 infecting Bupleurum chinense in China 显示文摘 | Chun Sui Wei Jianhe Zhan Qingqing | 2009 | Plant Dis2009，93，8： | 1 |
| 19 | Construction of a full-length enriched cDNA library and analysis of 3111 ESTs from roots of Bupleurum chinense DC显示文摘 | Chun Sui Wei Jianhe Chen Shilin | 2010 | Bot Stud2010，51，1： | 1 |
| 20 | Antiproliferative and apoptotic effects of Peonol on human hepatocellular carcinoma cells显示文摘 | Chun H Z Sui Y H Mei Q C | 2008 | Anticancer Drugs2008，19，4： | 1 |
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