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| 1 | Distant hybridization leads to different ploidy fishes显示文摘Distant hybridization makes it possible to transfer the genome of one species to another, which results in changes in phenotypes and genotypes of the progenies. This study shows that distant hybridization or the combination of this method with gynogenesis or androgenesis lead to different ploidy fishes with genetic variation, including fertile tetraploid hybrids, sterile triploid hybrids, fertile diploid hybrids, fertile diploid gynogenetic fish, and their derived progenies. The formations of the different ploidy fishes depend on the genetic relationship between the parents. In this study, several types of distant hybridization, including red crucian carp (Carassius auratus red var.) (2n=100, abbreviated as RCC) (♀)×common carp (Cyprinus carpio L.) (2n=100, abbreviated as CC) (♂), and RCC (2n=100) (♀)×blunt snout bream (Megalobrama amblycephala) (2n=48, abbreviated as BSB) (♂) are described. In the distant hybridization of RCC (♀)×CC (♂), bisexual fertile F3–F18 allotetraploid hybrids (4n=200, abbreviated as 4nAT) were formed. The diploid hybrid eggs and diploid sperm generated by the females and males of 4nAT developed into diploid gynogenetic hybrids and diploid androgenetic hybrids, respectively, by gynogenesis and androgenesis, without treatment for doubling the chromosome. Improved tetraploid hybrids and improved diploid fishes with genetic variation were derived from the gynogenetic hybrid line. The improved diploid fishes included the high-body RCC and high-body goldfish. The formation of the tetraploid hybrids was related to the occurrence of unreduced gametes generated from the diploid hybrids, which involved in premeiotic endoreduplication, endomitosis, or fusion of germ cells. The sterile triploid hybrids (3n=150) were produced on a large scale by crossing the males of tetraploid hybrids with females of diploid fish (2n=100). In another distant hybridization of RCC (♀)×BSB (♂), different ploidy fishes were obtained, including diploid bisexual fertile natural gynogenetic fish (2n=100), sterile triploid hybrids (3n=124), and bisexual fertile tetraploid hybrids (4n=148). Furthermore, two kinds of pentaploid hybrids (5n=172 and 5n=198) were formed. The biological characteristics and the mechanisms of formation of the different ploidy fish were compared and discussed at the cellular and molecular level. The results indicated distant hybridization or the combination of this method with gynogenesis or androgenesis affects the formation of different ploidy fish with genetic variation. | LIU ShaoJun Key Laboratory of Protein Chemistry and Fish Developmental Biology of Education Ministry of China, College of Life Sciences, Hunan Normal University, Changsha 410081, China | 2010 | Science China(Life Sciences)2010，53，4： | 39 |
| 2 | Development and application of biological technologies in fish genetic breeding显示文摘Fish genetic breeding is a process that remolds heritable traits to obtain neotype and improved varieties.For the purpose of genetic improvement,researchers can select for desirable genetic traits,integrate a suite of traits from different donors,or alter the innate genetic traits of a species.These improved varieties have,in many cases,facilitated the development of the aquaculture industry by lowering costs and increasing both quality and yield.In this review,we present the pertinent literatures and summarize the biological bases and application of selection breeding technologies(containing traditional selective breeding,molecular marker-assisted breeding,genome-wide selective breeding and breeding by controlling single-sex groups),integration breeding technologies(containing cross breeding,nuclear transplantation,germline stem cells and germ cells transplantation,artificial gynogenesis,artificial androgenesis and polyploid breeding)and modification breeding technologies(represented by transgenic breeding)in fish genetic breeding.Additionally,we discuss the progress our laboratory has made in the field of chromosomal ploidy breeding of fish,including distant hybridization,gynogenesis,and androgenesis.Finally,we systematically summarize the research status and known problems associated with each technology. | XU Kang DUAN Wei XIAO Jun TAO Min ZHANG Chun LIU Yun LIU ShaoJun | 2015 | Science China(Life Sciences)2015，58，2： | 23 |
| 3 | Establishment of the diploid gynogenetic hybrid clonal line of red crucian carp × common carp显示文摘This study investigated the gynogenetic cytobiological behavior of the third gynogenetic generation (G3), which was generated from the diploid eggs produced by the second gynogenetic generation (G2) of red crucian carp × common carp, and determined the chromosomal numbers of G3, G2×scatter scale carp and G2×allotetraploid hybrids of red crucian carp × common carp. The results showed that the diploid eggs of G2 with 100 chromosomes, activated by UV-irradiated sperm from scatter scale carp and without the treatment for doubling the chromosomes, could develop into G3 with 100 chromosomes. Similar to the first and second gynogenetic generations (G1 and G2), G3 was also diploid (2n=100) and presented the hybrid traits. The triploids (3n=150) and tetraploids (4n=200) were produced by crossing G2 with scatter scale carp and crossing G2 with allotetraploids, respectively. The extrusion of the second polar body in the eggs of G2 ruled out the possibility that the retention of the second polar body led to the formation of the diploid eggs. In addition, we discussed the mechanism of the formation of the diploid eggs generated by G2. The establishment of the diploid gynogenesis clonal line (G1, G2 and G3) provided the evidence that the diploid eggs were able to develop into a new diploid hybrid clonal line by gynogenesis. By producing the diploid eggs as a unique reproductive way, the diploid gyno- genetic progeny of allotetraploid hybrids of red crucian carp × common carp had important signifi- cances in both biological evolution and production application. | LIU ShaoJun, DUAN Wei, TAO Min, ZHANG Chun, SUN YuanDong, SHEN JiaMin, WANG Jing, LUO KaiKun & LIU Yun Key Laboratory of Protein Chemistry and Fish Developmental Biology of Education Ministry of China, College of Life Sciences, Hunan Normal University, Changsha 410081, China | 2007 | Science China(Life Sciences)2007，50，2： | 21 |
| 4 | Development of Mining Technology and Equipment for Seafloor Massive Sulfide Deposits显示文摘Seafloor massive sulfide(SMS) deposits which consist of Au, Ag, Cu, and other metal elements, have been a target of commercial mining in recent decades. The demand for established and reliable commercial mining system for SMS deposits is increasing within the marine mining industry. The current status and progress of mining technology and equipment for SMS deposits are introduced. First, the mining technology and other recent developments of SMS deposits are comprehensively explained and analyzed.The seafloor production tools manufactured by Nautilus Minerals and similar mining tools from Japan for SMS deposits are compared and discussed in turn. Second, SMS deposit mining technology research being conducted in China is described, and a new SMS deposits mining tool is designed according to the environmental requirement. Finally, some new trends of mining technology of SMS deposits are summarized and analyzed. All of these conclusions and results have reference value and guiding significance for the research of SMS deposit mining in China. | LIU Shaojun HU Jianhua ZHANG Ruiqiang DAI Yu YANG Hengling | 2016 | Chinese Journal of Mechanical Engineering2016，29，5： | 19 |
| 5 | MiR-135a promotes growth and invasion of colorectal cancer via metastasis suppressor 1 in vitro显示文摘MicroRNAs (miRNAs ) 是参予送信人 RNA 和蛋白质合成的空间与时间的规定的小非编码的 RNA。异常 miRNA 表示导致发展畸形和疾病。miR-135a 被认为是 oncogenic;然而,在 colorectal 癌症(CRC ) 的 miR-135a 的功能和机制大部分是未知的。因此,我们调查了 miR-135a 的功能和机制,特别与转移 suppressor 的它的关系 1 (MTSS1 ) 在 CRC 的基因。当它房间增长,移植,和侵略上的效果被 MTT 决定时, miR-135a 的表达式被即时聚合酶链反应分别地决定,没有并且与 matrigel。MTSS1 的表示被西方的污点分析检测。miR-135a 表示比在非肿瘤控制织物在人的 CRC 样品是更高的,这被发现。用 SW480 和 SW620 CRC 房间行,增加了增长响应 miR-135a 被观察。我们也证明分别地, miR-135a CRC 房间有或没有 Matrigel 经由 transwell 试金支持了活动性和侵略。相反, miR-135a 的抑制减少了他们的 proliferative 和侵略能力。MTSS1 被酶报告试金作为 miR-135a 的候选人目标基因识别。西方的污点分析证明 MTSS1 的表示被 miR-135a overexpression 调整并且击倒。同样,在 MTSS1 被小介入 RNA 击倒以后, miR-135a-mediated 房间活动性和侵略被减少。这些数据显示 miR-135a 支持 CRC 细胞的生长和侵略,至少部分,通过指向 MTSS1。 | Weiwei Zhou Xiayu Li Fen Liu Zhiming Xiao Miao He Shourong Shen Shaojun Liu | 2012 | Acta Biochimica et Biophysica Sinica2012，44，10： | 19 |
| 6 | Biological characteristics of an improved triploid crucian carp显示文摘An improved triploid crucian carp(ITCC) was produced by crossing improved tetraploids(G1×AT,) with improved red crucian carp(IRCC,),which were obtained by distant crossing and gynogenesis.The biological characteristics of ITCC,including the number and karyotype of chromosomes,gonadad and pituitary structures,phenotype,and growth rate are reported.ITCC possessed 150 chromosomes with the karyotype 33m+51sm+33st+33t.In the breeding season,both ovary-like and testis-like gonads of ITCC were unable to produce normal mature gametes.The ultrastructure of the pituitary of ITCC showed that most of the endocrine granules in gonadotrophic hormone(GTH) cells had not been released,providing endocrinological evidence for the sterility of ITCC.Compared with triploid crucian carp(TCC) produced by mating Japanese crucian carp with allotetraploid hybrids,ITCC not only retained the excellent traits of fast growth rate and sterility,but also acquired improved morphological characteristics,including higher body,shorter tail and smaller head. | CHEN Song,WANG Jing,LIU ShaoJun,QIN QinBo,XIAO Jun,DUAN Wei,LUO KaiKun,LIU JinHui & LIU Yun Key Laboratory of Protein Chemistry and Developmental Biology of State Education Ministry of China,College of Life Sciences,Hunan Normal University,Changsha 410081,China | 2009 | Science China(Life Sciences)2009，52，8： | 14 |
| 7 | Comparative studies on histological and ultra-structure of the pituitary of different ploidy level fishes显示文摘The histological and ultra-structure of the pituitary in diploid red crucian carp(Carassius auratus red var.),triploid crucian carp and allotetraploid hybrids within and after the breeding season were comparatively studied.The result showed that there were six endocrine cell types in the pituitary of these three kinds of fishes,and there was an obvious difference in cell size among different ploidy level fishes.As for the same type of pituitary cells,the cell size was increased gradually with the in- creasing ploidy level.In the breeding season,the allotetraploid hybrids had higher proportion of go- nadotropin cells(GTH)than triploids,and the triploids had higher proportion of GTH than diploids.The results were related to the earlier sexual maturity of allotetraploid hybrids and sterility of triploid cru- cian carp.On the other hand,among the three kinds of fishes,the proportion of somatotropin(STH) cells in triploids crucian carp was the highest,whereas that in allotetraploid hybrids was the lowest. The results might be connected with the faster growth rate of triploids and slower growth rate of al- lotetraploid hybrids.In addition,in GTH cells of meso-adenohypophysis after the breeding season, there were many endocrine particles in triploids,while those endocrine particles were released from the cells in allotetraploids and diploids.This result showed that the sterility of triploid crucian carp might be related to the hormone which was not released from the GTH cells.In a word,the present study indicated that the differences in the structure of pituitary among different ploidy level fishes contributed to their difference in the growth rate and gonadal development. | LONG Yu LIU Shaojun HUANG Weiren ZHANG Jian SUN Yuandong ZHANG Chun CHEN Song LIU Jinghui LIU Yun | 2006 | Science China(Life Sciences)2006，49，5： | 13 |
| 8 | The cloning of Dmc1 cDNAs and a comparative study of its expression in different ploidy cyprinid fishes显示文摘Dmc1 (disrupted meiotic cDNA) is a functionally specific gene, which was firstly discovered in yeast and then found to encode a protein required for homologous chromosome synapsis during the process of meiosis. In this investigation, we cloned the partial cDNAs of Dmc1 of diploid red crucian carp, Japanese crucian carp, common carp, triploid crucian carp and allotetraploid hybrids by using a pair of degenerate primers based on the conservative sequence of amino acids of the DMC1 protein in yeast, mouse and human. The full length cDNAs were then obtained by rapid amplification of cDNA ends (RACE). Our data showed that the full length cDNAs of Dmc1 in the three diploid fishes are all 1375 bp long, while it is 1383 bp long in triploids and 1379 bp long in allotetraploids. And despite of the variation in length, all the cDNAs encode a protein of 342 amino acids. A high homology of 97.3% of the DMC1 protein can be drawn by comparing the amino acid sequences in the three diploids, which is also of 86%, 86% and 95% similarity to human, mouse and zebrafish, respectively. A comparative study of the expression pattern of Dmc1 was carried out by RT-PCR using specific primers against the same se-quences of coding regions in different ploidy cyprinid fishes, from which it was showed that Dmc1 was expressed only in gonads of these five kinds of fishes. The expression pattern of Dmc1 in both ovaries and testes from different ploidy fishes within breeding season was also studied by Real-time PCR, and the results showed that the expression of this gene was greatly different among the three different ploidy fishes, which was the highest of triploid and lowest of allotetraploids. The histological sections data showed matured gonads of both diploid red crucian carp and allotetraploids in breeding season, although the latter demonstrated a higher maturation, and no gonadal maturation could be observed in triploids. In conclusion, we suggest that Dmc1 is specifically expressed in the period of meiosis in all the ploidy cyprinid fishes and directly related with the development of gonad in a manner of ploidy-independent way. And further, the high expression of Dmc1 in female triploids might be associ-ated with abnormal meiosis and sterility. | TAO Min, LIU ShaoJun, LONG Yu, ZENG Chen, LIU JiFang, LIU LiangGuo, ZHANG Chun, DUAN Wei & LIU Yun Key Laboratory of Protein Chemistry and Developmental Biology of State Education Ministry of China, College of Life Sciences, Hunan Normal University, Changsha 410081, China | 2008 | Science China(Life Sciences)2008，51，1： | 13 |
| 9 | Research advances in animal distant hybridization显示文摘Distant hybridization refers to crosses between two different species or higher-ranking taxa that enables interspecific genome transfer and leads to changes in phenotypes and genotypes of the resulting progeny.If progeny derived from distant hybridization are bisexual and fertile,they can form a hybrid lineage through self-mating,with major implications for evolutionary biology,genetics,and breeding.Here,we review and summarize the published literature,and present our results on fish distant hybridization.Relevant problems involving distant hybridization between orders,families,subfamilies,genera,and species of animals are introduced and discussed,with an additional focus on fish distant hybrid lineages,genetic variation,patterns,and applications.Our review serves as a useful reference for evolutionary biology research and animal genetic breeding. | ZHANG ZhuoHui CHEN Jie LI Ling TAO Min ZHANG Chun QIN QinBo XIAO Jun LIU Yun LIU ShaoJun | 2014 | Science China(Life Sciences)2014，57，9： | 11 |
| 10 | Rapid growth and sterility of growth hormone gene transgenic triploid carp显示文摘Triploid carp(100%)with 150(3n=150)chromosomes were obtained by crossing the females of improved tetraploid hybrids(♀, 4n=200)of red crucian carp(♀)×common carp(♂)with the males of diploid yellow river carp(♂,2n=100).The crosses yielded transgenic triploid carp(positive triploid fish,44.2%of the progeny)and non-transgenic triploid carp(negative triploid fish). Histological examination of the gonads of 24-month-old positive triploid fish suggested they were sterile and the fish were not able to produce mature gametes during the breeding season.Morphologically,both the positive and negative triploid fish were similar.They had a spindle-shaped,laterally compressed,steel grey body with two pairs of barbells.Most of the quantifiable traits of the triploid carp were intermediate between those of the two parents.The positive and negative triploid fish were raised in the same pond for 2 years.The mean body weight of the positive triploid fish was 2.3 times higher than the negative triploid fish.The weight of the largest positive triploid fish was 2.91 times higher than that of the largest negative triploid fish.Thus,we produced fast-growing transgenic triploid carp that have a reduced ecological risk because of their inability to mate and produce progeny. | YU Fan XIAO Jun LIANG XiangYang LIU ShaoJun ZHOU GongJian LUO KaiKun LIU Yun HU Wei WANG YaPing ZHU ZuoYan | 2011 | Chinese Science Bulletin2011，56，16： | 11 |
| 11 | Impact of one satellite outage on ARAIM depleted constellation configurations显示文摘Advanced Receiver Autonomous Integrity Monitoring(ARAIM) is a new technology that will provide worldwide coverage of vertical guidance in aviation navigation. The ARAIM performance and improvement under depleted constellations is a practical problem that needs to be faced and researched further. It is a shortcut that improves the availability in position domain whose key idea is to replace the conventional least squares process with a non-least-squares estimator to lower the integrity risk in exchange for a slight increase in nominal position error. The contributions given in this paper include two parts: First, the impacts of one satellite outage on different constellations are analyzed and compared. The conclusion is that GPS is more sensitive and vulnerable to one satellite outage. Second, a constellation weighted ARAIM(CW-ARAIM)position estimator is proposed. The position solution is replaced by a constellation weighted average solution to eliminate the constellation difference. The new solution will move close to the constellation solutions with respect to the accuracy requirement. The simulation results under baseline GPS and Galileo dual-constellation show that the one GPS satellite outage will knock the availability from 91% to only 50%. The performance remains stable with one Galileo satellite outage. With the assistance of the CW-ARAIM method, the availability can increase from 50% to more than80% under depleted GPS configurations. Even without any satellite outage, the proposed method can effectively improve the availability from 91.29% to 98.75%. The test results under optimistic constellations further verify that a balanced constellation is more important than more satellites on orbit and the superiority of CW-ARAIM method is still effective. | Qian MENG Jianye LIU Qinghua ZENG Shaojun FENG Rui XU | 2019 | Chinese Journal of Aeronautics2019，32，4： | 11 |
| 12 | Key Techniques of High-voltage and Large-capacity Series Hybrid Active Power Filters显示文摘基于基波磁通补偿的串联混合型有源电力滤波器(active power filter,APF)应用于高电压、大容量的谐波抑制场合时,受变压器励磁支路谐波电压的影响,逆变器不能等效为基波电流源,其输出电流将含有大量的谐波,从而影响滤波效果。以逆变器为核心,推导出在励磁支路谐波压降影响下的串联变压器的谐波等效阻抗。并以此为基础,从理论上分析励磁电感和变比对串联变压器谐波等效阻抗和APF滤波效果的影响。仿真结果验证了理论分析的正确性。最后,针对广东某厂10kV、1MVA电力负荷,研制一套基于基波磁通补偿的串联混合型有源滤波器工程样机,取得非常好的滤波效果。 | LIU Jianben CHEN Qiaofu DAI Shaojun HE Guanjin | 2013 | 中国电机工程学报2013，33，12： | 10 |
| 13 | Induction of gynogenesis in red crucian carp using spermatozoa of blunt snout bream显示文摘Gynogenetic 双在红鲫鱼鲤鱼(RCC )(Carassius auratus 红变化) 被导致从钝猪嘴鲷(B)(Megalabrama amblycephala ) 或从镜子鲤鱼(C) 的鸡蛋使用紫外照耀精子(Cyprinus carpio。L) 。精子被适当紫外剂量遗传上使失去活性,然后母亲的 DNA 在 0-4 ° C 与冷吃惊被复制。当使用 B 的精子时,起初喂的授精率,孵化的率和幸存是 52.6 ± 3.0 %, 23.6 ± 4.1 %和 15.7 ± 3.4 %分别地,并且起初喂的幸存比那显著地高( 11.3 ± 2.2%)当使用 C 的精子时( Cypripnus carpio 。L) 。根据词法特征,染色体数字和 gonadal 开发的度, gynogenetic RCC 能与 RCC ♀
的控制混血儿被区分开来B♂ 。有红身体颜色, 100 个染色体和正常 gonadal 开发的个人是 syccessful gyogenetic RCC,当有 124 或 148 个染色体和推迟的 gonadal 开发的个人是混血儿时(RCC B ) 。三倍性的混血儿(RCC B )(2 岁) 是无菌的,但是 tetraploid 混血儿(RCC B ) 是二的性成熟的年龄。在现在的学习,与 C 的精子相比, B 的 spermatoxoa 的优点作为激活来源是那能增加起初 gynogentic 个人和 simplfy 喂 gynogenetic 的证实的幸存地位,它建议 B 的精子是为在鲫鱼鲤鱼导致 gynogenesis 的有效激活来源。 | SUN Yuandong TAO Min LIU Shaojun ZHANG Chun DUAN Wei SHEN Jiamin WANG Jing ZENG Chen LONG Yu LIU Yun | 2007 | Progress in Natural Science:Materials International2007，17，2： | 10 |
| 14 | Almost simple groups with socle ^3D_4(q)act on finite linear spaces显示文摘After the classification of flag-transitive linear spaces,attention has now turned to line-transitive linear spaces.Such spaces are first divided into the point-imprimitive and the point-primitive,the first class is usually easy by the theorem of Delandtsheer and Doyen.The primitive ones are now subdivided,according to the O'Nan-Scotte theorem and some further work by Camina,into the socles which are an elementary abelian or non-abelian simple.In this paper,we consider the latter.Namely,T ≤ G ≤ Aut(T) and G acts line-transitively on finite linear spaces,where T is a non-abelian simple.We obtain some useful lemmas.In particular,we prove that when T is isomorphic to 3D4(q),then T is line-transitive,where q is a power of the prime p. | LIU Weijun, DAI Shaojun & GONG Luozhong School of Mathematics, Central South University, Changsha 410075, China Department of Mathematics, Hunan University of Science and Engineering, Yongzhou 425006, China | 2006 | Science China Mathematics2006，49，12： | 8 |
| 15 | Formation and biological characterization of three new types of improved crucian carp显示文摘The improved tetraploids (G1×AT) were obtained by distant crossing and gynogenesis and the high-body individuals accounted for 2% among G1×AT. After mating with each other, the high-body in- dividuals produced three kinds of bisexual fertile diploid fishes: high-body red crucian carp, high-body fork-like-tails goldfish and gray common carp. The high-body red crucian carp mating with each other formed three types of improved crucian carp (ICC) including improved red crucian carp (IRCC), im- proved color crucian carp (ICCC) and improved gray crucian carp (IGCC). The phenotypes, chromo- some numbers, gonadal structure and fertility of the three kinds of ICC and their offspring were observed. All the three kinds of ICC possessed some improved phenotypes such as higher body, smaller head and shorter tail. The ratios of the body height to body length of IRCC, ICCC and IGCC were 0.54, 0.51 and 0.54, respectively. All of them were obviously higher than that of red crucian carp 0.41 (P<0.01). Three kinds of ICC had the same chromosome number as red crucian carp with 100 chromosomes. All the ICC possessed normal gonads producing mature eggs or sperm, which was important for the production of an improved diploid population. Compared with red crucian carp, all the ICC had stronger fertility such as higher gametes production, higher fertilization rate and higher hatchery rate. Three types of improved diploid fish population were generated from the three kinds of ICC by self-crossing, respectively. The ICC can serve as ornamental fish and edible fish. They are also ideal parents to produce triploids by mating with tetraploids. The new ICC plays an important role in biological evolution and fish genetic breeding. | WANG Jing, QIN QinBo, CHEN Song, LIU ShaoJun, DUAN Wei, LIU JinHui, ZHANG Chun, LUO KaiKun, XIAO Jun & LIU Yun Key Laboratory of Protein Chemistry and Developmental Biology of Ministry of Education of China, College of Life Sciences, Hunan Normal University, Changsha 410081, China | 2008 | Science China(Life Sciences)2008，51，6： | 8 |
| 16 | A hybrid multi-objective PSO algorithm with local search strategy for VLSI partitioning显示文摘 | Wenzhong GUO Genggeng LIU Guolong CHEN Shaojun PENG | 2014 | Frontiers of Computer Science2014，8，2： | 8 |
| 17 | A high-quality genome sequence of alkaligrass provides insights into halophyte stress tolerance显示文摘Alkaligrass(Puccinellia tenuiflora) is a monocotyledonous halophytic forage grass widely distributed in Northern China. It belongs to the Gramineae family and shares a close phylogenetic relationship with the cereal crops, wheat and barley. Here, we present a high-quality chromosome-level genome sequence of alkaligrass assembled from Illumina, Pac Bio and 10× Genomics reads combined with genome-wide chromosome conformation capture(Hi-C) data. The ~1.50 Gb assembled alkaligrass genome encodes 38,387 protein-coding genes, and 54.9% of the assembly are transposable elements, with long terminal repeats being the most abundant. Comparative genomic analysis coupled with stress-treated transcriptome profiling uncovers a set of unique saline-and alkaline-responsive genes in alkaligrass. The high-quality genome assembly and the identified stress related genes in alkaligrass provide an important resource for evolutionary genomic studies in Gramineae and facilitate further understanding of molecular mechanisms underlying stress tolerance in monocotyledonous halophytes. The alkaligrass genome data is freely available at http://gffzz284c18d2de364501h5u0xq0p0kovk69p9.ffgz.tsg.suse.edu.cn/data/alkaligrass.html. | Wenting Zhang Jie Liu Yongxue Zhang Jie Qiu Ying Li Baojiang Zheng Fenhong Hu Shaojun Dai Xuehui Huang | 2020 | Science China(Life Sciences)2020，63，9： | 8 |
| 18 | Polyploid organisms显示文摘Polyploids are organisms with three or more complete chromosome sets. Polyploidization is widespread in plants and animals, and is an important mechanism of speciation. Genome sequencing and related molecular systematics and bioinformatics studies on plants and animals in recent years support the view that species have been shaped by whole genome duplication during evolution. The stability of polyploids depends on rapid genome recombination and changes in gene expression after formation. The formation of polyploids and subsequent diploidization are important aspects in long-term evolution. Polyploids can be formed in various ways. Among them, hybrid organisms formed by distant hybridization could produce unreduced gametes and thus generate offspring with doubled chromosomes, which is a fast, efficient method of polyploidization. The formation of fertile polyploids not only promoted the interflow of genetic materials among species and enriched the species diversity, but also laid the foundation for polyploidy breeding. The study of polyploids has both important theoretical significance and valuable applications. The production and application of polyploidy breeding have brought remarkable economic and social benefits. | SONG Can LIU ShaoJun XIAO Jun HE WeiGuo ZHOU Yi QIN QinBo ZHANG Chun LIU Yun | 2012 | Science China(Life Sciences)2012，55，4： | 7 |
| 19 | Applications of Three DNA Barcodes in Assorting Intertidal Red Macroalgal Flora in Qingdao, China显示文摘This study is part of the endeavor to construct a comprehensive DNA barcoding database for common seaweeds in China. Identifications of red seaweeds, which have simple morphology and anatomy, are sometimes difficult solely depending on morphological characteristics. In recent years, DNA barcode technique has become a more and more effective tool to help solve some of the taxonomic difficulties. Some DNA markers such as COI (cytochrome oxidase subunitⅠ) are proposed as standardized DNA barcodes for all seaweed species. In this study, COI, UPA (universal plastid amplicon, domain V of 23S rRNA), and ITS (nuclear internal transcribed spacer) were employed to analyze common species of intertidal red seaweeds in Qingdao (119.3 -121 E, 35.35 -37.09 N). The applicability of using one or a few combined barcodes to identify red seaweed species was tested. The results indicated that COI is a sensitive marker at species level. However, not all the tested species gave PCR amplification products due to lack of the universal primers. The second barcode UPA had effective universal primers but needed to be tested for the effectiveness of resolving closely related species. More than one ITS sequence types were found in some species in this investigation, which might lead to confusion in further analysis. Therefore ITS sequence is not recommended as a universal barcode for seaweeds identification. | ZHAO Xiaobo PANG Shaojun SHAN Tifeng LIU Feng | 2013 | Journal of Ocean University of China2013，12，1： | 7 |
| 20 | Human natural killer cells for targeting delivery of gold nanostars and bimodal imaging directed photothermal/photodynamic therapy and immunotherapy显示文摘Objective:To construct a novel nanoplatform GNS@CaCO3/Ce6-NK by loading the CaCO3-coated gold nanostars(GNSs)with Chlorin e6 molecules(Ce6)into human peripheral blood mononuclear cells(PBMCs)-derived NK cells for tumor targeted therapy.Methods:GNS@CaCO3/Ce6 nanoparticles were prepared and characterized by TEM and UV-vis.The cell surface markers and cytokines secretion of NK cells before and after loading the GNS@CaCO3/Ce6 nanoparticles were detected by Flow Cytometry(FCM)and ELISA.Effects of the GNS@CaCO3/Ce6-NK cells on A549 cancer cells was determined by FCM and CCK-8.Intracellular fluorescent signals of GNS@CaCO3/Ce6-NK cells were detected via Confocal laser scanning microscopic(CLSM)and FCM at different time points.Intracellular ROS generation of GNS@CaCO3/Ce6-NK cells under laser irradiation were examined by FCM.The distribution of GNS@CaCO3/Ce6-NK in A549 tumor-bearing mice were observed by fluorescence imaging and PA imaging.The combination therapy of GNS@CaCO3/Ce6-NK under laser irradiation were investigated on tumor-bearing mice.Results:The coated CaC03 shell on the surface of GNSs exhibited prominent delivery and protection effect of Ce6 during the cellular uptake process.The as-prepared multifunctional GNS@CaCO3/Ce6-NK cells possessed bimodal functions of fluorescence imaging and photoacoustic imaging.The as-prepared multifunctional GNS@CaCO3/Ce6-NK cells could actively target tumor tissues with the enhanced photothermal/photodynamic therapy and immunotherapy.Conclusions:The GNS@CaCO3/Ce6-NK shows effective tumor-targeting ability and prominent therapeutic efficacy toward lung cancer A549 tumor-bearing mice.Through fully utilizing the features of GNSs and NK cells,this new nanoplatform provides a new synergistic strategy for enhanced photothermal/photodynamic therapy and immunotherapy in the field of anticancer development in the near future. | Bin Liu Wen Cao Jin Cheng Sisi Fan Shaojun Pan Lirui Wang Jiaqi Niu Yunxiang Pan Yanlei Liu Xiyang Sun Lijun Ma Jie Song Jian Ni Daxiang Cui | 2019 | Cancer Biology & Medicine2019，16，4： | 7 |
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