|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Multi-spectroscopic characterization of bovine serum albumin upon interaction with atomoxetine显示文摘The quenching interaction of atomoxetine(ATX) with bovine serum albumin(BSA) was studied in vitro under optimal physiological condition(pH=7.4) by multi-spectroscopic techniques. The mechanism of ATX-BSA system was a dynamic quenching process and was confirmed by the fluorescence spectra and lifetime measurements. The number of binding sites, binding constants and other binding characteristics were computed. Thermodynamic parameters ΔH^0 and ΔS^0 indicated that intermolecular hydrophobic forces predominantly stabilized the drug-protein system. The average binding distance between BSA and ATX was studied by F?rsters theory. UV-absorption, Fourier transform infrared spectroscopy(FT-IR), circular dichroism(CD), synchronous spectra and three-dimensional(3D) fluorescence spectral results revealed the changes in micro-environment of secondary structure of protein upon the interaction with ATX. Displacement of site probes and the effects of some common metal ions on the binding of ATX with BSA interaction were also studied. | Arunkumar T. Buddanavar Sharanappa T. Nandibewoor | 2017 | Journal of Pharmaceutical Analysis2017，7，3： | 10 |
| 2 | Multi-spectroscopic investigation of the binding interaction of fosfomycin with bovine serum albumin显示文摘The interaction between fosfomycin(FOS) and bovine serum albumin(BSA) has been investigated effectively by multi-spectroscopic techniques under physiological p H 7.4. FOS quenched the intrinsic fl uorescence of BSA via static quenching. The number of binding sites n and observed binding constant K A were measured by the fl uorescence quenching method. The thermodynamic parameters ΔG0, ΔH0andΔS0were calculated at different temperatures according to the van't Hoff equation. The site of binding of FOS in the protein was proposed to be Sudlow's site I based on displacement experiments using site markers viz. warfarin, ibuprofen and digitoxin. The distance r between the donor(BSA) and acceptor(FOS) molecules was obtained according to the F?rster theory. The effect of FOS on the conformation of BSA was analyzed using synchronous fl uorescence spectra(SFS), circular dichroism(CD) and 3D fl uorescence spectra. A molecular modeling study further con fi rmed the binding mode obtained by the experimental studies. | Manjunath D.Meti Sharanappa T.Nandibewoor Shrinivas D.Joshi Uttam A.More Shivamurti A.Chimatadar | 2015 | Journal of Pharmaceutical Analysis2015，5，4： | 3 |
| 3 | Regio selective butylation of toluene on mordenite catalysts influence of acidity显示文摘 | Sebastian C P Shivanand P Sharanappa N | 2004 | J Mol Catal A:Chem2004，223，12： | 1 |
| 4 | Regio selective butylation of toluene on mordenite catalysts influence of acidity显示文摘 | Sebastian C P Shivanand Pai Sharanappa N | 2004 | Journal of Molecular Catalysis A:Chemical2004，223，： | 1 |
| 5 | Fabrication of multiwalled carbon nanotube-surfactant modi fi ed sensor for the direct determination of toxic drug 4-aminoantipyrine显示文摘A multi-walled carbon nanotube(MWCNT)-cetyltrimethylammonium bromide(CTAB) surfactant composite modi fi ed glassy carbon electrode(GCE) was developed as a novel system for the determination of4-aminoantipyrine(AAP). The oxidation process was irreversible over the p H range studied and exhibited a diffusion controlled behavior. All experimental parameters were optimized. The combination of MWCNT-CTAB endows the biosensor with large surface area, good biological compatibility, electricity and stability, high selectivity and sensitivity. MWCNT-CTAB/GCE electrode gave a linear response for AAP from 5.0 109 to 4.0 108 M with a detection limit of 1.63 1010 M. The modi fi ed electrode showed good selectivity against interfering species and also exhibited good reproducibility. The present electrochemical sensor based on the MWCNT-CTAB/GCE electrode was applied to the determination of AAP in real samples. | Jayant I.Gowda Arunkumar T.Buddanavar Sharanappa T.Nandibewoor | 2015 | Journal of Pharmaceutical Analysis2015，5，4： | 1 |
| 6 | Transparent Cu 1.8 S and CuS thin films on FTO as efficient counter electrode for quantum dot solar cells显示文摘 | Shankara Sharanappa Kalanur Sang Youn Chae Oh Shim Joo | 2013 | Electrochimica Acta2013，，： | 1 |
| 7 | Electro-oxidation and Determination of Trazodone at Multiwalled Carbon Nanotube-modified Glassy Carbon Electrode显示文摘 | Rajesh N Hegde Nagaraj P Shetti Sharanappa T Nandibewoor | 2009 | Talanta2009，79，： | 1 |
| 8 | Non-covalent binding analysis of sulfamethoxazole to human serum albumin:Fluorescence spectroscopy,UV-vis,FT-IR,voltammetric and molecular modeling显示文摘This study was designed to examine the interaction of sulfamethoxazole(SMZ) with human serum albumin(HSA).Spectroscopic analysis of the emission quenching at different temperatures revealed that the quenching mechanism of human serum albumin by SMZ was static mechanism.The binding constant values for the SMZ-HSA system were obtained to be 22,500 L/mol at 288 K,15,600 L/mol at 298 K,and 8500 L/mol at 308 K.The distance r between donor and acceptor was evaluated according to the theory of Foster energy transfer.The results of spectroscopic analysis and molecular modeling techniques showed that the conformation of human serum albumin had been changed in the presence of SMZ.The thermodynamic parameters,namely enthalpy change(△H^0)-36.0 kJ/mol,entropy change(△S^0)-41.3 J/mol K and free energy change(△G^0)-23.7 kJ/mol,were calculated by using van't Hoff equation.The effect of common ions on the binding of SMZ to HSA was tested. | Praveen N.Naik Sharanappa T.Nandibewoor Shivamurthi A.Chimatadar | 2015 | Journal of Pharmaceutical Analysis2015，5，3： | 1 |
| 9 | Electro-oxidation and determination of trazodone at multiwalled carbon nanotube-modified glassy carbon electrode显示文摘 | Rajesh N Hegde Nagaraj P Shetti Sharanappa T Nandibewoor | 2009 | Talanta2009，79，2： | 1 |
| 10 | Investigation of binding behaviour of procainamide hydrochloride with human serum albumin using synchronous,3D fluorescence and circular dichroism显示文摘Interaction of procainamide hydrochloride(PAH) with human serum albumin(HSA) is of great significance in understanding the pharmacokinetic and pharmacodynamic mechanisms of the drug. Multi-spectroscopic techniques were used to investigate the binding mode of PAH to HSA and results revealed the presence of static type of quenching mechanism. The number of binding sites, binding constants and thermodynamic parameters were calculated. The results showed a spontaneous binding of PAH to HSA and hydrophobic interactions played a major role. In addition, the distance between PAH and the Trp–214 was estimated employing the F?rster's theory. Site marker competitive experiments indicated that the binding of PAH to HSA primarily took place in subdomain IIA(Sudlow's site I). The influence of interference of some common metal ions on the binding of PAH to HSA was studied. Synchronous fluorescence spectra(SFS), 3D fluorescence spectra and circular dichroism(CD) results indicated the conformational changes in the structure of HSA. | Kirthi Byadagi Manjunath Meti Sharanappa Nandibewoor Shivamurti Chimatadar | 2017 | Journal of Pharmaceutical Analysis2017，7，2： | 1 |
| 11 | Regio selective butylation of toluene on mordenite catalysts influence of acidity 显示文摘 | Sebastian C P Shivanand P Sharanappa N | 2004 | J Mol Catal A : Chem2004，223，12： | 1 |
| 12 | Electro-oxidation and determination of antihistamine drug,cetirizine dihydrochloride at glassy carbon electrode modified with multiwalled carbon nanotubes显示文摘 | Roopa H Patil Rajesh N Hegde Sharanappa T | | 0，，： | 1 |
| 13 | Electrochemical behavior of paclitaxel and its determination at glassy carbon electrode显示文摘The electrochemical behavior of paclitaxel drug was studied at a glassy carbon electrode in phosphate buffer solutions using cyclic and differential-pulse voltammetric techniques.The oxidation process was shown to be irreversible over the pH range(3.0e10.4)and was diffusion controlled.Effects of anodic peak potential(E_(p)),anodic peak current(Ipa),scan rate,pH,heterogeneous rate constant(k^(0)),etc have been discussed.A possible electrooxidation mechanism was proposed.An analytical method was developed for the determination of paclitaxel in phosphate buffer solution at pH¼7.0 as a supporting electrolyte.The anodic peak current varied linearly with paclitaxel concentration in the range 1.0×10^-(6)M to 1.0×10^-(5)M with a limit of detection(LOD)of 1.23×10^(-8)M and limit of quantification(LOQ)of 4.10×10^(-8)M.The proposed method was successfully applied to the determination of paclitaxel in pure and real samples. | Jayant I.Gowda Sharanappa T.Nandibewoor | 2014 | Asian Journal of Pharmaceutical Sciences2014，9，1： | 0 |
您所在的机构暂未开通此部分的功能，请联系管理员