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| 1 | Attenuation of MET-mediated migration and invasion in hepatocellular carcinoma cells by SOCS1显示文摘AIM To investigate the role of suppressor of cytokine signaling 1(SOCS1)in regulating MET-mediated invasive potential of hepatocellular carcinoma(HCC)cells.METHODSStable derivatives of mouse(Hepa1-6)and human(hep3B,Hep G2)HCC cell lines expressing SOCS1or control vector were evaluated for their ability to migrate towards hepatocyte growth factor(HGF)in the transwell migration assay,invade extracellular matrix in response to HGF stimulation in a 3-D invasion assay by confocal microscopy,and to undergo anchorageindependent proliferation in semisolid agar.Following intravenous and intrasplenic inoculation into NOD.scid.gamma mice,the ability of Hepa cells to form othotopic tumors was evaluated.Following HGF stimulation of Hepa and Hep3B cells,expression of proteins implicated in epithelial-to-mesenchymal transition was evaluated by western blot and qR T-PCR.RESULTS SOCS1 expression in mouse and human HCC cells inhibited HGF-induced migration through matrigel.In the 3-D invasion assay,HGF stimulation induced invasion of HCC cells across type-Ⅰcollagen matrix,and SOCS1expression significantly reduced the depth of invasion.SOCS1 expression also reduced the number and size of colonies formed by anchorage-independent growth in semisolid agar.Following intravenous inoculation,control Hepa cell formed large tumor nodules that obliterated the liver whereas the SOCS1-expressing Hepa cells formed significantly smaller nodules.Tumors formed by SOCS1-expressing cells showed reduced phosphorylation of STAT3 and ERK that was accompanied by reduced levels of MET protein expression.HGF stimulated Hepa cells expressing SOCS1 showed increased expression of E-cadherin and decreased expression of EGR1,SNAI1and ZEB1.Comparable results were obtained with Hep3B cells.SOCS1 expressing HCC cells also showed reduced levels of EGR1 and SNAI1 transcripts.CONCLUSION Our findings indicate that loss of SOCS1-dependent control over epithelial-to-mesenchymal transition may contribute to MET-mediated migration,invasion and metastatic growth of HCC. | Yirui Gui Md Gulam Musawwir Khan Diwakar Bobbala Claire Dubois Sheela Ramanathan Caroline Saucier Subburaj Ilangumaran | 2017 | World Journal of Gastroenterology2017，23，36： | 3 |
| 2 | PCV2疫苗和免疫接种的10年:净化能否成为事实显示文摘在猪圆环病毒2型(Porcine Circovirus type 2,PCV2)出现后的20多年,它仍然是一种对养猪业有重要经济影响的猪病。PCV2的商用疫苗在2006年首次进入美国市场,在减少感染猪的临床症状和提高产量方面起到了显著的作用。最近的研究表明,PCV2在猪场猪群中的流行性和败血症发生率有所下降。然而,新变异毒株的出现又使PCV2的流行性上升。本文将评论PCV2疫苗领域最新热点的话题,包括比较商用疫苗的免疫效果、现有疫苗对新型毒株的免疫效力、自然感染和接种疫苗在免疫力上的差异、目前PCV2疫苗研究模型的局限性以及新型试验疫苗的研究新进展。讨论也是在未来PCV2可得到净化的前提下进行的。 | 刘国民(译) 宋庆庆(校) 林文耀(校) Zahra Afghaha Brett Webbb Xiang-Jin Mengc Sheela Ramamoorthy | 2019 | 国外畜牧学(猪与禽)2019，39，9： | 2 |
| 3 | Trans-presentation of interleukin-15 by interleukin-15 receptor alpha is dispensable for the pathogenesis of autoimmune type 1 diabetes显示文摘Interleukin-15(IL-15)is a pro-inflammatory cytokine that is required for the survival and activation of memory CD8^(+)T cells,natural killer(NK)cells,innate lymphoid cells,macrophages and dendritic cells.IL-15 is implicated in the pathogenesis of various autoimmune diseases such as rheumatoid arthritis,inflammatory bowel disease,psoriasis and autoimmune type 1 diabetes(T1D).IL-15 receptor(IL-15R)consists of a specificαchain,theβchain that is shared with IL-2R and the commonγchain.IL-15 is unique in the manner in which it binds and signals through its receptor subunits.IL-15 that is complexed with IL-15Rαbinds to theβγreceptor complex present on the responding cell to mediate its biological effects through a process referred to as trans-presentation.The trans-presented IL-15 is essential to mediate the biological effects on T lymphocytes and NK cells.Here we show that IL-15,but not IL-15Rα,is required for the development of spontaneous and virus-induced T1D,viral clearance and for antigen cross-presentation to CD8^(+)T lymphocytes.Our findings provide insight into the complexities of IL-15 signalling in the initiation and maintenance of CD8^(+)T cell-mediated immune responses. | Diwakar Bobbala Marian Mayhue Alfredo Menendez Subburaj Ilangumaran Sheela Ramanathan | 2017 | Cellular & Molecular Immunology2017，14，7： | 2 |
| 4 | The Resort Cycle and Seaside Tourism: an Assessment of its Applicability and Validity显示文摘 | Sheela Agarwal | 1997 | Tourism management1997，18，2： | 1 |
| 5 | Effect of cadmium and zinc interaction on metal uptake and regenerate on tolerant plant sinin seed显示文摘 | Sheela S | 1997 | Agriculture Ecosystems and Environment1997，61，： | 1 |
| 6 | Direct electron transfer with yeast cells and construction of a mediatorless microbial fuel cell显示文摘 | D. Prasad S. Arun M. Murugesan S. Padmanaban R.S. Satyanarayanan Sheela Berchmans V. Yegnaraman | 2006 | Biosensors and Bioelectronics2006，，11： | 1 |
| 7 | Biosorption of chromium ( Ⅵ ) by free and immobilized rhizopus arrhizus显示文摘 | Prakasham R S Merrie J S Sheela R | 1999 | Environmental Pollution1999，104，3： | 1 |
| 8 | Epoxidation and oxidation reactions using 1,4-butanediol dimethacrylate crosslinked polystyrene-supported tertiary butyl hydroperoxide显示文摘 | Sheela M S Sreekumar K | 2004 | J Chem Sci2004，116，6： | 1 |
| 9 | Chemotherapy for Operable Gastric Cancer: Current Perspectives显示文摘 | Vikram K. Jain David Cunningham Sheela Rao | 2011 | Indian Journal of Surgical Oncology2011，，4： | 1 |
| 10 | Anti-diabetic effects of onion and garlic sulfoxide amino acids in rals显示文摘 | Sheela C G Kumari K Augusti K T | 1995 | Planta-MEDICA1995，61，4： | 1 |
| 11 | Anti- diabetic effects of onion and garlic sulfoxide amino acids in rats显示文摘 | Sheela C G Kumari K Augusti K T | 1995 | Planta-Medica1995，61，4： | 1 |
| 12 | Biosorption of chromium Ⅵ by free and immobilized Rhizopus arrhizus 显示文摘 | Prakasham R S Merrie J S Sheela R Saswathi N Ramakrishna S V | 1999 | Environmental Pollution1999，104，3： | 1 |
| 13 | Diamond-dispersed electroless nickel coatings 显示文摘 | Sheela G Pushpavanam M | 2002 | Metal Finishing2002，100，1： | 1 |
| 14 | Appearance of hepatocellular adenomas on gadoxetic acid-enhanced MRI显示文摘 | Timm Denecke Ingo G. Steffen Sheela Agarwal Daniel Seehofer Thomas Kr?ncke Enrique Lopez H?nninen Incken-Birthe Kramme Peter Neuhaus Sanjay Saini Bernd Hamm Christian Grieser | 2012 | European Radiology2012，，8： | 1 |
| 15 | Synthesis and studies on s-triazine-based ligand and its metal complexes显示文摘 | Shanmugakala R Tharmaraj P Sheela C D | 2012 | International Journal of Inor- ganic Chemistry2012，2012，： | 1 |
| 16 | Upper GI mucosal effects of parecoxib sodium in healthy elderly subjects显示文摘 | Randall R Stoltz Stuart I Harris Michael E Kuss Diane LeComte Sheela Talwalker Shobha Dhadda Richard C Hubbard | 2002 | The American Journal of Gastroenterology2002，，1： | 1 |
| 17 | Functionalization of electrochemically deposited chitosan films with alginate and prussian blue for enhanced performance of microbial fuel cells显示文摘 | Navanietha Krishnaraj R Karthikeyan R Sheela Berchmans | 2013 | Eleetrochimiea Acta2013，112，： | 1 |
| 18 | Indole-3-acetic acid biosynthesis in the biocontrol strain Pseudomonas fluorescens Psd and plant growth regulation by hormone overexpression显示文摘 | Mandira Kochar Ashutosh Upadhyay Sheela Srivastava | 2011 | Research in Microbiology2011，，4： | 1 |
| 19 | Kinetics and ther- modynamics studies on the adsorption of Zn(Ⅱ) , Cd(Ⅱ) and Hg (Ⅱ) from aqueous solution using zinc oxide nanoparticles 显示文摘 | Sheela T Nayaka Y A Viswanatha R | 2012 | Powder Technology2012，217，： | 1 |
| 20 | Effect of cadmium and zinc interaction on metal uptake and regeneration of tolerant plants in linseed显示文摘 | BIPASHA C SHEELA S | 1997 | Agriculture Ecosystems and Environment1997，61，： | 1 |
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