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| 1 | RBD-Fc-based COVID-19 vaccine candidate induces highly potent SARS-CoV-2 neutralizing antibody response显示文摘The pandemic of coronavirus disease 2019(COVID-19)caused by severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)has posed serious threats to global health and economy,thus calling for the development of safe and effective vaccines.The receptorbinding domain(RBD)in the spike protein of SARS-CoV-2 is responsible for its binding to angiotensin-converting enzyme 2(ACE2)receptor.It contains multiple dominant neutralizing epitopes and serves as an important antigen for the development of COVID-19 vaccines.Here,we showed that immunization of mice with a candidate subunit vaccine consisting of SARS-CoV-2 RBD and Fc fragment of human IgG,as an immunopotentiator,elicited high titer of RBD-specific antibodies with robust neutralizing activity against both pseudotyped and live SARS-CoV-2 infections.The mouse antisera could also effectively neutralize infection by pseudotyped SARS-CoV-2 with several natural mutations in RBD and the IgG extracted from the mouse antisera could also show neutralization against pseudotyped SARS-CoV and SARS-related coronavirus(SARSr-CoV).Vaccination of human ACE2 transgenic mice with RBD-Fc could effectively protect mice from the SARS-CoV-2 challenge.These results suggest that SARS-CoV-2 RBD-Fc has good potential to be further developed as an effective and broad-spectrum vaccine to prevent infection of the current SARS-CoV-2 and its mutants,as well as future emerging SARSr-CoVs and re-emerging SARS-CoV. | Zezhong Liu Wei Xu Shuai Xia Chenjian Gu Xinling Wang Qian Wang Jie Zhou Yanling Wu Xia Cai Di Qu Tianlei Ying Youhua Xie Lu Lu Zhenghong Yuan Shibo Jiang | 2020 | Signal Transduction and Targeted Therapy2020，5，1： | 7 |
| 2 | Mechanical properties and expansion coefficient of Mo-Cu composites with different Ni contents显示文摘Mo-Cu composites were fabricated by powder metallurgy with addition of various Ni contents.The effect of Ni contents on mechanical and thermal properties of Mo-Cu composites was investigated.The results show that mechanical and thermal properties of Mo-Cu composites are greatly affected by the addition of Ni contents.The composite powders with Ni addition exhibits high sinterability.The sintering temperature is greatly decreased and the comprehensive properties of Mo-Cu composites are obviously improved.Mo-Cu composites with a content of 1.5 wt.% Ni have relative density 99.2%,bending strength 1057.9 MPa,hardness 72.5 HRA,electronic resistivity 1.28×10^(-7)Ω·m^(-1),thermal conductivity 139 W.m^(-1).K^(-1),and lower coefficient of thermal expansion 7.4×10^(-6)K^(-1).Mo-Cu composites have homogeneous and fine microstructure.The fracture mechanism is ductile fracture. | Guo, Shibo Kang, Qiping Cai, Chunbo Qu, Xuanhui | 2012 | Rare Metals2012，31，4： | 7 |
| 3 | Design of hierarchical, three-dimensional free-standing single-atom electrode for H2O2 production in acidic media显示文摘Electrochemical reduction of molecular O2 to hydrogen peroxide(H2O2)offers a promising solution for water purification and environmental remediation.Here,we design a hierarchical free-standing single-Co-atom(with Co-N4 coordination)electrode for oxygen reduction reaction(ORR)via a two-electron pathway to make H2O2 in acidic media.The current density of the single-Co-atom electrode reached 51 mA/cm2 at 0.1 V vs reversible hydrogen electrode,lasting for more than 10 hours of continuous operation with H2O2 selectivity greater than 80%.Toward practical application,the single-Co-atom electrode was directly used to assemble an electrochemical cell to produce H2O2 at a rate of 676 mol/kgcat/h with a cell voltage of about 1.6 V. | Jincheng Zhang Hongbin Yang Jiajian Gao Shibo Xi Weizheng Cai Junming Zhang Ping Cui Bin Liu | 2020 | Carbon Energy2020，2，2： | 6 |
| 4 | A highly potent and stable pan-coronavirus fusion inhibitor as a candidate prophylactic and therapeutic for COVID-19 and other coronavirus diseases显示文摘The development of broad-spectrum antivirals against human coronaviruses(HCoVs)is critical to combat the current coronavirus disease 2019(COVID-19)pandemic caused by severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)and its variants,as well as future outbreaks of emerging CoVs.We have previously identified a polyethylene glycol-conjugated(PEGylated)lipopeptide,EK1C4,with potent pan-CoV fusion inhibitory activity.However,PEG linkers in peptide or protein drugs may reduce stability or induce anti-PEG antibodies in vivo.Therefore,we herein report the design and synthesis of a series of dePEGylated lipopeptide-based pan-CoV fusion inhibitors featuring the replacement of the PEG linker with amino acids in the heptad repeat 2 C-terminal fragment(HR2-CF)of HCoV-OC43.Among these lipopeptides,EKL1C showed the most potent inhibitory activity against infection by SARS-CoV-2 and its spike(S)mutants,as well as other HCoVs and some bat SARS-related coronaviruses(SARSr-CoVs)tested.The dePEGylated lipopeptide EKL1C exhibited significantly stronger resistance to proteolytic enzymes,better metabolic stability in mouse serum,higher thermostability than the PEGylated lipopeptide EK1C4,suggesting that EKL1C could be further developed as a candidate prophylactic and therapeutic for COVID-19 and other coronavirus diseases. | Jie Zhou Wei Xu Zezhong Liu Chao Wang Shuai Xia Qiaoshuai Lan Yanxing Cai Shan Su Jing Pu Lixiao Xing Youhua Xie Lu Lu Shibo Jiang Qian Wang | 2022 | Acta Pharmaceutica Sinica B2022，12，4： | 5 |
| 5 | Engineered trimeric ACE2 binds viral spike protein and locks it in'Thfee-up'conformation to potently inhibit SARS-CoV-2 infection显示文摘Dear Editor,Coronavirus disease 2019(COVID-19)caused by severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)has resulted in a severe global pandemic Following SARS-CoV,SARS-CoV-2 is yet another emerge nt beta-coronavirus threate ning human health.1 However,seventeen years after the SARS pandemic,no targeted vaccines or therapeutics have been approved for SARS,while some of them might have held promise for treating COVID-19.Many neutralizing antibodies against SARS-CoV-2 are currently being developed.However,RNA viruses are known to have high mutation rates. | Liang Guo Wenwen Bi Xinling Wang Wei Xu Renhong Yan Yuanyuan Zhang Kai Zhao Yaning Li Mingfeng Zhang Xia Cai Shibo Jiang Youhua Xie Qiang Zhou Lu Lu Bobo Dang | 2021 | Cell Research2021，31，1： | 5 |
| 6 | Griffithsin with A Broad-Spectrum Antiviral Activity by Binding Glycans in Viral Glycoprotein Exhibits Strong Synergistic Effect in Combination with A Pan-Coronavirus Fusion Inhibitor Targeting SARS-CoV-2 Spike S2 Subunit显示文摘Dear Editor,The pandemic of coronavirus disease 2019(COVID-19)caused by SARS-Co V-2 has posed a significant threat to global public health and economy,thus calling for the rapid development of effective therapeutics and prophylactics.Repurposing existing medicines with clinical safety profiles offers a more rapid hope of combating COVID-19pandemic than developing a new therapeutic. | Yanxing Cai Wei Xu Chenjian Gu Xia Cai Di Qu Lu Lu Youhua Xie Shibo Jiang | 2020 | Virologica Sinica2020，35，6： | 4 |
| 7 | Genome Sequence of a Marine Carotenoid Producing Yeast Rhodotorula mucilaginosa CYJ03显示文摘Carotenoids are valuable pigments that have been widely used in food,pharmaceutical,animal breeding and cosmetics industries.Due to the increasing demand for carotenoids of natural origin,the trend for production of carotenoids by red yeast has become popular.Strain Rhodotorula mucilaginosa CYJ03 was isolated from northern Yellow Sea of China for its carotenoid producing potential.It was found that the whole genome of CYJ03 was 19.03 Mb in size and contained 6301 protein-coding genes including a gene cluster for the carotenoids biosynthesis.The genome sequence would be valuable for exploring the potential biological properties of CYJ03,as well as for facilitating the molecular genetic analysis and the manipulation of carotenoids accumulation in this strain,and for the development of it as an engineered host for carotenoid production. | TANG Wei WANG Yue CAI Yali LIU Shibo ZHANG Jun HE Zengguo | 2020 | Journal of Ocean University of China2020，19，2： | 3 |
| 8 | Structural and functional basis for pan-CoV fusion inhibitors against SARS-CoV-2 and its variants with preclinical evaluation显示文摘The COVID-19 pandemic poses a global threat to public health and economy.The continuously emerging SARS-CoV-2 variants present a major challenge to the development of antiviral agents and vaccines.In this study,we identified that EK1 and cholesterol-coupled derivative of EK1,EK1C4,as pan-CoV fusion inhibitors,exhibit potent antiviral activity against SARS-CoV-2 infection in both lung-and intestine-derived cell lines(Calu-3 and Caco2,respectively).They are also effective against infection of pseudotyped SARS-CoV-2 variants B.1.1.7(Alpha)and B.l.1.248(Gamma)as well as those with mutations in S protein,including N417T,E484K,N501Y,and D614G,which are common in South African and Brazilian variants.Crystal structure revealed that EK1 targets the HR1 domain in the SARS-CoV-2 S protein to block virus-cell fusion and provide mechanistic insights into its broad and effective antiviral activity.Nasal administration of EK1 peptides to hACE2 transgenic mice significantly reduced viral titers in lung and intestinal tissues.EK1 showed good safety profiles in various animal models,supporting further clinical development of EK1-based pan-CoV fusion inhibitors against SARS-CoV-2 and its variants. | Shuai Xia Qiaoshuai Lan Yun Zhu Chao Wang Wei Xu Yutang Li Lijue Wang Fanke Jiao Jie Zhou Chen Hua Qian Wang Xia Cai Yang Wu Jie Gao Huan Liu Ge Sun Jan Munch Frank Kirchhoff Zhenghong Yuan Youhua Xie Fei Sun Shibo Jiang Lu Lu | 2021 | Signal Transduction and Targeted Therapy2021，6，8： | 2 |
| 9 | Tumor extracellular vesicles mediate anti-PD-L1 therapyresistance by decoying anti-PD-L1显示文摘PD-L1+tumor-derived extracellular vesicles(TEVs)cause systemic immunosuppression and possibly resistance to anti-PD-L1 antibody(αPD-L1)blockade.However,whether and how PD-L1+TEVs mediateαPD-L1 therapy resistance is unknown.Here,we show that PD-L1+TEVs substantially decoyαPD-L1 and that TEV-boundαPD-L1 is more rapidly cleared by macrophages,causing insufficient blockade of tumor PD-L1 and subsequentαPD-L1 therapy resistance.Inhibition of endogenous production of TEVs by Rab27a or Coro1a knockout reversesαPD-L1 therapy resistance.Either an increasedαPD-L1 dose or macrophage depletion mediated by the clinical drug pexidartinib abolishesαPD-L1 therapy resistance.Moreover,in the treatment cycle with the same total treatment dose ofαPD-L1,high-dose and low-frequency treatment had better antitumor effects than low-dose and highfrequency treatment,induced stronger antitumor immune memory,and eliminatedαPD-L1 therapy resistance.Notably,in humanized immune system mice with human xenograft tumors,both increasedαPD-L1 dose and high-dose and low-frequency treatment enhanced the antitumor effects ofαPD-L1.Furthermore,increased doses ofαPD-L1 andαPD-1 had comparable antitumor effects,butαPD-L1 amplified fewer PD-1+Treg cells,which are responsible for tumor hyperprogression.Altogether,our results reveal a TEV-mediated mechanism ofαPD-L1-specific therapy resistance,thus providing promising strategies to improveαPD-L1 efficacy. | Jiming Chen Jie Yang Wenhui Wang Danfeng Guo Chengyan Zhang Shibo Wang Xinliang Lu Xiaofang Huang Pingli Wang Gensheng Zhang Jing Zhang Jianli Wang Zhijian Cai | 2022 | Cellular & Molecular Immunology2022，19，11： | 1 |
| 10 | MnS hollow microspheres combined with carbon nanotubes for enhanced performance sodium-ion battery anode显示文摘MnS as anode material for sodium-ion batteries(SIBs)has recently attracted great attention because of the high theoretical capacity,great natural abundance,and low cost.However,it suffers from inferior electrical conductivity and large volume expansion during the charge/discharge process,leading to tremendous damage of electrodes and subsequently fast capacity fading.To mitigate these issues,herein,a three-dimensional(3D)interlaced carbon nanotubes(CNTs)threaded into or between MnS hollow microspheres(hollow MnS/CNTs composite)has been designed and synthesized as an enhanced anode material.It can effectively improve the electrical conductivity,buffer the volume change,and maintain the integrity of the electrode during the charging and discharging process based on the synergistic interaction and the integrative structure.Therefore,when evaluated as anode for SIBs,the hollow MnS/CNTs electrode displays enhanced reve rsible capacity(275 mAh/g at 100 mA/g after 100 cycles),which is much better than that of pure MnS electrode(25 mAh/g at 100 mA/g after 100 cycles)prepared without the addition of CNTs.Even increasing the current density to 500 mA/g,the hollow MnS/CNTs electrode still delivers a five times higher reversible capacity than that of the pure MnS electrode.The rate performance of the hollow MnS/CNTs electrode is also superior to that of pure MnS electrode at various current densities from 50 mA/g to 1000 mA/g. | Na Zhang Xin Li Tianyi Hou Jinze Guo Anran Fan Shibo Jin Xiaohong Sun Shu Cai Chunming Zheng | 2020 | Chinese Chemical Letters2020，31，5： | 1 |
| 11 | Anti-SARS-CoV-2 IgY Isolated from Egg Yolks of Hens Immunized with Inactivated SARS-CoV-2 for Immunoprophylaxis of COVID-19显示文摘Dear Editor,In the absence of vaccine,the mounting second wave of COVID-19 infections worldwide calls for the rapid development of human neutralizing antibodies to prevent and treat COVID-19(Lee et al.2020).Considering the high cost and long development cycle of human neutralizing antibodies,we turned to anti-SARS-Co V-2 Ig Y for cheap and fast scale up. | Haiyan Shen Yanxing Cai Huan Zhang Jie Wu Lin Ye Penghui Yang Xiaojun Lin Shibo Jiang Ming Liao | 2021 | Virologica Sinica2021，36，5： | 1 |
| 12 | Accurate characterization of bubble mixing uniformity in a circular region using computational geometric theory显示文摘The present study proposes a novel method based on the geometric theory for measuring the distribution of bubble swarms in the circular region of a direct-contact heat exchanger.It was determined that the mixing is uniform when the average distance between the bubble swarms in the unit circular region is approximately 0.9054,which is the standard reference value.The effect of sample size(i.e.,the number of bubbles)on mixing uniformity was investigated to determine the optimal sample size.It was verified that the metric's accuracy and stability were higher with a sample size of 155.Accordingly,it was proposed to increase the sample size by filling irregular bubbles using a segmentation method,which enabled a further accurate assessment of the mixing uniformity.The mixing uniformity of bubble swarms in the circular region and its maximum internal connection with the square region was accurately quantified.It was revealed that the relative average error increased by approximately 3.47% due to information loss.The proposed method was demonstrated to be rotationally invariant.The present study provided novel insights into evaluating mixing uniformity,which would guide enhanced heat transfer and the effective evaluation of the spatiotemporal characteristics of transient mixing in circular regions or the cross-sections of chemical transport pipelines. | Hui Sun Jianxin Xu Shibo Wang Guangcheng Cai Hua Wang Wuliang Yin | 2024 | Particuology2024，86，3： | 0 |
| 13 | Empirical Analysis of Forest Pest Control Efficiency from 2003 to 2014 in China显示文摘Three indexes including forest pest occurrence area,control area and input fund of 31 provinces from 2003 to 2014 were selected from Forestry Statistical Yearbook,to establish dynamic interaction index evaluation system with clustering robust regression model and Stata 13. 0 software. Total forest pest control efficiency in China was determined according to the computing result of entropy method. Suggestions such as improving forest pest control efficiency,increasing service efficiency and input amount of forest pest control input funds were put forward. It will provide empirical basis for target management evaluation of forest pest control work and accountability system. | Cai Qi Cai Yushi Sun Shibo Ding Huimin Ren jie Wen Yali | 2017 | Plant Diseases and Pests2017，8，5： | 0 |
| 14 | Analogous comparison unravels heightened antiviral defense and boosted viral infection upon immunosuppression in bat organoids显示文摘Horseshoe bats host numerous SARS-related coronaviruses without overt disease signs.Bat intestinal organoids,a unique model of bat intestinal epithelium,allow direct comparison with human intestinal organoids.We sought to unravel the cellular mechanism(s)underlying bat tolerance of coronaviruses by comparing the innate immunity in bat and human organoids.We optimized the culture medium,which enabled a consecutive passage of bat intestinal organoids for over one year.Basal expression levels of IFNs and IFN-stimulated genes were higher in bat organoids than in their human counterparts.Notably,bat organoids mounted a more rapid,robust and prolonged antiviral defense than human organoids upon Poly(I:C)stimulation.TLR3 and RLR might be the conserved pathways mediating antiviral response in bat and human intestinal organoids.The susceptibility of bat organoids to a bat coronavirus CoV-HKU4,but resistance to EV-71,an enterovirus of exclusive human origin,indicated that bat organoids adequately recapitulated the authentic susceptibility of bats to certain viruses.Importantly,TLR3/RLR inhibition in bat organoids significantly boosted viral growth in the early phase after SARS-CoV-2 or CoV-HKU4 infection.Collectively,the higher basal expression of antiviral genes,especially more rapid and robust induction of innate immune response,empowered bat cells to curtail virus propagation in the early phase of infection. | Xiaojuan Liu Cun Li Zhixin Wan Man Chun Chiu Jingjing Huang Yifei Yu Lin Zhu Jian-Piao Cai Lei Rong You-qiang Song Hin Chu Zongwei Cai Shibo Jiang Kwok-yung Yuen Jie Zhou | 2023 | Signal Transduction and Targeted Therapy2023，8，1： | 0 |
| 15 | Pneumatic webbed soft gripper for unstructured grasping显示文摘Grasping unstructured and fragile objects such as food and fruits is a great challenge for robots.Being naturally different from the traditional rigid robot,soft robotics provide highly promising choices with their intrinsic flexibility and compliance to objects.Inspired by duck foot and octopus tentacle,a pneumatic webbed soft gripper was proposed,which is consisted of four multi-chambered fingers and four webs.Due to its silicone body and soft web structure,the developed soft gripper can naturally adapt,grasp and hold delicate and unstructured objects.Compressed air inflated into the three chambers of the finger actuates the silicone body and performs inflection and extension.The silicone web follows the motion of four fingers,forming a semi-closed grasping configuration.The fingers were fabricated with silicone rubber and constraint spring by casting process.The web was cast around the fingers.The inflecting motion was modeled via the pneumatic principle and geometrical analysis.The dynamic properties of the finger were tested by step and sinusoidal signals.And the grasping performances for different objects,such as egg,strawberry,candy,and knife,were also demonstrated by experiments.The proposed soft gripper performed stably in response to a 0.4 Hz reference sinusoidal signal.The bionic structure greatly improves the stability and reliability of grasping,particularly for unstructured and fragile objects.Moreover,the webs ensure the grasping for multiple objects in one snatch,especially suitable for agricultural products and food processing. | Shibo Cai Chun’e Tang Lufeng Pan Guanjun Bao Wenyu Bai Qinghua Yang | 2021 | International Journal of Agricultural and Biological Engineering2021，14，4： | 0 |
| 16 | Gp350-anchored extracellular vesicles: promising vehicles for delivering therapeutic drugs of B cell malignancies显示文摘Although chimeric antigen receptor-modified(CAR)T cell therapy has been successfully applied in the treatment of acute B lymphocytic leukemia,its effect on Burkitt lymphoma(BL)and chronic B lymphocytic leukemia(B-CLL)is unsatisfactory.Moreover,fatal side effects greatly impede CAR T cell application.Extracellular vesicles(EVs)are excellent carriers of therapeutic agents.Nevertheless,EVs mainly accumulate in the liver when administered without modification.As an envelope glycoprotein of Epstein–Barr viruses,gp350 can efficiently bind CD21 on B cells.Here,gp350 was directly anchored onto red blood cell EVs(RBC-EVs)via its transmembrane region combined with low-voltage electroporation.The results showed that gp350 could anchor to RBC-EVs with high efficiency and that the resulting gp350-anchored RBC-EVs(RBC-EVs/gp350^(Etp))exhibited increased targeting to CD21+BL and B-CLL relative to RBC-EVs.After the loading of doxorubicin or fludarabine,RBC-EVs/gp350^(Etp) had powerful cytotoxicity and therapeutic efficacy on CD21+BL or B-CLL,respectively.Moreover,RBC-EVs/gp350^(Etp) loaded with a drug did not exhibit any apparent systemic toxicity and specifically induced the apoptosis of tumor B cells but not normal Bcells.Therefore,our findings indicate that drug-loaded RBC-EVs/gp350^(Etp) may be adopted in the treatment of CD21+B cell malignancies. | Huiqing Xiu Xi Nan Danfeng Guo JiaoliWang Jiahui Li Yanmei Peng Guirun Xiong Shibo Wang Changjun Wang Gensheng Zhang Yunshan Yang Zhijian Cai | 2022 | Asian Journal of Pharmaceutical Sciences2022，17，3： | 0 |
| 17 | Ultralong Stretchable Soft Actuator(US2A):Design,Modeling and Application显示文摘Actuator plays a significant role in soft robotics.This paper proposed an ultralong stretchable soft actuator(US2A)with a variable and sizeable maximum elongation.The US2A is composed of a silicone rubber tube and a bellows woven sleeve.The maximal extension can be conveniently regulated by just adjusting the wrinkles’initial angle of the bellows woven sleeve.The kinematics of US2A could be obtained by geometrically analyzing the structure of the bellows woven sleeve when the silicone rubber tube is inflated.Based on the principle of virtual work,the actuating models have been established:the pressure-elongation model and the pressure-force model.These models reflect the influence of the silicone tube’s shell thickness and material properties on the pneumatic muscle’s performance,which facilitates the optimal design of US2A for various working conditions.The experimental results showed that the maximum elongation of the US2A prototype is 257%,and the effective elongation could be variably regulated in the range of 0 and 257%.The proposed models were also verified by pressure-elongation and pressure-force experiments,with an average error of 5%and 2.5%,respectively.Finally,based on the US2A,we designed a pneumatic rehabilitation glove,soft arm robot,and rigid-soft coupling continuous robot,which further verified the feasibility of US2A as a soft driving component. | Wenbiao Wang Yunfei Zhu Shibo Cai Guanjun Bao | 2023 | Chinese Journal of Mechanical Engineering2023，36，1： | 0 |
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