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| 1 | Obtained transgenic wheat expressing pac1 mediated by Agrobacterium is resistant against Barley yellow dwarf virus-GPV显示文摘In fission yeast (Schizosaccharomyces pombe), pac1 gene was cloned with 99.3% nucleo- tide sequence similarity with published pac1 in GenBank. In pET-5α expression system, the expres- sion product of cloned pac1 in E. coli showed activity to degrade the double-strand RNA. Harboring the binary vector pBI121, which contains pac1 gene, Agrobacterium tumefaciens strain LBA4404 was used to transform the wheat immature embryos pre- cultured 7―10 d. After preregeneration, regeneration and selection culture stage, totally 41 G418 resistant plants were obtained, in which 25 lines were proved to integrate with transgene and express transgene normally by PCR, Dot blot, RT-PCR and ELISA de- tection. Antivirus test carried out on 25 positive lines with high dose of Barley yellow dwarf virus-GPV re- vealed that 12 lines had resistance to BVDV-GPV in low level, another 12 lines had resistance to BVDV- GPV in middle level, and 1 line showed resistance to BVDV-GPV in high level. However, both low and middle level of resistance plants showed no symp- toms when infected by viruses at low dose, which suggested the dose-dependent effect of the resis- tance mediated by pac1 to BYDV-GPV. | YAN Fei ZHENG Yinying ZHANG Wenwei XIAO Hong LI Shifang CHENG Zhuomin | 2006 | Chinese Science Bulletin2006，51，19： | 5 |
| 2 | Molecular dynamics simulation of polycrystalline molybdenum nanowires under uniaxial tensile strain : size effects 显示文摘 | Xiaofan Li Wangyu Hu Shifang Xiao Wei-Qing Huang | 2008 | Physica E2008，40，10： | 1 |
| 3 | Melting evolution and diffusion behavior of vanadium nanoparticles显示文摘 | Wangyu Hu Shifang Xiao Jianyu Yang Zhi Zhang | 2005 | The European Physical Journal B2005，，4： | 1 |
| 4 | 中国大鲵隐存种及其灭绝危机显示文摘文章简介由于栖息地破坏及人为过度捕捉,中国大鲵的野生资源锐减。由于缺乏显著的外部性状差异,中国大鲵长期以来被认为是一个物种。不同地方的种群是否存在显著的分化?目前基于'一个物种'的保护策略是否得当. | 颜芳 Jingcai Lü Baolin Zhang Zhiyong Yuan Haipeng Zhao Song Huang Gang Wei Xue Mi Dahu Zou Wei Xu Shu Chen Jie Wang Feng Xie Minyao Wu Hanbin Xiao Zhiqiang Liang Jieqiong Jin Shifang Wu CunShuan Xu Benjamin Tapley Samuel T.Turvey Theodore J.Papenfuss Andrew A.Cunningham Robert W.Murphy 张亚平 车静 | 2019 | 科学新闻2019，0，2： | 1 |
| 5 | Size effect on the thermodynamic properties of silver nanoparticles 显示文摘 | Luo Wenhua Hu Wangyu Xiao Shifang | 2008 | Journal of Physical Chemistry C2008，112，： | 1 |
| 6 | Toward green catalytic synthesis—Transition metal-catalyzed reactions in non-conventional media显示文摘 | Shifang Liu Jianliang Xiao | 2007 | Journal of Molecular Catalysis A Chemical2007，，1： | 1 |
| 7 | Shock waves propagation and phase transition in single crystal iron under ramp compression: large scale parallel NEMD simulations显示文摘 | Wang Kun Xiao Shifang Liu Meng | 2013 | Procedia Engineering2013，61，： | 1 |
| 8 | An atomic study on the shock-induced plasticity and phase transition for iron-based single crystals显示文摘 | Wang Kun Xiao Shifang Deng Huiqiu | 2014 | International Journal of Plasticity2014，59，： | 1 |
| 9 | Coupling between plasticity and phase transition of polycrystalline iron under shock compressions显示文摘 | Wang Kun Zhu Wenjun Xiao Shifang | 2015 | International Journal of Plasticity2015，，： | 1 |
| 10 | Population Ecology of Ilex cornuta in Longgan Lake National Nature Preserve,Hubei Province显示文摘The population size class structure,survival curve,height class structure and population distribution patterns of Ilex cornuta in Longgan Lake National Nature Preserve,Hubei Province,were investigated by using the adjacent grid method. The result showed that the population age structure of I. cornuta was of middle-aging type,in the vertical space of population individuals,most of them lived in the shrub layer,and a small number of individuals entered the arborous layer.The distribution pattern of I. cornuta was analyzed by variance/mean ratio method,and the results showed that the distribution pattern of the population was the cluster type. This study can provide references for the further protection,research and development and utilization of I. cornuta. | Junjie YANG Shifang SHAO Xiao GUO Yuanping FANG | 2018 | Agricultural Biotechnology2018，7，5： | 0 |
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