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| 1 | A semi-spring and semi-edge combined contact model in CDEM and its application to analysis of Jiweishan landslide显示文摘Continuum-based discrete element method(CDEM)is an explicit numerical method used for simulation of progressive failure of geological body.To improve the efficiency of contact detection and simplify the calculation steps for contact forces,semi-spring and semi-edge are introduced in calculation.Semispring is derived from block vertex,and formed by indenting the block vertex into each face(24semisprings for a hexahedral element).The formation process of semi-edge is the same as that of semi-spring(24semi-edges for a hexahedral element).Based on the semi-springs and semi-edges,a new type of combined contact model is presented.According to this model,six contact types could be reduced to two,i.e.the semi-spring target face contact and semi-edge target edge contact.By the combined model,the contact force could be calculated directly(the information of contact type is not necessary),and the failure judgment could be executed in a straightforward way(each semi-spring and semi-edge own their characteristic areas).The algorithm has been successfully programmed in C++program.Some simple numerical cases are presented to show the validity and accuracy of this model.Finally,the failure mode,sliding distance and critical friction angle of Jiweishan landslide are studied with the combined model. | Chun Feng Shihai Li Xiaoyu Liu Yanan Zhang | 2014 | Journal of Rock Mechanics and Geotechnical Engineering2014，6，1： | 34 |
| 2 | Novel metabolic and physiological functions of branched chain amino acids: a review显示文摘It is widely known that branched chain amino acids(BCAA) are not only elementary components for building muscle tissue but also participate in increasing protein synthesis in animals and humans. BCAA(isoleucine, leucine and valine) regulate many key signaling pathways, the most classic of which is the activation of the m TOR signaling pathway. This signaling pathway connects many diverse physiological and metabolic roles. Recent years have witnessed many striking developments in determining the novel functions of BCAA including:(1) Insufficient or excessive levels of BCAA in the diet enhances lipolysis.(2) BCAA, especially isoleucine, play a major role in enhancing glucose consumption and utilization by up-regulating intestinal and muscular glucose transporters.(3)Supplementation of leucine in the diet enhances meat quality in finishing pigs.(4) BCAA are beneficial for mammary health, milk quality and embryo growth.(5) BCAA enhance intestinal development, intestinal amino acid transportation and mucin production.(6) BCAA participate in up-regulating innate and adaptive immune responses.In addition, abnormally elevated BCAA levels in the blood(decreased BCAA catabolism) are a good biomarker for the early detection of obesity, diabetes and other metabolic diseases. This review will provide some insights into these novel metabolic and physiological functions of BCAA. | Shihai Zhang Xiangfang Zeng Man Ren Xiangbing Mao Shiyan Qiao | 2017 | Journal of Animal Science and Biotechnology2017，8，3： | 28 |
| 3 | BP-PSO-based intelligent case retrieval method for high-rise structural form selection显示文摘Modern architectures are developing in the direction of tall buildings and complex structures,and the theoretical analysis and the design experience have seriously lagged behind the construction of super high-rise structures.Structural form selection,especially the case based reasoning (CBR) based structural form selection,is a promising tool for the construction of high-rise structures.In view of the limit of cognitive ability of domain experts,a BP (back propagation)-PSO (particle swarm optimization)-based intelligence case retrieval method for high-rise structural form selection is proposed.The CBR-based case retrieval method and the construction of the BP-PSO neutral network are introduced.And then the BP-PSO-based case retrieval method is validated by some engineering cases.The results of training and prediction indicate that the proposed method has good ability to retrieve the cases of high-rise structures. | ZHANG ShiHai OU JinPing | 2013 | Science China(Technological Sciences)2013，56，4： | 9 |
| 4 | A fundamental theorem for eco-environmental surface modelling and its applications显示文摘We propose a fundamental theorem for eco-environmental surface modelling(FTEEM) in order to apply it into the fields of ecology and environmental science more easily after the fundamental theorem for Earth’s surface system modeling(FTESM). The Beijing-Tianjin-Hebei(BTH) region is taken as a case area to conduct empirical studies of algorithms for spatial upscaling, spatial downscaling, spatial interpolation, data fusion and model-data assimilation, which are based on high accuracy surface modelling(HASM), corresponding with corollaries of FTEEM. The case studies demonstrate how eco-environmental surface modelling is substantially improved when both extrinsic and intrinsic information are used along with an appropriate method of HASM. Compared with classic algorithms, the HASM-based algorithm for spatial upscaling reduced the root-meansquare error of the BTH elevation surface by 9 m. The HASM-based algorithm for spatial downscaling reduced the relative error of future scenarios of annual mean temperature by 16%. The HASM-based algorithm for spatial interpolation reduced the relative error of change trend of annual mean precipitation by 0.2%. The HASM-based algorithm for data fusion reduced the relative error of change trend of annual mean temperature by 70%. The HASM-based algorithm for model-data assimilation reduced the relative error of carbon stocks by 40%. We propose five theoretical challenges and three application problems of HASM that need to be addressed to improve FTEEM. | Tianxiang YUE Na ZHAO Yu LIU Yifu WANG Bin ZHANG Zhengping DU Zemeng FAN Wenjiao SHI Chuanfa CHEN Mingwei ZHAO Dunjiang SONG Shihai WANG Yinjun SONG Changqing YAN Qiquan LI Xiaofang SUN Lili ZHANG Yongzhong TIAN Wei WANG Ying’an WANG Shengnan MA Hongsheng HUANG Yimin LU Qing WANG Chenliang WANG Yuzhu WANG Ming LU Wei ZHOU Yi LIU Xiaozhe YIN Zong WANG Zhengyi BAO Miaomiao ZHAO Yapeng ZHAO Yimeng JIAO Ufra NASEER Bin FAN Saibo LI Yang YANG John PWILSON | 2020 | Science China Earth Sciences2020，63，8： | 7 |
| 5 | Effects of Nd^(3+)on the microstructure and magnetic properties of Ni-Zn ferrites显示文摘Ni0.4Zn0.6Fe2 ? 铁酸盐与 Nd2O3 的不同数量做了的 xNdxO4 (x = 0-0.07 ) 用标准陶器的技术被准备。样品单轴地被压并且在 1250D 的 | FAN Xiufeng REN Huiping ZHANG Yanghuan GUO Shihai WANG Xinlin | 2008 | Rare Metals2008，27，3： | 7 |
| 6 | Safety evaluation of Dagangshan arch dam resisting strong earthquakes with a rate-dependency anisotropic damage model显示文摘This paper presents a model to simulate the safe behavior of Dagangshan arch dam with a rate-dependency anisotropic damage model. This model considers the damage of asymmetry and anisotropy under cyclic loading of tension and compression, and it is used in the compiled finite element code. The material parameters used in the model can be identified from uniaxial static and dynamic experiments. Thereafter, it is used for analyzing damage and failure patterns of the dam subjected to water pressure and strong earthquakes. The numerical results show that it is necessary to consider both asymmetry between tension and compression and anisotropy of damage. Severe damage regions of the dam reveal brittle and risky positions clearly. Meanwhile damage patterns show the failure trend and safety behaviors of the dam. These results match well with that of the experiments carried out in DUT. The proposed model may be used to predict the damage patterns and potential failure modes of concrete structures like the dam. And the aseismic performance of the dam can be figured out. | DU RongQiang ZHANG Qing CHEN ShiHai LIN Gao | 2011 | Science China(Technological Sciences)2011，54，3： | 6 |
| 7 | Optical observations of LIGO source GW 170817 by the Antarctic Survey Telescopes at Dome A,Antarctica显示文摘The LIGO detection of gravitational waves(GW) from merging black holes in 2015 marked the beginning of a new era in observational astronomy. The detection of an electromagnetic signal from a GW source is the critical next step to explore in detail the physics involved. The Antarctic Survey Telescopes(AST3),located at Dome A, Antarctica, is uniquely situated for rapid response time-domain astronomy with its continuous night-time coverage during the austral winter. We report optical observations of the GW source(GW 170817) in the nearby galaxy NGC 4993 using AST3. The data show a rapidly fading transient at around 1 day after the GW trigger, with the i-band magnitude declining from 17:23 ± 0:13 magnitude to 17:72 ± 0:09 magnitude in ~1:8 h. The brightness and time evolution of the optical transient associated with GW 170817 are broadly consistent with the predictions of models involving merging binary neutron stars. We infer from our data that the merging process ejected about ~10^(-2) solar mass of radioactive material at a speed of up to 30% the speed of light. | lei hu xuefeng wu igor andreoni michael c.b.ashley jeff cooke xiangqun cui fujia du zigao dai bozhong gu yi hu haiping lu xiaoyan li zhengyang li ensi liang liangduan liu bin ma zhaohui shang tianrui sun n.b.suntzeff charling tao syed a.uddin lifan wang xiaofeng wang haikun wen di xiao jin xu ji yang shihai yang xiangyan yuan hongyan zhou hui zhang jilin zhou zonghong zhu | 2017 | Science Bulletin2017，62，21： | 6 |
| 8 | Phase transformation,thermodynamics and kinetics property of Mg90Ce5RE5(RE=La,Ce,Nd)hydrogen storage alloys显示文摘The Mg90Ce5 RE5(RE=La,Ce,Nd)alloys were prepared by a vacuum induction furnace and their micro structure,phase transformation,thermodynamics and kinetics property were systematically studied by XRD,SEM,TEM,and PCT characterization methods.The result shows that the activated alloys are composed of Mg/MgH2 and corresponding REH2+x with nanoscale.The REH2+x grain with Ce and La or Nd functional group have lower nucleation potential barriers than CeH2+x grains as the nucleation location,thus improve the hydrogen absorption kinetics of these alloys among which the Mg90Ce5Nd5 alloy can absorb 90%of the hydrogen within 2 min at 320℃.In addition,the Mg90Ce10 alloy has the lowest activation energy with 103.2 kJ mol-1 and the fastest desorption kinetics,which can release 5 wt%of the hydrogen within 20 min at 320℃.This is a correlation with grain size and the in-suit formed CeH2.73/CeO2 interface.Moreover,the co-doping Ce and La or Nd can effectively disorganize the thermodynamic stability of Mg-based hydrogen storage alloys to a certain degree,but the dehydrogenation kinetics of that still is restricted by the recombination energy of hydrogen ions on the surface. | Hui Yong Shihai Guo Zeming Yuan Yan Qi Dongliang Zhao Yanghuan Zhang | 2020 | Journal of Materials Science & Technology2020，47，16： | 5 |
| 9 | Deformation Measurement of Glass Structure Using FBG Sensor显示文摘Glass has been widely used as an important component in structures such as reflection glass curtainwalls,high speed trains,and landscape glass bridges with advantages of transparent and easy to clean,which are exposed to extreme weather conditions and external loads.Over time,these factors can lead to a damage of glass.So the health status of glass structure is critical,which should be routinely monitored to improve safety and provide reliable maintenance strategy.In this paper,fiber Bragg grating(FBG)sensors are used to monitor glass damage based on the fact that the main components of both the optical fiber and the glass are silica,which hints that both optical fiber and glass have the similar mechanical properties.Furthermore,the diameter of FBG installed on the glass structure is small,which has little effect on the beauty of glass.In order to validate the feasibility of the damage monitoring method,one common glass panel model with two-side fixations is loaded impact and static loads respectively,on the upper and lower surfaces of which four FBG sensors and two resistance strain gages are installed.A comparison study among the measured strains from the FBG sensors,those from the resistance strain gages,and those calculated from finite element model(FEM)analysis is conducted and the result obtained with experiments agrees with the element result.Test results show that the FBG sensors can effectively measure the glass deformation or damage under the impact and static load. | Bin LIU Shihai ZHANG Jianping HE | 2019 | Photonic Sensors2019，9，4： | 5 |
| 10 | Impacts of energy feeds and supplemental protease on growth performance, nutrient digestibility, and gut health of pigs from 18 to 45 kg body weight显示文摘A total of 144 pigs with 18.4 ± 2.3 kg initial body weight(BW) at 6 wk of age were used in a 40-d trial to evaluate effects of protease(300,000 U/kg feed, BioResource International Inc., Durham, NC, USA) on growth performance, apparent ileal digestibility(AID) of nutrients, and gut health of pigs fed diets with sorghum. Pigs were randomly allotted to 4 treatments(12 pens per treatment, 3 pigs per pen) in a 2 x 2 factorial arrangement(corn or sorghum basal diets, and 0 or 0.05% protease as 2 factors) with sex and initial BW as blocks. Experimental period had phase 1(d 1 to 21) and phase 2(d 22 to 40). About 65%(phase 1) and 72%(phase 2) of cereal grains were used in corn or sorghum based diets. Both grains were ground to 400 μm. Body weight and feed intake were recorded weekly. On d 35, serum was collected to quantify tumor necrosis factor-a(TNF-a) and malondialdehyde(MDA). Titanium dioxide(0.3%) was added as an indigestible marker for an additional 4 d feeding. On d 40, 32 pigs(8 pigs per treatment)were euthanized to collect digesta from jejunum and ileum(for viscosity and AID), tissues(for morphology) and mucosa samples(for TNF-α and MDA) from duodenum, jejunum, and ileum. Replacing corn with sorghum in the diet increased(P < 0.05) overall average daily gain(from 756 to 787 g/day) and average daily feed intake(from 1,374 to 1,473 g/day), reduced(P < 0.05) overall gain:feed ratio(from0.553 to 0.537), and did not affect AID. Pigs fed diets with sorghum had lower(P < 0.05) MDA content in serum(from 14.61 to 6.48 μmol/L) and jejunum(from 1.42 to 0.91 μmol/g protein), and reduced(P < 0.05) villus height(from 492 to 396 μm) and crypt depth(from 310 to 257 μm) in jejunum. Dietary protease improved(P < 0.05) AID of crude protein(from 81.8% to 86.0%), decreased MDA level(from 1.20 to 0.98 μmol/g protein) in duodenum, and increased(P < 0.05) the ratio of villus height to crypt depth(from 1.08 to 1.21) in duodenum. Overall, use of sorghum fully replacing corn in diets could benefit pigs with enhanced growth and feed intake potentially by reducing oxidative stress, whereas feed efficiency was compromised. Supplementation of protease improved protein digestion and maintained gut health,irrespective of sorghum or corn based diets. | Hongyu Chen Shihai Zhang Inkyung Park Sung Woo Kim | 2017 | Animal Nutrition2017，，4： | 4 |
| 11 | Nutritional strategies to alleviate oxidative stress in sows显示文摘The performance of high-yielding sows is directly related to the productivity of pig farming. Fetaldevelopment mainly occurs during the last month of pregnancy, and the aggressive metabolic burden ofsows during this stage eventually leads to systemic oxidative stress. When affected by oxidative stress,sows exhibit adverse symptoms such as reduced feed intake, hindered fetal development, and evenabortion. In addition, milk synthesis during the lactation period causes a severe metabolic burden. Thebiological response to oxidative stress during this period is associated with a decrease in milk production,which further affects the growth of piglets. Understanding the nutritional strategies to alleviateoxidative stress in sows is crucial to maintain their reproduction and lactation performance. Recently,advances have been made in the field of nutrition to relieve oxidative stress in sows during late pregnancyand lactation. This review highlights the nutritional strategies to relieve oxidative stress in sowsreported within the last 20 years. | Qihui Li Siwang Yang Fang Chen Wutai Guan Shihai Zhang | 2022 | Animal Nutrition2022，，2： | 4 |
| 12 | Martensitic Transformation and Magnetic-Field-Induced Strain in Magnetic Shape Memory Alloy NiMnGa Melt-Spun Ribbon显示文摘A magnetic shape memory alloy with nonstoichiometric Ni5oMn27Ga23 was prepared by using melt-spinning technology. The martensitic transformation and the magnetic-field-induced strain (MFIS) of the polycrystalline melt-spun ribbon were investigated. The experimental results showed that the melt-spun ribbons underwent thermal-elastic martensitic transformation and reverse transformation in cooling and heating process and exhibited typical thermoelastic shape memory effect. However the start temperature for martensitic transformation decreased from 286 K for as-cast alloy to 254 K for as-quenched ribbon and Curie temperature remains approximately constant. A particular internal stress induced by melt-spinning resulted in the formation of a texture structure in the ribbons, which made the ribbons obtain larger martensitic transformation strain and MFIS. The internal stress was released substantially after annealing, which resulted in a decrease of MFIS of the ribbons. | Shihai GUO, Yanghuan ZHANG, Jianliang LI, Baiyun QUAN, Yan QI and Xinlin WANG Department of Functional Material Research, Central Iron and Steel Research Institute, Beijing 100081, China | 2005 | Journal of Materials Science & Technology2005，21，2： | 3 |
| 13 | Effects of L-lysine·H2SO4 product on the intestinal morphology and liver pathology using broiler model显示文摘Background: Lysine is used widely in livestock production due to the shortage of feed protein resources.Llysine·H2SO4 contains L-lysine sulphate as well as fermentation co-products which contain other amino acids and phosphorus.However,there are few articles about L-lysine·H2SO4 product regarding intestinal morphology and liver pathology of broiler chickens.In this article,we focus on the absorption and metabolism of L-lysine·H2SO4 revealed in the variation of intestinal morphology and liver pathology to determine the tolerance of chicks for L-lysine·H2SO4.Methods: To evaluate the tolerance of broilers for L-lysine·H2SO4,240 one day old broilers were allocated randomly to one of five dietary treatments which included corn-soybean diets containing 0,1%,4%,7% or 10% L-lysine·H2SO4(L-lysine content = 55%).Results: Supplementation of 1% L-lysine·H2SO4 in the diet had no negative effects.However,4%,7% or 10% Llysine·H2SO4 supplementation produced negative responses on broiler performance,carcass characteristics,blood biochemistry,and particularly on intestinal morphology and liver pathology compared with broilers fed the control diet.Conclusion: Our results show that supplementation with 1% L-lysine·H2SO4 had no negative effects on performance,carcass characteristics,blood biochemistry,intestinal morphology and liver pathology in broilers,but supplementation with 4%,7% or 10% L-lysine·H2SO4 produced a negative response,particularly with respect to intestinal morphology and liver pathology. | Hongmin Jia Ting He Haitao Yu Xiangfang Zeng Shihai Zhang Wenfeng Ma Jie Zhang Shiyan Qiao Xi Ma | 2019 | Journal of Animal Science and Biotechnology2019，10，3： | 3 |
| 14 | Growth–climate response and drought reconstruction from tree-ring of Mongolian pine in Hulunbuir,Northeast China显示文摘Aims Drought affected by atmosphere–ocean cycle is a dominant factor influencing tree radial growth of sandy Mongolian pine(Pinus sylvestris var.mongolica)and regional vegetation dynamics in Hulunbuir,China.However,historical droughts and its correlations with tree radial growth and atmosphere–ocean cycle in this area have been little tested.Methods We developed tree-ring chronologies of Mongolian pine from Hulunbuir,Inner Mongolia,China and analyzed the correlations between tree-ring width index,the normalized difference vegetation index and Palmer drought severity index(PDSI),then developed a linear model to reconstruct the drought variability from 1829 to 2009.Long-term trends and its linkages with atmosphere–ocean cycle were performed by the power spectral,wavelet and teleconnection analysis.Important Findings The local moisture variations affected largely the regional vegetation dynamics and tree-ring growth of Mongolia pine in the forest–grassland transition.Using tree-ring width chronology of Mongolian pine,the reconstruction explains 49.2%of PDSI variance during their common data period(1951–2005).The reconstruction gives a broad-scale regional representation of PDSI in the Hulunbuir area,with drought occurrences in the 1850s,1900s,1920s,mid-1930s and at the turn of the 21st century.Comparisons with other treering drought reconstructions and historical records reveal some common drought periods and drying trends in recent decades at the northern margin zones of the East Asian summer monsoon(EASM).The drying trends in these zones occurred earlier than weakening of the EASM.A REDFIT spectral analysis shows significant peaks at 7.2,3.9,2.7–2.8,2.4 and 2.2 years with a 0.05 significance level,and 36.9,18.1 and 5.0 years with 0.1 significance level.Wavelet analysis also shows similar cycles.Drought variations in the study area significantly correlated with sea surface temperatures in the western tropical Pacific Ocean and middle and northern Indian Ocean,and the Pacific Decadal Oscillation and North Atlantic Oscillation.This suggests a possible linkage with the El Niño-Southern Oscillation,the EASM and the Westerlies. | Zhongjie Shi Lihong Xu Linshui Dong Jixi Gao Xiaohui Yang Shihai Lü Chaoyang Feng Aiyun Song Hao Guo Xiao Zhang | 2016 | Journal of Plant Ecology2016，9，1： | 3 |
| 15 | Blue LED-pumped intense short-wave infrared luminescence based on Cr^(3+)-Yb^(3+)-co-doped phosphors显示文摘The growing demand for spectroscopy applications in the areas of agriculture,retail and healthcare has led to extensive research on infrared light sources.The ability of phosphors to absorb blue light from commercial LED and convert the excitation energy into long-wavelength infrared luminescence is crucial for the design of cost-effective and high-performance phosphor-converted infrared LEDs.However,the lack of ideal blue-pumped short-wave infrared(SWIR)phosphors with an emission peak longer than 900 nm greatly limits the development of SWIR LEDs using light converter technology.Here we have developed a series of SWIR-emitting materials with high luminescence efficiency and excellent thermal stability by co-doping Cr^(3+)-Yb^(3+) ion pairs into Lu_(0.2)Sc_(0.8)BO_(3) host materials.Benefitting from strong light absorption of Cr^(3+) in the blue waveband and very efficient Cr^(3+)→Yb^(3+) energy transfer,the as-synthesized Lu_(0.2)Sc_(0.8)BO_(3):Cr^(3+),Yb^(3+) phosphor emits intense SWIR light in the 900-1200 nm from Yb^(3+) under excitation with blue light at~460 nm.The optimized phosphor presents an internal quantum yield of 73.6%and the SWIR luminescence intensity at 100℃can still keep 88.4%of the starting value at 25℃.SWIR LED prototype device based on Lu0.2Sc0.8BO3∶Cr^(3+)Yb^(3+) phosphor exhibits exceptional luminescence performance,delivering SWIR radiant power of 18.4 mW with 9.3%of blue-to-SWIR power conversion efficiency and 5.0%of electricity-to-SWIR light energy conversion efficiency at 120 mA driving current.Moreover,under the illumination of high-power SWIR LED,covert information identification and night vision lighting have been realized,demonstrating a very bright prospect for practical applications. | Yan Zhang Shihai Miao Yanjie Liang Chao Liang Dongxun Chen Xihui Shan Kangning Sun Xiao-Jun Wang | 2022 | Light(Science & Applications)2022，11，6： | 3 |
| 16 | Phase evolution,hydrogen storage thermodynamics and kinetics of ternary Mg90Ce5Sm5 alloy显示文摘Greatly stable thermodynamics and sluggish kinetics impede the practical application of Mg-based hydrogen storage alloys.The modifications of composition and structure are important strategies in turning these hydrogen storage properties.In this study,Mg-based Mg90Ce5 Sm5 ternary alloy fabricated by vacuum induction melting was investigated to explore the performance and the reaction mechanism as hydrogen storage material by X-ray diffraction(XRD),scanning electron microscope(SEM),transmission electron microscopy(TEM) and pressure-composition isotherms(PCI) measurements.The results indicate that the Mg-based Mg90Ce5 Sm5 ternary alloy consists of two solid solution phases,including the major phases(Ce,Sm)5 Mg41 and the minor phases(Ce,Sm)Mg12.After hydrogen absorption,these phases transform into the MgH2 and(Ce,Sm)H2.73 phase,while after hydrogen desorption,the MgH2 transforms into the Mg phase,but the(Ce,Sm)H2.73 phases are not changed.The alloy has a reversible hydrogen capacity of about 5.5 wt% H2 and exhibits well isothermal hydrogen absorption kinetics.Activation energy of 106 kJ/mol was obtained from the hydrogen desorption data between 573 and 633 K,which also exhibits the enhanced kinetics compared with the pure MgH2 sample,as a result of bimetallic synergy catalysis function of(Ce,Sm)H2.73 phases.The rate of hydrogen desorption is controlled by the release and recombination of H2 from the Mg surface.Furthermore,the changes of enthalpy and entropy of hydrogen absorption/desorption were calculated to be-80.0 kJ/mol H2,-137.5 J/K/mol H2 and 81.2 kJ/mol H2,139.2 J/K/mol H2,respectively. | Hui Yong Shihai Guo Zeming Yuan Wei Zhang Yan Qi Dongliang Zhao Yanghuan Zhang | 2020 | Journal of Rare Earths2020，38，6： | 3 |
| 17 | Quantifying 3D cell-matrix interactions during mitosis and the effect of anticancer drugs on the interactions显示文摘The mechanical force between cells and the extracellular microenvironment is crucial to many physiological processes such as cancer metastasis and stem cell differentiation. Mitosis plays an essential role in all these processes and thus an in-depth understanding of forces during mitosis gains insight into disease diagnosis and disease treatment. Here, we develop a traction force microscope method based on monolayer fluorescent beads for measuring the weak traction force (tens of Pa) of mitotic cells in three dimensions. We quantify traction forces of human ovarian granulosa (KGN) cells exerted on the extracellular matrix throughout the entire cell cycle in three dimensions. Our measurements reveal how forces vary during the cell cycle, especially during cell division. Furthermore, we study the effect of paclitaxel (PTX) and nocodazole (NDZ) on mitotic KGN cells through the measurement of traction forces. Our results show that mitotic cells with high concentrations of PTX exert a larger force than those with high concentrations of NDZ, which proved to be caused by changes in the structure and number of microtubules. These findings reveal the key functions of microtubule in generating traction forces during cell mitosis and explain how dividing cells regulate themselves in response to anti-mitosis drugs. This work provides a powerful tool for investigating cell-matrix interactions during mitosis and may offer a potential way to new therapies for cancer. | Yongman Liu Jianye Wang Yong Su Xiaohai Xu Hong Liu Kainan Mei Shihai Lan Shubo Zhang Xiaoping Wu Yunxia Cao Qingchuan Zhang Shangquan Wu | 2021 | Nano Research2021，14，11： | 3 |
| 18 | Experimental observation of coherent interaction between laser and erbium ions ensemble doped in fiber at sub 10 mK显示文摘Rare-earth ions doped in solid-state materials are considered promising candidates for quantum information applications,especially for photonic quantum memory.Among them,erbium ions doped in an optical fiber have attracted a lot of attentions due to their ability to provide efficient photon-atom interaction on a telecom-C band compatible transition.The coherent photon-atom interaction,which is crucial for quantum memory,has not yet been investigated for erbium ions doped in fiber at the temperature of sub 10 m K with the magnetic environment.In this paper,we experimentally observe optical nutation,which results from the coherent interaction between laser and erbium ions ensemble,in a piece of 9.5-m-long fiber with the erbium concentration of 200 ppm.We also extract the transition dipole moment from the results of optical nutation and further investigate its dependence on laser wavelength and magnetic field.A transition dipole moment of(3.424±0.019)×10^-32 C·m is obtained at the wavelength of 1537 nm and magnetic field of 0.2 T.Our results could pave the way for realizing solid-state quantum networks at telecom-C band. | Qi XI Shihai WEI Chenzhi YUAN Xueying ZHANG You WANG Haizhi SONG Guangwei DENG Bo JING Daniel OBLAK Qiang ZHOU | 2020 | Science China(Information Sciences)2020，63，8： | 3 |
| 19 | IL-9 blockade attenuates inflammation in a murine model of methicillin-resistant Staphylococcus aureus pneumonia显示文摘Methicllin-resistant Staphylococcus aureus(MRSA)is an important etiology of pneumonia.Interleukin(L)-9 is a T helper 9(Th9)cytokine and participates in the pathogenesis of infectious diseases.Here,we investigated the role of IL9 by using an MRSA pneumonia animal model.The BALB/c mice underwent nasal inhalation with an ST239 MRSA strain to establish the mouse model of MRSA pneumonia,and a subset of mice were intravenously injected with IL9 neutral-izing antibody or immunoglobulin (lg)G.At 3 and 8 days postinfection,the peripheral blood,bronchioalveolar lavage fluid(BALF),and lung tissues were collected.The frequencies of Th9 cells and the levels of cytokines in peripheral blood,BALE and lung tissues were determined by flow cytometry and enzyme-linked immunosorbent assay(ELISA),respectively.The colony counts of MRSA in BALF and lung tissue were detected.The lung pathological changes were examined using hematoxylin and eosin staining.Data from flow cytometry,qRTPCR,and ELISA showed that MRSA infected mice exhibited higher frequency of Th9 cells and higher IL9 mRNA and protein levels in the peripheral blood,BALF and lung tissues of mice.In contrast,the neutralization of IL9 abrogated MRSA inoculation-induced Th9 cell generation and IL9 production in BALF and lung tissues.Furthermore,bacterial counting and histological examination showed that the numbers of bacteria in BALF and lungs and the lung pathological scores induced by MRSA inoculation were attenuated by the neutralization of IL9.Moreover,cell counting and ELISA results demonstrated that IL9 neutralization diminished the MRSA inoculation-induced count of neutrophils and macrophages and levels of pro-inflammatory cytokines in BALF Collectively,IL9 neutralization attenuated inflammation of MRSA pneumonia by regulating Th9/L9 expression. | Weihua Xu Keyin Tian Xiaoshuang Li Shihai Zhang | 2020 | Acta Biochimica et Biophysica Sinica2020，52，2： | 3 |
| 20 | Recent progress of porcine milk components and mammary gland function显示文摘As the only nutritional source for newborn piglets,porcine colostrum and milk contain critical nutritional and immunological components including carbohydrates,lipids,and proteins(immunoglobulins).However,porcine milk composition is more complex than these three components.Recently,scientists identified additional and novel components of sow colostrum and milk,including exosomes,oligosaccharides,and bacteria,which possibly act as biological signals and modulate the intestinal environment and immune status in piglets and later in life.Evaluation of these nutritional and non-nutritional components in porcine milk will help better understand the nutritional and biological function of porcine colostrum and milk.Furthermore,some important functions of the porcine mammary gland have been reported in recent published literature.These preliminary studies hypothesized how glucose,amino acids,and fatty acids are transported from maternal blood to the porcine mammary gland for milk synthesis.Therefore,we summarized recent reports on sow milk composition and porcine mammary gland function in this review,with particular emphasis on macronutrient transfer and synthesis mechanisms,which might offer a possible approach for regulation of milk synthesis in the future. | Shihai Zhang Fang Chen Yinzhi Zhang Yantao Lv Jinghui Heng Tian Min Lilang Li Wutai Guan | 2019 | Journal of Animal Science and Biotechnology2019，10，1： | 2 |
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