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| 1 | Novel metabolic and physiological functions of branched chain amino acids: a review显示文摘It is widely known that branched chain amino acids(BCAA) are not only elementary components for building muscle tissue but also participate in increasing protein synthesis in animals and humans. BCAA(isoleucine, leucine and valine) regulate many key signaling pathways, the most classic of which is the activation of the m TOR signaling pathway. This signaling pathway connects many diverse physiological and metabolic roles. Recent years have witnessed many striking developments in determining the novel functions of BCAA including:(1) Insufficient or excessive levels of BCAA in the diet enhances lipolysis.(2) BCAA, especially isoleucine, play a major role in enhancing glucose consumption and utilization by up-regulating intestinal and muscular glucose transporters.(3)Supplementation of leucine in the diet enhances meat quality in finishing pigs.(4) BCAA are beneficial for mammary health, milk quality and embryo growth.(5) BCAA enhance intestinal development, intestinal amino acid transportation and mucin production.(6) BCAA participate in up-regulating innate and adaptive immune responses.In addition, abnormally elevated BCAA levels in the blood(decreased BCAA catabolism) are a good biomarker for the early detection of obesity, diabetes and other metabolic diseases. This review will provide some insights into these novel metabolic and physiological functions of BCAA. | Shihai Zhang Xiangfang Zeng Man Ren Xiangbing Mao Shiyan Qiao | 2017 | Journal of Animal Science and Biotechnology2017，8，3： | 28 |
| 2 | Size distribution of chemical elements and their source apportionment in ambient coarse, fine, and ultrafine particles in Shanghai urban summer atmosphere显示文摘Ambient coarse particles (diameter 1.8-10 μm), fine particles (diameter 0.1-1.8 μm), and ultrafine particles (diameter < 0.1 μm) in the atmosphere of the city of Shanghai were sampled during the summer of 2008 (from Aug 27 to Sep 08). Microscopic characterization of the particles was investigated by scanning electron microscopy coupled with energy dispersive X-ray spectroscopy (SEM/EDX). Mass concentrations of Si, P, S, Cl, K, Ca, Ti, V, Cr, Mn, Fe, Ni, Cu, Zn, As, Se, Br, Rb, Sr, and Pb in the size-resolved particles were quantified by using synchrotron radiation X-ray fluorescence (SRXRF). Source apportionment of the chemical elements was analyzed by means of an enrichment factor method. Our results showed that the average mass concentrations of coarse particles, fine particles and ultrafine particles in the summer air were 9.38 ± 2.18, 8.82 ± 3.52, and 2.02 ± 0.41 μg/m3, respectively. The mass percentage of the fine particles accounted for 51.47% in the total mass of PM10, indicating that fine particles are the major component in the Shanghai ambient particles. SEM/EDX results showed that the coarse particles were dominated by minerals, fine particles by soot aggregates and fly ashes, and ultrafine particles by soot particles and unidentified particles. SRXRF results demonstrated that crustal elements were mainly distributed in the coarse particles, while heavy metals were in higher proportions in the fine particles. Source apportionment revealed that Si, K, Ca, Fe, Mn, Rb, and Sr were from crustal sources, and S, Cl, Cu, Zn, As, Se, Br, and Pb from anthropogenic sources. Levels of P, V, Cr, and Ni in particles might be contributed from multi-sources, and need further investigation. | Senlin Lu Rui Zhang Zhenkun Yao Fei Yi Jingjing Ren Minghong Wu Man Feng Qingyue Wang | 2012 | Journal of Environmental Sciences2012，24，5： | 22 |
| 3 | The ultratrace detection of crystal violet using surface enhanced Raman scattering on colloidal Ag nanoparticles prepared by electrolysis显示文摘高度活跃、稳定、买得起的表面提高了散布的拉曼(重量的单位)底层被 electrolyzing 获得 AgNO 3的混合物( 4 × ; 10 −4 mol/L )并且 Na 3 C 6 H 5 O 7·H2 O ( 6 × ; 10 −5 mol/L )为 1 ,在 7 V 的 2 , 3 和 4 h 。与水晶紫(CV ) 作为一个测试分子,有 785 nm 激光刺激的一个便携式的拉曼分光计被采用执行 SERS 察觉。胶体的 Ag nanoparticles 与粒子尺寸为 3 h 由 electrolyzing 准备了(65 ± 17 ) nm 是为 CV 的极端踪迹察觉的完美的重量的单位底层,它显示了 ca 的一个改进因素。1.3 × 10 8 和 CV 的察觉限制对 ca 感到不满。10 −15 mol/L (ca。10 −4 ppb ) 与是的 10 −1 mol/L KBr 聚集代理人。因此,这重量的单位底层将在极端踪迹察觉提供有希望的前景。同时,从实验室带给拉曼分析在 situ,即时察觉和鉴定处理将提供可能性。 | Ren Ming Liu Yi Pu Kang Xing Fa Zi Ming Jun Feng Man Cheng Min Zhen Si | 2009 | Chinese Chemical Letters2009，20，6： | 10 |
| 4 | Induction of OTUD4 by viral infection promotes antiviral responses through deubiquitinating and stabilizing MAVS显示文摘The activity and stability of the adapter protein MAVS (also known as VISA, Cardif and IPS-1), which critically mediates cellular antiviral responses, are extensively regulated by ubiquitination. However, the process whereby MAVS is deubiquitinated is unclear. Here, we report that the ovarian tumor family deubiquitinase 4 (OTUD4) targets MAVS for deubiquitination. Viral infection leads to the IRF3/7-dependent upregulation of OTUD4 which interacts with MAVS to remove K48-linked polyubiquitin chains, thereby maintaining MAVS stability and promoting innate antiviral signaling. Knockout or knockdown of OTUD4 impairs RNA virus-triggered activation of IRF3 and NF-κB, expression of their downstream target genes, and potentiates VSV replication in vitro and in vivo. Consistently, Cre-ER Otud4fl/fl or Lyz2-Cre Otud4fl/fl mice produce decreased levels of type I interferons and proinflammatory cytokines and exhibit increased sensitivity to VSV infection compared to their control littermates. In addition, reconstitution of MAVS into OTUD4-deficient cells restores virus-induced expression of downstream genes and cellular antiviral responses. Together, our findings uncover an essential role of OTUD4 in virus-triggered signaling and contribute to the understanding of deubiquitination-mediated regulation of innate antiviral responses. | Tianzi Liuyu Keying Yu Liya Ye Zhidong Zhang Man Zhang Yujie Ren Zeng Cai Qiyun Zhu Dandan Lin Bo Zhong | 2019 | Cell Research2019，29，1： | 7 |
| 5 | MicroRNA-1304 suppresses human non-small cell lung cancer cell growth in vitro by targeting heme oxygenase-1显示文摘以前的研究证明了 microRNA-1304 (miR-1304 ) 是在癌症的某些类型的 dysregulated,包括非小的房间肺癌症(NSCLC ) ,并且可能涉及肿瘤幸存或生长。在这研究,我们在 vitro 在 NSCLC 调查了 miR-1304 和它的功能的直接目标。人的肺腺癌房间线(A549 和 NCI-H1975 ) 被学习。房间增长和幸存经由房间数, MTT 和殖民地形成试金被调查。房间 apoptosis 和房间周期用分别地染色试金的 annexin V-PE/7-AAD 和 PI 被检验。双酶的记者试金被用来由 miR-1304 验证 heme oxygenase-1 (HO-1 ) 的 post-transcriptional 规定。CRISPR/Cas9 被用来弄空内长的 miR-1304。MiR-1304 的 Overexpression 显著地减少了 NSCLC 房间和殖民地形成的数字和生存能力,和导致的房间 apoptosis 和 G 0/G1 阶段房间骑车拘捕。HO-1 被表明是在 NSCLC 房间的 miR-1304 的一个直接目标。由 hemin (20 mol/L ) 的 HO-1 表示的恢复在细胞生长上废除了 miR-1304 的抑制并且在 A549 细胞救了 miR-1304-induced apoptosis。有 anti-1304 的内长的 miR-1304 的抑制显著地增加了 HO-1 表示并且在 A549 房间支持了房间生长和幸存。在 17 件人的 NSCLC 织物样品, miR-1304 表示显著地被减少,当 HO-1 表示显著地作为与正常的肺纸巾相比被增加时。MicroRNA-1304 是肿瘤 suppressor, HO-1 是它在 NSCLC 的直接目标。结果为 NSCLC 作为一个治疗学的目标为 miR-1304 建议潜力。 | Cheng-gang LI Meng-fan PU Chun-zhu LI Man GAO Ming-xia LIU Cun-zhi YU Hong YAN Chun PENG Yang ZHAO Yu LI Ze-long MA Xin-ming QI Yi-zheng WANG Ling-ling MIAO Jin REN | 2017 | Acta Pharmacologica Sinica2017，38，1： | 6 |
| 6 | Optimization of Ultrasonic-Assisted Extraction Process of Polysaccharides from American Ginseng and Evaluation of Its Immunostimulating Activity显示文摘Ultrasonic-assisted extraction(UAE) of American ginseng polysaccharides(AGP) was investigated using response surface methodology. Three-factor-three-level Box-Behnken design was employed to optimize the ultrasonic power, extraction time and ratio of water to raw material to obtain a high AGP yield. The analysis of variance and response surface plots indicated that ultrasonic power was the most important factor affecting the extraction yield. The optimal conditions were ultrasonic power 400 W, extraction time 71 min, and ratio of water to raw material 33 m L g-1. Under these conditions, the yield of AGP was 8.09%, which was agreed closely to the predicted value. Gas chromatography(GC) analysis showed that AGP was composed of arabinose, rhamnose, galactose, glucose, and galacturonic acid. Fourier transform infrared spectra revealed the general characteristic absorption peaks of AGP. In addition, AGP exhibited good immunostimulating activities by up-regulating the production of nitric oxide and cytokines. Compared with hot water extraction, UAE required shorter extraction time and gave a higher extraction yield, without changing the structure and immunostimulating activity of AGP. The results indicated that UAE could be an effective and advisable technique for the large scale production of plant polysaccharides. | YANG Xiu-shi WANG Li-jun DONG Chuan Edmund Man King Lui REN Gui-xing | 2014 | Journal of Integrative Agriculture2014，13，12： | 5 |
| 7 | 4epatic cytochrome P450s play a major role in monocrotaline-induced renal toxicity in mice显示文摘 | Jun YAO Cheng-gang LI Li-kun GONG Chen-chen FENG Chun-zhu LI Man GAO Yang LUAN Xin-ming Qi Jin REN | 2014 | Acta Pharmacologica Sinica2014，35，2： | 4 |
| 8 | Effects of rosuvastatin on expression of angiotensin-converting enzyme 2 after vascular balloon injury in rats显示文摘Objective To investigate the effects and mechanisms of rosuvastatin on angiotensin -converting enzyme 2 (ACE2) in the process of neointimal formation after vascular balloon injury in rats, and to explore the effects of ACE2 and rosuvastatin in restenosis. Methods Thirty-six Wistar rats were randomly allocated into three groups: control group (n = 12), surgery group (n = 12), and statin group (n = 12). Aortic endothelial denudation of rats was performed using 2F balloon catheters. At days 14 and 28 after injury, aortic arteries were harvested to examine the following. Intimal thickening was examined by hematoxylin and eosin staining. We measured angiotensin II (Ang II) and angiotensin 1-7 (Ang-[1–7]) levels by a radioimmunological method or enzyme-linked immunosorbent assay. Protein andmRNAexpression of ACE2 and Ang II type 1 receptor (AT1) were investigated by immunohistochemistry, Western blots, and Reverse transcriptase- polymerase chain reaction (RT-PCR). We measured changes in proliferating cell nuclear antigen (PCNA) by immunohistochemistry. The level of phosphorylated extracellular signal regulated kinase 1/2 (P-ERK1/2) was evaluated byWestern blotting. Results Proliferation of vascular smooth muscle cells (VSMC) and intimal thickening were higher at day 14 after vascular balloon injury in the surgery group compared with the control group. Proliferation of VSMC was decreased by day 28 after injury, while intimal thickening continued. With rosuvastatin treatment, the extent of VSMC proliferation and intimal thickening was reduced at day 14 and 28 after injury. Ang II and P-ERK levels were significantly increased, Ang-(1–7) levels were significantly decreased, mRNA and protein expressions of ACE2 were significantly decreased, and AT1 expression was significantly increased at days 14 and 28 after vascular balloon injury in the surgery group compared with the control group. PCNAexpression was higher in the surgery group than in the control group, and it was significantly decreased after being given rosuvastatin. Expression of ACE2 mRNA and protein, and Ang-(1–7) levels were significantly increased, while AT1 expression and levels of Ang II and P-ERK were significantly decreased in the statin group compared with the surgery group. Conclusions Expression of ACE2 mRNA and protein is decreased in the process of intimal thickening after balloon injury. The inhibitory effect of rosuvastatin on intimal thickening is related to upregulation of ACE2, an increase in Ang-(1–7), downregulation of AT1, and activation of the P-ERK pathway. | Yong-Hong Li Qi-Xin Wang Jing-Wei Zhou Xian-Ming Chu Yu-Lin Man Ping Liu Bei-Bei Ren Ting-Ru Sun Yi An | 2013 | Journal of Geriatric Cardiology2013，10，2： | 3 |
| 9 | Plant multiscale networks:charting plant connectivity by multi-level analysis and imaging techniques显示文摘In multicellular and even single-celled organisms,individual components are interconnected at multiscale levels to produce enormously complex biological networks that help these systems maintain homeostasis for development and environmental adaptation.Systems biology studies initially adopted network analysis to explore how relationships between individual components give rise to complex biological processes.Network analysis has been applied to dissect the complex connectivity of mammalian brains across different scales in time and space in The Human Brain Project.In plant science,network analysis has similarly been applied to study the connectivity of plant components at the molecular,subcellular,cellular,organic,and organism levels.Analysis of these multiscale networks contributes to our understanding of how genotype determines phenotype.In this review,we summarized the theoretical framework of plant multiscale networks and introduced studies investigating plant networks by various experimental and computational modalities.We next discussed the currently available analytic methodologies and multi-level imaging techniques used to map multiscale networks in plants.Finally,we highlighted some of the technical challenges and key questions remaining to be addressed in this emerging field. | Xi Zhang Yi Man Xiaohong Zhuang Jinbo Shen Yi Zhang Yaning Cui Meng Yu Jingjing Xing Guangchao Wang Na Lian Zijian Hu Lingyu Ma Weiwei Shen Shunyao Yang Huimin Xu Jiahui Bian Yanping Jing Xiaojuan Li Ruili Li Tonglin Mao Yuling Jiao Sodmergen Haiyun Ren Jinxing Lin | 2021 | Science China(Life Sciences)2021，64，9： | 3 |
| 10 | Oblongifolin C suppresses lysosomal function independently of TFEB nuclear translocation显示文摘Lysosomes are the terminal organelles of the autophagic-endocytic pathway and play a key role in the degradation of autophagic contents. We previously reported that a natural compound oblongifolin C (OC) increased the number of autophagosomes and impaired the degradation of P62, most likely via suppression of lysosomal function and blockage of autophagosome-lysosome fusion. However, the precise mechanisms of how OC inhibits the lysosome-autophagy pathway remain unclear. In the present study, we investigated the effect of OC on transcription factor EB (TFEB), a master regulator of lysosomal biogenesis, lysosomal function and autophagy. We showed that treatment with OC (15 μM) markedly enhanced the nuclear translocation of TFEB in HeLa cells, concomitantly reduced the interaction of TFEB with 14-3-3 proteins. We further demonstrated that OC caused signi?cant inhibition of mTORC1 along with TFEB nuclear translocation, and OC-mediated TFEB nuclear translocation was dependent on mTORC1 suppression. Intriguingly, this increased nuclear TFEB was accompanied by reduced TFEB luciferase activity, increased lysosomal pH and impaired cathepsin enzyme activities. In HeLa cells, treatment with OC (7.5 μM) resulted in about 30% of cell death, whereas treatment with hydroxycitrate, a caloric restriction mimetic (20 μM) did not affect the cell viability. However, cotreatment with OC and hydroxycitrate caused signi?cantly great cytotoxicity (>50%). Taken together, these results demonstrate that inhibition of lysosome function is mediated by OC, despite evident TFEB nuclear translocation. | Man Wu Yuan-zhi Lao Hong-sheng Tan Guang Lu Yi Ren Zhao-qing Zheng Juan Yi Wen-wei Fu Han-ming Shen Hong-xi Xu | 2019 | Acta Pharmacologica Sinica2019，40，7： | 3 |
| 11 | Leucine modulates the IPEC-J2 cell proteome associated with cell proliferation, metabolism and phagocytosis显示文摘Leucine can affect intestinal protein expressions, and improve mucosal immune function. However, little study has been conducted to determine the change of protein component by leucine treatment in intestine epithelial cells. The present study was to cover the key proteins and cell pathways that could be regulated by leucine treatment in porcine intestinal epithelial cell line(IPEC-J2) cells with the approach of proteome analysis. A total number of 3,211 proteins were identified in our approach by searching the database of Uniprot sus scrofa. Among identified proteins, there were 101 proteins expressed differently between control group and leucine group. Compared with the control group, there were 50 up-regulated proteins and 51 down-regulated proteins in leucine group. In these proteins, leucine treatment decreased the expression of some proteins including pyruvate kinase, glyceraldehyde-3-phosphate dehydrogenase,E3 ubiquitin ligase, cathepsin D, caspase 3 and caspase 6, and increased the levels of some proteins, such as some eukaryotic translation initiation factors, ubiquitin carboxyl-terminal hydrolase, DNA-related RNA polymerase II, urokinase plasminogen activator, cyclin-dependent kinase inhibitor 2 b, Mut L homolog 1,5-methylcytosine binding domain 4, polymerase d, a-tubulin, syntaxin 18, Ras homolog D, actin related protein 2/3 complex and cofilin. Via the analysis of Gene Ontology and pathways, these proteins in IPECJ2 cells were related with some physiological functions, such as protein metabolism, glycolysis, cell proliferation, apoptosis and phagocytosis. Thus, these results suggest that leucine affects gut barrier function possibly via regulating cell proliferation and apoptosis, metabolism and phagocytosis. | Xiangbing Mao Man Ren Daiwen Chen Bing Yu Lianqiang Che Jun He Junqiu Luo Yuheng Luo Jianping Wang Hui Sun | 2018 | Animal Nutrition2018，，3： | 2 |
| 12 | Cathelicidins in inflammation and tissue repair: Potential therapeutic applications for gastrointestinal disorders显示文摘Cathelicidins,主人防卫肽的一个家庭,高度在感染,发炎并且创伤愈合期间被表示。这些肽不仅举办用途广泛的抗菌剂活动,而且由在 diseased 纸巾改变 cytokine 反应和煽动性的房间的 chemoattraction 调制发炎。在这个连接,老鼠 cathelicidin 被表明了通过提高粘液生产并且减少支持 inflammatory cytokines 的生产在冒号阻止发炎。另外, cathelicidins 支持创伤在受伤纸巾通过 re-epithelialization 和 angiogenesis 的刺激愈合。在胃的溃疡的一个动物模型,老鼠 cathelicidin 支持溃疡由在 vitro 并且在 vivo 导致胃的上皮的房间的增长愈合。在结论, cathelicidins 在在胃肠的 mucosa 和另外的机关操作发炎和组织修理的复杂集成的天生的免疫系统代表受动器分子的一个重要的组。 | William Ka Kei WU Clover Ching Man WONG Zhi Jie LI Lin ZHANG Shun Xiang REN Chi Hin CHO | 2010 | Acta Pharmacologica Sinica2010，31，9： | 2 |
| 13 | Prevalence of Covert Duplicate Publications in Budd-Chiari Syndrome Articles in China: A Systematic Analysis显示文摘 | Xingshun Qi Weirong Ren Lei Liu Zhiping Yang Man Yang Daiming Fan Guohong Han | 2013 | The American Journal of Medicine2013，，7： | 2 |
| 14 | A method for improving dispersion of starch nanocrystals in water through crosslinking modification with sodium hexametaphosphate显示文摘 | Ren Li li Jiang Man Wang Li yan | 2012 | Carbohydrate Polymer2012，87，2： | 1 |
| 15 | Delayed-Enhancement Cardiovascular Magnetic Resonance Coronary Artery Wall Imaging显示文摘 | Susan B. Yeon Adeel Sabir Melvin Clouse Pedro O. Martinezclark Dana C. Peters Thomas H. Hauser C. Michael Gibson Reza Nezafat David Maintz Warren J. Manning René M. Botnar | 2007 | Journal of the American College of Cardiology2007，，5： | 1 |
| 16 | Subclinical Coronary and Aortic Atherosclerosis Detected by Magnetic Resonance Imaging in Type 1 Diabetes With and Without Diabetic Nephropathy显示文摘 | Won Yong Kim Anne Sofie Astrup Matthias Stuber Lise Tarnow Erling Falk René M. Botnar Cheryl Simonsen Lotte Pietraszek Peter R. Hansen Warren J. Manning Niels T. Andersen Hans-Henrik Parving | 2007 | Circulation2007，，2： | 1 |
| 17 | Effect of inoculating white-rot fungus during different phases on the compost maturity of agricultural wastes显示文摘 | Guang Ming Zeng Hong Li Huang Dan Lian Huang Xing Zhong Yuan Rong Qing Jiang Man Yu Hong Yan Yu Jia Chao Zhang Ren You Wang Xiao Lan Liu | 2008 | Process Biochemistry2008，，4： | 1 |
| 18 | A method for improving dispersion of starch nanocrystals in water through crosslinking modification with sodium hexametaphosphate显示文摘 | Lili Ren Man Jiang Liyan Wang Jiang Zhou Jin Tong | 2011 | Carbohydrate Polymers2011，，2： | 1 |
| 19 | In Vivo Magnetic Resonance Imaging of Coronary Thrombosis Using a Fibrin-Binding Molecular Magnetic Resonance Contrast Agent显示文摘 | René M. Botnar Arno Buecker Andrea J. Wiethoff Edward C. Parsons Marcus Katoh George Katsimaglis Robert M. Weisskoff Randall B. Lauffer Philip B. Graham Rolf W. Gunther Warren J. Manning Elmar Spuentrup | 2004 | Circulation2004，，11： | 1 |
| 20 | Identification of a small molecule 1,4-bis-[4-(3- phenoxy-propoxy)-but-2-ynyl]-piperazine as a novel inhibitor of the transcription factor p53显示文摘 | Xin LIU Ying ZHANG Man TONG Xiu-ying LIU Guan-zheng LUO Dong-fang XIE Shao-fang REN Dong-hui BAI Liu WANG Qi ZHOU Xiu-jie WANG | 2013 | Acta Pharmacologica Sinica2013，34，6： | 1 |
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