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| 1 | Microscopic inspection and tracking of single upconversion nanoparticles in living cells显示文摘Nanoparticles have become new tools for cell biology imaging1,sub-cellular sensing2,super-resolution imaging3,4 and drug delivery5.Long-term 3D tracking of nanoparticles and their intracellular motions have advanced the understanding of endocytosis and exocytosis as well as of active transport processes6–8.The sophisticated operation of correlative optical-electron microscopy9,10 and scientific-grade cameras is often used to study intercellular processes.Nonetheless,most of these studies are still limited by the insufficient sensitivity for separating a single nanoparticle from a cluster of nanoparticles or their aggregates8,11,12.Here we report that our eyes can track a single fluorescent nanoparticle that emits over 4000 photons per 100 milliseconds under a simple microscope setup.By tracking a single nanoparticle with high temporal,spectral and spatial resolution,we show the measurement of the local viscosity of the intracellular environment.Moreover,beyond the colour domain and 3D position,we introduce excitation power density as the fifth dimension for our eyes to simultaneously discriminate multiple sets of single nanoparticles. | Fan Wang Shihui Wen Hao He Baoming Wang Zhiguang Zhou Olga Shimoni Dayong Jin | 2018 | Light(Science & Applications)2018，7，1： | 9 |
| 2 | Effects of acid,acid-ZVI/PMS,Fe(Ⅱ)/PMS and ZVI/PMS conditioning on the wastewater activated sludge(WAS)dewaterability and extracellular polymeric substances(EPS)显示文摘The effects of four conditioning approaches:Acid,Acid-zero-valent iron(ZVI)/peroxydisulfate(PMS),Fe(Ⅱ)/PMS and ZVI/PMS,on wastewater activated sludge(WAS)dewatering and organics distribution in supernatant and extracellular polymeric substances(EPS)layers were investigated.The highest reduction in bound water and the most WAS destruction was achieved by Acid-ZVI/PMS,and the optimum conditions were pH 3,ZVI dosage 0.15 g/g dry solid(DS),oxone dosage 0.07 g/g DS and reaction time 10.6 min with the reductions in capillary suction time(CST)and water content(Wc)as 19.67%and 8.49%,respectively.Four conditioning approaches could result in TOC increase in EPS layers and supernatant,and protein(PN)content in tightly bound EPS(TB-EPS).After conditioning,organics in EPS layers could migrate to supernatant.Polysaccharide(PS)was easier to migrate to supernatant than PN.In addition,Acid,Acid-ZVI/PMS or Fe(Ⅱ)/PMS conditioning promoted the release of some polysaccharides containing ring vibrations v P=O,v C-O-C,v C-O-P functional groups from TB-EPS.ESR spectra proved that both radicals of SO4-·and·OH contributed to dewatering and organics transformation and migration.CST value of WAS positively correlated with the ratios of PN/PS in LB-EPS and total EPS,while it negatively correlated with TOC,PN content and PS content in TB-EPS,as well as PS content in supernatant and LB-EPS.BWC negatively correlated to zeta potential and TOC value,PN content,and HA content in supernatant. | Xiaoyang Fan Yili Wang Daxin Zhang Yajie Guo Shihui Gao Enrui Li Huaili Zheng | 2020 | Journal of Environmental Sciences2020，32，5： | 4 |
| 3 | Asymmetric neurocognitive representation of ethnic in-group/out-group faces显示文摘To investigate asymmetric neurocognitive representation of ethnic in-group and out-group members, we recorded event-related potentials (ERPs) to faces in a perceptual task after the faces had been primed with positive or negative affective links. The affective link priming did not influence the ERPs to ethnic out-group faces. However, relative to the positive affective link priming, the negative affective link priming increased the amplitudes of an early frontal negativity (N100) and a following central negativity but decreased the amplitude of a late positive potential elicited by ethnic in-group faces. Moreover, the N100 amplitude correlated with the degree of negative attitudes towards ethnic in-group faces. The findings suggest that multiple-level neural mechanisms are involved in individuation of heterogeneous ethnic in-group faces. | MA YiNa GE JianQiao XU XiaoJing FAN Yan YANG ShengMin HAN ShiHui | 2009 | Chinese Science Bulletin2009，54，12： | 3 |
| 4 | Neural basis of cultural influence on self-representation显示文摘 | Ying Zhu Li Zhang Jin Fan Shihui Han | 2006 | Neuroimage2006，，3： | 1 |
| 5 | Temporal dynamic of neural mechanisms involved in empathy for pain: An event-related brain potential study显示文摘 | Yan Fan a b Shihui Hana b | 2008 | Neuropsychologia2008，，46： | 1 |
| 6 | Neural basis of cultural influence on self-representation显示文摘 | Ying Zhu Li Zhang Jin Fan Shihui Han | 2006 | Neuroimage2006，，3： | 1 |
| 7 | Neural activities underlying environmentaland personal risk identification tasks显示文摘 | Qin Jungang Lee Tatia M C Wang Fan Mao Lihua Han Shihui | 2009 | NeuroscienceLetters2009，455，2： | 1 |
| 8 | Biophysical and functional characterizations of recombinant RimI acetyltransferase from Mycobacterium tuberculosis显示文摘Nα-acetylation is a universal protein modification related to a wide range of physiological processes in eukaryotes and prokaryotes. RimI, an Nα-acetyltransferase in Mycobacterium tuberculosis, is responsible for the acetylation of the α-amino group of the N-terminal residue in the ribosomal protein S18. Despite growing evidence that protein acetylation may be correlated with the pathogenesis of tuberculosis, no structural information is yet available for mechanistically understanding the MtRimI acetylation. To enable structural studies for MtRimI, we constructed a serial of recombinant MtRimI proteins and assessed their biochemical properties. We then chose an optimal construct MtRimIC21A4-153 and expressed and purified the truncated high-quality protein for further biophysical and functional characterizations. The 2D 1H-15N heteronuclear single quantum coherence spectrum of MtRimIC21A4-153 exhibits wider chemical shift dispersion and favorable peak isolation, indicating that MtRimIC21A4-153 is amendable for further structural determination. Moreover, bio-layer interferometry experiments showed that MtRimIC21A4-153 possessed similar micromolar affinity to full-length MtRimI for binding the hexapeptide substrate Ala-Arg-Tyr-Phe-Arg-Arg. Enzyme kinetic assays also exhibited that MtRimIC21A4-153 had almost identical enzymatic activity to MtRimI, indicating insignificant influence of the recombinant variations on enzymatic functions. Furthermore, binding sites of the peptide were predicted by molecular docking approach, suggesting that this substrate binds to MtRimI primarily through electrostatic and hydrogen bonding interactions. Our results lay a foundation for the further structural determination and dynamics detection of MtRimI. | Meijing Hou Jie Zhuang Shihui Fan Huilin Wang Chenyun Guo Hongwei Yao Donghai Lin Xinli Liao | 2019 | Acta Biochimica et Biophysica Sinica2019，51，9： | 0 |
| 9 | Corrigendum to ‘Effects of acid, acid-ZVI/PMS, Fe(Ⅱ)/PMS and ZVI/PMS conditioning on the wastewater activated sludge (WAS) dewaterability and extracellular polymeric substances (EPS)' [J. Environ. Sci. 91 (2020) 73-84]显示文摘The authors regret'In the acknowledgments of this manuscript,the Nos.51678035 and 51478041 of the National NaturalScience Foundation of China are wrong.The correct Nos.are 51678053 and 51478041'.The authors would like to apologize for any inconvenience caused. | Xiaoyang Fan Yili Wang Daxin Zhang Yajie Guo Shihui Gao Enrui Li Huaili Zheng | 2021 | Journal of Environmental Sciences2021，33，1： | 0 |
| 10 | Crystal structure of the polyketide cyclase from Mycobacterium tuberculosis显示文摘About 40%of proteins are classified as conserved hypothetical proteins in Mycobacterium tuberculosis(TB).Identification and characterization of these proteins are beneficial to understand the pathogenesis of TB and exploiting novel drugs for TB treatments.The polyketide cyclase,a protein from M.tuberculosis(MtPC)has been annotated as a hypothetical protein in Uniprot database.Sequence analysis shows that the MtPC belongs to the NTF2-like superfamily proteins with diverse functions.Here,we determined the crystal structure of MtPC at a resolution of 2.4Åand measured backbone relaxation parameters for the MtPC protein.MtPC exists as a dimer in solution,and each subunit contains a six-stranded mixedβ-sheet and threeαhelixes which are arranged in the orderα1-α2-β1-β2-α3-β3-β4-β5-β6.The NMR dynamics analysis showed that the overall structure of MtPC is highly rigid on ps-ns time scales.Furthermore,we predicted the potential function of MtPC based on the crystal structure.Our results lay the basis for further exploiting and mechanistically understanding the biological functions of MtPC. | Jie Zhuang Shihui Fan Chenyun Guo Liubin Feng Huilin Wang Donghai Lin Xinli Liao | 2022 | Acta Biochimica et Biophysica Sinica2022，54，4： | 0 |
| 11 | Advances in regenerative medicine applications of tetrahedral framework nucleic acid-based nanomaterials: an expert consensus recommendation显示文摘With the emergence of DNA nanotechnology in the 1980s, self-assembled DNA nanostructures have attracted considerable attention worldwide due to their inherent biocompatibility, unsurpassed programmability, and versatile functions. Especially promising nanostructures are tetrahedral framework nucleic acids(t FNAs), first proposed by Turberfield with the use of a one-step annealing approach. Benefiting from their various merits, such as simple synthesis, high reproducibility, structural stability, cellular internalization, tissue permeability, and editable functionality, t FNAs have been widely applied in the biomedical field as threedimensional DNA nanomaterials. Surprisingly, t FNAs exhibit positive effects on cellular biological behaviors and tissue regeneration,which may be used to treat inflammatory and degenerative diseases. According to their intended application and carrying capacity,t FNAs could carry functional nucleic acids or therapeutic molecules through extended sequences, sticky-end hybridization,intercalation, and encapsulation based on the Watson and Crick principle. Additionally, dynamic t FNAs also have potential applications in controlled and targeted therapies. This review summarized the latest progress in pure/modified/dynamic t FNAs and demonstrated their regenerative medicine applications. These applications include promoting the regeneration of the bone,cartilage, nerve, skin, vasculature, or muscle and treating diseases such as bone defects, neurological disorders, joint-related inflammatory diseases, periodontitis, and immune diseases. | Yunfeng Lin Qian Li Lihua Wang Quanyi Guo Shuyun Liu Shihui Zhu Yu Sun Yujiang Fan Yong Sun Haihang Li Xudong Tian Delun Luo Sirong Shi | 2022 | International Journal of Oral Science2022，14，4： | 0 |
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