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| 1 | Improvement of wheat drought and salt tolerance by expression of a stress- inducible transcription factorGmDREB of soybean (Glycinemax)显示文摘Under stress conditions such as droughthigh-salinity and low-temperature, the transcription factorof DREB (dehydration responsive element binding proteins)improved efficiently stress resistance by regulating the ex-pression of its downstream genes with various environmentastress resistance in plants. GmDREB gene (GenBank Acces-sion No. AF514908) encoding a stress-inducible transcriptionfactor was cloned by screening a cDNA library of Glycinemax cv. Jinong 27 with yeast one-hybrid method. GmDREBgene was 910 bp in length and encoded 174 amino acids con-taining a conserved AP2/EREBP DNA-binding domain of 58amino acids. Two conserved functional amino acids, valineand glutamic acid, were located on the 14th and the 19thamino acid residues in the conserved structural domain. Analkaline amino acid region (KKR) related to a nuclear local-ization signal was at the N-terminal, while an acidic aminoacid region (DDD) related to trans-activation was at theC-terminal. Plant expression vectors were constructed andtransformed into wheat by bombardment. In total, 13 trans-genic plants with Ubi::GmDREB and 11 transgenic plantswith rd29A::GmDREB were identified from 103 regenerationplants by molecular analysis. The drought and salt tolerancesof T1 transgenic lines with Ubi::GmDREB orrd29A::GmDREB were demonstrated to be improved ascompared to wild type. The result also suggested that bothUbiquitin and rd29A promoters could effectively drive theexpression of the GmDREB gene and enhance drought andsalt tolerance of T1 plants. | GAO Shiqing XU Huijun CHENG Xianguo CHEN Ming XU Zhaoshi LI Liancheng YE Xingguo DU Lipu HAO Xiaoyan MA Youzhi | 2005 | Chinese Science Bulletin2005，50，23： | 30 |
| 2 | Doping bioactive elements into a collagen scaffold based on synchronous self-assembly/mineralization for bone tissue engineering显示文摘Pure collagen is biocompatible but lacks inherent osteoinductive,osteoimmunomodulatory and antibacterial activities.To obtain collagen with these characteristics,we developed a novel methodology of doping bioactive elements into collagen through the synchronous self-assembly/mineralization(SSM)of collagen.In the SSM model,amorphous mineral nanoparticles(AMN)(amorphous SrCO3,amorphous Ag3PO4,etc.)stabilized by the polyampholyte,carboxymethyl chitosan(CMC),and collagen molecules were the primary components under acidic conditions.As the pH gradually increased,intrafibrillar mineralization occurred via the self-adaptive interaction between the AMNs and the collagen microfibrils,which were self-assembling;the AMNs wrapped around the microfibrils became situated in the gap zones of collagen and finally transformed into crystals.Srdoped collagen scaffolds(Sr-CS)promoted in vitro cell proliferation and osteogenic differentiation of rat bone marrow mesenchymal stromal cells(rBMSCs)and synergistically improved osteogenesis of rBMSCs by altering the macrophage response.Ag-doped collagen scaffolds(Ag-CS)exhibited in vitro antibacterial effects on S.aureus,as well as cell/tissue compatibility.Moreover,Sr-CS implanted into the calvarial defect of a rat resulted in improved bone regeneration.Therefore,the SSM model is a de novo synthetic strategy for doping bioactive elements into collagen,and can be used to fabricate multifunctional collagen scaffolds to meet the clinical challenges of encouraging osteogenesis,boosting the immune response and fighting severe infection in bone defects. | Huanhuan Liu Mingli Lin Xue Liu Ye Zhang Yuyu Luo Yanyun Pang Haitao Chen Dongwang Zhu Xue Zhong Shiqing Ma Yanhong Zhao Qiang Yang Xu Zhang | 2020 | Bioactive Materials2020，5，4： | 6 |
| 3 | Sciatic nerve regeneration using a nerve growth factor-containing fibrin glue membrane显示文摘Our previous findings confirmed that the nerve growth factor-containing fibrin glue membrane provides a good microenvironment for peripheral nerve regeneration;however,the precise mechanism remains unclear.p75 neurotrophin receptor(p75NTR)plays an important role in the regulation of peripheral nerve regeneration.We hypothesized that a nerve growth factor-containing fibrin glue membrane can promote neural regeneration by up-regulating p75NTRexpression.In this study,we used a silicon nerve conduit to bridge a 15 mm-long sciatic nerve defect and injected a mixture of nerve growth factor and fibrin glue at the anastomotic site of the nerve conduit and the sciatic nerve.Through RT-PCR and western blot analysis,nerve growth factor-containing fibrin glue membrane significantly increased p75NTRmRNA and protein expression in the Schwann cells at the anastomotic site,in particular at 8 weeks after injection of the nerve growth factor/fibrin glue mixture.These results indicate that nerve growth factor-containing fibrin glue membrane can promote peripheral nerve regeneration by up-regulating p75NTRexpression in Schwann cells. | Shengzhong Ma Changliang Peng Shiqing Wu Dongjin Wu Chunzheng Gao | 2013 | Neural Regeneration Research2013，8，36： | 4 |
| 4 | Characterization of DNA damage response deficiency in pancreatic cancer patients from China显示文摘To the editor,Pancreatic ductal adenocarcinoma(PDAC)has the worst prognosis among all common malignant solid tumors,with a 5-year overall survival(OS)rate of less than 10%[1].Few effective targets for anticancer ther-apy have been confirmed in pancreatic cancer.Recently,it was substantiated that pancreatic cancer patients carry-ing deleterious mutations of the DNA damage response(DDR)genes are more likely to benefit from platinum-based chemotherapy[2]and poly(adenosine diphosphate-ribose)polymerase(PARP)inhibitor[3]. | Xiaofei Zhang Tiebo Mao Bei Zhang Haiyan Xu Jiujie Cui Feng Jiao Dongqin Chen Yu Wang Jiong Hu Qing Xia Shumin Li Ming Yue Jingyu Ma Jiayu Yao Yongchao Wang Xiao Zhang Shiqing Chen Yuezong Bai Yuexiang Wang Xuebin Zhang Qiang Liu Yongwei Sun Deliang Fu Yingbin Liu Lei Xiong Liwei Wang | 2022 | Cancer Communications2022，42，1： | 2 |
| 5 | Rapid and effective treatment of traumatic spinal cord injury using stem cell derived exosomes显示文摘Traumatic spinal cord injury is a fatal acute event without effective clinical therapies.Following the trauma,immediate neural protection and microenvironment mitigation are vitally important for nerve tissue repair,where stem cell-based therapies could be eclipsed by the deficiency of cells due to the hostile microenvironment as well as the transport and preservation processes.Effective emergency strategies are required to be convenient,biocompatible,and stable.Herein,we assess an emergency cell-free treatment using mesenchymal stem cell-derived exosomes,which have proven capable of comprehensive mitigation of the inhibitory lesion microenvironment.The clinically validated fibrin glue is utilized to encapsulate the exosomes and in-situ gelates in transected rat spinal cords to provide a substrate for exosome delivery as well as nerve tissue growth.The emergency treatment alleviates the inflammatory and oxidative microenvironment,inducing effective nerve tissue repair and functional recovery.The therapy presents a promising strategy for effective emergency treatment of central nervous system trauma。 | Jiafu Mu Jiahe Wu Jian Cao Teng Ma Liming Li Shiqing Feng Jianqing Gao | 2021 | Asian Journal of Pharmaceutical Sciences2021，16，6： | 1 |
| 6 | Study on optical and electrical switching properties and phase transition mechanism of Mo^6+-doped vanadium dioxide thin films显示文摘 | SHIQING XU HONGPING MA SHIXUN DAI | 2004 | JOURNAL OF MATERIALS SCIENCE2004，39，： | 1 |
| 7 | Study on optical and electrical switching properties and phase transition mechanism of Mo6+-doped vanadium dioxide thin films显示文摘 | Shiqing Xu Hongping Ma Shixun Dai Zhonghong Jiang | 2004 | Journal of Materials Science2004，，2： | 1 |
| 8 | Tm^3+/ Er^3+/Yb^3+-codoped Oxyhalide Tellurite Glasses as Materials for Ghree-dimensional display显示文摘 | Shiqing Xu Hongping Ma Daiwei Fang | 2005 | Materials letters2005，59，2425： | 1 |
| 9 | Tm^3+/Er^3+/Yb^3+ -codoped oxyhalide tellurite glasses as materials for three-dimensional display显示文摘 | Shiqing Xu Hongping Ma Dawei Fang | 2005 | Mater Lett2005，59，： | 1 |
| 10 | Study on Optical and Electrical Swithing Properties and Phase Transition Mechanism of Mo^6+ - doped Vanadium Dioxide Thin Film 显示文摘 | Xu Shiqing Ma Hongping Zhao Kang | 2004 | Material Science2004，39，： | 1 |
| 11 | Variable threshold concept lattices显示文摘 | Wenxiu Zhang Jianmin Ma Shiqing Fan | | 0，，： | 1 |
| 12 | Synthesis and luminescence properties of glass ceramics containing MSiO 3 :Eu 2+ (M=Ca, Sr, Ba) phosphors for white LED显示文摘 | Zhiguang Cui Guohua Jia Degang Deng Youjie Hua Shilong Zhao Lihui Huang Huanping Wang Hongping Ma Shiqing Xu | 2011 | Journal of Luminescence2011，，1： | 1 |
| 13 | Study on opti- cal and electrical swithing properties and phase transition mechanism of Mo6+- doped vanadium dioxide thin film 显示文摘 | Xu Shiqing Ma Hongping Zhao Kang | 2004 | Mater Sci2004，39，： | 1 |
| 14 | Effect of ultraviolet blood irradiation and oxygenation on nerve function and function of the red blood cell membrane pump in patients with acute cerebral infarction显示文摘BACKGROUND: Ultraviolet blood irradiation and oxygenation (UBIO) has obtained better clinical effect in treating acute cerebral infarction, but the mechanism underlying this effect remains unclear. OBJECTIVE: To observe the effect of UBIO on the nerve function and activities of K+-Na+-ATPase and Ca2+-Mg2+-ATPase activities on the red blood cell (RBC) membrane of patients with acute cerebral infarction. DESIGN: A randomized and controlled study. SETTING: Department of Neurology, Xiangfan Central Hospital. PARTICIPANTS: From January 2000 to December 2001, excluding those above 70 years old, 58 cases of 700 patients with acute cerebral infarction admitted in the Department of Neurology, Xiangfan Central Hospital, were recruited and divided into two groups according to the random number table: UBIO treated group (n=28), including 17 males and 11 females, aged 40-68 years; and control group (n=30), including 20 males and 10 females, aged 44-69 years. All the patients agreed to participate in the therapeutic program and detected items. The general informations were comparable without obvious differences between the two groups (P > 0.05). METHODS: ① The patients in both groups received routine treatments, besides, those in the UBIO treated group were given UBIO treatment by using the XL-200 type therapeutic apparatus produced in Shijiazhuang, whose ultraviolet wave was set at 253.7 nm with the energy density of 0.568 J/m2 per second, UBIO treatment started from the second day after admission, once every other day, with a single course consisting of 5-7 treatments. ② In the UBIO treated group, the venous blood was sampled before and after the first, third and the completion of the treatment course respectively, the venous blood was taken at each corresponding time point in the control group. After centrifugation of the blood at 10 000 rounds per minute, the RBC membrane was separated and then the activities of K+-Na+-ATPase and Ca2+-Mg2+-ATPase were detected by means of phosphorus determination. ③ The nerve function was scored before and after treatment in both groups with European stroke scale, which included 13 items, the total score was 0-100 points, the higher the score, the better the nerve function. MAIN OUTCOME MEASURES: ① Score of European stroke scale before and after treatment in both groups. ② Comparison of the activities of K+-Na+-ATPase and Ca2+-Mg2+-ATPase on RBC membrane between the two groups before treatment and after the first, third and the completion of the treatment. RESULTS: All the 58 patients with cerebral infarction were involved in the analysis of results. ① The score of European stroke scale had no obvious difference between the two groups [(49.31±11.48), (50.58±12.63), P > 0.05], and it was obviously higher in the UBIO treated group than in the control group after treatment [84.66±13.75), (77.05±11.17), P < 0.05]. ② The activity of K+-Na+-ATPase on RBC membrane in the UBIO treated group was significantly increased after the first and third treatment as compared with before treatment [(31.56±19.25), (27.64±15.83), (17.67±13.83), P < 0.01], it was still higher after the completion of the treatment than before treatment without obvious difference [(20.86±14.53), P > 0.05]. After the first and third treatment, it was obviously higher in the UBIO treated group than in the control group [19.31±11.88), (17.44±10.42), P < 0.01]. ③ In the UBIO treated group, Ca2+-Mg2+-ATPase activity on RBC membrane significantly increased after the first treatment and remained higher than the pre-treatment level throughout the treatment [(27.49±14.72), (17.41±4.82), P < 0.01]. The activity of Ca2+-Mg2+-ATPase on RBC membrane was markedly higher in the UBIO treated group than in the control group after after the first, third and the completion of treatment respectively [(24.83±12.88), (17.70±5.69); (28.08±13.44), (16.32±5.29); (17.42±6.04), P < 0.05-0.01]. CONCLUSION: The effect of UBIO treatment against acute cerebral infarction may be mediated by the increased K+-Na+ ATPase and Ca2+-Mg2+-ATPase activities on RBC membrane, which enhances the RBC transformation ability so as to lower RBC aggregation and correct high blood viscosity. | Jiaquan Wang Chun Mao Kaifu Ma Shiqing Wang | 2006 | Neural Regeneration Research2006，1，1： | 0 |
| 15 | Improving hole transfer of boron nitride quantum dots modified PDI for efficient photodegradation显示文摘In recent years,organic photocatalyst under visible-light absorption has shown significant potential for solving environmental problems.However,it is still a great challenge for constructing a highly active organic photocatalyst due to the low separation efficiency of photogenerated carriers.Herein,an effective and robust photocatalyst perylene-3,4,9,10-tetracarboxylic diamide/boron nitride quantum dots(PDI/BNQDs),consisting of selfassemble PDI withπ–πstacking structure and BNQDs,has been constructed and researched under visible light irradiation.The PDI/BNQDs composite gradually increases organic pollutant photodegradation with the loading amount of BNQDs.With 10 mL of BNQDs solution added(PDI/BNQDs-10),the organic pollutant photodegradation performance reaches a maximum,about 6.16 times higher with methylene blue and 1.68 times higher with ciprofloxacin than that of pure PDI supramolecular.The enhancement is attributed to improved separation of photogenerated carriers from self-assembled PDI by BNQDs due to their preeminent ability to extract holes.This work is significant for the supplement of PDI supramolecular composite materials.We believe that this photocatalytic design is capable of expanding organic semiconductors’potential for their applications in photocatalysis. | Shiqing Ma Chundong Peng Zeyu Jia Yanmei Feng Kai Chen Hao Ding Daimei Chen Zhong-Yong Yuan | 2023 | Frontiers of Chemical Science and Engineering2023，17，11： | 0 |
| 16 | Revealing the role of CCoAOMT1: fine-tuning bHLH transcription factors for optimal anther development显示文摘The tapetum,a crucial innermost layer encompassing male reproductive cells within the anther wall,plays a pivotal role in normal pollen development.The transcription factors (TFs) bHLH010/089/091 redundantly facilitate the rapid nuclear accumulation of DYSFUNCTIONAL TAPETUM 1,a gatekeeper TF in the tapetum.Nevertheless,the regulatory mechanisms governing the activity of bHLH010/089/091 remain unknown.In this study,we reveal that caffeoyl coenzyme A O-methyltransferase 1 (CCoAOMT1) is a negative regulator affecting the nuclear localization and function of bHLH010 and bHLH089,probably through their K259 site.Our findings underscore that CCoAOMT1 promotes the nuclear export and degradation of bHLH010 and bHLH089.Intriguingly,elevated CCoAOMT1 expression resulted in defective pollen development,mirroring the phenotype observed in bhlh010 bhlh089 mutants.Moreover,our investigation revealed that the K259A mutation in the bHLH089 protein disrupted its translocation from the nucleus to the cytosol and impeded its degradation induced by CCoAOMT1.Importantly,transgenic plants with the probHLH089::bHLH089^(K259A)construct failed to rescue proper pollen development or gene expression in bhlh010 bhlh089 mutants.Collectively,these findings emphasize the need to maintain balanced TF homeostasis for male fertility.They firmly establish CCoAOMT1 as a pivotal regulator that is instrumental in achieving equilibrium between the induction of the tapetum transcriptional network and ensuring appropriate anther development. | Zesen Lai Jianzheng Wang Ying Fu Menghan Wang Hong Ma Shiqing Peng Fang Chang | 2024 | Science China(Life Sciences)2024，67，3： | 0 |
| 17 | Defect Properties of GaAs by Positron Annihilation显示文摘DefectPropertiesofGaAsbyPositronAnnihilation￥ChenZhiquan;MaLi;LiShiqing;YanHepin;WangShaojie(DepartmentofPhysics,WuhanUnivers... | Chen Zhiquan Ma Li Li Shiqing Yan Hepin Wang Shaojie (Department of Physics, Wuhan University,Wuhan 430072,China) | 1996 | Wuhan University Journal of Natural Sciences1996，1，1： | 0 |
| 18 | The kinase OsSK41/OsGSK5 negatively regulates amylose content in rice endosperm by affecting the interaction between OsEBP89 and OsBP5显示文摘Amylose content(AC) is the main factor determining the palatability, viscosity, transparency, and digestibility of rice(Oryza sativa)grains. AC in rice grains is mainly controlled by different alleles of the Waxy(Wx) gene. The AP2/EREBP transcription factor OsEBP89 interacts with the MYC-like protein OsBP5 to synergistically regulate the expression of Wx.Here, we determined that the GLYCOGEN SYNTHASE KINASE 5(OsGSK5, also named SHAGGY-like kinase 41 [OsSK41]) inhibits the transcriptional activation activity of OsEBP89 in rice grains during amylose biosynthesis. The loss of OsSK41 function enhanced Wx expression and increased AC in rice grains. By contrast, the loss of function of OsEBP89 reduced Wx expression and decreased AC in rice grains. OsSK41 interacts with OsEBP89 and phosphorylates four of its sites(Thr-28,Thr-30, Ser-238, and Thr-257), which makes OsEBP89 unstable and attenuates its interaction with OsBP5. Wx promoter activity was relatively weak when regulated by the phosphomimicvariantOsEBP89E–OsBP5but relatively strong when regulated by the nonphosphorylatable variant OsEBP89A–OsBP5.Therefore, OsSK41-mediated phosphorylation of OsEBP89 represents an additional layer of complexity in the regulation of amylose biosynthesis during rice grain development. In addition, our findings provide four possible sites for regulating rice grain AC via precise gene editing. | Zejun Hu Fuan Niu Peiwen Yan Kai Wang Lixia Zhang Ying Yan Yu Zhu Shiqing Dong Fuying Ma Dengyong Lan Siwen Liu Xiaoyun Xin Ying Wang Jinshui Yang Liming Cao Shujun Wu Xiaojin Luo | 2023 | Journal of Integrative Plant Biology2023，65，7： | 0 |
| 19 | Argatroban promotes recovery of spinal cord injury by inhibiting the PAR1/JAK2/STAT3 signaling pathway显示文摘Argatroban is a synthetic thrombin inhibitor approved by U.S.Food and Drug Administration for the treatment of thrombosis.However,whether it plays a role in the repair of spinal cord injury is unknown.In this study,we established a rat model of T10 moderate spinal cord injury using an NYU Impactor ModerⅢand performed intraperitoneal injection of argatroban for 3 consecutive days.Our results showed that argatroban effectively promoted neurological function recovery after spinal cord injury and decreased thrombin expression and activity in the local injured spinal cord.RNA sequencing transcriptomic analysis revealed that the differentially expressed genes in the argatroban-treated group were enriched in the JAK2/STAT3 pathway,which is involved in astrogliosis and glial scar formation.Western blotting and immunofluorescence results showed that argatroban downregulated the expression of the thrombin receptor PAR1 in the injured spinal cord and the JAK2/STAT3 signal pathway.Argatroban also inhibited the activation and proliferation of astrocytes and reduced glial scar formation in the spinal cord.Taken together,these findings suggest that argatroban may inhibit astrogliosis by inhibiting the thrombin-mediated PAR1/JAK2/STAT3 signal pathway,thereby promoting the recovery of neurological function after spinal cord injury. | Chenxi Zhao Tiangang Zhou Ming Li Jie Liu Xiaoqing Zhao Yilin Pang Xinjie Liu Jiawei Zhang Lei Ma Wenxiang Li Xue Yao Shiqing Feng | 2024 | Neural Regeneration Research2024，19，2： | 0 |
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