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| 1 | PbMC1a/1b regulates lignification during stone cell development in pear(Pyrus bretschneideri)fruit显示文摘Programmed cell death(PCD)and secondary cell wall(SCW)thickening in pear fruit are accompanied by the deposition of cellulose and lignin to form stone cells.Metacaspase is an important protease for development,tissue renewal and PCD.The understanding of the molecular mechanism whereby pear(Pyrus)metacaspase promotes PCD and cell wall lignification is still limited.In this study,the Metacaspases gene family(PbMCs)from P.bretschneideri was identified.PbMC1a/1b was associated with lignin deposition and stone cell formation by physiological data,semiquantitative real-time polymerase chain reaction(RT-PCR)and quantitative RT-PCR(qRT-PCR).Relative to wildtype(WT)Arabidopsis,the overexpression of PbMC1a/1b increased lignin deposition and delayed growth,thickened the cell walls of vessels,xylary fibers and interfascicular fibers,and increased the expression of lignin biosynthetic genes.Yeast two-hybrid(Y2H),bimolecular fluorescence complementation(BiFC)and GST pull-down assays indicated that the PbMC1a/1b protein physically interacted with PbRD21.Simultaneously,the transient expression of PbMC1a/1b and PbRD21 led to significant changes in the expression of genes and lignin contents in pear fruits and flesh calli.These results indicate that PbMC1a/1b plays an important role in cell wall lignification,possibly by interacting with PbRD21 to increase the mRNA levels of some lignin synthesis-associated genes and promote the formation of stone cells in pear fruit. | Xin Gong Zhihua Xie Kaijie Qi Liangyi Zhao Yazhou Yuan Jiahui Xu Weikang Rui Katsuhiro Shiratake Jianping Bao Shahrokh Khanizadeh Shaoling Zhang Shutian Tao | 2020 | Horticulture Research2020，7，1： | 3 |
| 2 | Change in sugar content and sorbitol - and sucrose - related enzyme activities during development of loquat fruit 显示文摘 | Bantog N A Shiratake K Yamaki S | 1999 | Journal Japanese Society Horticulture Science1999，68，5： | 1 |
| 3 | cDNA cloning of NAD-dependent sorbitol dehydrogenase from peach fruit and its expression during fruit development显示文摘 | Yarnada K Niwa N Shiratake K Yamaki S | 2001 | J of Holt Sci & Biotech2001，76，5： | 1 |
| 4 | Molecular cloning of tonoplast water channel of pear fruit and its expression with the development显示文摘 | SHIRATAKE K KOBAE Y SUZUKI Y | 2001 | J Japan Soc Hort Sci2001，70，： | 1 |
| 5 | cDNA cloning of NAD - dependent sorbitol dehydrogenase from peach fruit and its expression during fruit development 显示文摘 | Yamada K Niwa N Shiratake K | 2001 | The Journal of Horticultural Science and Biotechnology2001，76，5： | 1 |
| 6 | Air-drying of cells, the novel conditions for stimulated synthesis of triacylglycerol in a Green Alga, Chlorella kessleri显示文摘 | Shiratake T Sato A Minoda A | 2013 | PloS One2013，8，79： | 1 |
| 7 | Expanding roles of plant aquaporins in plasma membranes and cell or- ganelles 显示文摘 | Katsuhara M Hanba T Y Shiratake K | 2008 | Functional Plant Biology2008，35，： | 1 |
| 8 | cDNA cloning of NAD-dependent sorbitol dehydrogenase from peach fruit and its expression during fruit development显示文摘 | Yamada K Niwa N Shiratake K | 2001 | Journal of Horticulture Science & Biotechnology2001，76，5： | 1 |
| 9 | Cloning of two isoforms of soluble acid invertase of Japanese pear and their expression during fruit development显示文摘 | Kunio Yamada Takuya Kojima Nancy Bantog Tetsuji Shimoda Hitoshi Mori Katsuhiro Shiratake Shohei Yamaki | 2006 | Journal of Plant Physiology2006，，6： | 1 |
| 10 | Changes in the phosphorylation state of sucrose synthase during development of Japanese pear fruit 显示文摘 | Tanase K Shiratake K Morl H | 2002 | Physiologia Plarttarum2002，114，： | 1 |
| 11 | Purification and properties of NAD+-dependent sorbitol dehydrogenase from apple fruit显示文摘 | Oura Y Yamada K Shiratake K | 2000 | Phytochemistry2000，54，6： | 1 |
| 12 | Purification and characterization of two soluble acid invertase isozymes from Japanese pear fruit显示文摘 | HASHIZUME H TANASE1 K SHIRATAKE K | 2003 | Phytochemistry2003，63，2： | 1 |
| 13 | Purification and characterization of a NAD+-dependent sorbitol dehydrogenase from Japanese pear fruit显示文摘 | Oura Y Yamada K Shiratake K Yamaki S | 2000 | Phytochemistry2000，54，6： | 1 |
| 14 | Riskfactorsforpostoperativedeliriumafterliverresectionforhepatocellularcarcinoma 显示文摘 | YoshimuraY KuboS ShirataK | 2004 | WorldJSurg2004，28，98： | 1 |
| 15 | Development of a highly water-soluble peptide-based human neutrophil elastase inhibitor; AE-3763 for treatment of acute organ injury显示文摘 | Inoue Y Omodani T Shiratake R | 2009 | Bioorg Med Chem2009，17，21： | 1 |
| 16 | Changes in the phosphorylation state of sucrose synthase duringdevelopment of Japanese pear fruit 显示文摘 | Tanase K Shiratake K Mori H | 2002 | Physiol Plant2002，114，： | 1 |
| 17 | A proteomic approach to identification of trans-membrane proteins and membrane anchored proteins of Arobidopsis thaliana by peptide sequencing 显示文摘 | SAZUKA T KETA S SHIRATAKE K | 2004 | DNA Research2004，11，： | 1 |
| 18 | cDNA cloning of NADdependent sorbitol dehydrogenase from peach fruit and its expression during fruit development显示文摘 | Yamada K Niwa N Shiratake | 2001 | J of Hort Sci &Biotech2001，76，5： | 1 |
| 19 | eDNA cloning of NAD-dependent sorbitol dehydrogenase from peach fruit and its expression during fruit development 显示文摘 | Yarnada K Niwa N Shiratake K | 2001 | Journal of Horticultural Science and Biotechnology2001，76，5： | 1 |
| 20 | Purification and characterization of a NAD - dependent sorbitol dehydrogenase from Japanese pear fruit 显示文摘 | Oura Y Yamada K Shiratake K | 2000 | Phytochemistry2000，54，： | 1 |
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