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| 1 | Subcellular daunorubicin distribution and its relation to multidrug resistance phenotype in drug-resistant cell line SMMC-7721/R显示文摘AIM: To investigate the correlation between subcellulardaunorubicin distribution and the multidrug resistancephenotype in drug-resistant cell line SMMC-7721/R.METHODS: The multidrug resistant cell line SMMC-7721/R, a human hepatocellular carcinoma cell line,was established. Antisense oligonudeotides (AS-ODN)were used to obtain different multidrug resistancephenotypes by inhibiting the expression of mdr1 geneand/or multidrug resistance-related protein gene(mrp)using Lipofectamine as delivery agent. Expression ofmdr1 and mrp genes was evaluated by RT-PCR andWestern blotting. Intracellular daunorubicin (DNR)concentration was measured by flow cytometry.Subcellular DNR distribution was analyzed by confocallaser scanning microscopy. Adriamycin (ADM) and DMRsensitivity was examined by MTT method.RESULTS: Low level expression of mdr1 and mrp mRNAsand no expression of P-Glycoprotein(P-gp) andmultidrug resistance-related protein (P190) weredetected in parental sensitive cells SMMC-7721/S, butover-expression of these two genes was observed indrug-resistant cell SMMC-7721/R. The expression ofmdr1 and mrp genes in SMMC-7721/R cells was downregulated to the level in the SMMC-7721/S cells by AS-ODN. Intracellular DNR concentration in SMMC-7721/S cells was 10 times higher than that in SMMC-7721/Rcells. In SMMC7721/S cells intracellular DNRdistributed evenly in the nucleus and cytoplasm, whilein SMMC-7721/R cells DNR distributed in a punctatepattern in the cytoplasm and was reduced in thenucleus. DNR concentration in SMMC-7721/R cells co-transfected with AS-ODNs targeting to mdr1 and mrpmRNAs recovered to 25 percent of that in SMMC7721/Scells. Intracellular DNR distribution pattern in drug-resistant cells treated by AS-ODN was similar to drug-sensitive cell, and the cells resistance index (RI) to DNRand ADM decreased at most from 88.0 and 116.0 to 4.0and 2.3, respectively. Co-Transfection of two AS-ODNsshowed a stronger synergistic effect than separatetransfection.CONCLUSIONS: P-gp and P190 are two membersmediating MDR in cell line SMMC7721/R. Intracellulardrug concentration increase and subcellular distributionchange are two important factors in multidrugresistance (MDR) formation. The second factor, drugstransport by P-gp and P190 from cell nucleus to organellin cytoplasm, may play a more important role. | Jia-Yin Yang Hua-You Luo Qi-Yuan Lin Zi-Ming Liu Lu-Nan Yan Department of General Surgery,West China Hospital,Sichuan University,Chengdu 610044,Sichuan Province,China Ping Lin Cancer Research Institution,West China Hospital,Sichuan University,610044,Sichuan Province,China Jie Zhang Department of Confocal Laser Scanning Microscopy,West China Hospital,Sichuan University,610044,Sichuan Province,China Shong Lei Cancer Biotechnological Treatment Center,West China Hospital,Sichuan University,610044,Sichuan Province,China | 2002 | World Journal of Gastroenterology2002，8，4： | 12 |
| 2 | 甲状腺乳头状微小癌的积极监测:实施与困惑显示文摘当前,积极监测被认为是甲状腺乳头状微小癌(PTMC)的替代治疗方法之一。大多数新诊断的甲状腺癌是惰性微小癌,可能是积极监测而非立即手术的较好适应证。对适合积极监测的PTMC患者制定选择标准时,我们必须考虑到许多因素,包括肿瘤的特征、患者和医疗团队。如果可能,PTMC的积极监测应成为前瞻性临床试验的一部分,以确保长期安全性,并确定与PTMC进展相关的临床和(或)分子标记物。本文讨论了关于PTMC手术干预的经验教训,描述了PTMC积极监测的概念、应用、注意事项及困惑和展望。对于选择适当的PTMC患者来说,积极监测是替代立即手术的一种较好方案。 | 褚晓秋 徐书杭 刘超 Shong Young Kee | 2019 | 中华内分泌代谢杂志2019，35，4： | 8 |
| 3 | PREVENTION AND TREATMENT OF REJECTION AFTER SIMULTANEOUS PANCREAS-KIDNEY TRANSPLANTATION显示文摘Objective To explore methods of preventing and reversing rejection after simultaneous pancreas-kidney (SPK) tran- splantation. Methods Seventeen patients underwent SPK transplantation from September 1999 to September 2003 were reviewed retrospectively. Immunosuppression was achieved by a triple drug regimen consisting of cyclosporine, mycophenolate mofteil (MMF), and steroids. Three patients were treated with anti-CD3 monoclone antibody (OKT3, 5 mg·d-1) for induction therapy for a mean period of 5-7 days. One patients received IL-2 receptor antibodies (daclizumab) in a dose of 1 mg·kg-1 on the day of transplant and the 5th day posttransplant. One patient was treated with both OKT3 and daclizumab for induction. Results No primary non-functionality of either kidney or pancreas occurred in this series of transplantations. Function of all the kidney grafts recovered within 2 to 4 days after transplantation. The level of serum creatinine was 94 ± 11 μmol/L on the 7th day posttransplant. One patient experienced the accelerated rejection, resulting in the resection of the pancreas and kidney grafts because of the failure of conservative therapy. The incidence of the first rejection episodes at 3 months was 47.1% (8/17). Only the kidney was involved in 35.3% (6/17); and both the pancreas and kidney were involved in 11.8% (2/17). All these patients received a high-dose pulse of methylprednisone (0.5 g·d-1) for 3 days. OKT3 (0.5 mg·d-1) was administered for 7-10 days in two patients with both renal and pancreas rejection. All the grafts were successfully rescued. Conclusion Rejection, particularly acute rejection, is the major cause influencing graft function in SPK transplantation. Monitoring renal function and pancreas exocrine secretion, and reasonable application of immunosuppressants play im- portant roles in the diagnosis and treatment of rejection. | Lei Yang Yong-feng Liu Shu-rong Liu Gang Wu Jia-lin Zhang Yi-man Meng Shao-wei Shong Gui-chen Li | 2005 | Chinese Medical Sciences Journal2005，20，3： | 3 |
| 4 | Proinflammatory cytokine TNFα promotes HPV-associated oral carcinogenesis by increasing cancer stemness显示文摘High-risk human papillomaviruses(HPVs) are involved in the development of several human cancers, including oropharyngeal squamous cell carcinomas. However, many studies have demonstrated that HPV alone is not sufficient for the oncogenic transformation of normal human epithelial cells, indicating that additional cofactors are required for the oncogenic conversion of HPV-infected cells. Inasmuch as chronic inflammation is also closely associated with carcinogenesis, we investigated the effect of chronic exposure to tumor necrosis factor α(TNFα), the major proinflammatory cytokine, on oncogenesis in two immortalized oral keratinocyte cell lines, namely, HPV16-immortalized and human telomerase reverse transcriptase(h TERT)-immortalized cells. TNFαtreatment led to the acquisition of malignant growth properties in HPV16-immortalized cells, such as(1) calcium resistance,(2)anchorage independence, and(3) increased cell proliferation in vivo. Moreover, TNFα increased the cancer stem cell-like population and stemness phenotype in HPV16-immortalized cells. However, such transforming effects were not observed in h TERTimmortalized cells, suggesting an HPV-specific role in TNFα-promoted oncogenesis. We also generated h TERT-immortalized cells that express HPV16 E6 and E7. Chronic TNFα exposure successfully induced the malignant growth and stemness phenotype in the E6-expressing cells but not in the control and E7-expressing cells. We further demonstrated that HPV16 E6 played a key role in TNFα-induced cancer stemness via suppression of the stemness-inhibiting micro RNAs mi R-203 and mi R-200 c. Overexpression of mi R-203 and mi R-200 c suppressed cancer stemness in TNFα-treated HPV16-immortalized cells. Overall, our study suggests that chronic inflammation promotes cancer stemness in HPV-infected cells, thereby promoting HPV-associated oral carcinogenesis. | Hannah SHong Jonathan Akhavan Sung Hee Lee Reuben HKim Mo KKang No-Hee Park Ki-Hyuk Shin | 2020 | International Journal of Oral Science2020，12，1： | 3 |
| 5 | DJ-1 mediates paraquat-induced dopaminergic neuronal cell death显示文摘 | Hyun Joo Kwon Jun Young Heo Jung Hee Shim Ji Hoon Park Kang Sik Seo Min Jeong Ryu Jeong Su Han Minho Shong Jin H. Son Gi Ryang Kweon | 2011 | Toxicology Letters2011，，： | 2 |
| 6 | The American Thyroid Association and American Association of Clinical Endocrinologists hyperthyroidism and other causes of thyrotoxicosis guidelines:viewpoints from Japan and Korea显示文摘 | Yamashita S Amino N Shong YK | 2011 | Thyroid2011，21，6： | 1 |
| 7 | Regulation of major histocompatibility class II gene expression in FRTL-5 thyrocytes:opposite effects of interferon and methimazole显示文摘 | Montani V Shong M Taniguchi S | 1998 | Endocrinology1998，139，1： | 1 |
| 8 | A study of availability-centered preventive maintenance for multi-component systems显示文摘 | You Tern Tsai Kuo Shong Wang Lin Chang Tsai | 2004 | Reliability Engineering & System Safety2004，84，3： | 1 |
| 9 | Iodide suppression of major histocompatibility class I gene expression in thyroid cells involves enhancer A and the transcription factor NF-kappa B显示文摘 | Taniguchi SI Shong M Giuliani C | 1998 | Mol Endocrinol1998，12，1： | 1 |
| 10 | Phylogeny of hagfish based on the mitochondrial 16S rRNA gene 显示文摘 | Kuo CH Shong Huang Lee SC | 2003 | Mol Phylogenet Evol2003，28，： | 1 |
| 11 | Towards synthetic microbial consortia for bioprocessing 显示文摘 | Shong J Jimenez Diaz MR Collins CH | 2012 | Curt Opin Biotechnol2012，23，5： | 1 |
| 12 | An aggregate constraint method for inequality-constrained least squares problems显示文摘 | PENG J ZHANG H SHONG S | 2006 | Journal of Geodesy2006，79，12： | 1 |
| 13 | Sorafenib in radioactive iodine-refractory, locally advanced or metastatic differentiated thyroid cancer: a randomised, double-blind, phase 3 trial显示文摘 | Marcia S Brose Christopher M Nutting Barbara Jarzab Rossella Elisei Salvatore Siena Lars Bastholt Christelle de la Fouchardiere Furio Pacini Ralf Paschke Young Kee Shong Steven I Sherman Johannes W A Smit John Chung Christian Kappeler Carol Pe?a István Mo | 2014 | The Lancet2014，，： | 1 |
| 14 | EID-1 interacts with orphan nuclear receptor SF-1 and represses its transactivation显示文摘 | Park YY Park KC Shong M | 2007 | Mol Cells2007，24，3： | 1 |
| 15 | Using human-centered design to improve the assault rifle显示文摘 | KUO CHENGLANG YUAN CHENGKANG LIU BOR- SHONG et ai | 2012 | Applied Ergonomics2012，43，6： | 1 |
| 16 | Repair of incomplete simple syndactyly by a web flap on a subcutaneous tissue pedicle显示文摘 | Yao JM Shong JL Sun H | | 0，，： | 1 |
| 17 | Model reference fuzzy adaptive control for uncertain dynamical systems with time delays 显示文摘 | WEN Shong | 2004 | IEEE International Conference on systems Man and Cybernetics2004，6，10： | 1 |
| 18 | Studies on the chemical constitu-ents of the Chinese medical drug, root of Paeonia lactiflora Pall 显示文摘 | Fu F Y Shong T M Xu Z P | 1963 | Acta Pharm Sinica1963，29，10： | 1 |
| 19 | A study on the site vibration for the breakage analysis of glass insulators on the high-speed railway显示文摘 | KIM Y S SHONG K M JEON Y J | 2009 | Power Delivery IEEE Transactions on2009，24，4： | 1 |
| 20 | Repair of incomplete simple syndactyly by a web flap on subcutaneous tissue pediele 显示文摘 | Yao JM Shong JL Sun H | 1997 | Plast Reconstr Surg1997，99，7： | 1 |
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