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您的检索式：作者名="Shukao Liu"
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| 1 | Jiaweisinisan facilitates neurogenesis in the hippocampus after stress damage显示文摘The traditional Chinese medicine Jiaweisinisan has antidepressant effects, and can inhibit hypothalamus-pituitary-adrenal gland axis hyperactivity in stress-induced depression. In this study, rat hippocampal neural precursor cells were cultured in serum-free medium in vitro and a stress damage model was established with 120 μM corticosterone. Cells were treated with 10% (v/v) Jiaweisinisan drug-containing serum and the corticosterone antagonist RU38486. Results of the 3-(4,5-dimethylthiazol-2-yl)-3,5-di-phenytetrazoliumromide assay showed that both Jiaweisinisan drug-containing serum and RU38486 promoted the proliferation of neural precursor cells after corticosterone exposure. Immunofluorescence detection showed that after Jiaweisinisan drug-containing serum and RU38486 treatment, the 5-bromo-2-deoxyuridine/terminal deoxynucleotidyl transferase dUTP nick end labeling ratio in hippocampal neural precursor cells significantly increased, and the apoptotic rates of glial cells reduced, and neuron-like cell differentiation from neural precursor cells significantly increased. Our experimental findings indicate that Jiaweisinisan promotes hippocampal neurogenesis after stress damage. | Lili Wu Chuanlian Ran Shukao Liu Lizhen Liao Yanling Chen Hualei Guo Weikang Wu Can Yan | 2013 | Neural Regeneration Research2013，8，12： | 7 |
| 2 | Xiaoyaosan-containing serum inhibits corticosterone-induced apoptosis in PC12 cells by downregulating [Ca^(2+)]_i overload显示文摘Apoptosis in hippocampal neurons is an important contributor to depression.[Ca2+]i of hippocampal neurons may raise rapidly with increased corticosterone concentrations,leading to apoptosis.The anti-depressant effects of Xiaoyaosan (Free and Easy Wanderer’s Powder) may inhibit apoptosis by down regulating the [Ca2+]i overload.PC12 cells were containing with corticosterone to simulate neuronal injury,dosed with Xiaoyaosan-containing serum,and the following parameters were measured: apoptosis,[Ca2+]i overload,and changes in mitochondrial membrane potential (?ψm) and bioenergetics.High-dose Xiaoyaosan-containing serum (the dose of intragastric administration was 2.633 g herb/mL) not only inhibited morphological injury and cell apoptosis,but also suppressed [Ca2+]i overload induced by corticosterone.Xiaoyaosan also blocked the decrease in mitochondrial membrane potential and adenosine triphosphate levels induced by corticosterone.Xiaoyaosan dose-dependently suppresses corticosterone-induced apoptosis,potentially via inhibiting [Ca2+]i overload,and prevents impaired mitochondrial bioenergetics by maintaining mitochondrial membrane potential and adenosine triphosphate production,providing a putative mechanism for its antidepressant effects. | Lin Li Xuanhong Zhang Tingting Yan Qian Li Shukao Liu Lili Wu Can Yan HongmeiTan | 2010 | Neural Regeneration Research2010，5，18： | 0 |
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