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| 1 | Molecular Cytogenetic Characterization of Wheat-Thinopyrum elongatum Addition,Substitution and Translocation Lines with a Novel Source of Resistance to Wheat Fusarium Head Blight显示文摘Thinopyrum elongatum(2n = 2x = 14,EE),a wild relative of wheat,has been suggested as a potentially novel source of resistance to several major wheat diseases including Fusarium Head Blight(FHB).In this study,a series of wheat(cv.Chinese Spring,CS) substitution and ditelosomic lines,including Th.elongatum additions,were assessed for TypeⅡresistance to FHB.Results indicated that the lines containing chromosome 7E of Th.elongatum gave a high level of resistance to FHB,wherein the infection did not spread beyond the inoculated floret.Furthermore,it was determined that the novel resistance gene(s) of 7E was located on the short-arm(7ES) based on sharp difference in FHB resistance between the two 7E ditelosomic lines for each arm.On the other hand,Th.elongatum chromosomes 5E and 6E likely contain gene(s) for susceptibility to FHB because the disease spreads rapidly within the inoculated spikes of these lines. Genomic in situ hybridization(GISH) analysis revealed that the alien chromosomes in the addition and substitution lines were intact,and the lines did not contain discernible genomic aberrations.GISH and multicolor-GISH analyses were further performed on three translocation lines that also showed high levels of resistance to FHB.Lines TA3499 and TA3695 were shown to contain one pair of wheat-Th. elongatum translocated chromosomes involving fragments of 7D plus a segment of the 7E,while line TA3493 was found to contain one pair of wheat-Th.elongatum translocated chromosomes involving the D- and A-genome chromosomes of wheat.Thus,this study has established that the short-arm of chromosome 7E of Th.elongatum harbors gene(s) highly resistant to the spreading of FHB,and chromatin of 7E introgressed into wheat chromosomes largely retained the resistance,implicating the feasibility of using these lines as novel material for breeding FHB-resistant wheat cultivars. | Shulan Fu Zhenling Lv Bao Qi Xiang Guo Jun Li Bao Liu Fangpu Han | 2012 | Journal of Genetics and Genomics2012，39，2： | 35 |
| 2 | Silencing RhoA inhibits migration and invasion through Wnt/β-catenin pathway and growth through cell cycle regulation in human tongue cancer显示文摘地岬相当或相同的事物基因家庭成员 A (RhoA ) 作为肿瘤的一个批评管理者被识别了好攻击的行为。在 thisstudy,我们在位于有鳞的细胞 carcinomaof 舌头(TSCC ) 的生长,移植,和侵略下面的机制估计了 RhoA 的角色。TSCC 房间线 SCC-4 和 CAL27 击倒的稳定的 RhoA 用 Lentiviral transfection 被完成。房间移植,侵略,和房间增长上的 RhoA 弄空的 Theeffects 是坚定的。TSCC 房间线上的可能的位于决定 Galectin-3 (Gal-3 ) 的表示下面 mechanismof RhoA 弄空被也评估,在 vivo 的 -catenin, andmatrix metalloproteinase-9 (MMP-9 ) 。同时, TSCC 生长的内在的机制被 cyclin D1/2 的分析学习, p21 CIP1/WAF1 , 和 p27 Kip1 蛋白质层次。Immunohistochemical 评价被执行进一步证明 Gal-3 和 -catenin expression.We 的改变发现了那,在与在舌头的人的 TSCC 房间注射的老鼠, RhoA 层次与在正常纸巾的那些相比在主要肿瘤和 metastasizedlymph 节点是更高的。RhoA 的 Silencing 显著地减少了肿瘤生长, Gal-3 的 decreasedthe 层次, -catenin, MMP-9,和 cyclin D1/2,并且增加了 p21 CIP1/WAF1 和 p27 Kip1 。在 vitro, RhoA 击倒也导致了房间移植,侵略,和增长的抑制。我们的数据建议 RhoA 由通过分别地通过房间周期规定表明小径 andcell 增长的 Wnt/-catenin 调整房间移植和侵略在 TSCC 前进起 asignificant 作用。RhoA 可能是 TSCC 的一个新奇治疗学的目标。 | Guoxin Yan Ronghai Zou Zhenggang Chen Bing Fan Zhaoyan Wang Ying Wang Xiaonan Yin Dong Zhang Lei Tong Fang Yang Weina Jiang Wensheng Fu Jiwei Zheng Martin O. Bergo Martin Dalin Jiawei Zheng Shulan Chen Jianhua Zhou | 2014 | Acta Biochimica et Biophysica Sinica2014，46，8： | 7 |
| 3 | Dicentric Chromosome Formation and Epigenetics of Centromere Formation in Plants显示文摘Plant centromeres are generally composed of tandem arrays of simple repeats that form a complex chromosome locus where the kinetochore forms and microtubules attach during mitosis and meiosis. Each chromosome has one centromere region, which is essential for accurate division of the genetic material. Recently, chromosomes containing two centromere regions (called dicentric chromosomes) have been found in maize and wheat. Interestingly, some dicentric chromosomes are stable because only one centromere is active and the other one is inactivated. Because such arrays maintain their typical structure for both active and inactive centromeres, the specification of centromere activity has an epigenetic component independent of the DNA sequence. Under some circumstances, the inactive centromeres may recover centromere function, which is called centromere reactivation. Recent studies have highlighted the important changes, such as DNA methylation and histone modification, that occur during centromere inactivation and reactivation. | Shulan Fu Zhi Gao James Birchler Fangpu Han | 2012 | Journal of Genetics and Genomics2012，39，3： | 5 |
| 4 | Prevalence of polycystic ovary syndrome in women in China: a large community-based study显示文摘 | Rong Li Qiufang Zhang Dongzi Yang Shangwei Li Shulan Lu Xiaoke Wu Zhaolian Wei Xueru Song Xiuxia Wang Shuxin Fu Jinfang Lin Yimin Zhu Yong Jiang Huai L. Feng Jie Qiao | 2013 | Human Reproduction2013，，9： | 4 |
| 5 | Alteration of Terminal Heterochromatin and Chromosome Rearrangements in Derivatives of Wheat-Rye Hybrids显示文摘小麦黑麦增加和替换线和他们的自我子孙在 telomeric heterochromatin 和着丝点揭示了变化。而且,一个有丝分裂地不稳定的 dicentric 染色体和稳定的 multicentric 染色体在一根中国春天帝国的黑麦 3R 增加线的子孙被观察。一个不稳定的 multicentric 染色体在一根 6R/6D 替换线的子孙被发现。包括终端 heterochromatin 的运动和消失的终端 heterochromatin 的激烈的变化发生在小麦黑麦增加线 3R,和 5RS ditelosomic 增加线的子孙。高度稳定的 minichromosomes 在一根 monosomic 4R 增加线,一根 ditelosomic 5RS 增加线和一根 6R/6D 替换线的子孙被观察。有或没有为 telomeric DNA (TTTAGGG ) n 的 FISH 信号, Minichromosomes 源于一根 monosomic 4R 增加线对下一代稳定、能递送。结果显示着丝点和终端 heterochromatin 能深刻地在小麦黑麦混血儿衍生物被改变。 | Shulan Fu Zhenling Lv Xiang Guo Xiangqi Zhang Fangpu Han | 2013 | Journal of Genetics and Genomics2013，40，8： | 3 |
| 6 | ND-FISH-positive oligonucleotide probes for detecting specific segments of rye (Secale cereale L.) chromosomes and new tandem repeats in rye显示文摘Rye(Secale cereale L.)has been widely used to improve wheat(Triticum aestivum L.)cultivars.Oligo probes combined with non-denaturing fluorescence in situ hybridization(ND-FISH)technology provide a convenient and efficient way to identify individual rye chromosomes.However,suitable ND-FISH-positive oligo probes for recognizing specific segments of rye chromosomes are lacking.Five new ND-FISH-positive oligo probes:Oligo-5BL.46,Oligo-5A8080,Oligo-5A8080.1,Oligo-1AL.73,and Oligo-0R3,combined with two previously reported oligo probes,Oligo-44 and Oligo-45,were used in this study.Probes Oligo-5BL.46,Oligo-5A8080 and Oligo-44 produced signals only in intercalary regions of arms 1RS,5RS,and 5RL,respectively.Probe Oligo-5A8080.1 combined with probe Oligo-45 distinguished the intercalary regions of arms 1RS,5RS,and 6RS simultaneously.Oligo-5A8080 and Oligo-5A8080.1 revealed variation in the distribution of 5S rDNA sequences and polymorphism among 5R chromosomes.Probe Oligo-1AL.73 produced signals only on chromosomes 4R and 7R and contributed to the construction of an improved FISH map of chromosome 4RKu and to the confirmation of 4RLKu breakpoints in wheat-rye 4RLKu translocation chromosomes.Oligo-0R3 produced signals in the telomeric and subtelomeric regions of 14 rye chromosomes.These oligo probes also revealed five new tandem repeats in rye.Using the oligo probes reported in this study,the short arms of 1R,5R,and 6R and the long arms of 4R and 7R can be easily discriminated when these chromosomes are broken. | Wei Xi Shuyao Tang Haimei Du Jie Luo Zongxiang Tang Shulan Fu | 2020 | The Crop Journal2020，8，2： | 2 |
| 7 | Identification and Physical Mapping of New PCR-Based Markers Specific for the Long Arm of Rye(Secale cereale L.) Chromosome 6显示文摘To effectively use elite genes on the long arm of rye chromosome 6(the 6RL arm) in wheat breeding programs,precise and fast identification of 6RL chromatin in wheat backgrounds is necessary.PCR-based 6RL-specific markers can facilitate the detection of elite genes on 6RL in wheat breeding.However,only a limited number of 6RL-specific markers have been developed.In the present study.300 new PCR-based 6RL-specific markers were identified using specific length amplified fragment sequencing(SLAF-seq) technology,and were further physically mapped to four regions on the 6RL arm using 6R and 6RL deletion lines.Interestingly,127 of the 300 markers were physically localized to a region from the site between 2.3 and 2.5 to the telomere,the same region where the powdery mildew resistance gene was mapped.In addition,95 of the 300 markers exhibit polymorphisms,which can be used to investigate the diversity of rye 6RL arms.The markers developed in this study can be used to identify given segments of 6RL in wheat backgrounds and accelerate the utilization of elite genes on 6RL in wheat breeding. | Meng Li Zongxiang Tang Ling Qiu Yangyang Wang Shuyao Tang Shulan Fu | 2016 | Journal of Genetics and Genomics2016，43，4： | 2 |
| 8 | Development of Triticum aestivume——Leymus mollis Translocation Lines and Identification of Resistance to Stripe Rust显示文摘Wheat stripe rust,caused by Puccinia striiformis f.sp.tritici,is one of the most widely distributed and destructive fungal diseases worldwide.Since 1995,most Chinese wheat cultivars have lost their stripe rust resistance due to the subsequent emergence of the new races CYR30,CYR31,CYR32,and CYR33(Han et al.,2010).Therefore,it is necessary to seek effective resistance genes and develop new resistance germplasm for wheat resistance breeding. | Haoxun Li Renchun Fan Shulan Fu Bo Wei Shichang Xu Jing Feng Qi Zheng Xianping Wang Fangpu Han Xiangqi Zhang | 2015 | Journal of Genetics and Genomics2015，42，3： | 1 |
| 9 | Formation of the modern scenic city of Hangzhou:towards conservation integrated urban planning,1950s–1990s显示文摘From the 1950s to the 1990s,China underwent a significant transformation of its political system and economy,from socialism to rapid development after the reform and opening up.In the 1980s and 1990s,to meet the demand for housing and urban modernization under the rapid growth of cities,almost all Chinese cities underwent large-scale urban construction,transformation and expansion.With this background of rapid advancement,many cities have lost their traditional urban features and characteristics.However,it was during this period that Hangzhou not only restored the historical scenery of West Lake through multiple projects but also explored overall planning of the city and introduced conservation ideas,making West Lake,which was originally located outside the city,part of the city.This period also shaped the contemporary urban characteristics of Hangzhou with traditional landscape features.To explore Hangzhou’s positive experience and determine the critical junctures and influencing factors,this study observed the entire historical process using morphological methods.Based on a summary of the characteristics of each development stage,this study further focuses on the key issue of how conservation ideas are gradually introduced into urban planning and affect the development process. | Shulan Fu Panpan Jin | 2020 | Built Heritage2020，4，3： | 0 |
| 10 | SOLAR RADIO OBSERVATIONS DURING SOLAR-TERRESTRIAL JOINT-OBSERVATIONS IN MARCH AND AUGUST 1989显示文摘1.The renewing project has been finished in the middle of 1989. A new radio telescope at 10.6 cm-λ has started to work since 16 June,1989.Itscomputer data acquision system for two channels of 1 sec and 1 msec has come to oper-ate in Feburary,1990. | Fu Qijun, Liu Yuying, Li Xiaocong, Wang Shulan, Chen Zhijun, Zhao Bing, Zhai PujiangBeijing Astronomical Observatory, Chinese Academy of Chinese, Beijing 100080, China | 1990 | 天文研究与技术1990，，4： | 0 |
| 11 | Primary intrathoracic liposarcoma:a clinical analysis of 31 cases显示文摘Dear Editor,Liposarcoma is a common soft tissue malignancy in adults.It most commonly occurs in the deep soft tis-sue of the extremities and retroperitoneum.Primary intrathoracic liposarcoma is rare,represents 2.7%of all liposarcomas[1].According to the distinct tumor loca-tions,it can be divided into mediastinal,pleural,and pulmonary liposarcomas.Most literature has reviewed the clinicopathological or molecular cytogenetic char-acteristics of mediastinal and thoracic liposarcomas[2,3].However,intrathoracic liposarcoma is poorly docu-mented because of its rarity.Complete surgical resection is thought to be the best treatment for primary intratho-racic liposarcoma,but the impact of radiotherapy and systemic therapy remains unclear.Therefore,we ana-lyzed the clinicopathological data of 31 patients with primary intrathoracic liposarcoma who were treated in the Cancer Hospital of Chinese Academy of Medical Sci-ences between October 1970 and July 2015 to explore their clinicopathologic features and treatment outcomes(Additional file 1:Table S1). | Zhixue Fu Kun Yang Xu Yang Shulan Chen Wenqing Wang Dongfu Chen Jun Zhao Zhishan Li Qinfu Feng Zongmei Zhou Luhua Wang Shugeng Gao Jun Liang | 2019 | Cancer Communications2019，39，1： | 0 |
| 12 | Research perspective and prospective of additive manufacturing of biodegradable magnesium-based materials显示文摘Biodegradable metals such as magnesium(Mg)and its alloys have attracted extensive attention in biomedical research due to their excellent mechanical properties and biodegradability.However,traditional casting,extrusion,and commercial processing have limitations in manufacturing components with a complex shape/structure,and these processes may produce defects such as cavities and gas pores which can degrade the properties and usefulness of the products.Compared to conventional techniques,additive manufacturing(AM)can be used to precisely control the geometry of workpieces made of different Mg-based materials with multiple geometric scales and produce desirable medical products for orthopedics,dentistry,and other fields.However,a detailed and thorough understanding of the raw materials,manufacturing processes,properties,and applications is required to foster the production of commercial Mg-based biomedical components by AM.This review summarizes recent advances and important issues pertaining to AM of Mg-based biomedical products and discusses future development and application trends. | Qingyun Fu Wenqi Liang Jiaxin Huang Weihong Jin Baisong Guo Ping Li Shulan Xu Paul K.Chu Zhentao Yu | 2023 | Journal of Magnesium and Alloys2023，11，5： | 0 |
| 13 | Formation of City-Lake Integrated Urban Morphology in Hangzhou: A Study on the Related History Starting from the New Market Plan of the Lakefront District in Early Modern Times显示文摘In the early modern times in China, local planners have made several construction plans for Hangzhou's old city center and the West Lake, resulting in the gradual formation of a city-lake integrated urban form, which is valued nowadays for its uniqueness and characteristically Chinese cityscape aesthetics. The key plan that spurred this process of linking the old city with the West Lake was a plan titled 'Building a New Market'(1914). By elucidating the time, process, and contents of the plan, this paper analyzes the spatial transformation of the lakefront districts based on old maps, and then interprets how it led the forming process of the 'city-lake integrated' urban form in Hangzhou. | Fu Shulan Yukio Nishimura Qian Fang | 2015 | China City Planning Review2015，24，2： | 0 |
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