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| 1 | Molecular Cytogenetic Characterization of Wheat-Thinopyrum elongatum Addition,Substitution and Translocation Lines with a Novel Source of Resistance to Wheat Fusarium Head Blight显示文摘Thinopyrum elongatum(2n = 2x = 14,EE),a wild relative of wheat,has been suggested as a potentially novel source of resistance to several major wheat diseases including Fusarium Head Blight(FHB).In this study,a series of wheat(cv.Chinese Spring,CS) substitution and ditelosomic lines,including Th.elongatum additions,were assessed for TypeⅡresistance to FHB.Results indicated that the lines containing chromosome 7E of Th.elongatum gave a high level of resistance to FHB,wherein the infection did not spread beyond the inoculated floret.Furthermore,it was determined that the novel resistance gene(s) of 7E was located on the short-arm(7ES) based on sharp difference in FHB resistance between the two 7E ditelosomic lines for each arm.On the other hand,Th.elongatum chromosomes 5E and 6E likely contain gene(s) for susceptibility to FHB because the disease spreads rapidly within the inoculated spikes of these lines. Genomic in situ hybridization(GISH) analysis revealed that the alien chromosomes in the addition and substitution lines were intact,and the lines did not contain discernible genomic aberrations.GISH and multicolor-GISH analyses were further performed on three translocation lines that also showed high levels of resistance to FHB.Lines TA3499 and TA3695 were shown to contain one pair of wheat-Th. elongatum translocated chromosomes involving fragments of 7D plus a segment of the 7E,while line TA3493 was found to contain one pair of wheat-Th.elongatum translocated chromosomes involving the D- and A-genome chromosomes of wheat.Thus,this study has established that the short-arm of chromosome 7E of Th.elongatum harbors gene(s) highly resistant to the spreading of FHB,and chromatin of 7E introgressed into wheat chromosomes largely retained the resistance,implicating the feasibility of using these lines as novel material for breeding FHB-resistant wheat cultivars. | Shulan Fu Zhenling Lv Bao Qi Xiang Guo Jun Li Bao Liu Fangpu Han | 2012 | Journal of Genetics and Genomics2012，39，2： | 35 |
| 2 | Molecular Cytogenetic Characterization and Stem Rust Resistance of Five Wheat-Thinopyrum ponticum Partial Amphiploids显示文摘Partial amphiploids created by crossing common wheat(Triticum aestivum L.) and Thinopyrum ponticum(Podp.) Barkworth & D.R.Dewey are important intermediates in wheat breeding because of their resistance to major wheat diseases.In this study,we examined the chromosome compositions of five Xiaoyan-series wheat-Th.ponticum partial amphiploids(Xiaoyan 68.Xiaoyan 693,Xiaoyan 784,Xiaoyan 7430,and Xiaoyan 7631) using GISH,multicolor-GISH.and multicolor-FISH.We found several chromosome changes in these lines.For example,wheat chromosomes 1B and 2B were added in Xiaoyan 68 and Xiaoyan 7430,respectively,while wheat chromosome6 B was eliminated from Xiaoyan 693 and Xiaoyan 7631.Chromosome rearrangements were also detected in these amphiploids.including an interspecific translocation involving chromosome 4D and some inlergenomic translocations,such as A-B and A-D translocations,among wheat genomes.Analysis of the Th.ponticum chromosomes in the amphiploids showed that some lines shared the same alien chromosomes.We also evaluated these partial amphiploids for resistance to nine races of stem rust,including TTKSK(commonly known as Ug99).Three lines,Xiaoyan 68,Xiaoyan 784,and Xiaoyan 7430,exhibited excellent resistance to ail nine races,and could therefore be valuable sources of stem rust resistance in wheat breeding. | Qi Zheng Zhenling Lv Zhixia Niu Bin Li Hongwei Li Steven S.Xu Fangpu Han Zhensheng Li | 2014 | Journal of Genetics and Genomics2014，41，11： | 9 |
| 3 | Alteration of Terminal Heterochromatin and Chromosome Rearrangements in Derivatives of Wheat-Rye Hybrids显示文摘小麦黑麦增加和替换线和他们的自我子孙在 telomeric heterochromatin 和着丝点揭示了变化。而且,一个有丝分裂地不稳定的 dicentric 染色体和稳定的 multicentric 染色体在一根中国春天帝国的黑麦 3R 增加线的子孙被观察。一个不稳定的 multicentric 染色体在一根 6R/6D 替换线的子孙被发现。包括终端 heterochromatin 的运动和消失的终端 heterochromatin 的激烈的变化发生在小麦黑麦增加线 3R,和 5RS ditelosomic 增加线的子孙。高度稳定的 minichromosomes 在一根 monosomic 4R 增加线,一根 ditelosomic 5RS 增加线和一根 6R/6D 替换线的子孙被观察。有或没有为 telomeric DNA (TTTAGGG ) n 的 FISH 信号, Minichromosomes 源于一根 monosomic 4R 增加线对下一代稳定、能递送。结果显示着丝点和终端 heterochromatin 能深刻地在小麦黑麦混血儿衍生物被改变。 | Shulan Fu Zhenling Lv Xiang Guo Xiangqi Zhang Fangpu Han | 2013 | Journal of Genetics and Genomics2013，40，8： | 3 |
| 4 | Site-specific transfer of chromosomal segments and genes in wheat engineered chromosomes显示文摘Recently, engineered minichromosomes have been produced using a telomere-mediated truncation technique in some plants. However, the study on transferring genes to minichromosomes is very limited.Here, telomere-mediated truncation was successfully performed in common wheat(Triticum aestivum)to generate stable truncated chromosomes accompanied by a relatively high frequency of chromosomal rearrangements. After the cross between transgenic parents, a promoter-less DsRed gene in a chromosome from one parent was transferred to another chromosome from the other parent at the site behind a maize ubiquitin promoter via the Cre/lox system. DsRed transcripts and red fluorescent proteins were detected in the recombinant plants. In one such seedling, transgenic signals were detected at the centric terminus of chromosome 4D and the distal terminus of chromosome 3A. Clear translocations could be detected at the transgenic loci of these two chromosomes. Intriguingly, signals of centric-specific sequences were co-localized with the translocated D-group chromosomal segment in the terminal region of chromosome 3A. Our results indicate that the Cre/lox system induces the gene swapping to the target chromosome and non-homologous chromosomal recombination simultaneously. These approaches could offer a platform to transfer large DNA fragments or even terminal chromosomal segments to other chromosomes of the natural genome. | Jing Yuan Qinghua Shi Xiang Guo Yalin Liu Handong Su Xianrui Guo Zhenling Lv Fangpu Han | 2017 | Journal of Genetics and Genomics2017，44，11： | 0 |
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