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| 1 | Discrimination of the rare medicinal plant Dendrobium officinale based on naringenin, bibenzyl, and polysaccharides显示文摘The aim of this study was to establish a method for discriminating Dendrobium officinale from four of its close relatives Dendrobium chrysanthum, Dendrobium crystallinum, Dendrobium aphyllum and Dendrobium devonianum based on chemical composition analysis. We analyzed 62 samples of 24 Dendrobium species. High performance liquid chromatography analysis confirmed that the four low molecular weight compounds 4′,5,7-trihydroxyflavanone (naringenin), 3,4-dihydroxy-4′,5-dime- thoxybibenzyl (DDB-2), 3′,4-dihydroxy-3,5′-dimethoxybibenzyl (gigantol), and 4,4′-dihydroxy-3,3′,5-trimethoxybibenzyl (moscatilin), were common in the genus. The phenol-sulfuric acid method was used to quantify polysaccharides, and the monosaccharide composition of the polysaccharides was determined by gas chromatography. Stepwise discriminant analysis was used to differentiate among the five closely related species based on the chemical composition analysis. This proved to be a simple and accurate approach for discriminating among these species. The results also showed that the polysaccharide content, the amounts of the four low molecular weight compounds, and the mannose to glucose ratio, were important factors for species discriminant. Therefore, we propose that a chemical analysis based on quantification of naringenin, bibenzyl, and polysaccharides is effective for identifying D. officinale. | CHEN XiaoMe WANG FangFei WANG YunQiang LI XueLan WANG AiRong WANG ChunLan GUO ShunXing | 2012 | Science China(Life Sciences)2012，55，12： | 25 |
| 2 | Phylogenetic constrains on mycorrhizal specificity in eight Dendrobium(Orchidaceae) species显示文摘Plant phylogeny constrains orchid mycorrhizal(OrM) fungal community composition in some orchids. Here, we investigated the structures of the OrM fungal communities of eight Dendrobium species in one niche to determine whether similarities in the OrM fungal communities correlated with the phylogeny of the host plants and whether the Dendrobium-OrM fungal interactions are phylogenetically conserved. A phylogeny based on DNA data was constructed for the eight coexisting Dendrobium species,and the OrM fungal communities were characterized by their roots. There were 31 different fungal lineages associated with the eight Dendrobium species. In total, 82.98% of the identified associations belonging to Tulasnellaceae, and a smaller proportion involved members of the unknown Basidiomycota(9.67%). Community analyses revealed that phylogenetically related Dendrobium tended to interact with a similar set of Tulasnellaceae fungi. The interactions between Dendrobium and Tulasnellaceae fungi were significantly influenced by the phylogenetic relationships among the Dendrobium species. Our results provide evidence that the mycorrhizal specificity in the eight coexisting Dendrobium species was phylogenetically conserved. | Xiaoke Xing Xueting Ma Jinxin Men Yanhong Chen Shunxing Guo | 2017 | Science China(Life Sciences)2017，60，5： | 10 |
| 3 | Effects of inoculating fungi on agilawood formation in Aquilaria sinensis显示文摘Agilawood is a costly heartwood medicine obtained from Aquilaria sinensis with active ingredients mainly composed of volatile and semi-volatile substances. However, the formation time of agilawood is quite long and little is known about its formation mechanism. Two highly active fungi obtained from natural agilawood were inoculated on A. sinensis trees to understand their interaction processes and elucidate the transformation rules of induced chemical compositions within different test periods. The results demonstrated that the fungi could successfully colonize living tissues and cells and activate the host defense system, resulting in agilawood accumulation. With increasing time, the main components of A. sinensis converted into constituents or analogs of agilawood and the host exhibited 'self-injury' to prevent fungal intrusion and protect other tissues. The data presented here could provide scientific basis for producing agilawood with the two new fungi in a safe, feasible, and sustainable manner without destroying rare Aquilaria plants. | CUI JinLong GUO ShunXing FU ShaoBin XIAO PeiGen WANG MengLiang | 2013 | Chinese Science Bulletin2013，58，26： | 8 |
| 4 | Mycorrhizal fungal community composition in seven orchid species inhabiting Song Mountain, Beijing, China显示文摘Mycorrhizal fungi play an important role in the germination and growth of orchids essentially influencing their survival,abundance, and spatial distribution. In this study, we investigated the composition of the mycorrhizal fungal community in seven terrestrial orchid species inhabiting Song Mountain, Beijing, China, using Illumina MiSeq high-throughput sequencing. The mycorrhizal communities in the seven orchids were mainly composed of members of the Ceratobasidiaceae, Sebacinales, and Tulasnellaceae, while a number of ectomycorrhizal fungi belonging to the Russulaceae, Tricholomataceae, Thelephoraceae, and Cortinariaceae were occasionally observed. However, the dominant fungal associates and mycorrhizal community differed significantly among the orchid species as well as subhabitats. These findings confirm the previous observation that sympatric orchid species show different preferences for mycorrhizal fungi, which may drive niche partitioning and contribute to their cooccurrence. | Yanhong Chen Yue Gao Linli Song Zeyu Zhao Shunxing Guo Xiaoke Xing | 2019 | Science China(Life Sciences)2019，62，6： | 6 |
| 5 | Screening for differentially expressed genes in Anoectochilus roxburghii (Orchidaceae) during symbiosis with the mycorrhizal fungus Epulorhiza sp.显示文摘Mycorrhizal fungi promote the growth and development of plants, including medicinal plants. The mechanisms by which this growth promotion occurs are of theoretical interest and practical importance to agriculture. Here, an endophytic fungus (AR-18) was isolated from roots of the orchid Anoectochilus roxburghii growing in the wild, and identified as Epulorhiza sp. Tissue-cultured seedlings of A. roxburghii were inoculated with AR-18 and co-cultured for 60 d. Endotrophic mycorrhiza formed and the growth of A. roxburghii was markedly promoted by the fungus. To identify genes in A. roxburghii that were differentially expressed during the symbiosis with AR-18, we used the differential display reverse transcription polymerase chain reaction (DDRT-PCR) method to compare the transcriptomes between seedlings inoculated with the fungus and control seedlings. We amplified 52 DDRT-PCR bands using 15 primer combinations of three anchor primers and five arbitrary primers, and nine bands were re-amplified by double primers. Reverse Northern blot analyses were used to further screen the bands. Five clones were up-regulated in the symbiotic interaction, including genes encoding a uracil phosphoribosyltransferase (UPRTs; EC 2.4.2.9) and a hypothetical protein. One gene encoding an amino acid transmembrane transporter was down-regulated, and one gene encoding a tRNA-Lys (trnK) and a maturase K (matK) pseudogene were expressed only in the inoculated seedlings. The possible roles of the above genes, especially the UPRTs and matK genes, are discussed in relation to the fungal interaction. This study is the first of its type in A. roxburghii. | LI Biao TANG MingJuan TANG Kun ZHAO LiFang GUO ShunXing | 2012 | Science China(Life Sciences)2012，55，2： | 6 |
| 6 | Laser-induced damage tests based on a marker-based watershed algorithm with gray control显示文摘An effective damage test method based on a marker-based watershed algorithm with gray control(MWGC) is proposed to study the properties of damage induced by near-field laser irradiation for large-aperture laser facilities.Damage tests were performed on fused silica samples and information on the size of damage sites was obtained by this new algorithm,which can effectively suppress the issue of over-segmentation of images resulting from non-uniform illumination in darkfield imaging.Experimental analysis and results show that the lateral damage growth on the exit surface is exponential,and the number of damage sites decreases sharply with damage site size in the damage site distribution statistics.The average damage growth coefficients fitted according to the experimental results for Corning-7980 and Heraeus-Suprasil312 samples at 351 nm are 1.10 ± 0.31 and 0.60 ± 0.09,respectively. | Yajing Guo Shunxing Tang Xiuqing Jiang Yujie Peng Baoqiang Zhu Zunqi Lin | 2014 | High Power Laser Science and Engineering2014，2，3： | 3 |
| 7 | Effects of mycorrhizal fungus Mycena sp. on the growth and poly-saccharide properties of Dendrobium officinale显示文摘Dear Editor,Dendrobium officinale Kimura et Migo(Orchidaceae),an endangered species,is a medicinal plant and the sole origin plant of Dendrobii Officinalis Caulis(Tiepishihu),a rare Chinese herbal medicine.Several photosynthetic | Xiaomei Chen Haoli Yan Chunlan Wang Lixia Tian Airong Wang Shunxing Guo | 2016 | Science China(Life Sciences)2016，59，9： | 2 |
| 8 | Endophytic fungi from dragon's blood specimen: isolation, identification, phylogenetic diversity and bioactivity显示文摘 | CUI Jinlong GUO Shunxing DONG Hailing | 2011 | Phytotherapy Research2011，25，： | 1 |
| 9 | Isolation and identification of endophytic and mycorrhizal fungi from seeds and roots of Dendrobium ( Orchidaceae ) 显示文摘 | Chen Juan Wang Hui Guo Shunxing | 2012 | Mycorrhiza2012，22，： | 1 |
| 10 | Three new bibenzyl derivatives from Dendrobium candidum显示文摘 | LI Yan WANG Chunlan GUO Shunxing | 2009 | Chem Pharm Bull2009，57，2： | 1 |
| 11 | Three from Dendrobium candidum 显示文摘 | LI Yan WANG Chunlan new bibenzyl derivatives GUO Shunxing | 2009 | Chem Pharm Bull2009，57，2： | 1 |
| 12 | Three guaianolides from Saussurea involucrata and their contents determination by HPLC 显示文摘 | Li Yan Wang Chunlan Guo Shunxing | 2007 | J Pharm Biomed Anal2007，44，： | 1 |
| 13 | The Preparation Technology Research and Composition Analysis of Anti-fatigue Yikangshu Granules显示文摘[Objectives]To optimize the preparation technology of Yikangshu granule,an anti-fatigue health food.[Methods]The process was studied from three aspects(raw material extraction,excipient selection and forming process),and then it was verified in pilot production,and the content of the three components of the product was determined.[Results]The optimum production process of the formula granules was as follows:raw materials were extracted(adding 1∶9 water,extracting twice,2.5 h each time);vacuum concentration;adding excipients and mixing after vacuum drying,crushing and sieving;granulation of 75%ethanol sifted by a 20-mesh sieve;drying at 60℃,sifting through 14-mesh,and packaging.The average content of products obtained was as follows:crude polysaccharides(3204 mg/100 g),total saponins(2295 mg/100 g),astragaloside A(21 mg/100 g).[Conclusions]The optimized preparation technology is stable and feasible,and has a quality that can be controlled,which can provide a reference for the development of health food in food industry. | Kun TANG Tong LIN Chundan ZHANG Yanxia LU Shunxing GUO | 2021 | Medicinal Plant2021，12，5： | 0 |
| 14 | Cloning and expression of three thaumatin-like protein genes from Polyporus umbellatus显示文摘Genes encoding thaumatin-like protein(TLPs) are frequently found in fungal genomes.However, information on TLP genes in Polyporus umbellatus is still limited. In this study, three TLP genes were cloned from P. umbellatus. The full-length coding sequence of PuTLP1, PuTLP2 and PuTLP3 were 768, 759 and 561 bp long, respectively, encoding for 256, 253 and 187 amino acids. Phylogenetic trees showed that P. umbellatus PuTLP1, PuTLP2 and PuTLP3 were clustered with sequences from Gloeophyllum trabeum, Trametes versicolor and Stereum hirsutum, respectively. The expression patterns of the three TLP genes were higher in P. umbellatus with Armillaria mellea infection than in the sclerotia without A. mellea. Furthermore, over-expression of three PuTLPs were carried out in Escherichia coli BL21(DE3) strain, and high quality proteins were obtained using Ni-NTA resin that can be used for preparation of specific antibodies. These results suggest that PuTLP1, PuTLP2 and PuTLP3 in P. umbellatus may be involved in the defense response to A. mellea infections. | Mengmeng Liu Dawei Zhang Yongmei Xing Shunxing Guo | 2017 | Acta Pharmaceutica Sinica B2017，7，3： | 0 |
| 15 | RNA barcode segments for SARS-CoV-2 identification from HCoVs and SARSr-CoV-2 lineages显示文摘Severe acute respiratory syndrome coronavirus 2(SARS-CoV-2),the pathogen responsible for coronavirus disease 2019(COVID-19),continues to evolve,giving rise to more variants and global reinfections.Previous research has demonstrated that barcode segments can effectively and cost-efficiently identify specific species within closely related populations.In this study,we designed and tested RNA barcode segments based on genetic evolutionary relationships to facilitate the efficient and accurate identification of SARS-CoV-2 from extensive virus samples,including human coronaviruses(HCoVs)and SARSr-CoV-2 lineages.Nucleotide sequences sourced from NCBI and GISAID were meticulously selected and curated to construct training sets,encompassing 1733 complete genome sequences of HCoVs and SARSr-CoV-2 lineages.Through genetic-level species testing,we validated the accuracy and reliability of the barcode segments for identifying SARS-CoV-2.Subsequently,75 main and subordinate species-specific barcode segments for SARS-CoV-2,located in ORF1ab,S,E,ORF7a,and N coding sequences,were intercepted and screened based on single-nucleotide polymorphism sites and weighted scores.Post-testing,these segments exhibited high recall rates(nearly 100%),specificity(almost 30%at the nucleotide level),and precision(100%)performance on identification.They were eventually visualized using one and two-dimensional combined barcodes and deposited in an online database(http://gffzz5c123ad9140e4e5fhxb0b0960906q6kxo.ffgz.tsg.suse.edu.cn/).The successful integration of barcoding technology in SARS-CoV-2 identification provides valuable insights for future studies involving complete genome sequence polymorphism analysis.Moreover,this cost-effective and efficient identification approach also provides valuable reference for future research endeavors related to virus surveillance. | Changqiao You Shuai Jiang Yunyun Ding Shunxing Ye Xiaoxiao Zou Hongming Zhang Zeqi Li Fenglin Chen Yongliang Li Xingyi Ge Xinhong Guo | 2024 | Virologica Sinica2024，39，1： | 0 |
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