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| 1 | Epithelial-mesenchymal transition effect of fine particulate matter from the Yangtze River Delta region in China on human bronchial epithelial cells显示文摘Epidemiological studies have demonstrated that fine particulate matter(PM_(2.5)) exposure causes airway inflammation, which may lead to lung cancer. The activation of epithelial–mesenchymal transition(EMT) is assumed to be a crucial step in lung tumor metastasis and development. We assessed the EMT effect of low concentrations(0, 0.1, 1.0, and 5.0 μg/m L)of PM_(2.5) organic extract on a human bronchial epithelial cell line(BEAS-2 B). PM_(2.5) samples were collected from three cities(Shanghai, Ningbo, and Nanjing) in the Yangtze River Delta(YRD) region in autumn 2014. BEAS-2 B cells were exposed to the PM_(2.5) extract to assess cell viability, invasion ability as well as the relative m RNA and protein expressions of EMT markers. Our findings revealed that BEAS-2 B cells changed from the epithelial to mesenchymal phenotype after exposure. In all groups, PM_(2.5) exposure dose-dependently decreased the expression of E-cadherin and increased the expression of Vimentin. The key transcription factors, including ZEB1 and Slug, were significantly up-regulated upon exposure. These results indicated that the PM_(2.5) organic extract induced different degrees of EMT progression in BEAS-2 B cells. The cell invasion ability increased in a concentration-dependent manner after 48 hr of treatment with the extract. This study offers a novel insight into the effects of PM_(2.5) on EMT and the potential health risks associated with PM_(2.5) in the YRD region. | Yulang Chi Qiansheng Huang Yi Lin Guozhu Ye Huimin Zhu Sijun Dong | 2018 | Journal of Environmental Sciences2018，30，4： | 1 |
| 2 | Flos Lonicerae Extracts and Chlorogenic Acid Protect Human Umbilical Vein Endothelial Cells from the Toxic Damage of Perfluorooctane Sulphonate显示文摘 | Liao Yanyan Dong Sijun Kilama R | 2013 | J Inflammation2013，36，3： | 1 |
| 3 | Flos Lonicerae Extracts and Chlorogenic Acid Protect Human Umbilical Vein Endothelial Cells from the Toxic Damage of Perfluorooctane Sulphonate显示文摘 | Liao Yanyan Dong Sijun Kilama R | 2013 | J Inflammation2013，36，3： | 1 |
| 4 | Levels of polychlorinated biphenyls and organochlorine pesticides in edible shellfish from Xiamen (China) and estimation of human dietary intake显示文摘 | Jie Zhang Feng Liu Rongbin Chen Tao Feng Sijun Dong Heqing Shen | 2012 | Food and Chemical Toxicology2012，，12： | 1 |
| 5 | Environmental risk assessment of selected organic chemicals based on TOC test and QSAR estimation models显示文摘Environmental risks of organic chemicals have been greatly determined by their persistence,bioaccumulation, and toxicity(PBT) and physicochemical properties. Major regulations in different countries and regions identify chemicals according to their bioconcentration factor(BCF) and octanol–water partition coefficient(Kow), which frequently displays a substantial correlation with the sediment sorption coefficient(Koc). Half-life or degradability is crucial for the persistence evaluation of chemicals. Quantitative structure activity relationship(QSAR) estimation models are indispensable for predicting environmental fate and health effects in the absence of field-or laboratory-based data. In this study, 39 chemicals of high concern were chosen for half-life testing based on total organic carbon(TOC) degradation,and two widely accepted and highly used QSAR estimation models(i.e., EPI Suite and PBT Profiler) were adopted for environmental risk evaluation. The experimental results and estimated data, as well as the two model-based results were compared, based on the water solubility, Kow, Koc, BCF and half-life. Environmental risk assessment of the selected compounds was achieved by combining experimental data and estimation models. It was concluded that both EPI Suite and PBT Profiler were fairly accurate in measuring the physicochemical properties and degradation half-lives for water, soil, and sediment.However, the half-lives between the experimental and the estimated results were still not absolutely consistent. This suggests deficiencies of the prediction models in some ways, and the necessity to combine the experimental data and predicted results for the evaluation of environmental fate and risks of pollutants. | Yulang Chi Huanteng Zhang Qiansheng Huang Yi Lin Guozhu Ye Huimin Zhu Sijun Dong | 2018 | Journal of Environmental Sciences2018，30，2： | 1 |
| 6 | In situ combined chemical and biological assessment of estrogenic pollution in a water recycling system显示文摘Estrogenic pollution and its control in aquatic systems have drawn substantial attention around the world. The chemical and biological assessment approaches currently utilized in the laboratory or field cannot give an integrated assessment of the pollution when used separately. In this study, in situ chemical and biological methods were combined to detect pollution in a water recycling system. Data for the water quality index(WQI) demonstrated that the water treatment resulted in the decline of pollution from upstream to downstream.Wild male Nile tilapia, Oreochromis niloticus, was sampled in June and September. The concentrations of four common endocrine disrupting chemicals(EDCs) were determined in the tilapia liver by chromatographic analysis methods. The level of 17β-estradiol(E2) declined from upstream to downstream in both months. In contrast, the levels of bisphenol A(BPA),di-(2-ethylhcxyl) phthalate(DEHP), and perfluorooctane sulfonate(PFOS) did not display this declining tendency. The highest relative expression of vitellogenin 1(VTG1) was observed in tilapia from upstream, then the level significantly decreased along the water system. The relative expression levels of CYP1A1 in the water system were also significantly higher than that of the control. However, no declining trend could be observed along the water system. The change of VTG1 expression corresponded well with that of E2 levels in the tilapia liver. Overall,our study assessed the pollution by endocrine disruptors using chemical and biological data with good correspondence. This study also demonstrated the effectiveness of the water recycling system in eliminating estrogen pollution in municipal sewage. | Yulang Chi Qiansheng Huang Huanteng Zhang Yajie Chen Sijun Dong | 2016 | Journal of Environmental Sciences2016，28，5： | 1 |
| 7 | Genetic variants at 10q 23.33 are associated with plasma lipid levels in a Chinese population显示文摘Plasma lipid abnormalities are implicated in the pathogenic process of type 2 diabetes.The IDE-KIF11-HHEX gene cluster on chromosome 10q23.33 has been identified as a susceptibility locus for type 2 diabetes.We hypothesized that genetic variants at 10q23.33 may be associated with plasma lipid concentrations.Seven tagging single nucleotide polymorphisms(SNPs:rs7923837,rs2488075,rs947591,rs11187146,rs5015480,rs4646957 and rs1111875) at 10q23.33 were genotyped in 3,281 subjects from a Han Chinese population,using the TaqMan OpenArray and Sequenom MassARRAY platforms.Multiple linear regression analyses showed that SNP rs7923837 in the 3'-flanking region of HHEX was significantly associated with triglyceride levels(P = 0.019,0.031 mmol/L average decrease per minor G allele) and that rs2488075 and rs947591 in the downstream region of HHEX were significantly associated with total cholesterol levels(P = 0.041,0.058 mmol/L average decrease per minor C allele and P = 0.018,0.063 mmol/L average decrease per minor A allele,respectively).However,the other four SNPs(rs11187146,rs5015480,rs4646957 and rs1111875) were not significantly associated with any plasma lipid concentrations in this Chinese population.Our data suggest that genetic variants in the IDE-KIF11-HHEX gene cluster at 10q23.33 may partially explain the variation of plasma lipid levels in the Han Chinese population.Further studies are required to confirm these findings in other populations. | Sijun Liu Yun Qian Feng Lu Meihua Dong Yudi Lin Huizhang Li Chong Shen Juncheng Dai Yue Jiang Guangfu Jin Zhibin Hu Hongbing Shen | 2014 | The Journal of Biomedical Research2014，28，1： | 1 |
| 8 | Effects of Antisense Oligonucleotides against C-Reactive Protein on the Development of Atherosclerosis in WHHL Rabbits显示文摘 | Qi Yu Zhengcao Liu Ahmed Bilal Waqar Bo Ning Xianghong Yang Masashi Shiomi Mark J. Graham Rosanne M. Crooke Enqi Liu Sijun Dong Jianglin Fan Alexander Szalai | 2014 | Mediators of Inflammation2014，，： | 1 |
| 9 | Levels of polychlorinated biphenyls and organochlorine pesticides in edible shellfish from Xiamen (China) and estimation of human dietary intake显示文摘 | Jie Zhang Feng Liu Rongbin Chen Tao Feng Sijun Dong Heqing Shen | 2012 | Food and Chemical Toxicology2012，，12： | 1 |
| 10 | Synthesis of haptens and production of antibodies to bisphenol A显示文摘Three immunizing haptens of bisphenol A(BPA), including two new haptens, were used to produce highly sensitive and specific polyclonal antibodies. The spacer arms of haptens for coupling to the protein carrier were located at different positions in BPA, and different length spacer arms were tested. Highly sensitive polyclonal antibodies were obtained and characterized using indirect competitive enzyme-linked immunosorbent assay(ic ELISA). Under optimized conditions, the half maximal inhibitory concentration(IC50) value of the best polyclonal antibody was 2.1 mg·L^(-1), based on coating heterogeneous antigens, and this optimal polyclonal antibody was highly sensitive toward BPA and displayed negligible crossreactivity with bisphenol B and bisphenol E. A sensitive ic ELISA method utilizing the polyclonal antibody was developed for the determination of BPA in milk. In spiked samples(5, 10 and 20 mg·L^(-1)), the recovery ranged from 80% to 102% with a coefficient of variation(CV) value below 15.8%. The limit of detection of ic ELISA was1.95 mg·L^(-1). These results indicate that the ic ELISA method is suitable for the detection of BPA in milk. | Xiya ZHANG Xiaoyun DONG Sijun ZHAO Yuebin KE Kai WEN Suxia ZHANG Zhanhui WANG Jianzhong SHEN | 2017 | Frontiers of Agricultural Science and Engineering2017，4，3： | 0 |
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