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您的检索式：作者名="Sinian Xing"
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| 1 | CRISPR/Cas9-introduced single and multiple mutagenesis in strawberry显示文摘CRISPR/Cas9-mediated genome editing is a powerful tool for life science research.Recently,strawberry (Fragaria × ananassa),an important horticultural crop,has emerged as a model organism for investigating the regulatory mechanisms of fruit development and ripening (Shulaev et al.,2011;Jia et al.,2013,2017;Kang et al.,2013;Han et al.,2015).While most cultivated strawberries (e.g.,F.× ananassa)are octoploid,most wild strawberries (e.g.,F.vesca)are diploid.Although CRISPR/Cas9 methods have been established in many plants,the use of this tool in strawberry needs to be further explored (Zhou et al.,2018). | Sinian Xing Meiru Jia Lingzhi Wei Wenwen Mao Usman Ali Abbasi Yaoyao Zhao Yating Chen Minglin Cao Kai Zhang Zhengrong Dai Zhechao Dou Wensuo Jia Bingbing Li | 2018 | Journal of Genetics and Genomics2018，45，12： | 4 |
| 2 | Transient expression of a TaGRF4-TaGIF1 complex stimulates wheat regeneration and improves genome editing显示文摘Genome editing is an unprecedented technological breakthrough but low plant regeneration frequencies and genotype dependence hinder its implementation for crop improvement. Here, we found that transient expression of a complex of the growth regulators TaGRF4 and TaGIF1(TaGRF4-TaGIF1) increased regeneration and genome editing frequency in wheat. When we introduced synonymous mutation in the miR396 target site of TaGRF4, the resulting complex(mTaGRF4-TaGIF1) performed better than original TaGRF4-TaGIF1. Use of m TaGRF4-TaGIF1 together with a cytosine base editor targeting TaALS resulted in 2-9-fold increases in regeneration and transgene-free genome editing in 11 elite common wheat cultivars. Therefore, m TaGRF4-TaGIF1 will undoubtedly be of great value in crop improvement and especially in commercial applications, since it greatly increased the range of cultivars available for transformation. | Fengti Qiu Sinian Xing Chenxiao Xue Jinxing Liu Kunling Chen Tuanyao Chai Caixia Gao | 2022 | Science China(Life Sciences)2022，65，4： | 4 |
| 3 | Shortening the sgRNA-DNA interface enables SpCas9 and eSpCas9(1.1)to nick the target DNA strand显示文摘The length of the sgRNA-DNA complementary sequence is a key factor influencing the cleavage activity of Streptococcus pyogenes Cas9(SpCas9)and its variants.The detailed mechanism remains unknown.Here,based on in vitro cleavage assays and base editing analysis,we demonstrate that reducing the length of this complementary region can confer nickase activity on SpCas9 and eSpCas9(1.1).We also show that these nicks are made on the target DNA strand.These properties encouraged us to develop a dual-functional system that simultaneously carries out double-strand DNA cleavage and C-to-T base conversions at separate targets.This system provides a novel tool for achieving trait stacking in plants. | Rong Fan Zhuangzhuang Chai Sinian Xing Kunling Chen Fengti Qiu Tuanyao Chai Jin-Long Qiu Zhengbin Zhang Huawei Zhang Caixia Gao | 2020 | Science China(Life Sciences)2020，63，11： | 2 |
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