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| 1 | Control of Grain Size and Weight by the OsMKKK10-OsM KK4-OsMAPK6 Signaling Pathway in Rice显示文摘谷物尺寸是在庄稼决定谷物产量的关键农学的特点之一。然而,在庄稼位于谷物尺寸控制下面的机制留下逃犯。这里,我们证明断然表明小径的 OsMKKK10-OsMKK4-OsMAPK6 在米饭调整谷物尺寸和重量。在米饭,当组成地活跃的 OsMKKK10 (CA-OsMKKK10 ) 的 overexpression 导致大、重的谷物,长圆锥花序,和高植物时, OsMKKK10 功能的损失导致小、轻的谷物,短圆锥花序,和半矮子植物。OsMKKK10 交往与并且 phosphorylates OsMKK4。我们识别了 OsMKK4 gain-of-function 异种(large11-1D )? 那生产大、重的谷物。OsMKK4 large11-1D 等位基因编码的 A227T 比 OsMKK4 举办更强壮的 kinase 活动。植物 overexpressing OsMKK4 (OsMKK4-DD ) 的一种组成地活跃的形式也生产大谷物。进一步生物化学、基因的分析表明 OsMKKK10, OsMKK4,和 OsMAPK6 在一条普通小径工作控制谷物尺寸。一起拿,我们的学习在米饭为谷物尺寸和重量的 OsMKKK10-OsMKK4-OsMAPK6 调停串联的控制建立一个重要基因、分子的框架。 | Ran Xu Penggen Duan Haiyue Yu Zhengkui Zhou Baolan Zhang Ruci Wang Jing Li Guozheng Zhang Shangshang Zhuang Jia Lyu Na Li Tuanyao Chai Zhixi Tian Shanguo Yao Yunhai Li | 2018 | Molecular Plant2018，11，6： | 33 |
| 2 | Transient expression of a TaGRF4-TaGIF1 complex stimulates wheat regeneration and improves genome editing显示文摘Genome editing is an unprecedented technological breakthrough but low plant regeneration frequencies and genotype dependence hinder its implementation for crop improvement. Here, we found that transient expression of a complex of the growth regulators TaGRF4 and TaGIF1(TaGRF4-TaGIF1) increased regeneration and genome editing frequency in wheat. When we introduced synonymous mutation in the miR396 target site of TaGRF4, the resulting complex(mTaGRF4-TaGIF1) performed better than original TaGRF4-TaGIF1. Use of m TaGRF4-TaGIF1 together with a cytosine base editor targeting TaALS resulted in 2-9-fold increases in regeneration and transgene-free genome editing in 11 elite common wheat cultivars. Therefore, m TaGRF4-TaGIF1 will undoubtedly be of great value in crop improvement and especially in commercial applications, since it greatly increased the range of cultivars available for transformation. | Fengti Qiu Sinian Xing Chenxiao Xue Jinxing Liu Kunling Chen Tuanyao Chai Caixia Gao | 2022 | Science China(Life Sciences)2022，65，4： | 4 |
| 3 | Shortening the sgRNA-DNA interface enables SpCas9 and eSpCas9(1.1)to nick the target DNA strand显示文摘The length of the sgRNA-DNA complementary sequence is a key factor influencing the cleavage activity of Streptococcus pyogenes Cas9(SpCas9)and its variants.The detailed mechanism remains unknown.Here,based on in vitro cleavage assays and base editing analysis,we demonstrate that reducing the length of this complementary region can confer nickase activity on SpCas9 and eSpCas9(1.1).We also show that these nicks are made on the target DNA strand.These properties encouraged us to develop a dual-functional system that simultaneously carries out double-strand DNA cleavage and C-to-T base conversions at separate targets.This system provides a novel tool for achieving trait stacking in plants. | Rong Fan Zhuangzhuang Chai Sinian Xing Kunling Chen Fengti Qiu Tuanyao Chai Jin-Long Qiu Zhengbin Zhang Huawei Zhang Caixia Gao | 2020 | Science China(Life Sciences)2020，63，11： | 2 |
| 4 | Effect and mechanism of persulfate activated by different methods for PAHs removal in soil显示文摘 | Dan Zhao Xiaoyong Liao Xiulan Yan Scott G. Huling Tuanyao Chai Huan Tao | 2013 | Journal of Hazardous Materials2013，，： | 1 |
| 5 | Indian Mustard Aquaporin Improves Drought and Heavy-metal Resistance in Tobacco显示文摘 | Yuxiu Zhang Zi Wang Tuanyao Chai Zhensong Wen Hongmei Zhang | 2008 | Molecular Biotechnology2008，，3： | 1 |
| 6 | Effect and mechanism of persulfate activated by different methods for PAHs removal in soil显示文摘 | Dan Zhao Xiaoyong Liao Xiulan Yan Scott G. Huling Tuanyao Chai Huan Tao | 2013 | Journal of Hazardous Materials2013，，： | 1 |
| 7 | Efficient shoot regeneration andAgrobacterium-mediated transformation ofBrassica juncea显示文摘 | Zhang Yuxiu Xu Jin Han Lu Wei Wei Guan Ziqiu Cong Lin Chai Tuanyao | 2006 | Plant Molecular Biology Reporter2006，，2： | 1 |
| 8 | Cloning and expression analysis of SKn-type dehydrin gene from bean in response to heavy metals显示文摘 | Yuxiu Zhang Jinmei Li Fei Yu Lin Cong Liyan Wang Gérard Burkard Tuanyao Chai | 2006 | Molecular Biotechnology2006，，3： | 1 |
| 9 | The effects of copper, manganese and zinc on plant growth and elemental accumulation in the manganese hyper accumulator Phyto lacca americana 显示文摘 | Zhao Huijun Wu Liangqi Chai Tuanyao | 2012 | Journal of Plant Physiology2012，169，： | 1 |
| 10 | Nitrate confers rice adaptation to high ammonium by suppressing its uptake but promoting its assimilation显示文摘Dear Editor,earEaltor,Nitrogen(N)is the most important macronutrient driving plant growth and development.For higher plants,inorganic N including nitrate(NO_(3)^(-))and ammonium(NH_(4)^(+))are predominant N sources(Hu et al.,2023).Nitrate needs to be firstly reduced into ammonium to implement its assimilation,thus requiring a higher energy consumption than ammonium,making ammonium more cost effective for plants.However,ammonium usually causes severe growth retardation of plants under high concentration,which is known as ammonium toxicity.The concentrations of nitrate and ammonium greatly vary in different soil environments.Nitrate is the major inorganic N form in dry land,while ammonium accounts for thehighest proportion of inorganic N in the paddy field,where nitrification is suppressed(Haynes and Goh,1978).Although nitrogen is generally one of the most important contributing factors for yield increase,irrational fertilization strategies can cause negative effects. | Yu Yan Zhihua Zhang Huwei Sun Xiujie Liu Junpeng Xie Yahong Qiu Tuanyao Chai Chengcai Chu Bin Hu | 2023 | Molecular Plant2023，16，12： | 0 |
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