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| 1 | One-step base editing in multiple genes by direct embryo injection for pig trait improvement显示文摘The precise and simultaneous acquisition of multiple beneficial alleles in the genome is in great demand for the development of elite pig breeders. Cytidine base editors(CBEs) that convert C:G to T:A have emerged as powerful tools for single-nucleotide replacement. Whether CBEs can effectively mediate C-to-T substitution at multiple sites/loci for trait improvement by direct zygote injection has not been verified in large animals. Here, we determined the editing efficiency of four CBE variants in porcine embryonic fibroblast cells and embryos. The findings showed that hA3A-BE3-Y130F and hA3A-eBE-Y130F consistently resulted in increased base-editing efficiency and low toxic effects in embryonic development. Further, we verified that using a one-step approach, direct zygote microinjection of the CBE system can generate pigs harboring multiple point mutations.Our process resulted in a stop codon in CD163 and myostatin(MSTN) and introduced a beneficial allele in insulin-like growth factor-2(IGF2). The pigs showed disrupted expression of CD163 and MSTN and increased expression of IGF2, which significantly improved growth performance and infectious disease resistance. Our approach allows immediate introduction of multiple mutations in transgene-free animals to comprehensively improve economic traits through direct embryo microinjection,providing a potential new route to produce elite pig breeders. | Ruigao Song Yu Wang Qiantao Zheng Jing Yao Chunwei Cao Yanfang Wang Jianguo Zhao | 2022 | Science China(Life Sciences)2022，65，4： | 9 |
| 2 | Development of an aero-thermal coupled through-flow method for cooled turbines显示文摘The influence of complicated interaction between the flow field and heat transfer in cooled turbines becomes more and more significant with the increasing turbine inlet temperature. However, classical through-flow methods did not take into account the influence of the interaction caused by air cooling. The aerodynamic design and cooling design of cooled turbines were carried out separately, and the iterations between the aerodynamic design and cooling design led to a long design period and raised the design cost. To shorten the design period and decrease the design cost, this paper proposes a concise aero-thermal coupled through-flow method for the design of cooled turbines, taking into account the influence of the complicated interaction between the flow field and heat transfer in cooled turbines. The governing equations, such as energy equation and continuity equation in classical through-flow method are re-derived theoretically by considering the historical influence of cooling with the same method that deals with viscous losses in this paper. A cooling model is developed in this method. The cooled blade is split into a number of heat transfer elements, and the heat transfer is studied element by element along both the span and the chord in detail. This paper applies the method in the design of a two-stage axial turbine, of which the first stator is cooled with convective cooling. With the prescribed blade temperature limitation and the knowledge of the flow variables of the mainstream at the turbine inlet, such as the total pressure, total temperature and mass flow rate, the convergence of the calculation is then obtained and the properties of the flow field, velocity triangles and coolant requirement are well predicted. The calculated results prove that the aero-thermal coupled through-flow method is a reliable tool for flow analysis and coolant requirement prediction in the design of cooled turbines. | GU ChunWei LI HaiBo SONG Yin | 2015 | Science China(Technological Sciences)2015，58，12： | 3 |
| 3 | Design and Analysis of S-CO_2 Cycle and Radial Turbine for SOFC Vehicle Waste-Heat Recovery显示文摘Solid oxide fuel cell(SOFC) vehicles are considered to have broad prospects for development, and the high operating temperature of SOFC results in great potential for waste-heat recovery. Many concepts for utilizing waste heat of SOFC have been suggested and studied, and most of them directly couple an SOFC to a gas turbine, which require the SOFC to operate at an elevated pressure and make the system less flexible and thus harder to operate. In recent years, with the development of turbine and heat exchanger technology, the supercritical carbon dioxide(S-CO_2) power cycle has raised widespread attractions for the waste recovery. This study explores the potential of S-CO_2 Brayton cycle to realize waste-heat recovery for an SOFC vehicle. The SOFC can operate at atmospheric pressure, and the hybrid system is easier to operate than the directly coupled systems. In this paper, a simple recuperated S-CO_2 Brayton cycle is proposed and the key component, radial inflow turbine is designed and focused. The flow state of the designed turbine is analyzed in detail based on computational fluid dynamics(CFD) numerical simulation. Five cases with different impeller tip clearances are numerically simulated to study its influence on the turbine performance. In addition, off-design performance analysis of the radial inflow turbine is conducted considering the temperature fluctuation of SOFC in practical applications. | XIA Liu LI Xuesong SONG Jian REN Xiaodong GU Chunwei | 2019 | Journal of Thermal Science2019，28，3： | 3 |
| 4 | Neutralization of interleukin-17A delays progression of silica-induced lung inflammation and fibrosis in C57BL/6 mice显示文摘 | Ying Chen Cuiying Li Dong Weng Laiyu Song Wen Tang Wujing Dai Ye Yu Fangwei Liu Ming Zhao Chunwei Lu Jie Chen | 2013 | Toxicology and Applied Pharmacology2013，，： | 1 |
| 5 | Gradient histogram estimation and preservation for texture enhanced image denoising显示文摘 | Zuo Wangmeng Zhang Lei Song Chunwei | 2014 | IEEE Trans on Image Processing2014，23，6： | 1 |
| 6 | A new approach to stability analysis of discrete-time recurrent neural networks with time-varying delay 显示文摘 | SONG Chunwei GAO Huijun ZHENG Weixing | 2009 | Neurocomputing2009，72，1012： | 1 |
| 7 | Gradi- ent histogram estimation and preservation for texture en- hanced image denoising 显示文摘 | Zuo Wangmeng Zhang Lei Song Chunwei | 2014 | IEEE Transactions on Image Processing2014，23，6： | 1 |
| 8 | A harlequin ichthyosis pig model with a novel ABCA12 mutation can be rescued by acitretin treatment显示文摘Harlequin ichthyosis (HI) is a severe genetic skin disorder and caused by mutation in the ATP-binding cassette A12 (ABCA12) gene. The retinoid administration has dramatically improved long-term survival of HI, but improvements are still needed. However, the ABCA12 null mice failed to respond to retinoid treatment, which impedes the development of novel cure strategies for HI. Here we generated an ethylnitrosourea mutagenic HI pig model (named Z9), which carries a novel deep intronic mutation IVS49-727 A>G in the ABCA12 gene, resulting in abnormal mRNA splicing and truncated protein production. Z9 pigs exhibit significant clinical symptom as human patients with HI. Most importantly, systemic retinoid treatment significantly prolonged the life span of the mutant pigs via improving epidermal maturation, decreasing epidermal apoptosis, and triggering the expression of ABCA6. Taken together, this pig model perfectly resembles the clinical symptom and molecular pathology of patients with HI and will be useful for understanding mechanistic insight and developing therapeutic strategies. | Xiao Wang Chunwei Cao Yongshun Li Tang Hai Qitao Jia Ying Zhang Qiantao Zheng Jing Yao Guosong Qin Hongyong Zhang Ruigao Song Yanfang Wang Guanghou Shui Sin Man Lam Zhonghua Liu Hong Wei Anming Meng Qi Zhou Jianguo Zhao | 2019 | Journal of Molecular Cell Biology2019，11，12： | 1 |
| 9 | A waterproof and breathable Cotton/rGO/CNT composite for constructing a layer-by-layer structured multifunctional flexible sensor显示文摘Developing a cotton fabric sensing layer with good waterproofness and breathability via a low-cost and eco-friendly method is increasingly important for the construction of comfortable and wearable electronic devices.Herein,a waterproof and breathable cotton fabric composite decorated by reduced graphene oxide(rGO)and carbon nanotube(CNT),Cotton/rGO/CNT,is reported by a facile solution infiltration method,and we adopt such Cotton/rGO/CNT composite to develop a layer-by-layer structured multifunctional flexible sensor,enabling the high-sensitivity detection of pressure and temperature stimulus.Particularly,the multifunctional flexible sensor exhibits a high response toward tiny pressure,demonstrating salient superiority in the continuous and reliable monitoring of human physiological information.Concerning temperature sensing,a good linear response for the temperatures ranging from 28 to 40℃ is achieved by the multifunctional flexible sensor and gives rise to be successfully applied to the non-contact real-time monitoring of human respiration signal.Finally,an array consisting of multifunctional flexible sensors further demonstrates its feasibility in perceiving and mapping the pressure and temperature information of contact objects.This work provides a feasible strategy for designing cotton-based sensing layers that can effectively resist liquid water penetration and allow water vapor transmission,and offers reasonable insight for constructing comfort and multifunctional wearable electronics. | Feifei Yin Yunjian Guo Hao Li Wenjing Yue Chunwei Zhang Duo Chen Wei Geng Yang Li Song Gao Guozhen Shen | 2022 | Nano Research2022，15，10： | 1 |
| 10 | A review of mobile robot motion planning methods:from classical motion planning workflows to reinforcement learning-based architectures显示文摘Motion planning is critical to realize the autonomous operation of mobile robots.As the complexity and randomness of robot application scenarios increase,the planning capability of the classical hierarchical motion planners is challenged.With the development of machine learning,the deep reinforcement learning(DRL)-based motion planner has gradually become a research hotspot due to its several advantageous feature.The DRL-based motion planner is model-free and does not rely on the prior structured map.Most importantly,the DRL-based motion planner achieves the unification of the global planner and the local planner.In this paper,we provide a systematic review of various motion planning methods.Firstly,we summarize the representative and state-of-the-art works for each submodule of the classical motion planning architecture and analyze their performance features.Then,we concentrate on summarizing reinforcement learning(RL)-based motion planning approaches,including motion planners combined with RL improvements,map-free RL-based motion planners,and multi-robot cooperative planning methods.Finally,we analyze the urgent challenges faced by these mainstream RLbased motion planners in detail,review some state-of-the-art works for these issues,and propose suggestions for future research. | DONG Lu HE Zichen SONG Chunwei SUN Changyin | 2023 | Journal of Systems Engineering and Electronics2023，34，2： | 0 |
| 11 | Amorphous gallium oxide homojunction-based optoelectronic synapse for multi-functional signal processing显示文摘In the era of accelerated development in artificial intelligence as well as explosive growth of information and data throughput,underlying hardware devices that can integrate perception and memory while simultaneously offering the bene-fits of low power consumption and high transmission rates are particularly valuable.Neuromorphic devices inspired by the human brain are considered to be one of the most promising successors to the efficient in-sensory process.In this paper,a homojunction-based multi-functional optoelectronic synapse(MFOS)is proposed and testified.It enables a series of basic electri-cal synaptic plasticity,including paired-pulse facilitation/depression(PPF/PPD)and long-term promotion/depression(LTP/LTD).In addition,the synaptic behaviors induced by electrical signals could be instead achieved through optical signals,where its sen-sitivity to optical frequency allows the MFOS to simulate high-pass filtering applications in situ and the perception capability integrated into memory endows it with the information acquisition and processing functions as a visual system.Meanwhile,the MFOS exhibits its performances of associative learning and logic gates following the illumination with two different wave-lengths.As a result,the proposed MFOS offers a solution for the realization of intelligent visual system and bionic electronic eye,and will provide more diverse application scenarios for future neuromorphic computing. | Rongliang Li Yonghui Lin Yang Li Song Gao Wenjing Yue Hao Kan Chunwei Zhang Guozhen Shen | 2023 | Journal of Semiconductors2023，44，7： | 0 |
| 12 | High-throughput sequencing detection and ensartinib treatment of lung cancer harboring NTRK1 fusion显示文摘Dear Editor,Although fusion events involving neurotrophic receptor tyrosine kinase 1,2,and 3 genes(NTRK1,NTRK2,and NTRK3,encoding TRKA/B/C respectively)were found in diverse tumor types,only 0.1%-0.3%of lung cancer patients harbor an NTRK(and mostly NTRK1)fusion as the primary oncogenic event[1].Such low prevalence may be partially due to the limited availability of first-line assays for detecting rare fusion events[2].Immunohistochemistry is limited by sensitivity and variable tissue background,and fluorescence in-situ hybridization falls short of elucidating functional significance such as the identity of the partner or structure of the transcript.While DNA next-generation sequencing(NGS)is suitable for mutation calling(single nucleotide variation[SNV]and insertion-or-deletion[indel]),and RNA NGS is particularly effective in detecting fusions[3],routinely performing these two assays together is labor-intensive.As a solution,we have developed a single NGS assay(PANO-Seq)for unified RNA/DNA target enrichment library preparation[4],which takes about 12 hours and $10 to prepare. | Zhengbo Song Chunwei Xu Xingxiang Pu Youcai Zhu Wenxian Wang Xingliang Li Yanqiu Gao Wenliang Zhu Yunwei He Lin Wu Li Mao Li Chen Ming Chen | 2021 | Cancer Communications2021，41，2： | 0 |
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