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| 1 | Casein kinase Ⅱ interacts with prion protein in vitro and forms complex with native prion protein in vivo显示文摘最必要、关键的步在能递送的海绵状的 encephalopathy 的致病期间是到病理学的 isoform 的细胞的 prion 蛋白质的 conformational 变化。酷蛋白 kinase II (CK2 ) 是一无所不在地表示了并且 evolutionarily 保存了为生存能力是必要的多种的蛋白质 kinase。探索在 CK2 和 prion 蛋白质(PrP ) 之间的可能的分子的相互作用,人的 CK2α 和 CK2β 的全身的序列互补 DNA 作为模板从房间线 SH-SY5Y 用彻底的送信人 RNA 与反向的抄写聚合酶链反应被放大;然后,熔化蛋白质 histidine-CK2α 和谷胱甘肽 S-transferase-histidine-CK2β 在 Escherichia 关口 i 被表示。在 CK2 和 PrP 之间的相互作用与 co-immunoprecipitation 和下拉试金被评估。结果证明 recombinant PrP 与 CK2α ,然而并非与 CK2β 明确地跳了。在仓鼠大脑的本国的 CK2 和 PrP 与对方一起交往了,形成蛋白质建筑群。从正常大脑的 PrP (diglycosylated, monoglycosylated 和 unglycosylated PrP ) 的三种不同 glycosylated 形式与 CK2α 子单元交往了,尽管 unglycosylated PrP 似乎与 CK2α 子单元有一个更强壮的有约束力的能力。负责与 CK2α 交往的领域在 PrP 的 C 终端片断被定位(残余 91 231 ) 。这研究为在 PrP 和 CK2α 之间的分子的相互作用建议了可靠试验性的数据(两个在 recombinant 和本国的范畴) ,为进一步估计 PrP-CK2 相互作用的潜在的生物意义的科学线索,和在 prion 疾病的致病的 CK2 的可能的角色。 | Jianming Chen Chen Gao Qi Shi Guirong Wang Yanjun Lei Bing Shan Baoyun Zhang Chenfang Dong SongShi Xin Wang Chan Tian Jun Han Xiaoping Dong | 2008 | Acta Biochimica et Biophysica Sinica2008，40，12： | 3 |
| 2 | Abnormal expression of vascular endothelial growth factor (VEGF) and its clinical features in tissues of human lung cancer显示文摘Objective: Angiogennesis, the formation of new blood vessels from the existing vascular bed, is essential step for growth and invasion of primary tumor. Vascular endothelial growth factor (VEGF) is known to play crucial role in tumor angiogenesis. In the present study, we investigate the expression of VEGF and VEGF-mRNA in the angiogennesis, metastasis and prognosis of lung cancer. Methods: The VEGF cellular distributions and expression in 38 specimens of patients with lung cancer were investigated with immunohistochemistry stain technology. The total RNAs in 38 tissues of lung cancer was measured, then the levels of VEGF-mRNA expression were analyzed by a reverse-transcription polymerase chain reaction (RT-PCR) assay. The levels of VEGF in sera of patients with lung cancer, benign lung diseases and healthy controls were detected through Enzyme linked immunosorbent assay (ELISA) method. Results: The VEGF positive stain was 76% in 38 cases of lung cancer specimens. The 89% rate of VEGF stain was found for clinical stage Ⅲ cases and 92% for stage Ⅳ lung cancers. The significantly higher expression of VEGF was evidenced in patients with lymph node metastasis (84%), distant metastasis (90%), and lung cancers with lower histological differentiation (89%), respectively. The expression level of total RNA was significantly higher in patients with lung cancers than that in their paracancerous or distant lung tissues. The VEGF expressions were tightly correlated with total RNA concentration of lung carcinoma (P<0.01). The predominant expressions of VEGF121 and VEGF165 gene fragments were found in lung cancer specimens by RT-PCR analysis. No significant difference of serum VEGF levels was detected between cases with lung cancer and patients with benign diseases. However, the VEGF level of cases with benign diseases was decreased significantly after patients with anti-inflammation medication. Conclusion: The present data suggested that the tumor tissue VEGF expression and VEGF-mRNA analysis in patients with lung cancer be a useful indicator for angiogenesis and metastasis of lung cancer. | Xinhua Wu Dengfu Yao Gongshen Shi Liwei Qiu Wei Wu Songshi Ni Xueguang Zhang | 2005 | Journal of Nanjing Medical University2005，19，4： | 3 |
| 3 | Dexamethasone reduces sensitivity to cisplatin by blunting p53-dependent cellular senescence in non-small cell lung cancer显示文摘 | Haiyan Ge Songshi Ni Xingan Wang | | 0，，12： | 1 |
| 4 | Synthesisofmaleicacidfromrenewableresources:catalyticoxidationoffurfuralonliquidmediawithdioxygen显示文摘 | SongShi HuajunGuo GuochuanYin | 2011 | CatalysisCommunications2011，12，： | 1 |
| 5 | 显示文摘 | TANGZhi—Yuan(唐致远) SONGShi—Dong(宋世栋) LIUJian—Hua(刘建华) eta1 | 2003 | 物理化学学报2003，19，9： | 1 |
| 6 | Pollutional haze and COPD : etiolo- gy epidemiology pathogenesis pathology biological markers and ther- apy显示文摘 | Wang Fei Ni Songshi Liu Hua | 2016 | Journal of Thoracic Disease2016，8，1： | 1 |
| 7 | The leaf epidermis of Helminthostachyaceae and Ophioglossaceae[J]显示文摘 | JIAOY(焦瑜) CHENLQ(陈立群) SONGSHY(宋书银) | | 木本植物研究0，，： | 1 |
| 8 | Response of human non-small-cell lung cancer cells to the influence of Wogonin with SGK1 dynamics显示文摘 | Guanglin Shi Qian Wang Xiaoyu Zhou Jun Li Hua Liu Jun Gu Haiying Wang Yi Wu Liang Ding Songshi Ni Zhiyuan Tang | 2017 | Acta Biochimica et Biophysica Sinica2017，49，4： | 1 |
| 9 | The Program Construction Method of Navigation Format Files GPX and KML Based on Geological Exploration Point Information显示文摘GPX and KML are open and multi-purpose,which are widely used in many fields such as basic geological survey,geochemical survey,geophysical survey,engineering survey and so on.Based on the analysis of the syntax features,basic structure and expression of GPX and KML files,this paper discussed the construction methods of GPX and KML files by taking the construction of navigation files of point information as an example.According to the specifications of GPX and KML files,an automatic construction program of GPX and KML files is designed and compiled,which realizes the automatic generation of batch point navigation files and supports in related software,and shows good effects of holding and displaying,which is helpful to simplify work flow and improve work efficiency. | Songshi XIE | 2021 | Agricultural Biotechnology2021，10，3： | 0 |
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