|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Dynamic tracking of stem cells in an acute liver failure model显示文摘AIM:To investigate a dual labeling technique,which would enable real-time monitoring of transplanted embryonic stem cell(ESC) kinetics,as well as long-term tracking.METHODS:Liver damage was induced in C57/BL6 male mice(n = 40) by acetaminophen(APAP) 300 mg/kg administered intraperitoneally.Green fluorescence protein(GFP) positive C57/BL6 mouse ESCs were stained with the near-infrared fluorescent lipophilic tracer 1,1-dioctadecyl-3,3,3,3-tetramethylindotricarbocyanine iodide(DiR) immediately before transplantationinto the spleen.Each of the animals in the cell therapy group(n = 20) received 5 × 10 6 ESCs 4 h following treatment with APAP.The control group(n = 20) received the vehicle only.The distribution and dynamics of the cells were monitored in real-time with the IVIS Lumina-2 at 30 min post transplantation,then at 3,12,24,48 and 72 h,and after one and 2 wk.Immunohistochemical examination of liver tissue was used to identify expression of GFP and albumin.Plasma alanine aminotransferase(ALT) was measured as an indication of liver damage.RESULTS:DiR-stained ESCs were easily tracked with the IVIS using the indocyanine green filter due to its high background passband with minimal background autofluorescence.The transplanted cells were confined inside the spleen at 30 min post-transplantation,gradually moved into the splenic vein,and were detectable in parts of the liver at the 3 h time-point.Within 24 h of transplantation,homing of almost 90% of cells was confirmed in the liver.On day three,however,the DiR signal started to fade out,and ex vivo IVIS imaging of different organs allowed signal detection at time-points when the signal could not be detected by in vivo imaging,and confirmed that the highest photon emission was in the liver(P < 0.0001).At 2 wk,the DiRsignal was no longer detectable in vivo ;however,immunohistochemistry analysis of constitutively-expressed GFP was used to provide an insight into the distribution of the cells.GFP +ve cells were detected in tissue sections resembling hepatocytes and were dispersed throughout the hepatic parenchyma,with the presence of a larger number of GFP +ve cells incorporated within the sinusoidal endothelial lining.Very faint albumin expression was detected in the transplanted GFP +ve cells at 72 h;however at 2 wk,few cells that were positive for GFP were also strongly positive for albumin.There was a significant improvement in serum levels of ALT,albumin and bilirubin in both groups at 2 wk when compared with the 72 h time-point.In the cell therapy group,serum ALT was significantly(P = 0.016) lower and albumin(P = 0.009) was significantly higher when compared with the control group at the 2 wk time-point;however there was no difference in mortality between the two groups.CONCLUSION:Dual labeling is an easy to use and cheap method for longitudinal monitoring of distribution,survival and engraftment of transplanted cells,and could be used for cell therapy models. | Tarek Ezzat Dipok Kumar Dhar Massimo Malago Steven WM Olde Damink | 2012 | World Journal of Gastroenterology2012，18，6： | 12 |
| 2 | Case of intrahepatic calculi 显示文摘 | VACHELL HR STEVENS WM | 1906 | Br Med J1906，1，2356： | 1 |
| 3 | Diagnostic value of measuring Epstein-Barr virus (EBV) DNA load and carcinoma-specific viral mRNA in relation to antiEBV immunoglobulin A (IgA) and IgG antibody levels in blood of nasopharyngeal carcinoma patients from Indonesia显示文摘 | Stevens S JC Verkuijlen SA WM Hariwiyanto B | 2005 | J Clin Microbiol2005，43，7： | 1 |
| 4 | Liver biopsies from human females contain male hepatocytes in the absence of transplantation显示文摘 | Stevens AM McDonnell WM Mullarkey M | 2004 | Lab Invest2004，84，12： | 1 |
| 5 | Reaction of dinitrogen tetroxide and iodine with olefins and acetylenes 显示文摘 | Stevens Travis E Emmons Wm D | 1958 | Journal of the American Chemical Society1958，80，： | 1 |
| 6 | Case of intrahepatic calculi显示文摘 | Stevens WM | 1906 | Br Med J1906，1，4： | 1 |
| 7 | pulmonary aterial hypertension in sytemic sclerosis:the need for early detcetion and treatment显示文摘 | Proudman SM Stevens WM Sahhar J | 2007 | Intern Med J2007，37，7： | 1 |
| 8 | Broad spectrum antiprotozoal agents that inhibit histone deacetylase:structure-activity relationships of apicidin显示文摘 | Steven LC Robert WM Sandra JDR | 2001 | Bioorg Med Chem Lett2001，11，2： | 1 |
| 9 | Epidemiology of systemic sclerosis显示文摘 | Nikpour M Stevens WM Herrick AL | 2010 | Best Pract Res Clin Rheumatol2010，24，6： | 1 |
| 10 | Network modeling links breast cancer susceptibility and centrosome dysfunction显示文摘 | Pujana MA Han JD Starita LM Stevens KN Tewari M Ahn JS Rennert G Moreno V Kirchhoff T Gold B Assmann V Elshamy WM Rual JF Levine D Rozek LS Gelman RS Gunsalus KC Greenberg RA Sobhian B Bertin N Venkatesan K Ayivi-Guedehoussou N So1é X Hernández P Lázaro C | | 0，，11： | 1 |
| 11 | Human leukocyte antigen class Ⅱ alleles in Caucasian women with primary biliary cirrhosis显示文摘 | Mullarkey ME Stevens AM McDonnell WM | 2005 | Tissue Antigens2005，65，： | 1 |
| 12 | Influence of patient factors on TKA outcomes at 5 to 11 years Follow up显示文摘 | Robert BB Richard WM Steven JM | 2007 | Clin Orthop2007，464，： | 1 |
| 13 | Human leukocyte antigen class Ⅱ alleles in Caucasian women with primary biliary cirrhosis 显示文摘 | Mullarkey ME Stevens AM McDonnell WM | 2005 | Tissue Antigens2005，65，2： | 1 |
| 14 | Pulmonary arterial hypertension in systemic sclerosis: the need for early detection and treatment 显示文摘 | Proudman SM Stevens WM Sahhar J | 2007 | Intern Med J2007，37，7： | 1 |
| 15 | Pulmonary arterial hypertension in systemic sclerosis: the need for early detection and treatment显示文摘 | Proudman SM Stevens WM Sahhar J | 2007 | Inter Med J2007，37，7： | 1 |
| 16 | N-terminal probrain natriuretic peptide in a novel screening algorithm for pulmonary arterial hypertension in systemic sclerosis:a casecontrol study显示文摘 | Thakkar V Stevens WM Prior D | 2012 | Arthritis Res Ther2012，14，3： | 1 |
| 17 | Case of intrahepatic calculi 显示文摘 | Vachell HR Stevens WM | 1906 | Br Med J1906，1，4： | 1 |
| 18 | Humam leukocyte antigen classⅡalleles in Caucasian women with primary biliary cirrhosis显示文摘 | Mullarkey ME Stevens AM McDonnell WM | 2005 | Tissue Antigens2005，65，2： | 1 |
| 19 | Pulmonary arterial hypertension in systemic sclerosis:the need for early detection and treatment显示文摘 | Proudman SM Stevens WM Sahhar J et al | 2007 | Intern Med J2007，37，： | 1 |
| 20 | Case of intrahepatic calculi显示文摘 | Vachell HR Stevens WM | 1906 | British Medical Journal1906，1，： | 1 |
您所在的机构暂未开通此部分的功能，请联系管理员