|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | DNA methylation,microRNAs,and their crosstalk as potential biomarkers in hepatocellular carcinoma显示文摘Epigenetic alterations have been identified as a major characteristic in human cancers.Advances in the field of epigenetics have contributed significantly in refining our knowledge of molecular mechanisms underlying malignant transformation.DNA methylation and microRNA expression are epigenetic mechanisms that are widely altered in human cancers including hepatocellular carcinoma(HCC),the third leading cause of cancer related mortality worldwide.Both DNA methylation and microRNA expression patterns are regulated in developmental stage specific-,cell type specific-and tissue-specific manner.The aberrations are inferred in the maintenance of cancer stem cells and in clonal cell evolution during carcinogenesis.The availability of genome-wide technologies for DNA methylation and microRNA profiling has revolutionized the field of epigenetics and led to the discovery of a number of epigenetically silenced microRNAs in cancerous cells and primary tissues.Dysregulation of these microRNAs affects several key signalling pathways in hepatocarcinogenesis suggesting that modulation of DNA methylation and/or microRNA expression can serve as new therapeutic targets for HCC.Accumulative evidence shows that aberrant DNA methylation of certain microRNA genes is an event specifically found in HCC which correlates with unfavorable outcomes.Therefore,it can potentially serve as a biomarker for detection as well as for prognosis,monitoring and predicting therapeutic responses in HCC. | Sumadi Lukman Anwar Ulrich Lehmann | 2014 | World Journal of Gastroenterology2014，20，24： | 11 |
| 2 | Profile of the breast cancer susceptibility marker rs4245739 identifies a role for miRNAs显示文摘Objective:To determine the influence of the single nucleotide polymorphism(SNP)rs4245739 on the binding and expression of micro RNAs and subsequent MDM4 expression and the correlation of these factors with clinical determinants of ER-negative breast cancers.Methods:Find Tar and miRanda were used to detect the manner in which potential micro RNAs are affected by the SNP rs4245739-flanking sequence.RNA sequencing data for ER-negative breast cancer from The Cancer Genome Atlas(TCGA)were used to compare the expression of miR-184,miR-191,miR-193a,miR-378,and MDM4 in different rs4245739 genotypes.Results:Comparison of ER-negative cancer patients with and without the expression of miR-191 as well as profile micro RNAs(miR-184,miR-191,miR-193a and miR-378 altogether)can differentiate the expression of MDM4 among different rs4245739 genotypes.Although simple genotyping alone did not reveal significant clinical relationships,the combination of genotyping and micro RNA profiles was able to significantly differentiate individuals with larger tumor size and lower number of involved lymph nodes(P<0.05)in the risk group(A allele).Conclusions:We present two novel methods to analyze SNPs within 3′UTRs that use:(i)a single miRNA marker expression and(ii)an expression profile of miRNAs predicted to bind to the SNP region.We demonstrate that the application of these two methods,in particular the miRNA profile approach,permits detection of new molecular and clinical features related to the rs4245739 variant in ER-negative breast cancer. | Sumadi Lukman Anwar Wahyu Wulaningsih Johnathan Watkins | 2017 | Cancer Biology & Medicine2017，14，4： | 3 |
| 3 | hsa-mir-183 is frequently methylated and related to poor survival in human hepatocellular carcinoma显示文摘AIM To screen clinically relevant micro RNAs (mi RNAs) silenced by DNA methylation in human hepatocellular carcinoma(HCC).METHODS Knockdown of DNA methyltransferases (DNMTs) using si RNAs and mi RNA profiling in HCC cell lines were performed to identify DNA hypermethylation-mediated mi RNA downregulation. Confirmation using individual quantitative real-time PCR (qR T-PCR) assays was thenperformed followed by DNA methylation quantification at the promoter of the mi RNA genes. Quantification of DNA methylation and mi RNA expression was then performed in primary HCC tumor samples and related with clinicopathological variables.RESULTS mi RNA profiling after DNMT knockdown in HCC cell lines revealed upregulation of mi R-23, mi R-25 and mi R-183. After q RT-PCR confirmation and Cp G island methylation quantification of these miR NAs in cell lines, further analysis in primary HCC specimens showed that hsa-mi R-183 is hypermethylated in 30% of HCC (n = 40). Expression of mature miR-183 showed an inverse correlation with DNA methylation levels. In HCC cells, DNMT knockdown and 5-aza-2'-deoxycytidine treatment reduced methylation and stimulated expression of mi R-183. In HCC patients, hypermethylation at hsami R-183 promoter significantly correlates with poor survival (log-rank test P = 0.03). DNA methylation analysis in healthy liver, benign liver tumors (hepatocellular adenoma and focal nodular hyperplasia) and their corresponding adjacent tissues showed absence of hypermethylation supporting the notion that aberrant methylation at hsa-miR-183 is specific for the malignant transformation of hepatocytes.CONCLUSION Our data indicate that hypermethylation of hsa-miR-183 is a frequent event in HCC and potentially useful as a novel surrogate diagnostic and prognostic marker. | Sumadi Lukman Anwar Till Krech Britta Hasemeier Elisa Schipper Nora Schweitzer Arndt Vogel Hans Kreipe Reena Buurman Britta Skawran Ulrich Lehmann | 2017 | World Journal of Gastroenterology2017，23，9： | 3 |
| 4 | Differential expression of two genes for sucrose phosphate synthase in sugarcane: molecular cloning of the cDNAs and comparative analysis of gene expression显示文摘 | SUGIHARTO B SAKAKIBAM H SUMADI S T | 1997 | Plant Cell Physiol1997，38，： | 1 |
| 5 | Cesarean section rate: Much room for reduction 显示文摘 | Dabbas M Al Sumadi A | 2007 | Clin Exp Obstet Gynecol2007，34，3： | 1 |
| 6 | Binary Effect of Fly Ash and Palm Oil Fuel Ash on Heat of Hydration Aerated Concrete 显示文摘 | MEHMANNAVAZ T ISMAIL M SUMADI S R | 2014 | Hindawi Publishing Corporation the Scientific World J2014，2014，： | 1 |
| 7 | Differential expression of two genes for sucrose phosphate synthase in sugarcane:molecular cloning of the cDNAs and comparative analysis of gene expression显示文摘 | SUGIHARCO B SAKAKIBARA H SUMADI | 1997 | Plant Cell Physiol1997，38，： | 1 |
| 8 | Concordant hypermethylation of intergenic microRNA genes in human hepatocellular carcinoma as new diagnostic and prognostic marker显示文摘 | Sumadi Lukman Anwar Cord Albat Till Krech Britta Hasemeier Elisa Schipper Nora Schweitzer Arndt Vogel Hans Kreipe Ulrich Lehmann | 2013 | Int J Cancer2013，，3： | 1 |
| 9 | 1997, Diferential ex- pression of two genes for sucrose phosphate synthase in sugarcane: molecular cloning of the cDNAs and compara- tive analysis of gene expression显示文摘 | Sugiharto B Sakakibam H Sumadi S T | 1997 | Plant Cell Physiology1997，38，8： | 1 |
| 10 | Adsorption kinetics and isotherm of Cd(II) ionon Nannochloropsis sp biomass imprinted ionic polymer 显示文摘 | Buhani S Sumadi | 2010 | Desalination2010，259，13： | 1 |
| 11 | Differential expression of two genes for sucrose phosphate synthase in sugarcane:Molecular cloning of the cDNAs and comparative analysis of gene expression显示文摘 | Sugiharto B Sakakibara H Sumadi Sugiyama T | | 0，，： | 1 |
您所在的机构暂未开通此部分的功能，请联系管理员