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您的检索式：作者名="TANGWenhua"
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| 1 | Improving biocontrol activity of Pseudomonas fluorescens through chromosomal inte-gration of 2,4-diacetylphloro-glucinol biosynthesis genes显示文摘Antibiotic 2,4-diacetylphloroglucinol (2,4- DAPG) produced by Pseudomonas fluorescens CPF-10 and 2P24 is a principal factor enabling bacteria to suppress plant diseases caused by soilborne pathogens. In this study, a 2,4-DAPG biosynthesis locus phlACBDE cloned from strain CPF-10 was assembled into a mini-Tn5 transposon and in- troduced into the chromosome of P. fluorescens P32 (2,4- DAPG?), CPF-10 and 2P24 to construct the 2,4-DAPG over- producing derivatives P32-38, CPF10-9 and 2P24-48, respec- tively. All the transgenic strains showed an enhanced anti- biosis capacity against plant microbial pathogens in vitro and two strains, P32-38 and CPF10-9, provided significantly bet- ter protection against wheat take-all disease caused by Gae- umannomyces graminis var. tritici and tomato bacterial wilt caused by Ralstonia solanacearum in greenhouse. Compared to their parental strains, the 2,4-DAPG overproducing de- rivatives colonized to the same extent on the wheat tips in the autoclaved soil, but developed larger populations in natural soil. These results indicated that production of antibiotics 2,4- DAPG by biological control pseudomonads can contribute not only to their disease suppression capacities but also to the ecological competence in the resident microflora. Our re- search also suggests that it is a realistic approach to improve biocontrol capacity of P. fluorescens through the genetic modification of its antibiotic 2,4-DAPG production. | ZHOUHongyou WEIHailei LIUXili WANGYe ZHANGLiqun TANGWenhua | 2005 | Chinese Science Bulletin2005，50，8： | 4 |
| 2 | Diversity analysis for Magnaporthe grisea by Pot2-based PCR显示文摘CO39, its near_isogenic lines, having single blast resistance gene, C101LAC, C101A51, C104PKT, and C101PKT, and its resistance gene pyramid lines BL121, BL241, and A57_119, grew in the blast nursery at IRRI. The seeds were sown in four batches with two weeks interval between batches, using IR50 and IR72 as spread rows and susceptible controls. For moist leaf, spraying water was proceeded every two hours from 8:30 am in sunny days. Blast disease was scored and pathogen was isolated every two weeks. DNA samples of 310 isolates were used for diversity analysis by Pot 2_based PCR (Pot 2, a dispersed retrotransposon of the fungus). | HEYueqiu HeiLeung RobertS.Zeigler TANGWenhua | 2001 | Chinese Rice Research Newsletter2001，9，3： | 0 |
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