|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Molecular Framework for Auxin-Controlled Hlomeostasis-of Shoot Stem Cells in Arabidopsis显示文摘 | Linjie Luo Jian Zengg Haijun Wu Zhaoxia Tian Zhong Zhao | 2018 | Molecular Plant2018，11，7： | 7 |
| 2 | Human CMTM2/CKLFSF2 enhances the ligand-induced transactivation of the androgen receptor显示文摘CKLF (chemokine-like factor)-like MARVEL (MAL and related proteins for vesicle trafficking and mem-brane link domain) transmembrane domain containing (CMTM) is a novel gene family. One member of this family, CMTM2, also named chemokine-like factor superfamily 2 (CKLFSF2), is expressed highly in the testis and moderately in the prostate, marrow and peripheral blood cells. However, the function of human CMTM2 remains unknown. Here, we found that CMTM2 was upregulated in 5α-dihydrotestos- terone (DHT)-treated LNCaP cells. We investigated the relationship between CMTM2 and the androgen receptor. Our results showed that CMTM2 enhanced DHT-mediated androgen receptor (AR) transacti-vation and the expression of prostate specific antigen (PSA). We also observed that CMTM2 enhanced the AR protein level, which was reversed by silencing endogenous CMTM2 expression, which sug-gested that CMTM2 might play an important role in maintaining the AR protein level. We also found that CMTM2 suppressed Akt activation. A previous study showed that Akt could phosphorylate AR at Ser210 and Ser790 and lead to AR ubiquitylation and degradation as well as suppression of AR activity. Taken together, suppressing Akt activation and increasing the AR protein level might be one of the mechanisms for the CMTM2-mediated enhancement of AR transactivation. | LIU DaZhen YIN CaiHua ZHANG YingMei TIAN LinJie LI Ting LI Dan MA DaLong GUO YingLu WANG Ying | 2009 | Chinese Science Bulletin2009，54，6： | 7 |
| 3 | Three-dimensional bioprinted glioblastoma microenvironments model cellular dependencies and immune interactions显示文摘Brain tumors are dynamic complex ecosystems with multiple cell types.To model the brain tumor microenvironment in a reproducible and scalable system,we developed a rapid three-dimensional(3D)bioprinting method to construct clinically relevant biomimetic tissue models.In recurrent glioblastoma,macrophages/microglia prominently contribute to the tumor mass.To parse the function of macrophages in 3D,we compared the growth of glioblastoma stem cells(GSCs)alone or with astrocytes and neural precursor cells in a hyaluronic acid-rich hydrogel,with or without macrophage.Bioprinted constructs integrating macrophage recapitulate patient-derived transcriptional profiles predictive of patient survival,maintenance of stemness,invasion,and drug resistance.Whole-genome CRISPR screening with bioprinted complex systems identified unique molecular dependencies in GSCs,relative to sphere culture.Multicellular bioprinted models serve as a scalable and physiologic platform to interrogate drug sensitivity,cellular crosstalk,invasion,context-specific functional dependencies,as well as immunologic interactions in a species-matched neural environment. | Min Tang Qi Xie Ryan C.Gimple Zheng Zhong Trevor Tam Jing Tian Reilly L.Kidwell Qiulian Wu Briana C.Prager Zhixin Qiu Aaron Yu Zhe Zhu Pinar Mesci Hui Jing Jacob Schimelman Pengrui Wang Derrick Lee Michael H.Lorenzini Deobrat Dixit Linjie Zhao Shruti B.hargava Tyler E.Miller Xueyi Wan Jing Tang Bingjie Sun Benjamin F.Cravatt Alysson R.Muotri Shaochen Chen Jeremy N.Rich | 2020 | Cell Research2020，30，10： | 6 |
| 4 | RNA Methylome Reveals the m^(6)A-mediated Regulation of Flavor Metabolites in Tea Leaves under Solar-withering显示文摘The epitranscriptomic mark N6-methyladenosine(m^(6)A),which is the predominant internal modification in RNA,is important for plant responses to diverse stresses.Multiple environmental stresses caused by the tea-withering process can greatly influence the accumulation of specialized metabolites and the formation of tea flavor.However,the effects of the m^(6)A-mediated regulatory mechanism on flavor-related metabolic pathways in tea leaves remain relatively uncharacterized.We performed an integrated RNA methylome and transcriptome analysis to explore the m^(6)Amediated regulatory mechanism and its effects on flavonoid and terpenoid metabolism in tea(Camellia sinensis)leaves under solar-withering conditions.Dynamic changes in global m^(6)A level in tea leaves were mainly controlled by two m^(6)A erasers(CsALKBH4A and CsALKBH4B)during solar-withering treatments.Differentially methylated peak-associated genes following solarwithering treatments with different shading rates were assigned to terpenoid biosynthesis and spliceosome pathways.Further analyses indicated that CsALKBH4-driven RNA demethylation can directly affect the accumulation of volatile terpenoids by mediating the stability and abundance of terpenoid biosynthesis-related transcripts and also indirectly influence the flavonoid,catechin,and theaflavin contents by triggering alternative splicing-mediated regulation.Our findings revealed a novel layer of epitranscriptomic gene regulation in tea flavor-related metabolic pathways and established a link between the m^(6)A-mediated regulatory mechanism and the formation of tea flavor under solar-withering conditions. | Chen Zhu Shuting Zhang Chengzhe Zhou Caiyun Tian Biying Shi Kai Xu Linjie Huang Yun Sun Yuling Lin Zhongxiong Lai Yuqiong Guo | 2023 | Genomics, Proteomics & Bioinformatics2023，21，4： | 2 |
| 5 | The chromosome-scale genome assembly of Jasminum sambac var. unifoliatum provides insights into the formation of floral fragrance显示文摘Jasmine [Jasminum sambac(L.) Ait.], a tropical and subtropical plant emits a sweet, heady fragrance during flower opening. However, the molecular mechanisms underlying this phenomenon remain largely unknown. In the present study, integrated Illumina sequencing, Pacbio sequencing, and high-throughput chromatin conformation capture(Hi-C) scaffolding was used to generate a 495.60 Mb genome assembly of J.sambac var. unifoliatum cultivar ‘Fuzhou Single-petal’(JSU-FSP), with contig N50 of 16.88 Mb;96.23% of the assembly was assigned to 13 pseudochromosomes. The genome harbors 30 989 protein-coding genes, and 49.47% of the assembled sequences are repetitive sequences. The analysis of duplication modes showed that 51% of genes were duplicated through dispersed duplication, and expanded gene families are mainly involved in photosynthesis, which may be responsible for the light-loving characteristic specific to jasmine. Transcriptome analysis revealed that at least 35 structural genes involved in the biosynthesis of volatile terpenes(VTs), volatile phenylpropanoid/benzenoids(VPBs),fatty acid-derived volatiles(FADVs), and indole were highly expressed in the flower-opening stage, both preharvest and postharvest, and are proposed to be important in endowing flower aroma. Additionally, at least 28 heat shock protein(HSP) and 11 β-glucosidase(BGLU) genes may be involved in the formation of floral fragrance. These findings provide insights into the formation of the floral fragrance of jasmine and will promote germplasm utilization for breeding improved jasmine varieties. | Chengzhe Zhou Chen Zhu Caiyun Tian Siyi Xie Kai Xu Linjie Huang Shengjing Wen Cheng Zhang Zhongxiong Lai Yuling Lin Yuqiong Guo | 2023 | Horticultural Plant Journal2023，9，6： | 0 |
| 6 | Achieving reliable and anti-collusive outsourcing computation and verification based on blockchain in 5G-enabled IoT显示文摘Widespread applications of 5G technology have prompted the outsourcing of computation dominated by the Internet of Things(IoT)cloud to improve transmission efficiency,which has created a novel paradigm for improving the speed of common connected objects in IoT.However,although it makes it easier for ubiquitous resource-constrained equipment that outsources computing tasks to achieve high-speed transmission services,security concerns,such as a lack of reliability and collusion attacks,still exist in the outsourcing computation.In this paper,we propose a reliable,anti-collusion outsourcing computation and verification protocol,which uses distributed storage solutions in response to the issue of centralized storage,leverages homomorphic encryption to deal with outsourcing computation and ensures data privacy.Moreover,we embed outsourcing computation results and a novel polynomial factorization algorithm into the smart contract of Ethereum,which not only enables the verification of the outsourcing result without a trusted third party but also resists collusion attacks.The results of the theoretical analysis and experimental performance evaluation demonstrate that the proposed protocol is secure,reliable,and more effective compared with state-of-the-art approaches. | Linjie Wang Youliang Tian Jinbo Xiong | 2022 | Digital Communications and Networks2022，8，5： | 0 |
| 7 | A potential game-based outsourcing computation framework for multiple calculators显示文摘Outsourcing computation enables a computationally weak client to outsource the computation of a function f to a more powerful but untrusted server.The traditional outsourcing computation model forbids communication between players,but it has little effect.Based on the game theory,this paper establishes an outsourcing computation model which is more in line with the actual scene.Firstly,we construct a structural mapping relationship between security outsourcing computation and the optimization problem.Secondly,by designing the individual potential function and the global potential function,the individual goal is consistent with the global goal to ensure the correctness of the calculation results.Finally,in the information exchange environment between calculators,we construct a Zero-determinant strategy to ensure that the calculator chooses the strategy according to the predetermined target. | Duo Zhang Youliang Tian Linjie Wang | 2022 | Digital Communications and Networks2022，8，5： | 0 |
| 8 | Design and Synthesis of Tri-substituted Chiral Pyrrolidin-2-one Derivatives as CCR4 Antagonists显示文摘一系列代替 tri 的 chiral pyrrolidin-2-one 衍生物作为 CC chemokine 受体 4 被设计了并且综合(CCR4 ) 对手。CCR4 的结构被相同建模造。不对称的合成被使用综合 R, R 配置 chiral pyrrolidin-2-one 支架。混合物的 stereoisomeric 配置被 2D 1H-1H 识别舒适光谱学和 1D NOESY 光谱学。这个方法比传统的 X 光检查单个水晶衍射更节俭、方便。另外,在这些混合物之间的相互作用和 N 终端 CCR4 的细胞外的尾巴用毛状的地区电气泳动被学习。CCR4 趋化性抑制效果在 CCR4-transfected HEK293 房间被测试。几混合物作为 CCR4 对手显示出有势力活动。在这些混合物之中, 1c 是最活跃的。它 CZE 实验结果的明显的有约束力的常数是(1. | Wei Sun Linjie Tian Hui Qi Dan Jiang Ying Wang Song Li Junhai Xiao Xiaohong Yang | 2013 | Chinese Journal of Chemistry2013，31，9： | 0 |
您所在的机构暂未开通此部分的功能，请联系管理员