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| 1 | The isolation and identification of apolipoprotein C-I in hormone-refractory prostate cancer using surface-enhanced laser desorption/ionization time-of-flight mass spectrometry显示文摘雄激素在前列腺癌症致病起一个中央作用,并且因此病人的大多数对雄激素剥夺治疗作出回应。然而,病人们与好攻击的前列腺癌症趋于到恶化,它是被称为荷尔蒙倔强。识别调停的蛋白质前进到荷尔蒙倔强的状态,我们为病人的 sera 介绍的质量使用了蛋白质薄片技术。这研究与变形荷尔蒙倔强的前列腺癌症包括了 16 个病人开始与雄激素剥夺被对待治疗。浆液样品在五个次点从每个病人被收集:点 A,预告的处理;削尖 B,在前列腺特定的抗原(PSA ) 的天底水平;点 C, PSA 失败;点 D,早荷尔蒙倔强的阶段;并且削尖 E,晚荷尔蒙倔强的阶段。用提高表面的激光解吸附作用 / 电离 time-of-flight 团 spectrometry,我们执行了病人的 sera 介绍的蛋白质质量并且识别了与疾病前进增加了的 6 640-Da 山峰。目标蛋白质部分被净化,并且由氨基酸定序,山峰作为 apolipoprotein C-I 的碎片被识别(ApoC -- 我) 。浆液 ApoC -- 我蛋白质层次与疾病前进增加了。在 immunohistochemical 分析上, ApoC -- 我蛋白质被发现局部性到荷尔蒙倔强的癌症房间的细胞质。在这研究,我们在浆液 ApoC 显示出增加 -- 我蛋白质在他们的前进期间在前列腺癌症病人铺平到荷尔蒙倔强的状态,它建议那 ApoC -- 我蛋白质与前列腺癌症的前进有关。然而, ApoC 的准确角色 -- 我在前列腺癌症致病是不清楚的,进一步的研究被要求。 | Kaori Yamamoto-Ishikawa Hiroyoshi Suzuki Masahiko Nezu Naoto Kamiya Takashi Imamoto Akira Komiya Kazuyuki Sogawa Takeshi Tomonaga Fumlo Nomur Tomohiko Ichikawa | 2009 | Asian Journal of Andrology2009，11，3： | 3 |
| 2 | SAP155-Mediated Splicing of FUSE-Binding Protein-Interacting Repressor Serves as a Molecular Switch for c-myc Gene Expression显示文摘 | Kazuyuki Matsushita Toshiko Kajiwara Mai Tamura Mamoru Satoh Nobuko Tanaka Takeshi Tomonaga Hisahiro Matsubara Hideaki Shimada Rei Yoshimoto Akihiro Ito Shuji Kubo Tohru Natsume David Levens Minoru Yoshida Fumio Nomura | 2012 | Molecular Cancer Research2012，，6： | 2 |
| 3 | Non-transmissible Sendai virus vector encoding c-myc suppressor FBP-interacting repressor for cancer therapy显示文摘AIM:To investigate a novel therapeutic strategy to target and suppress c-myc in human cancers using far up stream element(FUSE)-binding protein-interacting repressor(FIR).METHODS:Endogenous c-Myc suppression and apoptosis induction by a transient FIR-expressing vector was examined in vivo via a HA-tagged FIR(HA-FIR)expression vector.A fusion gene-deficient,non-transmissible,Sendai virus(SeV)vector encoding FIR cDNA,SeV/dF/FIR,was prepared.SeV/dF/FIR was examined for its gene transduction efficiency,viral dose dependency of antitumor effect and apoptosis induction in HeLa(cervical squamous cell carcinoma)cells and SW480(colon adenocarcinoma)cells.Antitumor efficacy in a mouse xenograft model was also examined.The molecular mechanism of the anti-tumor effect and c-Myc suppression by SeV/dF/FIR was examined using Spliceostatin A(SSA),a SAP155 inhibitor,or SAP155siRNA which induce c-Myc by increasing FIR△exon2 in HeLa cells.RESULTS:FIR was found to repress c-myc transcription and in turn the overexpression of FIR drove apoptosis through c-myc suppression.Thus,FIR expressing vectors are potentially applicable for cancer therapy.FIR is alternatively spliced by SAP155 in cancer cells lacking the transcriptional repression domain within exon 2(FIR△exon2),counteracting FIR for c-Myc protein expression.Furthermore,FIR forms a complex with SAP155 and inhibits mutual well-established functions.Thus,both the valuable effects and side effects of exogenous FIR stimuli should be tested for future clinical application.SeV/dF/FIR,a cytoplasmic RNA virus,was successfully prepared and showed highly efficient gene transduction in in vivo experiments.Furthermore,in nude mouse tumor xenograft models,SeV/dF/FIR displayed high antitumor efficiency against human cancer cells.SeV/dF/FIR suppressed SSA-activated c-Myc.SAP155 siRNA,potentially produces FIR△exon2,and led to c-Myc overexpression with phosphorylation at Ser62.HA-FIR suppressed endogenous c-Myc expression and induced apoptosis in HeLa and SW480 cells.A c-myc transcriptional suppressor FIR expressing SeV/dF/FIR showed high gene transduction efficiency with significant antitumor effects and apoptosis induction in HeLa and SW480 cells.CONCLUSION:SeV/dF/FIR showed strong tumor growth suppression with no significant side effects in an animal xenograft model,thus SeV/dF/FIR is potentially applicable for future clinical cancer treatment. | Kazuyuki Matsushita Hideaki Shimada Yasuji Ueda Makoto Inoue Mamoru Hasegawa Takeshi Tomonaga Hisahiro Matsubara Fumio Nomura | 2014 | World Journal of Gastroenterology2014，20，15： | 2 |
| 4 | Interactions between SAP155 and FUSE-Binding Protein-Interacting Repressor Bridges c-Myc and P27Kip1 Expression显示文摘 | Kazuyuki Matsushita Mai Tamura Nobuko Tanaka Takeshi Tomonaga Hisahiro Matsubara Hideaki Shimada David Levens Liusheng He Juhong Liu Minoru Yoshida Fumio Nomura | 2013 | Molecular Cancer Research2013，，7： | 1 |
| 5 | Structural health monitoring of cracked aircraft panels repaired with bonded patches using fiber Bragg grating sensors显示文摘 | HIDEKI S SHIN-ETSU F TOMONAGA O | 2006 | Applied Composite Materials2006，13，2： | 1 |
| 6 | Identification of novel and downregulated biomarkers for alcoholism by surface enhanced laser desorption/ionization-mass spectrometry显示文摘 | Nomura F Tomonaga T Sogawa K | 2004 | Proteomies2004，4，4： | 1 |
| 7 | Focal cervical poliopathy causing juvenile muscular atrophy of dsitai upper extremity:a pathological study 显示文摘 | HIRAYAMA K TOMONAGA M KITANO K | 1987 | Neurol Neurosurg Psychiatry1987，50，： | 1 |
| 8 | Indium-tin oxide coatings via chemical solution deposition 显示文摘 | Tomonaga H Morimoto T | 2001 | Thin Solid Films2001，392，： | 1 |
| 9 | Focal cervical poliopathy causing juvenile muscular atrophy of distal upper extremity : a pathological study 显示文摘 | Hirayama K Tomonaga M Kitano K | 1987 | J Neurol Neurosurg Psychiatry1987，50，3： | 1 |
| 10 | Discrepant expression of membrane and soluble isoforms of Fas (CD95/APO-1) in adult T-cell leukaemia:soluble Fas isoform is an independent risk factor for prognosis显示文摘 | KAMIHIRA S YAMADA Y TOMONAGA M | 1999 | Br J Haematol1999，107，4： | 1 |
| 11 | Focal cervicalpoliopathy causing juvenilemuscular atrophy of distal upper extremity:a pathological study显示文摘 | Hirayama K Tomonaga M Kitano K | | 0，，03： | 1 |
| 12 | Study of the welding parameters of the digital inverter type pulse MIG welding machine-Case of the welding pcfforrnancc qualification Flat position 显示文摘 | Saito Shinji Tomonaga Naoya Kuwano Ryoichi | 2012 | Keikinzoku Yosctsu/Journa[ of Light Metal Welding and Construction2012，50，7： | 1 |
| 13 | Possible involvement of aryl hydrocarbon receptor (AhR) in adult T-cell leukemia (ATL) leukemogenesis: constitutive activation of AhR in ATL显示文摘 | Toshihisa Hayashibara Yasuaki Yamada Naoki Mori Hitomi Harasawa Kazuyuki Sugahara Takayuki Miyanishi Shimeru Kamihira Masao Tomonaga | 2002 | Biochemical and Biophysical Research Communications2002，，1： | 1 |
| 14 | Identifica- tion of altered protein expression and posttranslational modi- fications in primary colorectal cancer by using agarose two-di- mensional gel electrophoresis显示文摘 | Tomonaga T Matsushita K Yamaguchi S | 2009 | Clin Cancer Res2009，10，6： | 1 |
| 15 | Focal cervical poliopathy causing juvenile muscular atrophy of distal upper extremity:a pathological study 显示文摘 | Hirayama K Tomonaga M Kitano K | 1987 | J Neurol Neurosurg Psychiatry1987，50，3： | 1 |
| 16 | Intracerebroventricu- lar injection of vasoactive intestinal peptide and pituitary adenyl- ate cyclase-activating polypeptide inhibits feeding in chicks显示文摘 | Tachibana T Saito S Tomonaga S | 2003 | Neurosci Lett2003，339，： | 1 |
| 17 | An essential role of alternative splicing of c-myc suppressor FUSE-binding protein-interacting repressor in carcinogenesis显示文摘 | Matsushita K Tomonaga T Shimada H Shioya A Higashi M Matsubara H | 2006 | Cancer Res2006，66，： | 1 |
| 18 | The detection of the mRNAs of procollagen types I,II and III in human fetal fingers by in situ oligo mucleotide probes显示文摘 | Nefryer J Tomonaga A | 1995 | Histochemical J1995，27，30： | 1 |
| 19 | Numerical simulation of inerlaminar damage propagation in CFRP cross-ply laminates under transverse loading 显示文摘 | Masaaki N Tomonaga O Nobuo T | 2007 | International Journal of Solids and Structures2007，44，： | 1 |
| 20 | Reaction of polycyclic aromatic hydrocarbons adsorbed on silica in aqueous chlorine 显示文摘 | Nakamura H Tomonaga Y Miyata K | 2007 | Environmental science & technology2007，41，11： | 1 |
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