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| 1 | One-step generation of complete gene knockout mice and monkeys by CRISPR/Cas9-mediated gene editing with multiple sgRNAs显示文摘CRISPR/Cas9 系统是一个有效编辑基因的方法,但是编辑基因的动物的多数显示出 mosaicism,与编辑仅仅在房间的部分发生。这里,我们证明那单个基因或多重基因能被 Cas9 mRNA 和多重邻近的单个指南的 RNA 的 zygotic 注射完全在老鼠和猴子胚胎击倒(spaced 10-200 bp 分开) 指向仅仅一把单个钥匙每基因的 exon。个别地在 Y 染色体上跟随八基因的指向的删除的 F0 老鼠的 Phenotypic 分析在产生猛烈老鼠表明了这条途径的坚韧性。重要地,这条途径在高效率交付完全的基因大美人(Arntl 上的 100% 并且 91% 在 Prrt2 上) 在猴子胚胎。最后,我们能在一个单个步骤产生一只完全的 Prrt2 猛烈猴子,表明在很快建立编辑基因的猴子模型的这条途径的实用性。 | Erwei Zuo Yi-Jun Cai Kui Li Yu Wei Bang-An Wang Yidi Sun Zhen Liu Jiwei Liu Xinde Hu Wei Wei Xiaona Huo Linyu Shi Cheng Tang Dan Liang Yan Wang Yan-Hong Nie Chen-Chen Zhang Xuan Yao Xing Wang Changyang Zhou Wenqin Ying Qifang Wang Ren-Chao Chen Qi Shen Guo-Liang Xu Jinsong Li Qiang Sun Zhi-Qi Xiong Hui Yang | 2017 | Cell Research2017，27，7： | 21 |
| 2 | CasRx-mediated RNA targeting prevents choroidal neovascularization in a mouse model of age-related macular degeneration显示文摘RNA-targeting CRISPR system Cas13 offers an efficient approach for manipulating RNA transcripts in vitro.In this perspective,we provide a proof-of-concept demonstration that Cas13-mediated Vegfa knockdown in vivo could prevent the development of laser-induced CNV in mouse model of Age-related macular degeneration. | Changyang Zhou Xinde Hu Cheng Tang Wenjia Liu Shaoran Wang Yingsi Zhou Qimeng Zhao Qiyu Bo Linyu Shi Xiaodong Sun Haibo Zhou Hui Yang | 2020 | National Science Review2020，7，5： | 2 |
| 3 | Facile synthesis of PDMAEMA-coated hollow mesoporous silica nanoparticles and their pH-responsive controlled release显示文摘 | Faqi Yu Xinde Tang Meishan Pei | 2013 | Microporous and Mesoporous Materials2013，，： | 1 |
| 4 | Disruption of splicing-regulatory elements using CRISPR/Cas9 to rescue spinal muscular atrophy in human iPSCs and mice显示文摘We here report a genome-editing strategy to correct spinal muscular atrophy(SMA).Rather than directly targeting the pathogenic exonic mutations,our strategy employed Cas9 and guide-sg RNA for the targeted disruption of intronic splicing-regulatory elements.We disrupted intronic splicing silencers(ISSs,including ISS-N1 and ISS+100)of survival motor neuron(SMN)2,a key modifier gene of SMA,to enhance exon 7 inclusion and full-length SMN expression in SMA iPSCs.Survival of splicing-corrected iPSC-derived motor neurons was rescued with SMN restoration.Furthermore,co-injection of Cas9 mRNA from Streptococcus pyogenes(SpCas9)or Cas9 from Staphylococcus aureus(SaCas9)alongside their corresponding sgRNAs targeting ISS-N1 into zygotes rescued 56%and 100%of severe SMA transgenic mice(Smn-/-,SMN2tg/-).The median survival of the resulting mice was extended to>400 days.Collectively,our study provides proof-of-principle for a new strategy to therapeutically intervene in SMA and other RNA-splicing-related diseases. | Jin-Jing Li Xiang Lin Cheng Tang Ying-Qian Lu Xinde Hu Erwei Zuo He Li Wenqin Ying Yidi Sun Lu-Lu Lai Hai-Zhu Chen Xin-Xin Guo Qi-Jie Zhang Shuang Wu Changyang Zhou Xiaowen Shen Qifang Wang Min-Ting Lin Li-Xiang Ma Ning Wang Adrian R.Krainer Linyu Shi Hui Yang Wan-Jin Chen | 2020 | National Science Review2020，7，1： | 1 |
| 5 | Triple stimuli‐responsive amphiphilic glycopolymer显示文摘 | Wenjuan Guo Tieshi Wang Xinde Tang Qun Zhang Faqi Yu Meishan Pei | 2014 | J Polym Sci Part A: Polym Chem2014，，15： | 1 |
| 6 | 显示文摘 | Tang Xinde Zhang Qizhen Fan Xinghe | 2004 | Chin J Chem2004，22，： | 1 |
| 7 | 显示文摘 | Tang Xinde Zhang Qizhen Li Aixiang | 2004 | Chin J Chem2004，22，： | 1 |
| 8 | 显示文摘 | Tang Xinde Zhang Qizhen Zhou Qifeng | 2003 | Chin J Org Chem2003，23，： | 1 |
| 9 | 显示文摘 | Tang Xinde Zhang Qizhen Hou Zhaosheng | 2004 | Chin J Org Chem2004，24，： | 1 |
| 10 | Photocatalytic Splitting of Water Over a Novel Visible-Light-Response Photocatalyst Nd2InTaO7显示文摘 | Xinde Tang Hongqi Ye Zhi Zhao Hui Liu Chenxia Ma | 2009 | Catalysis Letters (-)2009，，3： | 1 |
| 11 | 显示文摘 | Tang Xinde Cao Jun Zhang Qizhen | 2007 | Journal of Functional Materials2007，38，8： | 1 |
| 12 | Aluminum pigment encapsulated by in situ copolymerization of styrene and maleic aeid 显示文摘 | HUI LIU HONGQI YE XINDE TANG | 2007 | Original Research Article Applied Surface Science2007，254，2： | 1 |
| 13 | Photocatalytic Splitting of Water Over a Novel Visible-Light-Response Photocatalyst Nd2InTaO7显示文摘 | Xinde Tang Hongqi Ye Zhi Zhao Hui Liu Chenxia Ma | 2009 | Catalysis Letters (-)2009，，3： | 1 |
| 14 | Comparison of Time Interval Statistic and Pulse Shape Discrimination in Fast Neutron Detection Method with Liquid Scintillation Detector Loaded Gd显示文摘Neutron usually appears with gamma,which is requiring the detector has the capability of n gamma discrimination.Pulse shape discrimination(PSD)is a common approach of n,gamma judgment,but required a complex process to select a suitable discrimination factor,and poor performance in the low energy range.A method based on the time interval of adjacent Signals was disrupted and adopted to compare to pulse shape discrimination in the fast neutron detection method.A good agreement between the adopted method and PSD method was achieved,including the total count ratio and neutron count ratio.The comparison proves the correctness of the theoretical derivation and validates the method in practice.The advantages and limitations of the method based on time Interval of adjacent signals were analyzed.The method provides an effective way to confirm the calibration of the neutron detection of a liquid scintillation detector.Also,as a simple way only requiring the time information about events,the described method has large application potential in some case of fast neutron flux or intensity measurement. | Mohammed Adel Al-jumaili Qiang Du Jingjun Zhu Xinde Lin Wenbin Lin Changjian Tang Mustafa Raad Kadhim Haoyang Xing Shukui Liu | 2017 | 应用物理前沿(中英文版)2017，5，1： | 0 |
| 15 | Novel Cross-Linked Conducting Polythiophene with Yellow-Green-Light-Emitting Properties and Good Thermal Stability显示文摘有经由一个烷基分隔符属于聚合物脊梁的 polymethacrylate 的新奇结合的 polythiophene 衍生物成功地被综合。代替 methacrylate 的 thiophene 单体, 3-( 己基的 methacrylate ) thiophene 被准备并且由免费激进的聚合的 polymerized,由电气化学的聚合列在后面。是的产生聚合物 yellow-green-light emitter,在 photoelectronics 有潜在的应用区域。 | Lai, Cunyuan Guo, Wenjuan Tang, Xinde Zhang, Guangyou Pan, Qiwei Pei, Meishan | 2011 | Chinese Journal of Chemistry2011，29，9： | 0 |
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